LOCUS       sequence-343891-       12400 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  overexpression of RanCas13b in human cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12400)
  AUTHORS   Li S, Li X, Xue W, Zhang L, Yang LZ, Cao SM, Lei YN, Liu CX, Guo SK,
            Shan L, Wu M, Tao X, Zhang JL, Gao X, Zhang J, Wei J, Li J, Yang L, 
            Chen LL
  TITLE     Screening for functional circular RNAs using the CRISPR-Cas13 
            system.
  JOURNAL   Nat Methods. 2021 Jan;18(1):51-59. doi: 10.1038/s41592-020-01011-4. 
            Epub 2020 Dec 7.
  PUBMED    33288960
REFERENCE   2  (bases 1 to 12400)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-020-01011-4"; journalName: "Nat Methods"; date: 
            "2021-01"; volume: "18"; issue: "1"; pages: "51-59"
COMMENT     Sequence Label: p23-NES-RanCas13b-msfGFP-NES-Flag
FEATURES             Location/Qualifiers
     source          1..12400
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             join(4514..4516,4517..4519,4520..5230)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(4559..4580)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     5167..5188
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5240..5266
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             5291..5356
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    5363..5930
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5524..5541)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5751..5770
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5950..6549
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5950..5969)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6446..6466
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6568..7156
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6621..6641)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7039..7050)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7231..7464
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7583..7601)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7669..7773
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7774..7842,7843..8634)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7992..8011)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8805..9393
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9294..9313
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9547..9564
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9804..9825
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9840..9845,9846..9863,9864..9870)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9878..9894
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9883..9905
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9902..9918
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9902..9918
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9968..9987
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             10179..10812
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10859..10984
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11481..11714
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11899..11943
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    12236..12353
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgaa gcggccatgc tgtaccctga
     1201 gcggctgcgg cggatcctga ccgctagcga gaagcccctg ctgcctaacg tgtataccct
     1261 gaagcacaag ttcttttggg gcgccttcct gaatatcgcc cggcacaacg cctttatcac
     1321 catctgccac atcaatgagc agctgggcct gaaaaccccc agcaacgacg ataagatcgt
     1381 ggacgtggtg tgcgagacat ggaacaatat cctgaacaat gaccacgatc tgctgaagaa
     1441 gtcccagctg accgagctga tcctgaagca cttccctttt ctgacagcca tgtgctacca
     1501 cccccctaag aaggagggca agaagaaggg ccaccagaag gagcagcaga aggagaagga
     1561 gagcgaggca cagtcccagg cagaggccct gaacccatcc aagctgatcg aggccctgga
     1621 gatcctggtg aatcagctgc actctctgcg gaactactat agccactata agcacaagaa
     1681 gcccgacgcc gagaaggata tcttcaagca cctgtataag gccttcgacg cctctctgag
     1741 aatggtgaag gaggattata aggcccactt caccgtgaat ctgacaaggg actttgccca
     1801 cctgaaccgc aagggcaaga acaagcagga caatcccgac ttcaacagat acagattcga
     1861 gaaggatggc ttctttaccg agtctggcct gctgttcttt acaaatctgt ttctggacaa
     1921 gagggatgcc tattggatgc tgaagaaggt gtccggcttc aaggcctctc acaagcagcg
     1981 cgagaagatg accacagagg tgttttgcag gagccgcatc ctgctgccca agctgaggct
     2041 ggagtcccgc tacgaccaca accagatgct gctggatatg ctgtctgagc tgagcagatg
     2101 tcctaagctg ctgtatgaga agctgagcga ggagaataag aagcacttcc aggtggaggc
     2161 cgacggcttt ctggatgaga tcgaggagga gcagaaccca ttcaaggaca ccctgatccg
     2221 gcaccaggat agattcccct actttgccct gaggtatctg gacctgaatg agtccttcaa
     2281 gtctatccgc tttcaggtgg atctgggcac ataccactat tgtatctacg acaagaagat
     2341 cggcgatgag caggagaaga ggcacctgac ccgcacactg ctgtccttcg gccggctgca
     2401 ggactttacc gagatcaaca gaccccagga gtggaaggcc ctgaccaagg acctggatta
     2461 caaggagaca tccaatcagc ctttcatctc taagaccaca ccacactatc acatcaccga
     2521 caacaagatc ggctttcggc tgggcacaag caaggagctg tacccctccc tggagatcaa
     2581 ggatggcgcc aatagaatcg ccaagtaccc ttataactcc ggcttcgtgg cccacgcctt
     2641 tatctctgtg cacgagctgc tgcctctgat gttctaccag cacctgaccg gcaagagcga
     2701 ggacctgctg aaggagacag tgcggcacat ccagagaatc tataaggact tcgaggagga
     2761 gcggatcaat accatcgagg atctggagaa ggcaaaccag ggcagactgc cactgggagc
     2821 ctttcccaag cagatgctgg gcctgctgca gaataagcag cctgatctgt ccgagaaggc
     2881 caagatcaag atcgagaagc tgatcgccga gacaaagctg ctgtctcaca ggctgaacac
