LOCUS       sequence-342352-        4275 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  donor plasmid for SapTrap assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4275)
  AUTHORS   Kroll JR, Remmelzwaal S, Boxem M
  TITLE     CeLINC, a fluorescence-based protein-protein interaction assay in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2021 Dec 10;219(4). pii: 6380436. doi: 
            10.1093/genetics/iyab163.
  PUBMED    34849800
REFERENCE   2  (bases 1 to 4275)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics.";
            date: "2021-12-10"; volume: "219(4). pii: 6380436. doi"; pages: " 
            10.1093/genetics/iyab163"
COMMENT     Sequence Label: pJRK247
FEATURES             Location/Qualifiers
     source          1..4275
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(214..231)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(385..973)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(465..484)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(1342..1361)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1353..2168
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1426..1445)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     CDS             complement(2169..4073)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MITNSRSSSKMKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPV
                     FIWCPEEEGQFYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTG
                     ATKVVFNHLYDPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNS
                     YWKKCLDMSIESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSP
                     GWSNADKLLNEFIEKQLIDYAKNSKKVSLNIYILTNPDYLNFQVVGNSTSLLSPYLHFG
                     EISVRHVFQCARMKQIIWARDKNSEGEESADLFLRGIGLREYSRYICFNFPFTHEQSLL
                     SHLRFFPWDADVDKFKAWRQGRTGYPLVDAGMRELWATGWMHNRIRVIVSSFAVKFLLL
                     PWKWGMKYFWDTLLDADLECDILGWQYISGSIPDGHELDRLDNPALQGAKYDPEGEYIR
                     QWLPELARLPTEWIHHPWDAPLTVLKASGVELGTNYAKPIVDIDTARELLAKAISRTRE
                     AQIMIGAAGGGSGGGSGGTRRAYSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLN
                     RLAAHPPFASWRNSEEARTDRPSQQLRSLNGECDLFNKAAVPSSQRNALPVLQPINQF"
     primer_bind     2372..2394
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2386..2403
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2387..2403
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4074..4090)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4074..4090)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4087..4109)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4098..4114
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4122..4128,4129..4146,4147..4152))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4167..4188
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ggtttgcgta ttggcgaact tttgctgagt tgaaggatca gatcacgcat cttcccgaca
       61 acgcagaccg ttccgtggca aagcaaaagt tcaaaatcag taaccgtcag tgccgataag
      121 ttcaaagtta aacctggtgt tgataccaac attgaaacgt tgatcgaaaa cgcgctgaaa
      181 aacgctgctg aatgtgcgag cttcttccgc ttcctcgctc actgactcgc tgcgctcggt
      241 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
      301 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
      361 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
      421 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
      481 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
      541 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
      601 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
      661 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
      721 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
      781 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
      841 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
      901 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
      961 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaactc
     1021 cgtcgagagg tctgcctcgt gaagaaggtg ttgctgactc ataccaggcc tgaatcgccc
