LOCUS       sequence-34417-e         969 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Li M, Shin YH, Hou L, Huang X, Wei Z, Klann E, Zhang P
  TITLE     The adaptor protein of the anaphase promoting complex Cdh1 is 
            essential in maintaining replicative lifespan and in learning and 
            memory.
  JOURNAL   Nat Cell Biol. 2008 Sep . 10(9):1083-9.
  PUBMED    19160489
REFERENCE   2  (bases 1 to 969)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2008 Sep . 10(9):1083-9."
COMMENT     Sequence Label: Ets2DBM
FEATURES             Location/Qualifiers
     source          1..969
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             92..115
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             124..147
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             157..180
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca
       61 ctatagggag acccaagcta ccatggccat ggactataag gacgatgatg acaaaaagct
      121 atggactata aggacgatga tgacaaaaag ctaatggact ataaggacga tgatgacaaa
      181 aagcttggta ccgagctcgg atccatgaat gatttcggaa tcaagaatat ggaccaggta
      241 gcccctgtgg ctaacagtta cggaggtacc gtcaagcgcc agccagcctt tgacaccttt
      301 gatgggtccc tgtttgctgt ttttccttct ctaaatgaag agcaaacact gcaagaagtg
      361 ccaacaggct tggattccat ttctcatgac tccgccaact gtgaattgcc tttgttaacc
      421 ccgtgcagca aggctgtgat gagtcaagcc ttaaaagcta ccttcagtgg cttcaaaaag
      481 gaacagcggc gcctgggcat tccaaagaac ccctggctgt ggagtgagca acaggtatgc
      541 cagtggcttc tctgggccac caatgagttc agtctggtga acgtgaatct gcagaggttc
      601 ggcatgaatg gccagatgct gtgtaacctt ggcaaggaac gctttctgga gctggcacct
      661 gactttgtgg gtgacattct ctgggaacat ctggagcaaa tgatcaaaga aaaccaagaa
      721 aagacagaag atcaatatga agaaaattca cacctcacct ccgttcctca ttggattaac
      781 agcaatacat taggttttgg cacagagcag gcgccctatg gaatgcagac acagaattac
      841 cccaaaggcg gcctcctgga cagcatgtgt ccggcctcca cacccagcgt actcagctct
      901 gagcnnagtt tcagatgtcc ccaagtcntc ggctcagctc cgtcagcgtc acctactgct
      961 ctgtcagtc
//