     2941 aaagctgaag agctccccaa agctgggcaa gcggagagag aagctgatca agacaggcgt
     3001 gctggccgac tggctggtga aggatttcat gcgctttcag cccgtggcct acgacgccca
     3061 gaatcagcct atcaagtcta gcaaggccaa ctccaccgag ttctggttta tcaggcgcgc
     3121 cctggccctg tacggaggag agaagaatag gctggagggc tatttcaagc agaccaacct
     3181 gatcggcaac acaaatccac accccttcct gaacaagttt aattggaagg cctgcaggaa
     3241 tctggtggat ttctaccagc agtatctgga gcagcgcgag aagtttctgg aggccatcaa
     3301 gaaccagcca tgggagccct accagtattg cctgctgctg aagatcccta aggagaacag
     3361 gaagaatctg gtgaagggat gggagcaggg aggaatctct ctgccacggg gcctgtttac
     3421 cgaggccatc agagagacac tgtctgagga cctgatgctg agcaagccaa tccgcaagga
     3481 gatcaagaag cacggccggg tgggcttcat cagcagagcc atcaccctgt actttaagga
     3541 gaagtatcag gataagcacc agagcttcta caatctgtcc tataagctgg aggcaaaggc
     3601 accactgctg aagcgggagg agcactacga gtattggcag cagaacaagc ctcagtctcc
     3661 aaccgagagc cagaggctgg agctgcacac aagcgaccgc tggaaggatt acctgctgta
     3721 taagagatgg cagcacctgg agaagaagct gcggctgtac cggaatcagg acgtgatgct
     3781 gtggctgatg accctggagc tgacaaagaa ccacttcaag gagctgaacc tgaattatca
     3841 ccagctgaag ctggagaatc tggccgtgaa cgtgcaggag gccgatgcca agctgaaccc
     3901 tctgaatcag accctgccca tggtgctgcc tgtgaaggtg taccctgcca cagcctttgg
     3961 cgaggtgcag taccacaaga ccccaatcag gacagtgtat atccgcgagg agcacaccaa
     4021 ggccctgaag atgggcaact tcaaggccct ggtgaaggac cggagactga atggcctgtt
     4081 ctcttttatc aaggaggaga acgatacaca gaagcaccca atcagccagc tgcggctgag
     4141 gcgcgagctg gagatctacc agtctctgag agtggacgcc ttcaaggaga cactgagcct
     4201 ggaggagaag ctgctgaata agcacacatc cctgtcctct ctggagaacg agtttagggc
     4261 cctgctggag gagtggaaga aggagtatgc cgccagctcc atggtgacag acgagcacat
     4321 cgccttcatc gccagcgtgc gcaatgcctt ctgccacaac cagtacccct tctacaagga
     4381 ggccctgcac gcccctatcc cactgttcac cgtggcccag cccaccacag aggagaagga
     4441 tggcctggga atcgcagagg ccctgctgaa ggtgctgagg gagtactgtg agatcgtgaa
     4501 gtcccagatc agtatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
     4561 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgcgcggcg agggcgaggg
     4621 cgatgccacc aacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
     4681 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc
     4741 cgaccacatg aagcgccacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     4801 gcgcaccatc agcttcaagg acgacggcac ctacaagacc cgcgccgagg tgaagttcga
     4861 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     4921 catcctgggg cacaagctgg agtacaactt caacagccac aacgtctata tcaccgccga
     4981 caagcagaag aacggcatca aggccaactt caagatccgc cacaacgtgg aggacggcag
     5041 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     5101 gcccgacaac cactacctga gcacccagtc caagctgagc aaagacccca acgagaagcg
     5161 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactcacg gcatggacga
     5221 gctgtacaag taccttcaac tgcctccact tgaaagactg acactgctag agcaacgcgt
     5281 gcagctaagc gactacaaag accatgacgg tgattataaa gatcatgaca tcgattacaa
     5341 ggatgacgat gacaagtaag gatccctccc ccccccctaa cgttactggc cgaagccgct
     5401 tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg
     5461 gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt
     5521 cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg
     5581 aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac
     5641 ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg
     5701 cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct
     5761 caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg
     5821 atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag
     5881 gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa ggatccaccg
     5941 gagcttacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc
     6001 agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc
     6061 gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc
     6121 gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc
     6181 acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag
     6241 ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg
     6301 cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag
     6361 ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc
     6421 gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc
     6481 gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc
     6541 ggtgcctgac gcccatcgat agatcctaat caacctctgg attacaaaat ttgtgaaaga
     6601 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     6661 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     6721 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     6781 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     6841 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     6901 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     6961 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     7021 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     7081 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     7141 tgggccgcct ccccgcctga gatcctttaa gaccaatgac ttacaaggca gctgtagatc
     7201 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     7261 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
     7321 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     7381 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     7441 agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat
