LOCUS       sequence-343605-        4774 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  _C31 integrase CDS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4774)
  AUTHORS   Matinyan N, Karkhanis MS, Gonzalez Y, Jain A, Saltzman A, 
            Malovannaya A, Sarrion-Perdigones A, Dierick HA, Venken KJT
  TITLE     Multiplexed drug-based selection and counterselection genetic 
            manipulations in Drosophila.
  JOURNAL   Cell Rep. 2021 Sep 14;36(11):109700. doi: 
            10.1016/j.celrep.2021.109700.
  PUBMED    34525356
REFERENCE   2  (bases 1 to 4774)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-09-14"; volume: "36(11):109700. doi"; pages: " 
            10.1016/j.celrep.2021.109700"
COMMENT     Sequence Label: pFC31
FEATURES             Location/Qualifiers
     source          1..4774
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    23..34
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     primer_bind     complement(114..133)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(115..133)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(140..157)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(140..156)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      298..325
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     rep_origin      351..806
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(438..457)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     648..669
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        884..988
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(989..1057,1058..1849)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1207..1226)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2020..2608
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2509..2528
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2762..2779
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2794..2865)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2936..2952
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2936..2952
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        2968..2986
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2968..2985
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             join(3024..4774,1..67)
                     /codon_start=1
                     /product="integrase from phage phi-C31"
                     /label=phage phi-C31 integrase
                     /translation="MDTYAGAYDRQSRERENSSAASPATQRSANEDKAADLQREVERDG
                     GRFRFVGHFSEAPGTSAFGTAERPEFERILNECRAGRLNMIIVYDVSRFSRLKVMDAIP
                     IVSELLALGVTIVSTQEGVFRQGNVMDLIHLIMRLDASHKESSLKSAKILDTKNLQREL
                     GGYVGGKAPYGFELVSETKEITRNGRMVNVVINKLAHSTTPLTGPFEFEPDVIRWWWRE
                     IKTHKHLPFKPGSQAAIHPGSITGLCKRMDADAVPTRGETIGKKTASSAWDPATVMRIL
                     RDPRIAGFAAEVIYKKKPDGTPTTKIEGYRIQRDPITLRPVELDCGPIIEPAEWYELQA
                     WLDGRGRGKGLSRGQAILSAMDKLYCECGAVMTSKRGEESIKDSYRCRRRKVVDPSAPG
                     QHEGTCNVSMAALDKFVAERIFNKIRHAEGDEETLALLWEAARRFGKLTEAPEKSGERA
                     NLVAERADALNALEELYEDRAAGAYDGPVGRKHFRKQQAALTLRQQGAEERLAELEAAE
                     APKLPLDQWFPEDADADPTGPKSWWGRASVDDKRVFVGLFVDKIVVTKSTTGRGQGTPI
                     EKRASITWAKPPTDDDEDDAQDGTEDVAA"
ORIGIN
        1 ggctaagcct cctaccgacg acgatgagga tgacgcacag gacgggacag aggatgtagc
       61 agcctaagct tcgagacctt gagatcaata tctaaggtcc taagcggaat tcaccctata
      121 gtgagtcgta ttacaattca ctggccgtcg ttttacattg agaagagaaa agaaaaccgc
      181 cgatcctgtc caccgcatta ctgcaaggta gtggacaaga ccggcggtct taagtttttt
      241 ggctgaaagg gtggcgggca ggacgcccgc cataaactgc caggcatcaa attaagcaga
      301 aggccatcct gacggatggc ctttttgcgt ttctacaatg ttacgaatgg acgcgccctg
      361 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
      421 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
      481 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
      541 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc cagcgccctg
      601 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
      661 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
      721 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
      781 taacaaaata ttaacgctta caatttcctg atgcggtatt ttctccttac gcatctgtgc
      841 ggtatttcac accgcatcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
      901 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
