LOCUS       sequence-343501-       10270 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of mCherry-HP1A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10270)
  AUTHORS   Gao Y, Han M, Shang S, Wang H, Qi LS
  TITLE     Interrogation of the dynamic properties of higher-order 
            heterochromatin using CRISPR-dCas9.
  JOURNAL   Mol Cell. 2021 Aug 13. pii: S1097-2765(21)00612-2. doi: 
            10.1016/j.molcel.2021.07.034.
  PUBMED    34428454
REFERENCE   2  (bases 1 to 10270)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2021 Aug 13. pii: S1097-2765(21)00612-2. doi: 
            10.1016/j.molcel.2021.07.034."
COMMENT     Sequence Label: pHR: pPGK mCherry-HP1A
FEATURES             Location/Qualifiers
     source          1..10270
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(84..100)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(85..101)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             191..424
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(449..467)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(450..467)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(757..776)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(990..1009)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1109..1131
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1169..1187)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1255..1359
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1360..1428,1429..2220)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1578..1597)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2391..2979
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2880..2899
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3133..3150
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(3220..3240)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3225..3554
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3405..3540
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          4688..4753
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4883..4903
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5328..5462
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5365..5384)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5419..5438
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5630..6263
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6310..6435
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6932..7165
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7350..7394
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7679..7796
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7861..8360
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7896..7919)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     8293..8312
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      8418..8427
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8424..9131
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(8569..8587)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     8837..8856
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    join(9763..10270,1..81)
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9816..9836)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10234..10245)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
       61 ccctttgggc cgcctccccg catcgatacc gtcgacctcg agggaattaa ttcgagctcg
      121 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
      181 aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt
      241 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
      301 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
      361 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
      421 agcagcatct agaattaatt ccgtgtattc tatagtgtca cctaaatcgt atgtgtatga
      481 tacataaggt tatgtattaa ttgtagccgc gttctaacga caatatgtac aagcctaatt
      541 gtgtagcatc tggcttactg aagcagaccc tatcatctct ctcgtaaact gccgtcagag
      601 tcggtttggt tggacgaacc ttctgagttt ctggtaacgc cgtcccgcac ccggaaatgg
      661 tcagcgaacc aatcagcagg gtcatcgcta gccagatcct ctacgccgga cgcatcgtgg
      721 ccggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg
      781 gggaagatcg ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg
      841 caggccccgt ggccggggga ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg
      901 cggcggtgct caacggcctc aacctactac tgggctgctt cctaatgcag gagtcgcata
      961 agggagagcg tcgaatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     1021 cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
     1081 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
     1141 tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     1201 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     1261 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     1321 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     1381 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     1441 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     1501 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     1561 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     1621 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     1681 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     1741 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     1801 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     1861 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     1921 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     1981 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     2041 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     2101 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     2161 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     2221 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     2281 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     2341 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     2401 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     2461 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     2521 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     2581 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     2641 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     2701 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     2761 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     2821 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     2881 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     2941 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     3001 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     3061 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     3121 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     3181 cctctccccg cgcgtcggcc gattcattaa tgcagctgtg gaatgtgtgt cagttagggt
     3241 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     3301 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     3361 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     3421 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     3481 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     3541 taggcttttg caaaaagctt ggacacaaga caggcttgcg agatatgttt gagaatacca
     3601 ctttatcccg cgtcagggag aggcagtgcg taaaaagacg cggactcatg tgaaatactg
     3661 gtttttagtg cgccagatct ctataatctc gcgcaaccta ttttcccctc gaacactttt
     3721 taagccgtag ataaacaggc tgggacactt cacatgagcg aaaaatacat cgtcacctgg
     3781 gacatgttgc agatccatgc acgtaaactc gcaagccgac tgatgccttc tgaacaatgg
