LOCUS       sequence-34336-e         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Sun Q, Westphal W, Wong KN, Tan I, Zhong Q
  TITLE     Rubicon controls endosome maturation as a Rab7 effector.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Nov 9. 107(45):19338-43.
  PUBMED    20974968
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2010 Nov 9. 107(45):19338-43."
COMMENT     Sequence Label: EGFP-Rab7A
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             32..58
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             59..85
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(744..762)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(745..762)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(782..799)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 cggcatggac gagctgtaca agggatccat gtacccatac gatgttccgg attacgctta
       61 cccatacgat gttccggatt acgctggtac ctctaggaag aaagtgttgc tgaaggttat
      121 catcctggga gattctggag tcgggaagac atcactcatg aaccagtatg tgaataagaa
      181 attcagcaat cagtacaaag ccacaatagg agctgacttt ctgaccaagg aggtgatggt
      241 ggatgacagg ctagtcacaa tgcagatatg ggacacagca ggacaggaac ggttccagtc
      301 tctcggtgtg gccttctaca gaggtgcaga ctgctgcgtt ctggtatttg atgtgactgc
      361 ccccaacaca ttcaaaaccc tagatagctg gagagatgag tttctcatcc aggccagtcc
      421 ccgagatcct gaaaacttcc catttgttgt gttgggaaac aagattgacc tcgaaaacag
      481 acaagtggcc acaaagcggg cacaggcctg gtgctacagc aaaaacaaca ttccctactt
      541 tgagaccagt gccaaggagg ccatcaacgt ggagcaggcg ttccagacga ttgcacggaa
      601 tgcacttaag caggaaacgg aggtggagct gtacaacgaa tttcctgaac ctatcaaact
      661 ggacaagaat gaccgggcca aggcctcggc agaaagctgc agttgctaag cggccgctcg
      721 agcatgcatc tagagggccc tattctatag tgtcacctaa atgctagagc tcgctgatca
      781 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
      841 ttgaccctgg aa
//
