LOCUS       sequence-343043-        9763 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression vector encoding TRAPPC10 and EmGFP-Bsd.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9763)
  AUTHORS   Moyer AJ, Fernandez FX, Li Y, Klinedinst DK, Florea LD, Kazuki Y, 
            Oshimura M, Reeves RH
  TITLE     Overexpression screen of chromosome 21 genes reveals modulators of 
            Sonic hedgehog signaling relevant to Down syndrome.
  JOURNAL   Dis Model Mech. 2023 Apr 1;16(4):dmm049712. doi: 10.1242/dmm.049712.
            Epub 2023 Apr 13.
  PUBMED    36995257
REFERENCE   2  (bases 1 to 9763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dis Model 
            Mech."; date: "2023-04-1"; volume: "16(4):dmm049712. doi"; pages: " 
            10.1242/dmm.049712. Epub 2023 Apr 13"
COMMENT     Sequence Label: TRAPPC10_pcDNA6.2/EmGFP-Bsd
FEATURES             Location/Qualifiers
     source          1..9763
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    911..935
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4719..4743)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4769..4810
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(4850..4869)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4894..4942
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5144..5572
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5231..5250)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5441..5462
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5587..6086
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5622..5645)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6019..6038
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     promoter        6100..6147
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             join(6166..6168,6169..6171,6172..6882)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6211..6232)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6472..6491)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6819..6840
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             6883..7278
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="AKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    7436..7557
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7473..7492)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7527..7546
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7606..7622)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7606..7622)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7619..7641)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7630..7646
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7654..7660,7661..7678,7679..7684))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7699..7720
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7837..7854)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8008..8596)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8088..8107)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8767..9558,9559..9627))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPKLNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9390..9409
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9628..9732)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt
      901 taagctatca acaagtttgt acaaaaaagc aggcttaatg gacgcctctg aggagccgct
      961 gccgccggtg atctacacca tggagaacaa gcccatcgtc acctgtgctg gagatcagaa
     1021 tttatttacc tctgtttatc caacgctctc tcagcagctt ccaagagaac caatggaatg
     1081 gagaaggtcc tatggccggg ctccgaagat gattcaccta gagtctaact ttgttcaatt
     1141 caaagaggag ctgctgccca aagaaggaaa caaagctctg ctcacgtttc ccttcctcca
     1201 tatttactgg acagagtgct gtgataccga agtgtataaa gctacagtaa aagatgacct
     1261 caccaagtgg cagaatgttc tgaaggctca tagctctgtg gactggttaa tagtgatagt
     1321 tgaaaatgat gccaagaaaa aaaacaaaac caacatcctt ccccgaacct ctattgtgga
     1381 caaaataaga aatgattttt gtaataaaca gagtgacagg tgtgttgtgc tctccgaccc
     1441 cttgaaggac tcttctcgaa ctcaggaatc ctggaatgcc ttcctgacca aactcaggac
     1501 attgcttctt atgtctttta ccaaaaacct aggcaagttt gaggatgaca tgagaacctt
     1561 gagggagaag aggactgagc caggctggag cttttgtgaa tatttcatgg ttcaggagga
     1621 gcttgccttt gttttcgaga tgctgcagca gttcgaggac gccctggtgc agtacgacga
     1681 actggacgcc ctcttctctc agtatgtggt caacttcggg gccggggatg gtgccaactg
     1741 gctgactttt ttctgccagc cagtgaagag ctggaacgga ttgatcctcc gaaaacccat
     1801 agatatggag aagcgggaat cgatccagag gcgagaagcc accctgttag atctgcgcag
     1861 ttacctgttc tctcgccagt gcaccttgct gctcttcctg cagaggccgt gggaggtggc
     1921 ccagcgcgcc ctagagctgc tgcacaactg cgtgcaggaa ctgaagctct tagaagtctc
