LOCUS       sequence-342523-        1689 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  The plasmid is part of a GG toolkit for cell-cell communication in 
            S. cerevisiae. This plasmid is an L0 part plasmid and contains the 
            Bar1 gene of Kazachstania naganishii (barrier part)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Krink N, Loechner AC, Anders A, Kahnt J, Rensing S, Hochberg G, 
            Sourjik V
  TITLE     Construction of multicellular yeast networks using the communication
            toolkit with variable specificity and attenuation
  JOURNAL   bioRxiv 2021
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021"
COMMENT     Sequence Label: pYCTK033
FEATURES             Location/Qualifiers
     source          1..1689
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgttgttgt tgtacgtcca agttttgctg gctgccattt ccccattctt gtcaattttg
       61 gttgaatgtt ccccattggc tttgccatcc actcatcaaa agttctccac ccagttggca
      121 aagagaaatc atttgaagtt tgatctccaa tttgaggatg aaagctttta tgcaacgaca
      181 ttaaagatcg gtgacccacc acaagagata accgttattt ttgattcagg ttctgctgac
      241 acatgggtta tgtctagata taatccattt tgtttgtcca gctctaacat tacggcaaat
      301 ggactaaaaa ctctctatga tggcaaggcc attattccca aagttgattg ttctcaaatt
      361 aaaacctaca atgctaacag gtctacgtct tttcaagaat tgaataatgg gcgtttttac
      421 attatttatg gagataaaac atttgctgat ggtatttggg gtaccgaaac gttcgaattt
      481 ggtggtgtga aaattcctaa cgttcaattt ggcctggcag attacgctac taccccaatt
      541 ggcggcgtgc tgggtatagg tttccaaaga agggaatcag tcaaaggtta tacaaatgca
      601 ccagaagtat tctatccaaa ttttccccag gtattgaaaa atgaaggtat catcgacact
      661 gttgcttatt ctctgtcttt gggtcaatcg tcacatatta tgtttggggc gatcgataga
      721 aactcataca tcggagatat ggttactttc ccaatgatta acatgttccc taatgctgtt
      781 agcaaaccag ctacccttag catcaccatc caggggctcg gtgctcagaa caagcaagag
      841 tgcaaatttc aaactttttc tacaaccaag taccctgctt tgctcgattc tgggtcgacg
      901 ctgatttctg ctccagtgga aatcgcagac aaaatggctg ctttcattgg ggcccaatgg
      961 tctgaaagcg acggaatata tactttagaa tgcccaaacg acacgaccaa tattgattat
     1021 attttcgatt ttggtgatat gaaattcaca ttgcctctaa gcaacttgat tcttccatcc
     1081 caaggcggca gtaatgtttg tggtttcggt ttgcaaagga gtgataatga gatcgtgttg
     1141 ggcgatgttt ttttatgttc gactttggta gtgtttgact tagatcatta tcaaatatct
     1201 ctcgccaaga gcaatcaaaa aagttcagca gaagatattg ttaatatacc aagtgacggt
     1261 aagattccag gtgccatcat tgctaaggct gaagcttgga ccacctatga accaataaca
     1321 gccacaaccg acatgtttga aactgaaaga tctcattgtg gcatcagaag tgctacctct
     1381 tctgttgtta actcagctaa aagctctttc actaatgtca agtctgcaac ttgggaagtt
     1441 gcctctaata caacaatcaa atcttccgtt gagtcatcgt caaaattatt tttgagcaag
     1501 ggattttcat caatgtcaaa ctccaaaaag acaggaattt cgacacatcc tcaacatgtt
     1561 aagagcttga gagcttctag tagtattatc tcaacaaaca cttcgcccgt cgaaatgagt
     1621 aaaataacta ttactactac gattagagaa acctctgtaa ttacaagaac agtttgcggt
     1681 actcaacca
//
