LOCUS       sequence-343684-       10603 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses GFP-MYH9 construct (human myosin IIA) carrying S1943A 
            mutation and S1916A, S1915A mutation, driven by CMV promoter. Thus 
            inhibits CKII and PKC phosphorylation of the tail..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10603)
  AUTHORS   Rai V, Thomas DG, Beach JR, Egelhoff TT
  TITLE     Myosin IIA Heavy Chain Phosphorylation Mediates Adhesion Maturation 
            and Protrusion in Three Dimensions.
  JOURNAL   J Biol Chem. 2017 Feb 24;292(8):3099-3111. doi: 
            10.1074/jbc.M116.733402. Epub 2017 Jan 4.
  PUBMED    28053086
REFERENCE   2  (bases 1 to 10603)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M116.733402"; journalName: "J Biol Chem"; date: 
            "2017-02-24- 24"; volume: "292"; issue: "8"; pages: "3099-3111"
COMMENT     Sequence Label: pEGFP-NMHC-IIA-3xA
FEATURES             Location/Qualifiers
     source          1..10603
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        91..394
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        395..598
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     549..569
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      637..646
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(643..645,646..648,649..1359)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(643..645,646..648,649..1359)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(688..709)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(949..968)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1296..1317
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    7421..7542
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7458..7477)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7512..7531
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(7549..8004)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7686..7707)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7898..7917
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        8031..8135
                     /gene="bla"
                     /label=AmpR promoter
     promoter        8137..8494
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        8137..8328
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(8160..8180)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      8345..8480
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8529..9323
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8529..9323
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8583..8602)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9193..9212
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(9511..9530)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    9555..9602
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      9931..10519
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10420..10439
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tctgtggata accgtattac cgccatgcat tagttattaa tagtaatcaa ttacggggtc
       61 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
      121 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
      181 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
      241 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
      301 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
      361 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa
      421 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
      481 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc
      541 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctgg
      601 tttagtgaac cgtcagatcc gctagcgcta ccggtcgcca ccatggtgag caagggcgag
      661 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
      721 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
      781 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc
      841 tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag
      901 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
      961 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
     1021 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
     1081 aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc
     1141 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
     1201 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc
     1261 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     1321 gccgccggga tcactctcgg catggacgag ctgtacaagt actcagatct cgagctcaag
     1381 cttatggcac agcaagctgc cgataagtat ctctatgtgg ataaaaactt catcaacaat
     1441 ccgctggccc aggccgactg ggctgccaag aagctggtat gggtgccttc cgacaagagt
     1501 ggctttgagc cagccagcct caaggaggag gtgggcgaag aggccatcgt ggagctggtg
     1561 gagaatggga agaaggtgaa ggtgaacaag gatgacatcc agaagatgaa cccgcccaag
     1621 ttctccaagg tggaggacat ggcagagctc acgtgcctca acgaagcctc ggtgctgcac
     1681 aacctcaagg agcgttacta ctcagggctc atctacacct attcaggcct gttctgtgtg
     1741 gtcatcaatc cttacaagaa cctgcccatc tactctgaag agattgtgga aatgtacaag
     1801 ggcaagaaga ggcacgagat gccccctcac atctatgcca tcacagacac cgcctacagg
     1861 agtatgatgc aagaccgaga agatcaatcc atcttgtgca ctggtgaatc tggagctggc