     1981 tgtcccacct ggtgctctgg actgctgggt gtttctgagc tgtctggagg tgttgcagag
     2041 gatagaaggc tgctgtgacc gggcacagat cgactcaaac attgcccaca ctgtggggct
     2101 atggagctat gccacagaaa agttaaagtc cttgggctat ctatgtggac ttgtgtcaga
     2161 gaaaggacct aactcagaag atctcaacag gacagttgac cttttggcag gtttgggagc
     2221 tgagcgacca gaaacagcca acacagctca gagtccttat aagaaactga aagaagcatt
     2281 atcgtcagtg gaagcttttg aaaaacacta cttagatttg tcccatgcca ccattgaaat
     2341 gtatacaagc attgggagga ttcgatctgc taagtttgtt ggaaaagatc tggcagagtt
     2401 ttacatgagg aaaaaggctc cacaaaaggc agaaatctat cttcaaggag cactgaaaaa
     2461 ctacctggct gagggctggg cactccccat cacacacaca aggaagcagc tggccgaatg
     2521 tcaaaagcac cttggacaaa ttgaaaacta cctgcagacc agcagcctct tagccagtga
     2581 ccaccacctc actgaagagg agcgcaagca cttctgccag gagatacttg actttgccag
     2641 ccagccgtca gacagcccag gtcataagat agtgctaccc atgcattcct ttgcacaact
     2701 gcgagatctc cattttgatc cctccaatgc cgtggtccac gtgggcggcg ttttgtgcgt
     2761 tgagataacc atgtacagcc agatgcctgt gcctgttcac gtggagcaga ttgtggtcaa
     2821 tgtccacttc agcattgaga aaaacagcta ccggaagact gcggagtggc ttaccaagca
     2881 caagacgtcc aatgggatca ttaactttcc acccgagacc gcacctttcc ctgtatccca
     2941 aaacagtttg cccgcgctgg agttgtatga aatgtttgag agaagcccat ctgataactc
     3001 cttgaacacg actgggatta tctgcagaaa cgtccacatg ctcctgagaa ggcaggagag
     3061 cagctcctct ctagagatgc cctcaggggt ggctctggag gagggtgccc acgtgctgag
     3121 gtgcagccac gtgaccctgg aaccaggggc caaccagata acattcagga ctcaggccaa
     3181 ggaacctgga acgtatacac tcaggcagct gtgcgcctcg gtgggctccg tgtggttcgt
     3241 cctccctcac atctacccca ttgtgcagta cgacgtgtac tcacaggagc cccagctgca
     3301 cgtggagccg ctggctgata gccttctggc aggcattcct cagagagtca agttcactgt
     3361 cactaccggc cattatacga taaagaatgg agacagcctg cagcttagca atgccgaagc
     3421 catgctcatc ctgtgccagg cggagagcag ggctgtggtc tactccaaca cgagagaaca
     3481 gtcttctgag gccgcgctcc ggattcagtc ctccgacaag gtcacgagca tcagtctgcc
     3541 tgttgcgcct gcgtaccacg tgatcgaatt tgaactggaa gttctctctt taccttcagc
     3601 cccagcactc ggaggggaga gtgacatgct ggggatggca gagccccaca ggaagcataa
     3661 ggacaaacag agaactggcc gctgcatggt taccacagac cacaaagtgt cgattgactg
     3721 cccgtggtcc atctactcca cagtcatcgc actgaccttc agcgtaccct tcaggaccac
     3781 acacagcctc ctgtcctcag gaacacggaa atatgttcaa gtttgtgtcc agaatttgtc
     3841 agaacttgac tttcagctgt cagatagtta tcttgtagat accggtgata gtaccgacct
     3901 gcaactagta ccactgaaca cgcagtccca gcagcccatc tacagcaagc agtcggtgtt
     3961 cttcgtctgg gaactcaagt ggacagaaga gcctccccct tctctgcatt gccggttctc
     4021 tgttggattt tccccagctt ctgaggaaca gctgtctatc tccttaaagc cgtatactta
     4081 tgaatttaaa gtggaaaatt tttttacatt atacaacgtg aaggctgaga tctttccccc
     4141 ttcgggaatg gagtattgca gaacaggctc cctctgctcc ctggaggttt tgatcacgag
     4201 gctctcagac ctcttggagg tggataaaga tgaagcactg actgaatctg atgagcattt
     4261 ttcgacaaag cttatgtatg aagttgtcga caacagtagc aactgggcag tgtgtgggaa
     4321 aagctgcggt gtcatctcca tgccagtggc tgctcgggcc actcacaggg tccacatgga
     4381 agtgatgccg ctcttcgccg ggtatctccc cctgcccgac gtcaggctgt tcaagtacct
     4441 cccccatcat tctgcacact cctcccaact ggacgctgac agctggatag aaaacgacag
     4501 cctgtcagta gacaagcacg gggacgacca gccggacagc agcagcctca agagcagggg
     4561 cagcgtgcat tcggcctgca gcagcgagca caaaggccta cccatgcccc ggctgcaggc
     4621 actgccggcc ggccaggtct tcaactccag ctcgggcaca caagtcctgg tcatccccag
     4681 ccaagatgac cacgtcctgg aagtcagtgt aacatagtac ccagctttct tgtacaaagt
     4741 ggttgatcta gagggcccgc ggttcgaagg taagcctatc cctaaccctc tcctcggtct
     4801 cgattctacg cgtaccggtt agtaatgagt ttaaacgggg gaggctaact gaaacacgga
     4861 aggagacaat accggaagga acccgcgcta tgacggcaat aaaaagacag aataaaacgc
     4921 acgggtgttg ggtcgtttgt tcataaacgc ggggttcggt cccagggctg gcactctgtc
     4981 gataccccac cgagacccca ttggggccaa tacgcccgcg tttcttcctt ttccccaccc
     5041 caccccccaa gttcgggtga aggcccaggg ctcgcagcca acgtcggggc ggcaggccct
     5101 gccatagcag atctgcgcag ctggggctct agggggtatc cccacgcgcc ctgtagcggc
     5161 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     5221 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     5281 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     5341 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     5401 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     5461 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     5521 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttatcctcga
     5581 gctaccgggt aggggaggcg cttttcccaa ggcagtctgg agcatgcgct ttagcagccc
     5641 cgctgggcac ttggcgctac acaagtggcc tctggcctcg cacacattcc acatccaccg
