LOCUS       sequence-343655-        9045 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Destination vector for AAVS1 targeting.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9045)
  AUTHORS   Araki M, Yoshimoto K, Ohta M, Katada T, Kontani K
  TITLE     Development of a versatile HPLC-based method to evaluate the 
            activation status of small GTPases.
  JOURNAL   J Biol Chem. 2021 Nov 18:101428. doi: 10.1016/j.jbc.2021.101428.
  PUBMED    34801548
REFERENCE   2  (bases 1 to 9045)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2021 Nov 18:101428. doi: 10.1016/j.jbc.2021.101428."
COMMENT     Sequence Label: pAAVS1_Puro_ccdb_Ubc_rtTA
FEATURES             Location/Qualifiers
     source          1..9045
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     259..278
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(481..1098,1099..1104,1105..1140,1141..1143,1144..1179,
                     1180..1182,1183..1218,1219..1227)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     polyA_signal    1277..1501
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    1521..2357
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     primer_bind     complement(2434..2451)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2605..3193)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2685..2704)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3364..4155,4156..4224))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3987..4006
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4225..4329)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4397..4415
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4453..4475)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4575..4594
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    4744..5547
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     misc_feature    5554..5579
                     /label=SA
                     /note="splice acceptor site"
     CDS             5603..5656
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5660..6256
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     6153..6173
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    6272..6393
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6283..6302)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6337..6356
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     protein_bind    6502..6626
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(6651..6656,6657..6674,6675..6681)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             6735..7394
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(6801..6820)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             7736..8041
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     7920..7939
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(8082..8206)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(8256..9045,1..422)
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
ORIGIN
        1 ccgttgggca gtgcacccgt acctttggga gcgcgcgccc tcgtcgtgtc gtgacgtcac
       61 ccgttctgtt ggcttataat gcagggtggg gccacctgcc ggtaggtgtg cggtaggctt
      121 ttctccgtcg caggacgcag ggttcgggcc tagggtaggc tctcctgaat cgacaggcgc
      181 cggacctctg gtgaggggag ggataagtga ggcgtcagtt tctttggtcg gttttatgta
      241 cctatcttct taagtagctg aagctccggt tttgaactat gcgctcgggg ttggcgagtg
      301 tgttttgtga agttttttag gcaccttttg aaatgtaatc atttgggtca atatgtaatt
      361 ttcagtgtta gactagtaaa ttgtccgcta aattctggcc gtttttggct tttttgttag
      421 acgaagcttg gtaccgagct cggatcccca ccccgtaccg gtcctgcagt cgaattcacc
      481 atgtctagac tggacaagag caaagtcata aacggagctc tggaattact caatggtgtc
      541 ggtatcgaag gcctgacgac aaggaaactc gctcaaaagc tgggagttga gcagcctacc
      601 ctgtactggc acgtgaagaa caagcgggcc ctgctcgatg ccctgccaat cgagatgctg
      661 gacaggcatc atacccactt ctgccccctg gaaggcgagt catggcaaga ctttctgcgg
      721 aacaacgcca agtcataccg ctgtgctctc ctctcacatc gcgacggggc taaagtgcat
      781 ctcggcaccc gcccaacaga gaaacagtac gaaaccctgg aaaatcagct cgcgttcctg
      841 tgtcagcaag gcttctccct ggagaacgca ctgtacgctc tgtccgccgt gggccacttt
      901 acactgggct gcgtattgga ggaacaggag catcaagtag caaaagagga aagagagaca
      961 cctaccaccg attctatgcc cccacttctg agacaagcaa ttgagctgtt cgaccggcag
     1021 ggagccgaac ctgccttcct tttcggcctg gaactaatca tatgtggcct ggagaaacag
     1081 ctaaagtgcg aaagcggcgg gccgaccgac gcccttgacg attttgactt agacatgctc
     1141 ccagccgatg cccttgacga ctttgacctt gatatgctgc ctgctgacgc tcttgacgat
     1201 tttgaccttg acatgctccc cgggtaacta agtaaggatc cgcggccgca ctagaggaat