     1921 aagacggaga acaccaagaa ggtcatccag tatctggcgt acgtggcgtc ctcgcacaag
     1981 agcaagaagg accagggcga gctggagcgg cagctgctgc aggccaaccc catcctggag
     2041 gccttcggga acgccaagac cgtgaagaat gacaactcct cccgcttcgg caaattcatt
     2101 cgcatcaact ttgatgtcaa tggctacatt gttggagcca acattgagac ttatcttttg
     2161 gagaaatctc gtgctatccg ccaagccaag gaagaacgga ccttccacat cttctattat
     2221 ctcctgtctg gggctggaga gcacctgaag accgatctcc tgttggagcc gtacaacaaa
     2281 taccgcttcc tgtccaatgg acacgtcacc atccccgggc agcaggacaa ggacatgttc
     2341 caggagacca tggaggccat gaggattatg ggcatcccag aagaggagca aatgggcctg
     2401 ctgcgggtca tctcaggggt tcttcagctc ggcaacatcg tcttcaagaa ggagcggaac
     2461 actgaccagg cgtccatgcc cgacaacaca gctgcccaaa aggtgtccca tctcttgggt
     2521 atcaatgtga ccgatttcac cagaggaatc ctcaccccgc gcatcaaggt gggacgggat
     2581 tacgtccaga aggcgcagac taaagagcag gctgactttg ccatcgaggc cttggccaag
     2641 gcgacctatg agcggatgtt ccgctggctg gtgctgcgca tcaacaaggc tctggacaag
     2701 accaagaggc agggcgcctc cttcatcggg atcctggaca ttgccggctt cgagatcttt
     2761 gatctgaact cgtttgagca gctgtgcatc aattacacca atgagaagct gcagcagctc
     2821 ttcaaccaca ccatgttcat cctggagcag gaggagtacc agcgcgaggg catcgagtgg
     2881 aacttcatcg actttggcct cgacctgcag ccctgcatcg acctcattga gaagccagca
     2941 ggccccccgg gcattctggc cctgctggac gaggagtgct ggttccccaa agccaccgac
     3001 aagagcttcg tggagaaggt gatgcaggag cagggcaccc accccaagtt ccagaagccc
     3061 aagcagctga aggacaaagc tgatttctgc attatccact atgccggcaa ggtggattac
     3121 aaagctgacg agtggctgat gaagaacatg gatcccctga atgacaacat cgccacactg
     3181 ctccaccagt cctctgacaa gtttgtctcg gagctgtgga aggatgtgga ccgcatcatc
     3241 ggcctggacc aggtggccgg catgtcggag accgcactgc ccggggcctt caagacgcgg
     3301 aagggcatgt tccgcactgt ggggcagctt tacaaggagc agctggccaa gctgatggct
     3361 acgctgagga acacgaaccc caactttgtc cgctgcatca tccccaacca cgagaagaag
     3421 gccggcaagc tggacccgca tctcgtgctg gaccagctgc gctgcaacgg tgttctcgag
     3481 ggcatccgta tctgccgcca gggcttcccc aacagggtgg tcttccagga gtttcggcag
     3541 agatatgaga tcctgactcc aaactccatt cccaagggtt tcatggacgg gaagcaggcg
     3601 tgcgtgctca tgataaaagc cctggagctc gacagcaatc tgtaccgcat tggccagagc
     3661 aaagtcttct tccgtgccgg tgtgctggcc cacctggagg aggagcgaga cctgaagatc
     3721 accgacgtca tcatagggtt ccaggcctgc tgcaggggct acctggccag gaaagcattt
     3781 gccaagcggc agcagcagct taccgccatg aaggtcctcc agcggaactg cgctgcctac
     3841 ctgaagctgc ggaactggca gtggtggcgg ctcttcacca aggtcaagcc gctgctgcag
     3901 gtgagccggc aggaggagga gatgatggcc aaggaggagg agctggtgaa ggtcagagag
     3961 aagcagctgg ctgcggagaa caggctcacg gagatggaga cgctgcagtc tcagctcatg
     4021 gcagagaaat tgcagctgca ggagcagctc caggcagaaa ccgagctgtg tgccgaggct
     4081 gaggagctcc gggcccgcct gaccgccaag aagcaggaat tagaagagat ctgccatgac
     4141 ctagaggcca gggtggagga ggaggaggag cgctgccagc acctgcaggc ggagaagaag
     4201 aagatgcagc agaacatcca ggagcttgag gagcagctgg aggaggagga gagcgcccgg
     4261 cagaagctgc agctggagaa ggtgaccacc gaggcgaagc tgaaaaagct ggaggaggag
     4321 cagatcatcc tggaggacca gaactgcaag ctggccaagg aaaagaaact gctggaagac
     4381 agaatagctg agttcaccac caacctcaca gaagaggagg agaaatctaa gagcctcgcc
     4441 aagctcaaga acaagcatga ggcaatgatc actgacttgg aagagcgcct ccgcagggag
     4501 gagaagcagc gacaggagct ggagaagacc cgccggaagc tggagggaga ctccacagac
     4561 ctcagcgacc agatcgccga gctccaggcc cagatcgcgg agctcaagat gcagctggcc
     4621 aagaaagagg aggagctcca ggccgccctg gccagagtgg aagaggaagc tgcccagaag
     4681 aacatggccc tcaagaagat ccgggagctg gaatctcaga tctctgaact ccaggaagac
     4741 ctggagtctg agcgtgcttc caggaataaa gctgagaagc agaaacggga ccttggggaa
     4801 gagctagagg cgctgaaaac agagttggag gacacgctgg attccacagc tgcccagcag
     4861 gagctcaggt caaaacgtga gcaggaggtg aacatcctga agaagaccct ggaggaggag
     4921 gccaagaccc acgaggccca gatccaggag atgaggcaga agcactcaca ggccgtggag
     4981 gagctggcgg agcagctgga gcagacgaag cgggtgaaag caaacctcga gaaggcaaag
     5041 cagactctgg agaacgagcg gggggagctg gccaacgagg tgaaggtgct gctgcagggc
     5101 aaaggggact cggagcacaa gcgcaagaaa gtggaggcgc agctgcagga gctgcaggtc
     5161 aagttcaacg agggagagcg cgtgcgcaca gagctggccg acaaggtcac caagctgcag
     5221 gtggagctgg acaacgtgac cgggcttctc agccagtccg acagcaagtc cagcaagctc
     5281 accaaggact tctccgcgct ggagtcccag ctgcaggaca ctcaggagct gctgcaggag
     5341 gagaaccggc agaagctgag cctgagcacc aagctcaagc aggtggagga cgagaagaat
     5401 tccttccggg agcagctgga ggaggaggag gaggccaagc acaacctgga gaagcagatc
     5461 gccaccctcc atgcccaggt ggccgacatg aaaaagaaga tggaggacag tgtggggtgc
     5521 ctggaaactg ctgaggaggt gaagaggaag ctccagaagg acctggaggg cctgagccag
     5581 cggcacgagg agaaggtggc cgcctacgac aagctggaga agaccaagac gcggctgcag
     5641 caggagctgg acgacctgct ggtggacctg gaccaccagc gccagagcgc gtgcaacctg
     5701 gagaagaagc agaagaagtt tgaccagctc ctggcggagg agaagaccat ctctgccaag
     5761 tatgcagagg agcgcgaccg ggctgaggcg gaggcccgag agaaggagac caaggctctg
     5821 tcgctggccc gggccctgga ggaagccatg gagcagaagg cggagctgga gcggctcaac
     5881 aagcagttcc gcacggagat ggaggacctt atgagctcca aggatgatgt gggcaagagt
     5941 gtccacgagc tggagaagtc caagcgggcc ctagagcagc aggtggagga gatgaagacg
     6001 cagctggaag agctggagga cgagctgcag gccaccgaag atgccaagct gcggttggag
