LOCUS       sequence-342667-         789 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes codon optimized putative thermophilic PET hydrolase enzyme 
            for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Erickson E, Gado JE, Avilan L, Bratti F, Brizendine RK, Cox PA, Gill
            R, Graham R, Kim DJ, Konig G, Michener WE, Poudel S, Ramirez KJ, 
            Shakespeare TJ, Zahn M, Boyd ES, Payne CM, DuBois JL, Pickford AR, 
            Beckham GT, McGeehan JE
  TITLE     Sourcing thermotolerant poly(ethylene terephthalate) hydrolase 
            scaffolds from natural diversity.
  JOURNAL   Nat Commun. 2022 Dec 21;13(1):7850. doi: 10.1038/s41467-022-35237-x.
  PUBMED    36543766
REFERENCE   2  (bases 1 to 789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-21"; volume: "13(1):7850. doi"; pages: " 
            10.1038/s41467-022-35237-x"
COMMENT     Sequence Label: pEE049
FEATURES             Location/Qualifiers
     source          1..789
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 catatggtgg aaatcggccc agcaccgacc tccacctcgc tgaacagtga cggctccttc
       61 gctgtgtcat ctgcgtctgt gagctcgtct gcatgcggct ccggttgcgc tggtggtact
      121 gtatactacc cgaacactgc tggcagctac ggtgtgatcg cggtttgtcc gggcttcacc
      181 aatacctcca gtgccatctc ctggtttgca cgtcgcatgg ctactcacgg tttcgtcact
      241 atcgcaatga acaccaactc ccgttatgac tttccagcaa gtcgcgctac ccagctgcgt
      301 gcggttctga attacctggt gaacagctct agcagcacga ttcgttcccg tatccgctcc
      361 gcagatcgtg gtgtgtctgg ttattccatg ggcggtggcg gtaccctgtt ggctagccgt
      421 gacgacagca ccttgaagac cggtgtcccg atggcgccgt ataattcagg tactatcagc
      481 ggcgtgaacg taccacagat gattatcggc ggttccaacg atagtatcgc gccggtttcc
      541 tctatggctc gtccattcta caacaacatc ccgtctaccg tgaaaaaagc actggcggtt
      601 ctgaatggcg catctcacct gacgttcacc tcctatgacg aacgtgcggc gcgctacggt
      661 gttgcgttcg cgaaacgctt cgctgacggt gacacccgtt atactccatt cctgtgtggc
      721 gctgaacaca cagcttacgc aacttcatct cgtttcacgg aatacagtag caattgcccg
      781 tatctcgag
//
