LOCUS       sequence-342435-        4275 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  donor plasmid for SapTrap assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4275)
  AUTHORS   Kroll JR, Remmelzwaal S, Boxem M
  TITLE     CeLINC, a fluorescence-based protein-protein interaction assay in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2021 Dec 10;219(4). pii: 6380436. doi: 
            10.1093/genetics/iyab163.
  PUBMED    34849800
REFERENCE   2  (bases 1 to 4275)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics.";
            date: "2021-12-10"; volume: "219(4). pii: 6380436. doi"; pages: " 
            10.1093/genetics/iyab163"
COMMENT     Sequence Label: pJRK109
FEATURES             Location/Qualifiers
     source          1..4275
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(316..335)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             327..1142
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(400..419)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1346..1368
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1360..1377
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1361..1377
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3048..3064)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3048..3064)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3061..3083)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3072..3088
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3096..3102,3103..3120,3121..3126))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3141..3162
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3463..3480)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3634..4222)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3714..3733)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaggtctgcc tcgtgaagaa ggtgttgctg actcatacca ggcctgaatc gccccatcat
       61 ccagccagaa agtgagggag ccacggttga tgagagcttt gttgtaggtg gaccagttgg
      121 tgattttgaa cttttgcttt gccacggaac ggtctgcgtt gtcgggaaga tgcgtgatct
      181 gatccttcaa ctcagcaaaa gttcgattta ttcaacaaag ccacgttgtg tctcaaaatc
      241 tctgatgtta cattgcacaa gataaaaata tatcatcatg aacaataaaa ctgtctgctt
      301 acataaacag taatacaagg ggtgttatga gccatattca acgggaaacg tcttgctcga
      361 agccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata
      421 atgtcgggca atcaggtgcg acaatctatc gattgtatgg gaagcccgat gcgccagagt
      481 tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac
      541 taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg
      601 atgatgcatg gttactcacc actgcgatcc ccgggaaaac agcattccag gtattagaag
      661 aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc
      721 attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg
      781 cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg
      841 gctggcctgt tgaacaagtc tggaaagaaa tgcataagct tttgccattc tcaccggatt
      901 cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa
      961 taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc
     1021 tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg
     1081 gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct
     1141 aatcagaatt ggttaattgg ttgtaacact ggcagagcat tacgctgact tgacgggacg
     1201 gcggctttgt tgaataaatc gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
     1261 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
     1321 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
     1381 aagcttgcat gcctgcaggt cgactctaga ggatccccgg gtaccgagga gctcttcgaa
     1441 gatgaagatg gataagaaga ctatcgtttg gttccgtcgt gatcttcgta tcgaggataa
     1501 cccagctctt gctgctgctg ctcacgaggg atctgttttc ccagttttca tttggtgccc
     1561 agaagaggaa ggacagttct acccaggacg cgcttctcgc tggtggatga agcaatctct
     1621 tgcccacctt tcccaatccc ttaaggccct tggatccgac cttactctta tcaagactca
     1681 caacactatc tccgctatcc ttgattgcat ccgtgttacc ggagctacca aggttgtttt
     1741 caaccacctt tacgatccag tctcccttgt tcgtgatcac accgttaagg agaagcttgt
     1801 tgagcgtgga atctctgttc aatcctacaa cggagatctt ctttacgaac catgggaaat
     1861 ttactgtgaa aagggaaagc cattcacttc tttcaattct tactggaaga agtgccttga
     1921 tatgtctatc gagtccgtta tgcttccacc accatggcgc cttatgccaa tcaccgctgc
     1981 tgctgaggct atctgggctt gctctatcga ggagcttgga cttgaaaacg aggctgagaa
     2041 gccatctaac gcccttctta cccgtgcttg gtctccagga tggtctaacg ctgacaagct
     2101 tcttaacgaa ttcatcgaaa agcaacttat cgattacgct aagaactcta agaaggtaag
     2161 tttaaacata tatatactaa ctaaccctga ttatttaaat tttcaggttg ttggaaactc
     2221 cacctccctt ctttctccat accttcactt cggagaaatc tccgttcgtc acgttttcca
     2281 atgcgctcgc atgaagcaga tcatctgggc tcgtgataag aactctgaag gagaggagtc
     2341 cgctgacctt ttccttcgcg gaatcggact tcgcgagtac tctcgctaca tttgtttcaa
     2401 cttcccattc actcatgagc aatcccttct ttctcacctt cgcttcttcc catgggatgc
     2461 tgatgtcgat aagttcaagg cttggcgcca aggacgcact ggatacccac ttgtcgatgc
     2521 tggaatgcgt gagctttggg ctactggatg gatgcataac cgcatccgtg ttatcgtttc
     2581 ctccttcgct gttaagttcc ttcttcttcc atggaaatgg ggaatgaaat atttctggga
     2641 cacccttctt gacgctgacc ttgagtgcga tatccttgga tggcagtaca tctctggatc
     2701 tatcccagat ggacacgagc ttgaccgtct tgacaaccca gctctccaag gagctaagta
     2761 cgatccagaa ggagaataca tccgccaatg gcttccagag cttgctcgcc ttccaactga
     2821 atggatccat cacccatggg atgccccact taccgtcctt aaggcttccg gagttgagct
     2881 tggaactaac tacgctaagc caatcgttga tatcgacacc gctcgtgagc ttcttgctaa
     2941 ggctatctct cgcacccgcg gagctcagat catgatcgga gccgctggtg gcggatctgg
     3001 gggtggatcc ggaggtacgc gaagagctta ctcgaattcg taatcatgtc atagctgttt
     3061 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     3121 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     3181 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     3241 gggagaggcg gtttgcgtat tggcgaactt ttgctgagtt gaaggatcag atcacgcatc
     3301 ttcccgacaa cgcagaccgt tccgtggcaa agcaaaagtt caaaatcagt aaccgtcagt
     3361 gccgataagt tcaaagttaa acctggtgtt gataccaaca ttgaaacgtt gatcgaaaac
     3421 gcgctgaaaa acgctgctga atgtgcgagc ttcttccgct tcctcgctca ctgactcgct
     3481 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     3541 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     3601 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     3661 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     3721 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     3781 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     3841 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     3901 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     3961 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     4021 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     4081 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     4141 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     4201 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     4261 gtggaactcc gtcga
//
