LOCUS       sequence-344642-        9857 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  6xHis_mT2Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) biosensor to measure 
            realtime changes in FRET reflecting fluctuations in cAMP levels in 
            living cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9857)
  TITLE     Jalink Lab Biosensor Plasmids
REFERENCE   2  (bases 1 to 9857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Epac-S-H127
FEATURES             Location/Qualifiers
     source          1..9857
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      5..14
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             23..40
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             join(53..55,56..58,59..571)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIE"
     CDS             2957..3691
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="MDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRD
                     HMVLLEFVTAAGITLGMDELYKGGSGGMVSKGEELFTGVVPILVELDGDVNGHKFSVSG
                     EGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPE
                     GYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
                     VYITADKQKNGIKANFKIRHNIE"
     CDS             join(3749..3751,3752..3754,3755..4465)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     promoter        complement(4517..4535)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4518..4535)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4555..4572)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4561..4785
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4831..5259
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4918..4937)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5128..5149
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5268..5288)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5273..5602
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5453..5588
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5515..5534
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5669..6463
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5723..5742)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6333..6352
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6637..6758
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6674..6693)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6728..6747
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6807..6823)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6807..6823)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6820..6842)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6831..6847
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6855..6861,6862..6879,6880..6885))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6900..6921
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7038..7055)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7209..7797)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7289..7308)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7968..8759,8760..8828))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8591..8610
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8829..8933)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9008..9027)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        9199..9578
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9579..9782
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9733..9753
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     9827..9846
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9827..9845
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tgccgccacc atggggggtt ctcatcatca tcatcatcat ggtgcggccg caatggtgag
       61 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
      121 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
      181 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
      241 caccctgtcc tggggcgtgc agtgcttcgc ccgctacccc gaccacatga agcagcacga
      301 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
      361 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
      421 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
      481 gtacaactac tttagcgaca acgtctatat caccgccgac aagcagaaga acggcatcaa
      541 ggccaacttc aagatccgcc acaacatcga ggacggcggc gtgcagctcg ccgaccacta
      601 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
      661 cacccagtcc aagctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
      721 gttcgtgacc gccgcccgat atcagcccgt gggaactcat gagatggagg aggagttggc
      781 cgaagctgtg gccctgctct cccagcgggg gcctgacgcc ctgctcactg tggcacttcg
      841 aaagccccca ggtcagcgca cggatgaaga gctggacctc atctttgagg agctgctgca
      901 catcaaggct gtggcccacc tctccaactc ggtgaagcga gaattagcgg ctgttctgct
      961 ctttgaacca cacagcaagg cagggaccgt gttgttcagc cagggggaca agggcacttc
     1021 gtggtacatt atctggaagg gatctgtcaa cgtggtgacc catggcaagg ggctggtgac
     1081 caccctgcat gagggagatg attttggaca gctggctctg gtgaatgatg caccccgggc
     1141 agccaccatc atcctgcgag aagacaactg tcatttcctg cgtgtggaca agcaggactt
     1201 caaccgtatc atcaaggatg tggaggcaaa gaccatgcgg ctggaagaac atggcaaagt
     1261 ggtgctggtg ctggagagag cctctcaggg cgccggccct tcccgacccc caaccccagg
     1321 caggaaccgg tatacagtga tgtctggcac cccagagaag atcctagagc ttctgttgga
     1381 ggccatggga ccagattcca gtgctcatga cccaacagag acattcctca gcgacttcct
     1441 cctgacccac agggtcttca tgcccagcgc ccaactctgc gctgcccttc tgcaccactt
     1501 ccatgtggag cctgcgggtg gcagcgagca ggagcgcagc acctacgtct gcaacaagag
     1561 gcagcagatc ttgcggctgg tcagccagtg ggtggccctg tatggctcca tgctccacac
     1621 tgaccctgtg gccaccagct tcctccagaa actctcagac ctggtgggca gggacacccg
     1681 actcagcaac ctgctgaggg agcagtggcc agagaggcgg cgatgccaca ggttggagaa
     1741 tggctgtggg aatgcatctc ctcagatgaa ggcccggaac ttgcctgttt ggctccccaa
     1801 ccaggacgag ccccttcctg gcagcagctg tgccatccaa gttggggata aagtccccta
     1861 tgacatctgc cggccagacc actcagtgtt gaccctgcag ctgcctgtga cagcctccgt
     1921 gagagaggtg atggcagcgt tggcccagga ggatggctgg accaaggggc aggtgctggt
     1981 gaaggtcaat tctgcaggtg atgccattgg cctgcagcca gatgcccgtg gtgtggccac
     2041 atctctgggg ctcaatgagc gtctctttgt tgtcaaccca caggaagcgc atgagctgat
     2101 cccacaccct gaccagctgg ggcccactgt gggctctgct gaggggctgg acctggtgag
     2161 tgccaaggac ctggcaggcc agctgacgga ccacgactgg agcctcttca acagtatcca
     2221 ccaggtggag ctgatccact atgtgctggg cccccagcat ctgcgggatg tcaccaccgc
     2281 caacctggag cgcttcatgc gccgcttcaa tgagctgcag tactgggtgg ccaccgagct
     2341 gtgtctctgc cccgtgcccg gcccccgggc ccagctgctc aggaagttca ttaagctggc
     2401 ggcccacctc aaggagcaga agaatctcaa ttccttcttt gccgtcatgt ttggcctcag
     2461 caactcggcc atcagccgcc tagcccacac ctgggagcgg ctgccccaca aagtccggaa
     2521 gctgtactcc gccctcgaga ggctgctgga tccctcatgg aaccaccggg tataccgact
     2581 ggccctcgcc aagctctccc ctcctgtcat ccccttcatg ccccttcttc tcaaagacat
     2641 ggccgccatt catgagggaa accacacact agtggagaat ctcatcaact ttgagaagat
     2701 gagaatgatg gccagagccg cgcggatgct gcaccactgc cgaagccaca accctgtgcc
     2761 tctctcacca ctcagaagcc gagtttccca cctccacgag gacagccagg tggcgaggat
     2821 ttccacatgc tcggagcagt ccctgagcac ccggagtcca gccagcacct gggcttatgt
     2881 ccagcagctg aaggtcattg acaaccagcg ggaactctcc cgcctctccc gagagctgga
     2941 gccagctagc gagctcatgg acggcggcgt gcagctcgcc gaccactacc agcagaacac
     3001 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagct accagtccgc
