LOCUS       sequence-342637-         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes codon optimized putative thermophilic PET hydrolase enzyme 
            for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Erickson E, Gado JE, Avilan L, Bratti F, Brizendine RK, Cox PA, Gill
            R, Graham R, Kim DJ, Konig G, Michener WE, Poudel S, Ramirez KJ, 
            Shakespeare TJ, Zahn M, Boyd ES, Payne CM, DuBois JL, Pickford AR, 
            Beckham GT, McGeehan JE
  TITLE     Sourcing thermotolerant poly(ethylene terephthalate) hydrolase 
            scaffolds from natural diversity.
  JOURNAL   Nat Commun. 2022 Dec 21;13(1):7850. doi: 10.1038/s41467-022-35237-x.
  PUBMED    36543766
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-21"; volume: "13(1):7850. doi"; pages: " 
            10.1038/s41467-022-35237-x"
COMMENT     Sequence Label: pEE034
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 catatgacta ccccaacgcc gacacctgaa ccggaaccgg aaccgccggg cggttgcggt
       61 gactgttatc agcgtgggcc tgacccgacc gtagcggcgc tggaagctga ccgcggtccg
      121 tattcagtcc gcaccattaa cgtttcaagc tgggtctctg gtttcggtgg tggaactatc
      181 cactacccgg taggtacaca gggcaccatg ggcgctatcg ctgtgatccc gggttacgtt
      241 tcttatgaaa actcgatcga atggtggggc ggccgtcttg cgtcatgggg cttcgttgta
      301 attacgatcg acactaactc catctacgat cagccggact cccgcgccaa ccagctgtct
      361 gctgctctgg attatgtgat cgcgcagagc aactccagcc gttctgcgat ccagggcatg
      421 gttgatccga accgcctggg tgcaatcggc tggtccatgg gtggcggcgg tactctgaaa
      481 ctgtctacgg accgttatct gaaggctgct attccgcagg cgccatggta ctccggcttt
      541 aacccgttcg atgaaatcac aacccctacc ctcatcatcg cttgccagct ggatgctgtc
      601 gccccagtgg cgcaacacgc tagtccgttc tacaacgaaa ttccgaactc taccgcaaaa
      661 gctttcctgg agatccgtaa cggtgaccac ttctgcgcaa acagcggtta cccggatgag
      721 gacatcctgg gtaaatatgg agttgcatgg atgaaacgtt tcatcgatga cgaccgtcgt
      781 tatgatgcat tcctgtgcgg tccgaaccac gaagctgaat gggatatctc tgaataccgc
      841 gacacttgca attacctcga g
//
