LOCUS       sequence-345001-        9785 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cre-inducible lentiviral vector expressing hM3Dq.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9785)
  AUTHORS   Klawonn AM, Fritz M, Castany S, Pignatelli M, Canal C, Simila F, 
            Tejeda HA, Levinsson J, Jaarola M, Jakobsson J, Hidalgo J, Heilig M,
            Bonci A, Engblom D
  TITLE     Microglial activation elicits a negative affective state through 
            prostaglandin-mediated modulation of striatal neurons.
  JOURNAL   Immunity. 2021 Feb 9;54(2):225-234.e6. doi: 
            10.1016/j.immuni.2020.12.016. Epub 2021 Jan 20.
  PUBMED    33476547
REFERENCE   2  (bases 1 to 9785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.immuni.2020.12.016"; journalName: "Immunity"; date: 
            "2021-02-9- 9"; volume: "54"; issue: "2"; pages: "225-234.e6"
COMMENT     Sequence Label: pLV.CMV.flex.hM3Dq.Cherry
FEATURES             Location/Qualifiers
     source          1..9785
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        26..130
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(131..199,200..991)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(349..368)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1162..1750
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1651..1670
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1904..1921
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2038..2059
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2074..2079,2080..2097,2098..2104)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2112..2128
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2117..2139
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2136..2152
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2136..2152
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2171..2191
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2173..2191
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        2292..2671
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2672..2875
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             2890..3070
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3117..3242
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3735..3968
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4153..4197
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        4780..4983
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    5068..5101
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    5164..5197
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(5210..5920)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5488..5507)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     5757..5775
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      5917..5926
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(7730..7763)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(7834..7867)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(7944..7964)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8362..8373)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8522..8755
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8827..8948
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8864..8883)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8918..8937
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8988..9123
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9143..9162)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9144..9162)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9172..9189)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9172..9188)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9181..9203)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9330..9758
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9417..9436)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9627..9648
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
       61 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
      121 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
      181 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
      241 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
      301 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
      361 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
      421 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
      481 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
      541 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
      601 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
      661 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
      721 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
      781 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
      841 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
      901 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
      961 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     1021 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     1081 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     1141 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     1201 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     1261 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     1321 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     1381 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     1441 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     1501 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     1561 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     1621 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     1681 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     1741 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     1801 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     1861 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     1921 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     1981 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     2041 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     2101 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     2161 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttggcc
     2221 attgcatacg ttgtatccat atcataatat gtacatttat attggctcat gtccaacatt