      961 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     1021 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     1081 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     1141 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     1201 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     1261 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     1321 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     1381 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     1441 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     1501 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     1561 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     1621 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     1681 taaatctgga gccggtgagc gtgggtcgcg cggtatcatt gcagcactgg ggccagatgg
     1741 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     1801 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     1861 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     1921 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     1981 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     2041 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     2101 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     2161 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     2221 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     2281 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     2341 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     2401 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     2461 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     2521 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     2581 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     2641 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccatg attctgtgga
     2701 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     2761 cagcgagtca gtgagcgagg aagcggaagg tcagagagcg ttcaccgaca aacaacagat
     2821 aaaacgaaag gcccagtctt tcgactgagc ctttcgtttt atttgatgcc tgctcggtac
     2881 caaattccag aaaagaggcc tcccgaaagg ggggcctttt ttcgttttgg tccgacagga
     2941 aacagctatg accaacggct ctatggtatt taggtgacac tatagaacga attcactagt
     3001 gattgggcga tgagtggtct cgaatggaca cctatgccgg tgcctacgac agacagtccc
     3061 gggagcggga aaactccagt gcagccagcc ccgccacgca acggtcagct aacgaggata
     3121 aggcggccga cctgcagagg gaggtggaga gggacggcgg cagattcaga ttcgtaggcc
     3181 actttagcga ggcaccaggg actagtgcct ttgggacagc agagaggcca gagttcgaga
     3241 gaatcctgaa tgagtgccga gcgggccgcc tgaacatgat catagtttac gatgtttccc
     3301 ggttctcacg gctgaaggtt atggacgcca tccctatcgt aagtgagctg ctggcactcg
     3361 gggtgactat tgttagcact caggagggag tgttccggca gggaaatgtc atggacctga
     3421 ttcatttgat tatgcgcctg gacgccagcc acaaagaaag cagccttaag agcgcgaaaa
     3481 tcttggacac aaagaatctt cagcgagagc ttggcggcta cgtgggaggg aaggccccct
     3541 acgggttcga actcgttagc gagacaaaag aaatcaccag aaacggccga atggtcaacg
     3601 tcgtcattaa taaactggca cattccacaa ccccactgac tgggccattt gaattcgaac
     3661 ccgacgtgat ccgttggtgg tggcgtgaaa ttaagacgca caagcatctg ccctttaaac
     3721 ctggatctca ggcagccatt cacccgggct ccattactgg gctttgtaag cggatggacg
     3781 ccgacgcagt cccaacgagg ggggagacaa taggcaaaaa gactgcctca agcgcgtggg
     3841 accctgccac agtcatgcgc attctccggg atccccgcat tgccgggttc gccgcggagg
     3901 tgatatataa aaagaagccc gacggtacac ccactacaaa gattgagggc taccggatcc
     3961 agcgcgaccc cattaccctg cggccagtcg aactggattg cgggcctatt atcgaaccgg
     4021 cagaatggta cgaacttcag gcctggctgg atggacgggg gagaggcaaa gggctatcta
     4081 gaggccaggc tatcttgtcc gcgatggaca aactttattg cgaatgcggc gccgtcatga
     4141 catccaagag aggcgaagag agcatcaaag atagttatag atgcagacgt cgcaaagtgg
     4201 tcgatccctc agctcccgga cagcacgagg gcacctgtaa tgtcagtatg gccgcattag
     4261 acaagttcgt cgcagaacgt atctttaaca agatccgtca cgctgaagga gatgaagaaa
     4321 ccctggctct gttatgggag gccgccagac gatttgggaa gctgacagag gctccagaaa
     4381 aatctggcga acgtgccaac ctggtagctg agagagcgga tgcgctcaat gcactggagg
     4441 aactctacga ggatagggca gcgggcgcct acgatgggcc agtgggtcgc aagcacttcc
     4501 ggaaacagca ggcagcactg acacttagac aacagggcgc cgaggagagg ctcgccgagc
     4561 tagaggccgc tgaggccccg aagctccccc tggatcagtg gttccctgag gatgccgacg
     4621 ctgatcccac cggacctaaa agctggtggg ggcgagcttc cgtggacgat aaacgggttt
     4681 tcgtcggcct cttcgtggac aagatcgtgg tgactaagag caccactggc agagggcaag
     4741 gcacaccaat cgagaagcgc gcatctatca cttg
//
