LOCUS       sequence-343076-        2744 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GFP expression under the control of E. coli fxsA promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Saltepe B, Bozkurt EU, Haciosmanoglu N, Seker UOS
  TITLE     Genetic Circuits To Detect Nanomaterial Triggered Toxicity through 
            Engineered Heat Shock Response Mechanism.
  JOURNAL   ACS Synth Biol. 2019 Oct 18;8(10):2404-2417. doi: 
            10.1021/acssynbio.9b00291. Epub 2019 Oct 2.
  PUBMED    31536326
REFERENCE   2  (bases 1 to 2744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00291"; journalName: "ACS Synth Biol"; date: 
            "2019-10-18- 18"; volume: "8"; issue: "10"; pages: "2404-2417"
COMMENT     Sequence Label: PfxsA GFP pZa
FEATURES             Location/Qualifiers
     source          1..2744
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      13..99
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(134..151)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(268..813)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(348..367)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(927..1021)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1042..1701)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1616..1635
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(1702..1804)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             2023..2736
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(2053..2081)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2676..2696
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 tacgcgtggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
       61 tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgcccta gacctaggga
      121 tatattccgc ttcctcgctc actgactcgc tacgctcggt cgttcgactg cggcgagcgg
      181 aaatggctta cgaacggggc ggagatttcc tggaagatgc caggaagata cttaacaggg
      241 aagtgagagg gccgcggcaa agccgttttt ccataggctc cgcccccctg acaagcatca
      301 cgaaatctga cgctcaaatc agtggtggcg aaacccgaca ggactataaa gataccaggc
      361 gtttccccct ggcggctccc tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt
      421 cattccgctg ttatggccgc gtttgtctca ttccacgcct gacactcagt tccgggtagg
      481 cagttcgctc caagctggac tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct
      541 tatccggtaa ctatcgtctt gagtccaacc cggaaagaca tgcaaaagca ccactggcag
      601 cagccactgg taattgattt agaggagtta gtcttgaagt catgcgccgg ttaaggctaa
      661 actgaaagga caagttttgg tgactgcgct cctccaagcc agttacctcg gttcaaagag
      721 ttggtagctc agagaacctt cgaaaaaccg ccctgcaagg cggttttttc gttttcagag
      781 caagagatta cgcgcagacc aaaacgatct caagaagatc atcttattaa tcagataaaa
      841 tatttctaga tttcagtgca atttatctct tcaaatgtag cacctgaagt cagccccata
      901 cgatataagt tgttactagt gcttggattc tcaccaataa aaaacgcccg gcggcaaccg
      961 agcgttctga acaaatccag atggagttct gaggtcatta ctggatctat caacaggagt
     1021 ccaagcgagc tcgatatcaa attacgcccc gccctgccac tcatcgcagt actgttgtaa
     1081 ttcattaagc attctgccga catggaagcc atcacaaacg gcatgatgaa cctgaatcgc
     1141 cagcggcatc agcaccttgt cgccttgcgt ataatatttg cccatggtga aaacgggggc
     1201 gaagaagttg tccatattgg ccacgtttaa atcaaaactg gtgaaactca cccagggatt
     1261 ggctgagacg aaaaacatat tctcaataaa ccctttaggg aaataggcca ggttttcacc
     1321 gtaacacgcc acatcttgcg aatatatgtg tagaaactgc cggaaatcgt cgtggtattc
     1381 actccagagc gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac aagggtgaac
     1441 actatcccat atcaccagct caccgtcttt cattgccata cggaattccg gatgagcatt
     1501 catcaggcgg gcaagaatgt gaataaaggc cggataaaac ttgtgcttat ttttctttac
     1561 ggtctttaaa aaggccgtaa tatccagctg aacggtctgg ttataggtac attgagcaac
     1621 tgactgaaat gcctcaaaat gttctttacg atgccattgg gatatatcaa cggtggtata
     1681 tccagtgatt tttttctcca ttttagcttc cttagctcct gaaaatctcg ataactcaaa
     1741 aaatacgccc ggtagtgatc ttatttcatt atggtgaaag ttggaacctc ttacgtgccg
     1801 atcaacgtct cattttcgcc agatatcgac gtcctcgagg agctcgagga tttctaccgt
     1861 aatctggatc actttaagtg tcggttttta ccccttaatt attaatttgt gaaatagatc
     1921 accgctttgg gattactacc aaaaatagtt gcgcaaacat cttgaaattt tgctaatgac
     1981 cacaatataa gctaaagaaa gcacgacgga tcgggaggta ccatgcgtaa aggagaagaa
     2041 cttttcactg gagttgtccc aattcttgtt gaattagatg gtgatgttaa tgggcacaaa
     2101 ttttctgtca gtggagaggg tgaaggtgat gcaacatacg gaaaacttac ccttaaattt
     2161 atttgcacta ctggaaaact acctgttcca tggccaacac ttgtcactac tttcggttat
     2221 ggtgttcaat gctttgcgag atacccagat catatgaaac agcatgactt tttcaagagt
     2281 gccatgcccg aaggttatgt acaggaaaga actatatttt tcaaagatga cgggaactac
     2341 aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg ttaatagaat cgagttaaaa
     2401 ggtattgatt ttaaagaaga tggaaacatt cttggacaca aattggaata caactataac
     2461 tcacacaatg tatacatcat ggcagacaaa caaaagaatg gaatcaaagt taacttcaaa
     2521 attagacaca acattgaaga tggaagcgtt caactagcag accattatca acaaaatact
     2581 ccaattggcg atggccctgt ccttttacca gacaaccatt acctgtccac acaatctgcc
     2641 ctttcgaaag atcccaacga aaagagagac cacatggtcc ttcttgagtt tgtaacagct
     2701 gctgggatta cacatggcat ggatgaacta tacaaataaa agct
//
