LOCUS       sequence-342673-         798 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes codon optimized putative thermophilic PET hydrolase enzyme 
            for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Erickson E, Gado JE, Avilan L, Bratti F, Brizendine RK, Cox PA, Gill
            R, Graham R, Kim DJ, Konig G, Michener WE, Poudel S, Ramirez KJ, 
            Shakespeare TJ, Zahn M, Boyd ES, Payne CM, DuBois JL, Pickford AR, 
            Beckham GT, McGeehan JE
  TITLE     Sourcing thermotolerant poly(ethylene terephthalate) hydrolase 
            scaffolds from natural diversity.
  JOURNAL   Nat Commun. 2022 Dec 21;13(1):7850. doi: 10.1038/s41467-022-35237-x.
  PUBMED    36543766
REFERENCE   2  (bases 1 to 798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-21"; volume: "13(1):7850. doi"; pages: " 
            10.1038/s41467-022-35237-x"
COMMENT     Sequence Label: pEE052
FEATURES             Location/Qualifiers
     source          1..798
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(589..600)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 catatggcac agaacccgta tgaacgtggc cctgcgccga ccgagcagag tgttcgtgcg
       61 gagcgtggcc cgtttgctat ttcccaggtg agcgtttctc gtctggcagt tagcggcttc
      121 ggcggcggca cgatctacta tccaacctcg actgcagaag gcacctttgg cgcagtggct
      181 atcgcaccgg gctacacggc ttctcagagc tctatggcgt ggtatggtcc acgtctggcg
      241 tctcagggtt ttgttatctt cactattgat accatcacga ccggtgacca gccggattcc
      301 cgtggtcgcc aactgctggc cgcgctggac tacctgaccc agcgtagttc cgtgcgctcc
      361 cgggttgatg cgagccgtct gggcgtcatg ggtcactcta tgggcggcgg tggtagcctt
      421 gaagccactg tctctcgtcc gtcattacaa gctgcgatcc ctctgacccc gtggaatctg
      481 gacaaaacct ggccggaagt ccgcgtaccg acgctgatca tcggtgcgga aaacgactct
      541 atcgcaccag ttagttctca cagcgaaccg ttctatgcgt ctctgccgtc gaccctcgat
      601 aaagcttatc tggaactgaa cggtgctagc cacttcgcac cgaatgttag cgacactacc
      661 attgcgcgct tttctatctc ctggctgaaa cgtttcatcg acaacgacac tcgttatgaa
      721 cagttcttat gcccacctcc gcgtgtatct accgagattt ccgaatatcg cgacacttgc
      781 ccgcactcag gtctcgag
//