     7501 aacttgcaaa gaaatgaata tcagagagtg agaggcccgg gttaattaag gaaagggcta
     7561 gatcattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     7621 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     7681 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     7741 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     7801 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     7861 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     7921 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     7981 tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc
     8041 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     8101 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     8161 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     8221 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     8281 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     8341 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     8401 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     8461 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     8521 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     8581 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     8641 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     8701 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     8761 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     8821 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     8881 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     8941 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     9001 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     9061 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     9121 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     9181 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     9241 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     9301 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     9361 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     9421 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     9481 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     9541 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     9601 cccgcgcgtt ggccgattca ttaatgcagc aagctcatgg ctgactaatt ttttttattt
     9661 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     9721 ttggaggcct aggcttttgc aaaaagctcc ccgtggcacg acaggtttcc cgactggaaa
     9781 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
     9841 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     9901 acaggaaaca gctatgacat gattacgaat ttcacaaata aagcattttt ttcactgcat
     9961 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat caactggata
    10021 actcaagcta accaaaatca tcccaaactt cccaccccat accctattac cactgccaat
    10081 tacctgtggt ttcatttact ctaaacctgt gattcctctg aattattttc attttaaaga
    10141 aattgtattt gttaaatatg tactacaaac ttagtagttg gaagggctaa ttcactccca
    10201 aagaagacaa gatatccttg atctgtggat ctaccacaca caaggctact tccctgatta
    10261 gcagaactac acaccagggc caggggtcag atatccactg acctttggat ggtgctacaa
    10321 gctagtacca gttgagccag ataaggtaga agaggccaat aaaggagaga acaccagctt
    10381 gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtgt tagagtggag
    10441 gtttgacagc cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa
    10501 gaactgctga tatcgagctt gctacaaggg actttccgct ggggactttc cagggaggcg
    10561 tggcctgggc gggactgggg agtggcgagc cctcagatcc tgcatataag cagctgcttt
    10621 ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
    10681 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
    10741 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
    10801 aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct
    10861 ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact
    10921 ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag
    10981 cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg
    11041 gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt
    11101 cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct
    11161 acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac
    11221 cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat
    11281 agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccggcc gctgatcttc
    11341 agacctggag gaggagatat gagggacaat tggagaagtg aattatataa atataaagta
    11401 gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga
    11461 gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc
    11521 actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata
    11581 gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc
    11641 acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag
    11701 gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg
    11761 ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg
    11821 atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa
    11881 tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt
    11941 ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata
    12001 gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt
    12061 aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac
    12121 aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta
    12181 gtgaacggat ctcgacggta tcgccgaatt cacaaatggc agtattcatc cacaatttta
    12241 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
    12301 cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt
    12361 attacaggga cagcagagat ccagtttgga ctagtcgtga
//
