LOCUS       sequence-342360-        4855 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid contains the Sindbis capsid gene and can be converted to 
            mRNA for initiating Sindbis virus packaging. See Resource 
            Information section for annotated plasmid sequence..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4855)
  AUTHORS   English JG, Olsen RHJ, Lansu K, Patel M, White K, Cockrell AS, Singh
            D, Strachan RT, Wacker D, Roth BL
  TITLE     VEGAS as a Platform for Facile Directed Evolution in Mammalian 
            Cells.
  JOURNAL   Cell. 2019 Jul 2. pii: S0092-8674(19)30622-1. doi: 
            10.1016/j.cell.2019.05.051.
  PUBMED    31280962
REFERENCE   2  (bases 1 to 4855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2019 
            Jul 2. pii: S0092-8674(19)30622-1. doi: 10.1016/j.cell.2019.05.051."
COMMENT     Sequence Label: pSin Capsid
FEATURES             Location/Qualifiers
     source          1..4855
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..19
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1..18
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        3044..3148
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3149..3217,3218..4009)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3367..3386)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4180..4768
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4669..4688
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atttaggtga cactatagat tggcggcgta gtacacacta ttgaatcaaa cagccgacca
       61 attgcactac catcacaatg gagaagccag tagttaacgt agacgtagac cctcagagtc
      121 cgtttgtcgt gcaactgcaa aagagcttcc cgcaatttga ggtagtagca cagcaggtca
      181 ctccaaatga ccatgctaat gccagagcat tttcgcatct ggccagtaaa ctgatcgagc
      241 tggaggttcc taccacagcg acgattttgg acataggcag cgcaccggct cgtagaatgt
      301 tttccgagca ccagtaccat tgcgtttgcc ccatgcgtag tccagaagac ccggaccgca
      361 tgatgaaata tgccagcaaa ctggcggaaa aagcatgtaa gattacaaac aagaacttgc
      421 atgagaagat caaggacctc cggaccgtac ttgatacacc ggatgctgaa acgccatcac
      481 tctgcttcca caacgatgtt acctgcaaca cgcgtgccga gtactccgtc atgcaggacg
      541 tgtacatcaa cgctcccgga actatttacc accaggctat gaaaggcgtg cggaccctgt
      601 actggattgg cttcgacacc acccagttca tgttctcggc tatggcaggt tcgtaccctg
      661 catacaacac caactgggcc gacgaaaaag tccttgaagc gcgtaacatc ggactctgca
      721 gcacaaagct gagtgaaggc aggacaggaa agttgtcgat aatgaggaag aaggagttga
      781 agcccatcgc ctcgttcgac aaaagccaag acgacgctat ggcgttaacc ggcctgatga
      841 tcttggaaga cctgggtgtg gaccaaccac tactcgactt gatcgagtgc gcctttggag
      901 aaatatcatc cacccatctg cccacgggta cccgtttcaa attcggggcg atgatgaaat
      961 ccggaatgtt cctcacgctc tttgtcaaca cagttctgaa tgtcgttatc gccagcagag
     1021 tattggagga gcggcttaaa acgtccaaat gtgcagcatt tatcggcgac gacaacatta
     1081 tacacggagt agtatctgac aaagaaatgg ctgagaggtg tgccacctgg ctcaacatgg
     1141 aggttaagat cattgacgca gtcatcggcg agagaccacc ttacttctgc ggtggattca
     1201 tcttgcaaga ttcggttacc tccacagcgt gtcgcgtggc ggaccccttg aaaaggctgt
     1261 ttaagttggg taaaccgctc ccagccgacg atgagcaaga cgaagacaga agacgcgctc
     1321 tgctagatga aacaaaggcg tggtttagag taggtataac agacacctta gcagtggccg
     1381 tggcaactcg gtatgaggta gacaacatca cacctgtcct gctggcattg agaacttttg
     1441 cccagagcaa aagagcattt caagccatca gaggggaaat aaagcatctc tacggtggtc
     1501 ctaaatagtc agcatagtac atttcatctg actaatacca caacaccacc accatgaata
     1561 gaggattctt taacatgctc ggccgccgcc ccttcccagc ccccactgcc atgtggaggc
     1621 cgcggagaag gaggcaggcg gccccgatgc ctgcccgcaa tgggctggct tcccaaatcc
     1681 agcaactgac cacagccgtc agtgccctag tcattggaca ggcaactaga cctcaaaccc
     1741 cacgcccacg cccgccgccg cgccagaaga agcaggcgcc aaagcaacca ccgaagccga
     1801 agaaaccaaa aacacaggag aagaagaaga agcaacctgc aaaacccaaa cccggaaaga
     1861 gacagcgtat ggcacttaag ttggaggccg acagactgtt cgacgtcaaa aatgaggacg
     1921 gagatgtcat cgggcacgca ctggccatgg aaggaaaggt aatgaaacca ctccacgtga
     1981 aaggaactat tgaccaccct gtgctatcaa agctcaaatt caccaagtcg tcagcatacg
     2041 acatggagtt cgcacagttg ccggtcaaca tgagaagtga ggcgttcacc tacaccagtg
     2101 aacaccctga agggttctac aactggcacc acggagcggt gcagtatagt ggaggcagat
     2161 ttaccatccc ccgcggagta ggaggcagag gagacagtgg tcgtccgatt atggataact
     2221 caggccgggt tgtcgcgata gtcctcggag gggctgatga gggaacaaga accgcccttt
     2281 cggtcgtcac ctggaatagc aaagggaaga caatcaagac aaccccggaa gggacagaag
     2341 agtggtctgc tgcaccactg gtcacggcca tgtgcttgct tggaaacgtg agcttcccat
     2401 gcaatcgccc gcccacatgc tacacccgcg aaccatccag agctctcgac atcctcgaag
     2461 agaacgtgaa ccacgaggaa ttccaagccg ccatctcaaa aacttcatgg agttggctgt
     2521 ttgccctttt cggcggcgcc tcgtcgctat taattatagg acttatgatt tttgcttgca
     2581 gcatgatgct gactagcaca cgaagatgac cgctacgccc caatgacccg accagcaaaa
     2641 ctcgatgtac ttccgaggaa ctgatgtgca taatgcatca ggctggtata ttagatcccc
     2701 gcttaccgcg ggcaatatag caacaccaaa actcgacgta tttccgagga agcgcagtgc
     2761 ataatgctgc gcagtgttgc caaataatca ctatattaac catttattca gcggacgcca
     2821 aaactcaatg tatttctgag gaagcatggt gcataatgcc atgcagcgtc tgcataactt
     2881 tttattattt cttttattaa tcaacaaaat tttgttttta acatttcaaa aaaaaaaaaa
     2941 aaaaaaaaaa aaaaaaaaaa aaaaaaaagt ttaaactcta gagctcgagg ggaattaatt
     3001 cttgaagacg aaagggccag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     3061 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     3121 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     3181 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     3241 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     3301 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     3361 agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg
     3421 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     3481 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     3541 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     3601 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     3661 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     3721 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     3781 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     3841 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     3901 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     3961 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     4021 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     4081 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     4141 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     4201 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     4261 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     4321 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     4381 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     4441 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     4501 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     4561 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     4621 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     4681 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     4741 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcgagctcgt atggacatat
     4801 tgtcgttaga acgcggctac aattaataca taaccttatg tatcatacac atacg
//