     5701 gtaggcgcca accggctccg ttctttggtg gccccttcgc gccaccttct actcctcccc
     5761 tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg caggacgtga caaatggaag
     5821 tagcacgtct cactagtctc gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag
     5881 gcctttgggg cagcggccaa tagcagcttt gctccttcgc tttctgggct cagaggctgg
     5941 gaaggggtgg gtccgggggc gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa
     6001 ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct
     6061 cctcttcctc atctccgggc ctttcgactc tagacacgtg ttgacaatta atcatcggca
     6121 tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggt gagcaagggc
     6181 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     6241 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     6301 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccttc
     6361 acctacggcg tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc
     6421 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     6481 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     6541 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     6601 tacaacagcc acaaggtcta tatcaccgcc gacaagcaga agaacggcat caaggtgaac
     6661 ttcaagaccc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     6721 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
     6781 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     6841 accgccgccg ggatcactct cggcatggac gagctgtaca aggccaagcc tttgtctcaa
     6901 gaagaatcca ccctcattga aagagcaacg gctacaatca acagcatccc catctctgaa
     6961 gactacagcg tcgccagcgc agctctctct agcgacggcc gcatcttcac tggtgtcaat
     7021 gtatatcatt ttactggggg accttgtgca gaactcgtgg tgctgggcac tgctgctgct
     7081 gcggcagctg gcaacctgac ttgtatcgtc gcgatcggaa atgagaacag gggcatcttg
     7141 agcccctgcg gacggtgccg acaggtgctt ctcgatctgc atcctgggat caaagccata
     7201 gtgaaggaca gtgatggaca gccgacggca gttgggattc gtgaattgct gccctctggt
     7261 tatgtgtggg agggctaagc acttcgtggc cgaggagcag gactgacacg tgctacgaga
     7321 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
     7381 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt
     7441 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     7501 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     7561 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc
     7621 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     7681 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     7741 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     7801 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     7861 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     7921 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     7981 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     8041 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     8101 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     8161 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     8221 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     8281 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     8341 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     8401 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     8461 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     8521 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     8581 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     8641 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     8701 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     8761 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     8821 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     8881 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     8941 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     9001 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     9061 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     9121 ttcattcagc ttcggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     9181 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     9241 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     9301 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     9361 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     9421 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     9481 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     9541 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     9601 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     9661 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     9721 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtc
//
