LOCUS       sequence-343789-        4349 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4349)
  AUTHORS   Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel 
            A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, 
            Falter-Braun P
  TITLE     A resource of human coronavirus protein-coding sequences in a 
            flexible, multipurpose Gateway Entry clone collection.
  JOURNAL   G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 
            10.1093/g3journal/jkad105.
  PUBMED    37267226
REFERENCE   2  (bases 1 to 4349)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2023-07-5"; volume: "13(7):jkad105. doi"; 
            pages: " 10.1093/g3journal/jkad105"
COMMENT     Sequence Label: pEntry_SARS-CoV-2-nsp4
FEATURES             Location/Qualifiers
     source          1..4349
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1660..1759)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1776..1795)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1777..1795)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1800..1816)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1800..1816)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2247..3038
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3131..3719
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3620..3639
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3873..3890
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4049..4076)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4168..4254)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4303..4325
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4317..4334
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4318..4334
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcagaag ggccgtcaag gccaggatcc
      121 atgggatcca aaattgttaa taattggttg aagcagttga tcaaagttac acttgtgttc
      181 ctttttgttg ctgctatttt ctatttaata acacctgttc atgtcatgtc taaacatact
      241 gacttttcaa gtgaaatcat aggatacaag gctattgatg gtggtgtcac tcgtgacata
      301 gcatctacag atacttgttt tgctaacaaa catgctgatt ttgacacatg gtttagccag
      361 cgtggtggta gttatactaa tgacaaagct tgcccattga ttgctgcagt cataacaaga
      421 gaagtgggtt ttgtcgtgcc tggtttgcct ggcacgatat tacgcacaac taatggtgac
      481 tttttgcatt tcttacctag agtttttagt gcagttggta acatctgtta cacaccatca
      541 aaacttatag agtacactga ctttgcaaca tcagcttgtg ttttggctgc tgaatgtaca
      601 atttttaaag atgcttctgg taagccagta ccatattgtt atgataccaa tgtactagaa
      661 ggttctgttg cttatgaaag tttacgccct gacacacgtt atgtgctcat ggatggctct
      721 attattcaat ttcctaacac ctaccttgaa ggttctgtta gagtggtaac aacttttgat
      781 tctgagtact gtaggcacgg cacttgtgaa agatcagaag ctggtgtttg tgtatctact
      841 agtggtagat gggtacttaa caatgattat tacagatctt taccaggagt tttctgtggt
      901 gtagatgctg taaatttact tactaatatg tttacaccac taattcaacc tattggtgct
      961 ttggacatat cagcatctat agtagctggt ggtattgtag ctatcgtagt aacatgcctt
     1021 gcctactatt ttatgaggtt tagaagagct tttggtgaat acagtcatgt agttgccttt
     1081 aatactttac tattccttat gtcattcact gtactctgtt taacaccagt ttactcattc
     1141 ttacctggtg tttattctgt tatttacttg tacttgacat tttatcttac taatgatgtt
     1201 tcttttttag cacatattca gtggatggtt atgttcacac ctttagtacc tttctggata
     1261 acaattgctt atatcatttg tatttccaca aagcatttct attggttctt tagtaattac
     1321 ctaaagagac gtgtagtctt taatggtgtt tcctttagta cttttgaaga agctgcgctg
     1381 tgcacctttt tgttaaataa agaaatgtat ctaaagttgc gtagtgatgt gctattacct
     1441 cttacgcaat ataatagata cttagctctt tataataagt acaagtattt tagtggagca
     1501 atggatacaa ctagctacag agaagctgct tgttgtcatc tcgcaaaggc tctcaatgac
     1561 ttcagtaact caggttctga tgttctttac caaccaccac aaacctctat cacctcagct
     1621 gttttgcagc ttaattaaga cgcgatcgcg gcctcatggg cccagctttc ttgtacaaag
     1681 ttggcattat aaaaaataat tgctcatcaa tttgttgcaa cgaacaggtc actatcagtc
     1741 aaaataaaat cattatttgc catccagctg atatccccta tagtgagtcg tattacatgg
     1801 tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt tacattgcac
     1861 aagataaaaa tatatcatca tgcctcctct agaccagcca ggacagaaat gcctcgactt
     1921 cgctgctacc caaggttgcc gggtgacgca caccgtggaa acggatgaag gcacgaaccc
     1981 agtggacata agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg cgctcacgca
     2041 actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg
     2101 cttgttatga ctgttttttt ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc
     2161 gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagggca
     2221 gtcgccctaa aacaaagtta aacattatga gggaagcggt gatcgccgaa gtatcgactc
     2281 aactatcaga ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac
     2341 atttgtacgg ctccgcagtg gatggcggcc tgaagccaca cagtgatatt gatttgctgg
     2401 ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg
     2461 aaacttcggc ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg
     2521 tgcacgacga catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat
     2581 ggcagcgcaa tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg
     2641 ctatcttgct gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg
     2701 aactctttga tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc
     2761 tatggaactc gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc
     2821 gcatttggta cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg
     2881 caatggagcg cctgccggcc cagtatcagc ccgtcatact tgaagctaga caggcttatc
     2941 ttggacaaga agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgtccact
     3001 acgtgaaagg cgagatcacc aaggtagtcg gcaaataacc ctcgagccac ccatgaccaa
     3061 aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc agaccccgta gaaaagatca
     3121 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     3181 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     3241 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     3301 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     3361 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     3421 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     3481 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     3541 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     3601 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     3661 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     3721 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     3781 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     3841 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     3901 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     3961 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat acgcgtaccg
     4021 ctagccagga agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt
     4081 agtttgatgc ctggcagttt atggcgggcg tcctgcccgc caccctccgg gccgttgctt
     4141 cacaacgttc aaatccgctc ccggcggatt tgtcctactc aggagagcgt tcaccgacaa
     4201 acaacagata aaacgaaagg cccagtcttc cgactgagcc tttcgtttta tttgatgcct
     4261 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
     4321 aaacgacggc cagtcttaag ctcgggccc
//