     6061 gtcaacctgc aggccatgaa ggcccagttc gagcgggacc tgcagggccg ggacgagcag
     6121 agcgaggaga agaagaagca gctggtcaga caggtgcggg agatggaggc agagctggag
     6181 gacgagagga agcagcgctc gatggcagtg gccgcccgga agaagctgga gatggacctg
     6241 aaggacctgg aggcgcacat cgactcggcc aacaagaacc gggacgaagc catcaaacag
     6301 ctgcggaagc tgcaggccca gatgaaggac tgcatgcgcg agctggatga cacccgcgcc
     6361 tctcgtgagg agatcctggc ccaggccaaa gagaacgaga agaagctgaa gagcatggag
     6421 gccgagatga tccagttgca ggaggaactg gcagccgcgg agcgtgccaa gcgccaggcc
     6481 cagcaggagc gggatgagct ggctgacgag atcgccaaca gcagcggcaa aggagccctg
     6541 gcgttagagg agaagcggcg tctggaggcc cgcatcgccc agctggagga ggagctggag
     6601 gaggagcagg gcaacacgga gctgatcaac gaccggctga agaaggccaa cctgcagatc
     6661 gaccagatca acaccgacct gaacctggag cgcagccacg cccagaagaa cgagaatgct
     6721 cggcagcagc tggaacgcca gaacaaggag cttaaggtca agctgcagga gatggagggc
     6781 actgtcaagt ccaagtacaa ggcctccatc accgccctcg aggccaagat tgcacagctg
     6841 gaggagcagc tggacaacga gaccaaggag cgccaggcag cctgcaaaca ggtgcgtcgg
     6901 accgagaaga agctgaagga tgtgctgctg caggtggatg acgagcggag gaacgccgag
     6961 cagtacaagg accaggccga caaggcatct acccgcctga agcagctcaa gcggcagctg
     7021 gaggaggccg aagaggaggc ccagcgggcc aacgcctccc gccggaaact gcagcgcgag
     7081 ctggaggacg ccactgagac ggccgatgcc atgaaccgcg aagtcgccgc cctaaagaac
     7141 aagctcaggc gcggggacct gccgtttgtc gtgccccgcc gaatggcccg gaaaggcgcc
     7201 ggggatggcg ccgacgaaga ggtagatggc aaagcggatg gggctgaggc caaacctgcc
     7261 gaataaacta gtcgacggta ccgcgggccc gggatccacc ggatctagat aactgatcat
     7321 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
     7381 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
     7441 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     7501 gcattctagt tgtggtttgt ccaaactcat caatgtatct taacgcgtaa attgtaagcg
     7561 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
     7621 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
     7681 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
     7741 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
     7801 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
     7861 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
     7921 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
     7981 ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     8041 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     8101 gataaatgct tcaataatat tgaaaaagga agagtcctga ggcggaaaga accagctgtg
     8161 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     8221 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     8281 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     8341 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     8401 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     8461 aggaggcttt tttggaggcc taggcttttg caaagatcga tcaagagaca ggatgaggat
     8521 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
     8581 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     8641 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     8701 atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     8761 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     8821 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
     8881 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     8941 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     9001 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca
     9061 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
     9121 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     9181 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     9241 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     9301 gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac
     9361 gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
     9421 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
     9481 gttcttcgcc caccctaggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg
     9541 aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt
     9601 tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca
     9661 ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga
     9721 aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcct caggttactc
     9781 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     9841 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     9901 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     9961 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
    10021 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
    10081 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
    10141 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
    10201 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
    10261 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
    10321 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
    10381 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
    10441 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
    10501 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
    10561 ctggcctttt gctcacatgt tctttcctgc gttatcccct gat
//