     1261 tccgaaaccc tgcaggctgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     1321 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     1381 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     1441 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
     1501 ggtcgacagt actaagcttt actagggaca ggattggtga cagaaaagcc ccatccttag
     1561 gcctcctcct tcctagtctc ctgatattgg gtctaacccc cacctcctgt taggcagatt
     1621 ccttatctgg tgacacaccc ccatttcctg gagccatctc tctccttgcc agaacctcta
     1681 aggtttgctt acgatggagc cagagaggat cctgggaggg agagcttggc agggggtggg
     1741 agggaagggg gggatgcgtg acctgcccgg ttctcagtgg ccaccctgcg ctaccctctc
     1801 ccagaacctg agctgctctg acgcggctgt ctggtgcgtt tcactgatcc tggtgctgca
     1861 gcttccttac acttcccaag aggagaagca gtttggaaaa acaaaatcag aataagttgg
     1921 tcctgagttc taactttggc tcttcacctt tctagtcccc aatttatatt gttcctccgt
     1981 gcgtcagttt tacctgtgag ataaggccag tagccagccc cgtcctggca gggctgtggt
     2041 gaggaggggg gtgtccgtgt ggaaaactcc ctttgtgaga atggtgcgtc ctaggtgttc
     2101 accaggtcgt ggccgcctct actccctttc tctttctcca tccttctttc cttaaagagt
     2161 ccccagtgct atctgggaca tattcctccg cccagagcag ggtcccgctt ccctaaggcc
     2221 ctgctctggg cttctgggtt tgagtccttg gcaagcccag gagaggcgct caggcttccc
     2281 tgtccccctt cctcgtccac catctcatgc ccctggctct cctgcccctt ccctacaggg
     2341 gttcctggct ctgctctaag ggcgaattct gcattaatga atcggccaac gcgcggggag
     2401 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     2461 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     2521 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     2581 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     2641 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     2701 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     2761 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     2821 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     2881 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     2941 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     3001 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     3061 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     3121 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     3181 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     3241 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     3301 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     3361 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     3421 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     3481 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     3541 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     3601 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     3661 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     3721 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     3781 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     3841 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     3901 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     3961 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     4021 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     4081 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     4141 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     4201 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     4261 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     4321 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
     4381 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga
     4441 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
     4501 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
     4561 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga
     4621 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct
     4681 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agaattcgcc
     4741 ctttgctttc tctgaccagc attctctccc ctgggcctgt gccgctttct gtctgcagct
     4801 tgtggcctgg gtcacctcta cggctggccc agatccttcc ctgccgcctc cttcaggttc
     4861 cgtcttcctc cactccctct tccccttgct ctctgctgtg ttgctgccca aggatgctct
     4921 ttccggagca cttccttctc ggcgctgcac cacgtgatgt cctctgagcg gatcctcccc
     4981 gtgtctgggt cctctccggg catctctcct ccctcaccca accccatgcc gtcttcactc
     5041 gctgggttcc cttttccttc tccttctggg gcctgtgcca tctctcgttt cttaggatgg
     5101 ccttctccga cggatgtctc ccttgcgtcc cgcctcccct tcttgtaggc ctgcatcatc
     5161 accgtttttc tggacaaccc caaagtaccc cgtctccctg gctttagcca cctctccatc
     5221 ctcttgcttt ctttgcctgg acaccccgtt ctcctgtgga ttcgggtcac ctctcactcc
     5281 tttcatttgg gcagctcccc tacccccctt acctctctag tctgtgctag ctcttccagc