     3061 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     3121 cgccgggatc actctcggca tggacgagct gtacaagggt ggcagcggtg gcatggtgag
     3181 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
     3241 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
     3301 gaccctgaag ctgatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
     3361 caccctgggc tacggcctgc agtgcttcgc ccgctacccc gaccacatga agcagcacga
     3421 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
     3481 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
     3541 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
     3601 gtacaactac aacagccaca acgtctatat caccgccgac aagcagaaga acggcatcaa
     3661 ggccaacttc aagatccgcc acaacatcga gccgaatttt gtcttcctga tcggcgccgc
     3721 aggaatactc ttcgtatcta gcgagctcat ggtgagcaag ggcgaggagc tgttcaccgg
     3781 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
     3841 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagctga tctgcaccac
     3901 cggcaagctg cccgtgccct ggcccaccct cgtgaccact ctgggctacg gcctacagtg
     3961 cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
     4021 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
     4081 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
     4141 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt
     4201 ctatatcacc gccgacaagc agaagaacgg catcaaggcc aacttcaaga tccgccacaa
     4261 catcgaggac ggcggcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
     4321 cggccccgtg ctgctgcccg acaaccacta cctgagctac cagtccgccc tgagcaaaga
     4381 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
     4441 tctcggcatg gacgagctgt acaagccgaa ttccctcgag gttaacgcgg cgcgcctcta
     4501 atctagaggg ccctattcta tagtgtcacc taaatgctag agctcgctga tcagcctcga
     4561 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
     4621 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
     4681 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
     4741 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa
     4801 gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg
     4861 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     4921 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     4981 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     5041 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     5101 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     5161 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     5221 taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca
     5281 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     5341 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     5401 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     5461 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     5521 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt
     5581 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga
     5641 tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc
     5701 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg
     5761 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac
     5821 cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc
     5881 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg
     5941 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga
     6001 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg
     6061 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
     6121 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt
     6181 cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc
     6241 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
     6301 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
     6361 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc
     6421 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc
     6481 gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc
     6541 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
     6601 cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat
     6661 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     6721 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc
     6781 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     6841 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     6901 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     6961 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     7021 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     7081 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     7141 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     7201 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     7261 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     7321 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     7381 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     7441 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     7501 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     7561 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     7621 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     7681 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     7741 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     7801 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     7861 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     7921 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     7981 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     8041 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     8101 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     8161 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     8221 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     8281 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     8341 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     8401 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     8461 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     8521 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     8581 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     8641 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     8701 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     8761 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     8821 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     8881 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     8941 ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc ctatggtgca
     9001 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg
     9061 tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga
     9121 ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac
     9181 gggccagata tacgcgttga cattgattat tgactagtta ttaatagtaa tcaattacgg
     9241 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
     9301 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
     9361 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
     9421 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
     9481 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
     9541 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
     9601 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
     9661 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
     9721 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
     9781 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
     9841 atagggagac ccaagct
//