     1081 catcatccag ccagaaagtg agggagccac ggttgatgag agctttgttg taggtggacc
     1141 agttggtgat tttgaacttt tgctttgcca cggaacggtc tgcgttgtcg ggaagatgcg
     1201 tgatctgatc cttcaactca gcaaaagttc gatttattca acaaagccac gttgtgtctc
     1261 aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgaaca ataaaactgt
     1321 ctgcttacat aaacagtaat acaaggggtg ttatgagcca tattcaacgg gaaacgtctt
     1381 gctcgaagcc gcgattaaat tccaacatgg atgctgattt atatgggtat aaatgggctc
     1441 gcgataatgt cgggcaatca ggtgcgacaa tctatcgatt gtatgggaag cccgatgcgc
     1501 cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg
     1561 tcagactaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat tttatccgta
     1621 ctcctgatga tgcatggtta ctcaccactg cgatccccgg gaaaacagca ttccaggtat
     1681 tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc
     1741 ggttgcattc gattcctgtt tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg
     1801 ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt gatgacgagc
     1861 gtaatggctg gcctgttgaa caagtctgga aagaaatgca taagcttttg ccattctcac
     1921 cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt gacgagggga
     1981 aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac caggatcttg
     2041 ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa
     2101 aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg ctcgatgagt
     2161 ttttctaatc agaattggtt aattggttgt aacactggca gagcattacg ctgacttgac
     2221 gggacggcgg ctttgttgaa taaatcgcat tcgccattca ggctgcgcaa ctgttgggaa
     2281 gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca
     2341 aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc
     2401 agtgccaagc ttgcatgcct gcaggtcgac tctagaggat ccccgggtac cgagtaagct
     2461 cttcgcgtac ctccggatcc acccccagat ccgccaccag cggctccgat catgatctga
     2521 gcctcgcggg tgcgagagat agccttagca agaagctcac gagcggtgtc gatatcaacg
     2581 attggcttag cgtagttagt tccaagctca actccggaag ccttaaggac ggtaagtggg
     2641 gcatcccatg ggtgatggat ccattcagtt ggaaggcgag caagctctgg aagccattgg
     2701 cggatgtatt ctccttctgg atcgtactta gctccttgga gagctgggtt gtcaagacgg
     2761 tcaagctcgt gtccatctgg gatagatcca gagatgtact gccatccaag gatatcgcac
     2821 tcaaggtcag cgtcaagaag ggtgtcccag aaatatttca ttccccattt ccatggaaga
     2881 agaaggaact taacagcgaa ggaggaaacg ataacacgga tgcggttatg catccatcca
     2941 gtagcccaaa gctcacgcat tccagcatcg acaagtgggt atccagtgcg tccttggcgc
     3001 caagccttga acttatcgac atcagcatcc catgggaaga agcgaaggtg agaaagaagg
     3061 gattgctcat gagtgaatgg gaagttgaaa caaatgtagc gagagtactc gcgaagtccg
     3121 attccgcgaa ggaaaaggtc agcggactcc tctccttcag agttcttatc acgagcccag
     3181 atgatctgct tcatgcgagc gcattggaaa acgtgacgaa cggagatttc tccgaagtga
     3241 aggtatggag aaagaaggga ggtggagttt ccaacaacct gaaaatttaa ataatcaggg
     3301 ttagttagta tatatatgtt taaacttacc ttcttagagt tcttagcgta atcgataagt
     3361 tgcttttcga tgaattcgtt aagaagcttg tcagcgttag accatcctgg agaccaagca
     3421 cgggtaagaa gggcgttaga tggcttctca gcctcgtttt caagtccaag ctcctcgata
     3481 gagcaagccc agatagcctc agcagcagcg gtgattggca taaggcgcca tggtggtgga
     3541 agcataacgg actcgataga catatcaagg cacttcttcc agtaagaatt gaaagaagtg
     3601 aatggctttc ccttttcaca gtaaatttcc catggttcgt aaagaagatc tccgttgtag
     3661 gattgaacag agattccacg ctcaacaagc ttctccttaa cggtgtgatc acgaacaagg
     3721 gagactggat cgtaaaggtg gttgaaaaca accttggtag ctccggtaac acggatgcaa
     3781 tcaaggatag cggagatagt gttgtgagtc ttgataagag taaggtcgga tccaagggcc
     3841 ttaagggatt gggaaaggtg ggcaagagat tgcttcatcc accagcgaga agcgcgtcct
     3901 gggtagaact gtccttcctc ttctgggcac caaatgaaaa ctgggaaaac agatccctcg
     3961 tgagcagcag cagcaagagc tgggttatcc tcgatacgaa gatcacgacg gaaccaaacg
     4021 atagtcttct tatccatctt catcttcgaa gagctcctcg aattcgtaat catgtcatag
     4081 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     4141 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     4201 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     4261 cgcgcgggga gaggc
//
