LOCUS       sequence-342351-        3631 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multisite Gateway 3' entry clone for fusions onto the N-terminus of 
            TurboID. Parton lab clone JSS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3631)
  AUTHORS   Xiong Z, Lo HP, McMahon KA, Martel N, Jones A, Hill MM, Parton RG, 
            Hall TE
  TITLE     In vivo proteomic mapping through GFP-directed proximity-dependent 
            biotin labelling in zebrafish.
  JOURNAL   Elife. 2021 Feb 16;10. pii: 64631. doi: 10.7554/eLife.64631.
  PUBMED    33591275
REFERENCE   2  (bases 1 to 3631)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2021-02-16"; volume: "10. pii: 64631. doi"; pages: " 
            10.7554/eLife.64631"
COMMENT     Sequence Label: p3E-TurboID
FEATURES             Location/Qualifiers
     source          1..3631
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(624..748)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1707..1736
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    complement(1740..1835)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1872..1891)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1873..1891)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1896..1912)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1896..1912)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2014..2033)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2025..2834
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2092..2111)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2981..3569
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3470..3489
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggccctg cagctctaga gctcgaattc tacaggtcac
      601 taataccatc taagtagttg gttcatagtg actgcatatg ttgtgtttta cagtattatg
      661 tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt
      721 ctcgttcaac tttcttgtac aaagtggtga aagacaatac agtccccttg aagctgattg
      781 cccttctggc aaatggcgaa ttccacagcg gggagcaact gggcgaaacg ttgggcatga
      841 gtagagcagc gataaacaaa catatacaaa cgctgaggga ttggggtgta gacgtgttca
      901 ccgttccggg caagggctat agtttgcccg aaccgatacc acttctgaac gccaaacaaa
      961 tattgggtca actggatgga ggatcagtag cagttcttcc tgtggtcgac tctactaacc
     1021 aatatctgct ggatcggatc ggggaactga aatctggcga tgcatgcata gctgagtacc
     1081 agcaggctgg acggggatct cgaggaagga aatggttttc cccgtttggg gccaacctct
     1141 atctctctat gttctggagg ttgaaacgtg gccctgctgc catcggcttg gggcctgtca
     1201 ttggtattgt tatggcggag gcgttgcgta aacttggagc ggataaagtg agggttaaat
     1261 ggccaaacga tttgtatctt caagaccgca agttggctgg tatactggta gaactcgcag
     1321 ggattacagg agatgcggct cagattgtga tcggtgccgg tattaacgtc gcgatgcgta
     1381 gggtagagga gtccgtggtc aatcagggtt ggattacgct gcaagaggct ggaatcaatc
     1441 tcgacagaaa cacacttgca gccacgctta ttcgggaatt gagggctgct cttgaacttt
     1501 ttgaacagga agggttggca ccttacctgc cgcgctggga aaagctggat aatttcataa
     1561 atcggcccgt caagctcata atcggggaca aggaaatttt cggcatatca cgcggcatcg
     1621 acaagcaggg ggcactgctg ttggaacagg acggagtcat aaagccctgg atgggtggtg
     1681 agattagcct tcgatctgcg gagaaagagc agaaactgat aagcgaagag gacctgtaac
     1741 aactttatta tacaaagttg gcattataaa aaagcattgc ttatcaattt gttgcaacga
     1801 acaggtcact atcagtcaaa ataaaatcat tatttggagc tccatggtag cgttaacgcg
     1861 gccgcgatat cccctatagt gagtcgtatt acatggtcat agctgtttcc tggcagctct
     1921 ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgaa
     1981 caataaaact gtctgcttac ataaacagta atacaagggg tgttatgagc catattcaac
     2041 gggaaacgtc gaggccgcga ttaaattcca acatggatgc tgatttatat gggtataaat
     2101 gggctcgcga taatgtcggg caatcaggtg cgacaatcta tcgcttgtat gggaagcccg
     2161 atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg
     2221 agatggtcag actaaactgg ctgacggaat ttatgcctct tccgaccatc aagcatttta
     2281 tccgtactcc tgatgatgca tggttactca ccactgcgat ccccggaaaa acagcattcc
     2341 aggtattaga agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc
     2401 tgcgccggtt gcattcgatt cctgtttgta attgtccttt taacagcgat cgcgtatttc
     2461 gtctcgctca ggcgcaatca cgaatgaata acggtttggt tgatgcgagt gattttgatg
     2521 acgagcgtaa tggctggcct gttgaacaag tctggaaaga aatgcataaa cttttgccat
     2581 tctcaccgga ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg
     2641 aggggaaatt aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg
     2701 atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt
     2761 ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatgctcg
     2821 atgagttttt ctaatcagaa ttggttaatt ggttgtaaca ctggcagagc attacgctga
     2881 cttgacggga cggcgcaagc tcatgaccaa aatcccttaa cgtgagttac gcgtcgttcc
     2941 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     3001 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     3061 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     3121 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     3181 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     3241 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     3301 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     3361 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     3421 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     3481 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     3541 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     3601 tggccttttg ctggcctttt gctcacatgt t
//