     5341 cccctgtcat ggcatcttcc aggggtccga gagctcagct agtcttcttc ctccaacccg
     5401 ggcccctatg tccacttcag gacagcatgt ttgctgcctc cagggatcct gtgtccccga
     5461 gctgggacca ccttatattc ccagggccgg ttaatgtggc tctggttctg ggtactttta
     5521 tctgtcccct ccaccccaca gtggggcaag cttctgacct cttctcttcc tcccacaggg
     5581 cctcgagaga tctggcggcg gagagggcag aggaagtctt ctaacatgcg gtgacgtgga
     5641 ggagaatccc ggccctagca ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga
     5701 cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca
     5761 caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac
     5821 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt
     5881 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat
     5941 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc
     6001 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca
     6061 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg
     6121 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg
     6181 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg
     6241 caagcccggt gcctgatcta gacagacatg ataagataca ttgatgagtt tggacaaacc
     6301 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta
     6361 tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg
     6421 tttcaggttc agggggaggt gtgggaggtt ttttaacagg gacagcagag atccagtttg
     6481 gttaattcta gacagttatc aacaagtttg tacaaaaaag ctgaacgaga aacgtaaaat
     6541 gatataaata tcaatatatt aaattagatt ttgcataaaa aacagactac ataatactgt
     6601 aaaacacaac atatccagtc actatggcgg ccgcattagg caccccaggc tttacacttt
     6661 atgcttccgg ctcgtataat gtgtggattt tgagttagga tccgtcgaga ttttcaggag
     6721 ctaaggaagc taaaatggag aaaaaaatca ctggatatac caccgttgat atatcccaat
     6781 ggcatcgtaa agaacatttt gaggcatttc agtcagttgc tcaatgtacc tataaccaga
     6841 ccgttcagct ggatattacg gcctttttaa agaccgtaaa gaaaaataag cacaagtttt
     6901 atccggcctt tattcacatt cttgcccgcc tgatgaatgc tcatccggaa ttccgtatgg
     6961 caatgaaaga cggtgagctg gtgatatggg atagtgttca cccttgttac accgttttcc
     7021 atgagcaaac tgaaacgttt tcatcgctct ggagtgaata ccacgacgat ttccggcagt
     7081 ttctacacat atattcgcaa gatgtggcgt gttacggtga aaacctggcc tatttcccta
     7141 aagggtttat tgagaatatg tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt
     7201 ttgatttaaa cgtggccaat atggacaact tcttcgcccc cgttttcacc atgggcaaat
     7261 attatacgca aggcgacaag gtgctgatgc cgctggcgat tcaggttcat catgccgttt
     7321 gtgatggctt ccatgtcggc agaatgctta atgaattaca acagtactgc gatgagtggc
     7381 agggcggggc gtaaagatct ggatccggct tactaaaagc cagataacag tatgcgtatt
     7441 tgcgcgctga tttttgcggt ataagaatat atactgatat gtatacccga agtatgtcaa
     7501 aaagaggtat gctatgaagc agcgtattac agtgacagtt gacagcgaca gctatcagtt
     7561 gctcaaggca tatatgatgt caatatctcc ggtctggtaa gcacaaccat gcagaatgaa
     7621 gcccgtcgtc tgcgtgccga acgctggaaa gcggaaaatc aggaagggat ggctgaggtc
     7681 gcccggttta ttgaaatgaa cggctctttt gctgacgaga acaggggctg gtgaaatgca
     7741 gtttaaggtt tacacctata aaagagagag ccgttatcgt ctgtttgtgg atgtacagag
     7801 tgatattatt gacacgcccg ggcgacggat ggtgatcccc ctggccagtg cacgtctgct
     7861 gtcagataaa gtctcccgtg aactttaccc ggtggtgcat atcggggatg aaagctggcg
     7921 catgatgacc accgatatgg ccagtgtgcc ggtctccgtt atcggggaag aagtggctga
     7981 tctcagccac cgcgaaaatg acatcaaaaa cgccattaac ctgatgttct ggggaatata
     8041 aatgtcaggc tcccttatac acagccagtc tgcaggtcga ccatagtgac tggatatgtt
     8101 gtgttttaca gtattatgta gtctgttttt tatgcaaaat ctaatttaat atattgatat
     8161 ttatatcatt ttacgtttct cgttcagctt tcttgtacaa agtggttgat aacatctcga
     8221 gccataaaga tggttaatta acccacccaa gatctggcct ccgcgccggg ttttggcgcc
     8281 tcccgcgggc gcccccctcc tcacggcgag cgctgccacg tcagacgaag ggcgcagcga
     8341 gcgtcctgat ccttccgccc ggacgctcag gacagcggcc cgctgctcat aagactcggc
     8401 cttagaaccc cagtatcagc agaaggacat tttaggacgg gacttgggtg actctagggc
     8461 actggttttc tttccagaga gcggaacagg cgaggaaaag tagtcccttc tcggcgattc
     8521 tgcggaggga tctccgtggg gcggtgaacg ccgatgatta tataaggacg cgccgggtgt
     8581 ggcacagcta gttccgtcgc agccgggatt tgggtcgcgg ttcttgtttg tggatcgctg
     8641 tgatcgtcac ttggtgagta gcgggctgct gggctggccg gggctttcgt ggccgccggg
     8701 ccgctcggtg ggacggaagc gtgtggagag accgccaagg gctgtagtct gggtccgcga
     8761 gcaaggttgc cctgaactgg gggttggggg gagcgcagca aaatggcggc tgttcccgag
     8821 tcttgaatgg aagacgcttg tgaggcgggc tgtgaggtcg ttgaaacaag gtggggggca
     8881 tggtgggcgg caagaaccca aggtcttgag gccttcgcta atgcgggaaa gctcttattc
     8941 gggtgagatg ggctggggca ccatctgggg accctgacgt gaagtttgtc actgactgga
     9001 gaactcggtt tgtcgtctgt tgcgggggcg gcagttatgg cggtg
//
