LOCUS       sequence-342525-        1761 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  The plasmid is part of a GG toolkit for cell-cell communication in 
            S. cerevisiae. This plasmid is an L0 part plasmid and contains the 
            Bar1 gene of Saccharomyces cerevisiae (barrier part)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Krink N, Loechner AC, Anders A, Kahnt J, Rensing S, Hochberg G, 
            Sourjik V
  TITLE     Construction of multicellular yeast networks using the communication
            toolkit with variable specificity and attenuation
  JOURNAL   bioRxiv 2021
REFERENCE   2  (bases 1 to 1761)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021"
COMMENT     Sequence Label: pYCTK034
FEATURES             Location/Qualifiers
     source          1..1761
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgtctgcaa ttaatcatct ttgtttgaaa cttattttgg cgagtttcgc gattattaac
       61 accattactg ctttaacaaa cgatggcact ggtcacttag aattcctttt acaacacgaa
      121 gaggagatgt attacgcaac aaccttagat ataggtacac cgtcccaaag tctgacagtg
      181 ttgtttgata ccggatctgc cgatttttgg gttatggatt ctagcaatcc cttctgctta
      241 ccaaattcaa atacgtcatc ctattcaaac gcaacttata atggcgaaga agttaagcct
      301 tcaattgatt gcaggtctat gagtacttat aatgagcata gatcttccac ctaccaatat
      361 ctggaaaatg gtaggtttta catcacatat gctgacggaa catttgctga cggtagttgg
      421 gggacggaaa ctgtatcaat taatggaatt gacatcccca atatccagtt cggagttgcc
      481 aagtatgcta cgacacccgt tagtggtgtt cttggaattg ggtttcctag aagagagtcc
      541 gttaagggct atgaaggtgc tcctaatgaa tattatccta attttcctca gattttaaaa
      601 agtgaaaaaa taatcgatgt ggtcgcgtat tcgctgttct taaactcacc tgattcaggt
      661 actggttcga ttgtttttgg tgccattgat gaatcaaagt tttctggtga tttgttcact
      721 ttccctatgg taaatgaata tcccacaata gtcgacgctc ctgcaacttt agcaatgact
      781 atacaaggat taggtgccca aaacaaaagt agttgtgaac atgaaacgtt tacgacgacc
      841 aagtatccag ttttgttgga ctcaggaacc tcgctattga atgcgcccaa ggtcatagca
      901 gataaaatgg cttcttttgt aaatgcgtcc tatagtgaag aggaaggtat atatatatta
      961 gactgtccag tatctgtagg tgacgtggaa tacaattttg atttcggcga tttgcaaata
     1021 agtgttccac tgtctagttt gattttaagt cccgagacag aaggcagcta ttgtgggttt
     1081 gcggtccagc caacaaacga ttcgatggtt ctgggtgatg tgttcctgtc ctctgcatac
     1141 gtcgtattcg atctcgataa ttataagata tctttagcac aggcaaattg gaacgcaagc
     1201 gaagtttcga aaaagctagt aaatattcaa acagatgggt ctatttcagg tgccaaaatt
     1261 gctacagctg aaccctggtc caccaatgaa ccatttacag tcacctctga catttattca
     1321 tctacaggct gcaagagtag gccttttctt caatcatcga cagcctcttc gcttattgca
     1381 gaaaccaacg tacaaagtcg caactgctct acgaagatgc caggcactag atcaactact
     1441 gtcttaagta agcctactca aaatagtgct atgcatcaaa gtacaggcgc tgtcacacaa
     1501 acctcaaatg aaactaaatt agaattatcc tcgactatgg caaattcggg cagtgtctcg
     1561 cttcccactt cgaattcaat agacaaagag ttcgaacatt cgaaatctca aactaccagc
     1621 gatccaagtg tagcagagca ttctacgttt aaccaaacgt ttgtacatga aactaaatat
     1681 cggcctactc ataagacagt cataacagaa actgtcacga agtattctac agtcttaata
     1741 aatgtctgta aaccaacata t
//
