LOCUS       sequence-343192-        4023 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  B.1.617.1 variant S-protein with c-terminal FLAG tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4023)
  TITLE     Spike variants with FLAG tags
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4023)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCAGGS-S-B.1.617.1-FLAG
FEATURES             Location/Qualifiers
     source          1..4023
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..45
                     /label=CMVd3 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields strongly reduced expression 
                     relative to the full-length promoter"
     primer_bind     42..66
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             complement(1484..1501)
                     /codon_start=1
                     /product="tetracysteine peptide that binds biarsenical 
                     labeling reagents"
                     /label=tetracysteine tag
                     /translation="CCPGCC"
     CDS             3997..4020
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctcgttt agtgaaccgt
       61 cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca ccgggaccga
      121 tccagcctcc ggactgagct ccttaaggtt aactctagag aattcggatc catgtttgtg
      181 ttcctggtgc tgctgccact ggtgtccagc cagtgtgtga acctgaccac caggacccaa
      241 cttcctcctg cctacaccaa ctccttcacc aggggagtct actaccctga caaggtgttc
      301 aggtcctctg tgctgcacag cacccaggac ctgttcctgc cattcttcag caatgtgacc
      361 tggttccatg ccatccatgt gtctggcacc aatggcacca agaggtttga caaccctgtg
      421 ctgccattca atgatggagt ctactttgcc agcacagaga agagcaacat catcaggggc
      481 tggatttttg gcaccaccct ggacagcaag acccagtccc tgctgattgt gaacaatgcc
      541 accaatgtgg tgattaaggt gtgtgagttc cagttctgta atgacccatt cctggacgtc
      601 tactaccaca agaacaacaa gtcctggatg aagtctgagt tcagggtcta ctcctctgcc
      661 aacaactgta cctttgaata tgtgagccaa ccattcctga tggacttgga gggcaagcag
      721 ggcaacttca agaacctgag ggagtttgtg ttcaagaaca ttgatggcta cttcaagatt
      781 tacagcaaac acacaccaat caacctggtg agggacctgc cacagggctt ctctgccttg
      841 gaaccactgg tggacctgcc aattggcatc aacatcacca ggttccagac cctgctggct
      901 ctgcacaggt cctacctgac acctggagac tcctcctctg gctggacagc aggagcagca
      961 gcctactatg tgggctacct ccaaccaagg accttcctgc tgaaatacaa tgagaatggc
     1021 accatcacag atgctgtgga ctgtgccctg gacccactgt ctgagaccaa gtgtaccctg
     1081 aaatccttca cagtggagaa gggcatctac cagaccagca acttcagggt ccaaccaaca
     1141 gagagcattg tgaggtttcc aaacatcacc aacctgtgtc catttggaga ggtgttcaat
     1201 gccaccaggt ttgcctctgt ctatgcctgg aacaggaaga ggattagcaa ctgtgtggct
     1261 gactactctg tgctctacaa ctctgcctcc ttcagcacct tcaagtgtta tggagtgagc
     1321 ccaaccaaac tgaatgacct gtgtttcacc aatgtctatg ctgactcctt tgtgattagg
     1381 ggagatgagg tgagacagat tgcccctgga caaacaggca agattgctga ctacaactac
     1441 aaactgcctg atgacttcac aggctgtgtg attgcctgga acagcaacaa cctggacagc
     1501 aaggtgggag gcaactacaa ctacaggtac agactgttca ggaagagcaa cctgaaacca
     1561 tttgagaggg acatcagcac agagatttac caggctggca gcacaccatg taatggagtg
