LOCUS       sequence-342595-         597 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes codon optimized putative thermophilic PET hydrolase enzyme 
            for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Erickson E, Gado JE, Avilan L, Bratti F, Brizendine RK, Cox PA, Gill
            R, Graham R, Kim DJ, Konig G, Michener WE, Poudel S, Ramirez KJ, 
            Shakespeare TJ, Zahn M, Boyd ES, Payne CM, DuBois JL, Pickford AR, 
            Beckham GT, McGeehan JE
  TITLE     Sourcing thermotolerant poly(ethylene terephthalate) hydrolase 
            scaffolds from natural diversity.
  JOURNAL   Nat Commun. 2022 Dec 21;13(1):7850. doi: 10.1038/s41467-022-35237-x.
  PUBMED    36543766
REFERENCE   2  (bases 1 to 597)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-21"; volume: "13(1):7850. doi"; pages: " 
            10.1038/s41467-022-35237-x"
COMMENT     Sequence Label: pEE013
FEATURES             Location/Qualifiers
     source          1..597
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 catatgcgcg tactgtgctc ggctggttat gcggtactgc gcttcgacta tcgttgccac
       61 ggcgactctc cgctgccgtt cgaagaattc cgtattagca tggcggtaga agacgcggaa
      121 aacgcagtaa aatacgtaaa atcccttgaa cgtgtggacg gctcatcctt tgccgtcatt
      181 ggtctgtcta tgggcggtgg cgttgcagtc aaattggctg cgggtcgtga cgacgtggcg
      241 gcactggtac tgctttctcc ggccctggat tggccggaac tgaccggccg tgttccgttt
      301 aaagttgaag aaggttacgt ttacatgggt ccgttccgta tgcgtgcaga aaacgcgatg
      361 gaaaacgccc gtttcactgt tatggatatt gcagaacaag tgaaagctcc gacgctgatc
      421 gttcatgcta ccgatgacga agttgtacct atctctcagg ctaagcgttt ctatgagaaa
      481 cttcgtgtag aaaaacgttt cctggaagtt aagtccggtc atgttttcaa cgattaccac
      541 gttcgtcgta acctggaagg tgagatcctg tcgtgggtta aaagccacct gctcgag
//
