LOCUS       sequence-342583-        1500 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes codon optimized putative thermophilic PET hydrolase enzyme 
            for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Erickson E, Gado JE, Avilan L, Bratti F, Brizendine RK, Cox PA, Gill
            R, Graham R, Kim DJ, Konig G, Michener WE, Poudel S, Ramirez KJ, 
            Shakespeare TJ, Zahn M, Boyd ES, Payne CM, DuBois JL, Pickford AR, 
            Beckham GT, McGeehan JE
  TITLE     Sourcing thermotolerant poly(ethylene terephthalate) hydrolase 
            scaffolds from natural diversity.
  JOURNAL   Nat Commun. 2022 Dec 21;13(1):7850. doi: 10.1038/s41467-022-35237-x.
  PUBMED    36543766
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-21"; volume: "13(1):7850. doi"; pages: " 
            10.1038/s41467-022-35237-x"
COMMENT     Sequence Label: pEE007
FEATURES             Location/Qualifiers
     source          1..1500
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 catatgcaag gcatcactgt gcgcaccccg ctgggtccgg cgttaggtca aatggaaaaa
       61 ggtgcgatcg cgttctacgg tctgccgtat gctcaggcgt ctcgtttcga agctccacgc
      121 ccagtagcgg cttggccgcc aggcgtaggc cgtgaacgtg ttgcgtgccc gcagactccg
      181 ggtactaccg cgcgtctggg tggctatatc ccgccgcaac gtgaagactg tctggtcgca
      241 aatctgttcc tgccgctgga accgccgcca cctgagggtt tcccggtaat ggtgtatctg
      301 catggcggcg gtttcacgtc tggctccgca gcagaaccaa tttacggtgg acaccgtatg
      361 gctcaggaag gtgtggttgt tgtttctgtt aactaccgcc tgggcccgct gggcttcctg
      421 gctcttccgg ccctggaaaa agaaaacccg aaggcagtag gtaactacgg tctgctggac
      481 ctggtagagg ctttacgttt cgtccagcgt cacatccgtt acttcggggg taacccgcag
      541 aacgttactc tgttcggtga atctgctggt ggcatgctgg tctgcaccct tctggctacc
      601 ccggaagcgc agggtctgtt ccacaaagcg attctgcaat ccggcggctg ccaccaggtc
      661 cgcccgctgg aacgcgattt ccctttcggg gaacagtggg ctaaaaacct ggggtgctct
      721 ccggaggact tggcttgcct gcgcaacctg ccactgtctc gcctgttccc aactatggaa
      781 ccgaaagcgc cgccggatat cacagctagc gctttgggct tcccaaactc cccgttcaaa
      841 ccgcacctgg gcgcactgct gccggaatcc ccgaccgagg cactgcgtaa gggccaagct
      901 cgcgatattc cgttgctcgt cggtgctaat ctcgaagagc tggctttccc gggcctggct
      961 tggctgctgg gtccgcgccg ttgggaagaa ttcggtcagc gcctggcggc gcaaggtctt
     1021 actcagcagc agcgtgaggc attaaaaggc gtttatcaga aacgtttctc cgagccgcgt
     1081 gccgcctggg gtcaagcgca gactgatctg cttctgctgt gcccatccct gaaagcagca
     1141 cgtctgcagg cctccttcgc cccgacctac gcgtatcttt tcacttttcg tgtgccaggt
     1201 ttcgaaggcc tcggtgcgtt ccacggtctg gaactggcgc cgctgtttgg caactttgaa
     1261 gaaatgccgt tcctgccgct gttcctgagc gctgaagctc gcgaaaaagc ggaagctctc
     1321 ggcaaacgta tgcgtcgtta ctgggttagc ttcgcacgcg aaggtgaacc gcgctcttgg
     1381 ccgcactggc ctacttatga agagggttac ttgctgcgcc tcgatgaacc gccgggtctg
     1441 atcccggatc tgtatgaaga acgttgcggt gtcctggaag cgctgggctt gctgctcgag
//
