LOCUS       sequence-34426-e         674 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 674)
  AUTHORS   Bai J, Ramos RL, Ackman JB, Thomas AM, Lee RV, LoTurco JJ
  TITLE     RNAi reveals doublecortin is required for radial migration in rat 
            neocortex.
  JOURNAL   Nat Neurosci. 2003 Dec . 6(12):1277-83.
  PUBMED    14625554
REFERENCE   2  (bases 1 to 674)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2003 Dec . 6(12):1277-83."
COMMENT     Sequence Label: DCX 3UTRm3hp
FEATURES             Location/Qualifiers
     source          1..674
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    57..191
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(81..100)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     135..154
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(289..309)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(289..307)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(328..344)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(328..344)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(341..363)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    352..368
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(376..382,383..400,401..406))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    421..442
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(559..576)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 aggctataga cacaatagcc ttcttcgtga cttgagcttt ttctagataa tacgtagatc
       61 cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa
      121 aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca
      181 ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt
      241 gggaggtttt ttaattcttg cggccgcggc ggtacccagc ttttgttccc tttagtgagg
      301 gttaattgcg cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
      361 gctcacaatt ccacacaaca tacgagccgg gagcataaag tgtaaagcct ggggtgccta
      421 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
      481 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
      541 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
      601 agcggtatca gctcactcaa aggcggtaat acggttatcn acagaaatca ggggataacg
      661 caggaaagaa catg
//