     1621 cagggcttca actgttactt tccactccaa tcctatggct tccaaccaac caatggagtg
     1681 ggctaccaac catacagggt ggtggtgctg tcctttgaac tgctccatgc ccctgccaca
     1741 gtgtgtggac caaagaagag caccaacctg gtgaagaaca agtgtgtgaa cttcaacttc
     1801 aatggactga caggcacagg agtgctgaca gagagcaaca agaagttcct gccattccaa
     1861 cagtttggca gggacattgc tgacaccaca gatgctgtga gggacccaca gaccttggag
     1921 attctggaca tcacaccatg ttcctttgga ggagtgtctg tgattacacc tggcaccaac
     1981 accagcaacc aggtggctgt gctctaccag ggtgtgaact gtactgaggt gcctgtggct
     2041 atccatgctg accaacttac accaacctgg agggtctaca gcacaggcag caatgtgttc
     2101 cagaccaggg ctggctgtct gattggagca gagcatgtga acaactccta tgagtgtgac
     2161 atcccaattg gagcaggcat ctgtgcctcc taccagaccc agaccaacag cagaaggagg
     2221 gcaaggtctg tggcaagcca gagcatcatt gcctacacaa tgagtctggg agcagagaac
     2281 tctgtggctt acagcaacaa cagcattgcc atcccaacca acttcaccat ctctgtgacc
     2341 acagagattc tgcctgtgag tatgaccaag acctctgtgg actgtacaat gtatatctgt
     2401 ggagacagca cagagtgtag caacctgctg ctccaatatg gctccttctg tacccaactt
     2461 aacagggctc tgacaggcat tgctgtggaa caggacaaga acacccagga ggtgtttgcc
     2521 caggtgaagc agatttacaa gacacctcca atcaaggact ttggaggctt caacttcagc
     2581 cagattctgc ctgacccaag caagccaagc aagaggtcct tcattgagga cctgctgttc
     2641 aacaaggtga ccctggctga tgctggcttc atcaagcaat atggagactg tctgggagac
     2701 attgctgcca gggacctgat ttgtgcccag aagttcaatg gactgacagt gctgcctcca
     2761 ctgctgacag atgagatgat tgcccaatac acctctgccc tgctggctgg caccatcacc
     2821 tctggctgga cctttggagc aggagcagcc ctccaaatcc catttgctat gcagatggct
     2881 tacaggttca atggcattgg agtgacccag aatgtgctct atgagaacca gaaactgatt
     2941 gccaaccagt tcaactctgc cattggcaag attcaggact ccctgtccag cacagcctct
     3001 gccctgggca aactccaaga tgtggtgaac cagaatgccc aggctctgaa caccctggtg
     3061 aagcaacttt ccagcaactt tggagccatc tcctctgtgc tgaatgacat cctgagcaga
     3121 ctggacaagg tggaggctga ggtccagatt gacagactga ttacaggcag actccaatcc
     3181 ctccaaacct atgtgaccca acaacttatc agggctgctg agattagggc atctgccaac
     3241 ctggctgcca ccaagatgag tgagtgtgtg ctgggacaaa gcaagagggt ggacttctgt
     3301 ggcaagggct accacctgat gagttttcca cagtctgccc ctcatggagt ggtgttcctg
     3361 catgtgacct atgtgcctgc ccatgagaag aacttcacca cagcccctgc catctgccat
     3421 gatggcaagg ctcactttcc aagggaggga gtgtttgtga gcaatggcac cgactggttt
     3481 gtgacccaga ggaacttcta tgaaccacag attatcacca cagacaacac ctttgtgtct
     3541 ggcaactgtg atgtggtgat tggcattgtg aacaacacag tctatgaccc actccaacct
     3601 gaactggact ccttcaagga ggaactggac aaatacttca agaaccacac cagccctgat
     3661 gtggacctgg gagacatctc tggcatcaat gcctctgtgg tgaacatcca gaaggagatt
     3721 gacagactga atgaggtggc taagaacctg aatgagtccc tgattgacct ccaagaactg
     3781 ggcaaatatg aacaatacat caagtggcca tggtacatct ggctgggctt cattgctgga
     3841 ctgattgcca ttgtgatggt caccatcatg ctgtgctgca tgacctcatg ttgctcctgt
     3901 cttaaggggt gctgctcctg cggttcctgt tgcaaatttg acgaggacga cagcgaaccg
     3961 gtgcttaagg gtgtgaagct gcattacacc ggaggcgatt acaaggatga cgatgacaag
     4021 tga
//