     3841 aaaggcatta ttgccgtaag ccgtggcggt ctgtaccggg tgcgttactg gcgcgtgaac
     3901 tgggtattcg tcatgtcgat accgtttgta tttccagcta cgatcacgac aaccagcgcg
     3961 agcttaaagt gctgaaacgc gcagaaggcg atggcgaagg cttcatcgtt attgatgacc
     4021 tggtggatac cggtggtact gcggttgcga ttcgtgaaat gtatccaaaa gcgcactttg
     4081 tcaccatctt cgcaaaaccg gctggtcgtc cgctggttga tgactatgtt gttgatatcc
     4141 cgcaagatac ctggattgaa cagccgtggg atatgggcgt cgtattcgtc ccgccaatct
     4201 ccggtcgcta atcttttcaa cgcctggcac tgccgggcgt tgttcttttt aacttcaggc
     4261 gggttacaat agtttccagt aagtattctg gaggctgcat ccatgacaca ggcaaacctg
     4321 agcgaaaccc tgttcaaacc ccgctttaaa catcctgaaa cctcgacgct agtccgccgc
     4381 tttaatcacg gcgcacaacc gcctgtgcag tcggcccttg atggtaaaac catccctcac
     4441 tggtatcgca tgattaaccg tctgatgtgg atctggcgcg gcattgaccc acgcgaaatc
     4501 ctcgacgtcc aggcacgtat tgtgatgagc gatgccgaac gtaccgacga tgatttatac
     4561 gatacggtga ttggctaccg tggcggcaac tggatttatg agtgggcccc ggatctttgt
     4621 gaaggaacct tacttctgtg gtgtgacata attggacaaa ctacctacag agatttaaag
     4681 ctctaaggta aatataaaat ttttaagtgt ataatgtgtt aaactactga ttctaattgt
     4741 ttgtgtattt tagattccaa cctatggaac tgatgaatgg gagcagtggt ggaatgcctt
     4801 taatgaggaa aacctgtttt gctcagaaga aatgccatct agtgatgatg aggctactgc
     4861 tgactctcaa cattctactc ctccaaaaaa gaagagaaag gtagaagacc ccaaggactt
     4921 tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa ctcttgcttg
     4981 ctttgctatt tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa ttatggaaaa
     5041 atattctgta acctttataa gtaggcataa cagttataat cataacatac tgttttttct
     5101 tactccacac aggcatagag tgtctgctat taataactat gctcaaaaat tgtgtacctt
     5161 tagcttttta atttgtaaag gggttaataa ggaatatttg atgtatagtg ccttgactag
     5221 agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac
     5281 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
     5341 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     5401 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga
     5461 tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta
     5521 ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct gaattatttt
     5581 cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagtt ggaagggcta
     5641 attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac acaaggctac
     5701 ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga
     5761 tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag
     5821 aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagtg
     5881 ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg
     5941 gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc tggggacttt
     6001 ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc ctgcatataa
     6061 gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct
     6121 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     6181 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     6241 gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa
     6301 ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag
     6361 gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat
     6421 gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg
     6481 ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag
     6541 ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata
     6601 ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat
     6661 acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct
     6721 ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccggc
     6781 cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata
     6841 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag
     6901 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag
     6961 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat
     7021 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc
     7081 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa
     7141 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca
     7201 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc
     7261 acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct
     7321 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata
     7381 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat
     7441 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag
     7501 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga
     7561 ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat
     7621 ccattcgatt agtgaacgga tctcgacggt atcgccaaat ggcagtattc atccacaatt
     7681 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     7741 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg
     7801 tttattacag ggacagcaga gatccagttt ggatcgataa gcttgatatc gaattctacc
     7861 gggtagggga ggcgcttttc ccaaggcagt ctggagcatg cgctttagca gccccgctgg
     7921 gcacttggcg ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtaggc
     7981 gccaaccggc tccgttcttt ggtggcccct tcgcgccacc ttctactcct cccctagtca
     8041 ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac
     8101 gtctcactag tctcgtgcag atggacagca ccgctgagca atggaagcgg gtaggccttt
     8161 ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagagg ctgggaaggg
     8221 gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct
     8281 ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt
     8341 cctcatctcc gggcctttcg acctgcagcc caagcttacg cgtctgcagg atatcaagct
     8401 tgcggtacga ctgagtcgcc accatggtga gcaagggcga ggaggataac atggccatca
     8461 tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg
     8521 agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg
     8581 tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg
     8641 gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc
     8701 ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga
     8761 cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca
     8821 acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg
     8881 agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga
     8941 aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc
     9001 agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact
     9061 acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg
     9121 agctgtacaa gggtggaggt cggaccggat ccggaaagaa aaccaagcgg acagctgaca
     9181 gttcttcttc agaggatgag gaggagtatg tcgtggagaa ggtgctagac aggcgcgtgg
     9241 ttaagggaca agtggaatat ctactgaagt ggaaaggctt ttctgaggag cacaatactt
     9301 gggaacctga gaaaaacttg gattgccctg agctaatttc tgaatttatg aaaaagtata
     9361 agaagatgaa ggagggtgaa aataataaac ccagggagaa gtcagaaagt aacaagagga
     9421 aatccaattt ctcaaacagt gccgatgaca tcaaatctaa aaaaaagaga gagcagagca
     9481 atgatatcgc tcggggcttt gagagaggac tggaaccaga aaagatcatt ggggcaacag
     9541 attcctgtgg tgatttaatg ttcctaatga aatggaaaga cacagatgaa gctgacctgg
     9601 ttcttgcaaa agaagctaat gtgaaatgtc cacaaattgt gatagcattt tatgaagaga
     9661 gactgacatg gcatgcatat cctgaggatg cggaaaacaa agagaaagaa acagcaaaga
     9721 gctaacctgc aggcatgcaa gcttgatatc aagcttatcg ataatcaacc tctggattac
     9781 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     9841 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     9901 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     9961 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
    10021 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
    10081 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
    10141 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
    10201 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
    10261 tcccgcggcc
//
