LOCUS       sequence-342611-         831 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes codon optimized putative thermophilic PET hydrolase enzyme 
            for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Erickson E, Gado JE, Avilan L, Bratti F, Brizendine RK, Cox PA, Gill
            R, Graham R, Kim DJ, Konig G, Michener WE, Poudel S, Ramirez KJ, 
            Shakespeare TJ, Zahn M, Boyd ES, Payne CM, DuBois JL, Pickford AR, 
            Beckham GT, McGeehan JE
  TITLE     Sourcing thermotolerant poly(ethylene terephthalate) hydrolase 
            scaffolds from natural diversity.
  JOURNAL   Nat Commun. 2022 Dec 21;13(1):7850. doi: 10.1038/s41467-022-35237-x.
  PUBMED    36543766
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-21"; volume: "13(1):7850. doi"; pages: " 
            10.1038/s41467-022-35237-x"
COMMENT     Sequence Label: pEE021
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 catatgagcg aactggagct gaacgaagtg ttcaaactga ttaaactggt ctcgttcatg
       61 aacaagggtc aacaaatcat tggtgtgctg cacaaaccgg ataaaatcaa accgcacgaa
      121 aaagtaccgg ggatcgttat gttccacggt tttactggta acaaatctga agctcaccgt
      181 ctgttcgtgc acatcgcacg tggtctgtct tcccgtggct tcatggtact gcgttttgat
      241 ttccgtggta gcggcgattc tgatggcgac ttcgaagata tgacactgcc ggaagaagtt
      301 tctgacgcgg agcgtgccat caccttcgta ctgcgtcagc gtaacgtcga ccgtgaaaaa
      361 atcggggtca ttggtctgtc cattggtggc cgtgtagcgg ccatcctggc gtctcgcgac
      421 gaacgtatta aattcgcagt gctgtactcc ccggcgctgg gccgtctgaa agaacgcttc
      481 ctgtccctca tgggcgaaga ggccctgcgt cggctgaact gtggcgaacc gatcgaagta
      541 agctctggct ggtacctgaa gaaagccttc ttcgaaaccg tggactacat cgtaccggtt
      601 gaagttatgt ccaacattcg cgttccggtt ctgatcattc acggtgatcg tgatgaaatc
      661 atccctgttg aagagagcat gaaagcatac gagcgcataa aaggtctgaa cgaaaaaaac
      721 gaactgtaca tcgttaaagg cggtgaccat accttctcta agcgcgaaca cactctggaa
      781 gtgctgaata aaacgatcga atggctgagc tctctgaacc tgatgctcga g
//