     2281 accgccatgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
     2341 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
     2401 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
     2461 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
     2521 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
     2581 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
     2641 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
     2701 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
     2761 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
     2821 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctcgttt
     2881 agtgaaccgg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     2941 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     3001 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     3061 atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct
     3121 ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg
     3181 tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg
     3241 tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg
     3301 gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg
     3361 cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac
     3421 aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc
     3481 tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag
     3541 aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct
     3601 ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa
     3661 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa
     3721 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg
     3781 ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag
     3841 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc
     3901 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa
     3961 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg
     4021 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag
     4081 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa
     4141 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg
     4201 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga
     4261 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag
     4321 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc
     4381 gaaggaatag aagaagrmrr yrgasakasa racwrarrma kwwcmawwms awwwkwsaam
     4441 rrwtcwmray kktatcgrya asmwtkrrwr kwcygsrtkr mrkmrswtam rgtwrmwggm
     4501 ccrcytsksy gmccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
     4561 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
     4621 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
     4681 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
     4741 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
     4801 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
     4861 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
     4921 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga
     4981 gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat
     5041 agaagacacc gactctagag gatccccata acttcgtata aagtatccta tacgaagtta
     5101 tatcaaaata ggaagaccaa tgcttcacca tcgacccgaa ttgccaagca tcaccatcga
     5161 cccataactt cgtataatgt atgctatacg aagttattgt acaaccggtt tacttgtaca
     5221 gctcgtccat gccgccggtg gagtggcggc cctcggcgcg ttcgtactgt tccacgatgg
     5281 tgtagtcctc gttgtgggag gtgatgtcca acttgatgtt gacgttgtag gcgccgggca
     5341 gctgcacggg cttcttggcc ttgtaggtgg tcttgacctc agcgtcgtag tggccgccgt
     5401 ccttcagctt cagcctctgc ttgatctcgc ccttcagggc gccgtcctcg gggtacatcc
     5461 gctcggagga ggcctcccag cccatggtct tcttctgcat tacggggccg tcggagggga
     5521 agttggtgcc gcgcagcttc accttgtaga tgaactcgcc gtcctgcagg gaggagtcct
     5581 gggtcacggt caccacgccg ccgtcctcga agttcatcac gcgctcccac ttgaagccct
     5641 cggggaagga cagcttcaag tagtcgggga tgtcggcggg gtgcttcacg taggccttgg
     5701 agccgtacat gaactgaggg gacaggatgt cccaggcgaa gggcaggggg ccacccttgg
     5761 tcaccttcag cttggcggtc tgggtgccct cgtaggggcg gccctcgccc tcgccctcga
     5821 tctcgaactc gtggccgttc acggagccct ccatgtgcac cttgaagcgc atgaactcct
     5881 tgatgatggc catgttatcc tcctcgccct tgctcaccat ggtggcgacc gggggatcct
     5941 tcaaggcctg ctcgggtgcg cgcttgtgaa aaatgaccga ctgtctctgc tggtactgct
     6001 gcttgcgcct cttttttttg tcacactggc acagcagcag catcttgaaa gtggttctga
     6061 atgttttgtt gcacagagca tagcacacgg ggttcacggt gctgttgatg tagcacagcc
     6121 agtagcccag attccaaaag gttttgggta tgcagctgtc acaaaaggtg ttcaccagaa
     6181 ccatgatgtt gtatggggtc caagtgatga tgaaggcaag caagatcgca ctgagggtct
     6241 gggccgcttt cttctccttg accagggaca tccttttccg cttagtgatc tgacttctgg
     6301 tcttcagagc aaacctcttg gccagagtgg cttccttgaa ggacagaggt agagtggccg
     6361 tgctcttacc cactgaggag ttgacgtcag aagtcttagc tgtgtccacg gctgactcta
     6421 gctggatggg aagcttggag aagctttttg gaaaactgcc tccatcgtcc acgctcttct
     6481 gggcctgcag cttgtcggct ttcctctcca agtccaccat ccccagctcc tcctcaggca
     6541 cctgcaggtt gtccgatgag ggtaacttgg tggagttgag gatggtgctg tgacccggaa
     6601 gcttgagcac gatggagtag atggctctcg tctcggagcc aatgtcctcc tcgtcggagg
     6661 aggcggagtt ctccagggag gcagcagcat cattgttgtt ccaactgtca ctgctgctgt
     6721 ggtcttggtc catctgctcg gagctgggtt tccagctctt ggttgtgaac cagaagtggc
     6781 agcggccata cttcctcctg ttggagcgtt tcatgctttg ctgttgaagt tcgtaactgc
     6841 tgcagcttcg agaactgccc gtggggtgga caaagttttc tgtctctgcc tctgtcccag
     6901 aggcttgcag gccagcaagc tctttggtac gcttttcagt ttccttatag atcctccagt
     6961 ataaaatagt cataatggtg acaggcatat aaaaaccagc gatggctgtg ccaaaagtaa
     7021 tggtgggctc actgaggaac tgaatgaagc actctcccgg aggcacagtt ctctttccaa
     7081 caaagtattg ccagaacaag atggcaggag cccaaaggac aaaggagatg acccaagcca
     7141 gaccgatcat cacaccggct ctctttgttg ttcgtttggc tcggtacgtg agcggcctcg
     7201 tgatggaaaa gtatctgtca aagctgatga ccagaagatt cataacagag gcattgctgg
     7261 ctacgcagtc aatggcaagc cagaggtcac aggccaagtt ccctaaggcc catcgattca
     7321 tgatgatgta ggtcgtaaac agattcattg aaatgacccc gataatcaga tcggcacagg
     7381 ccaggcttaa gaggaagtag ttgttgaccg tcttcagctg cttgttgacc ttaaatgaca
     7441 caattaccag gatgttgccg atgatggtca ccaaggccag gatgcccgtt aagaaagcga
     7501 tgaagaccac ttgccagacg gtatgacctc ccagagggtc atcggtggta ccgtctggag
     7561 aggagaaatt gccagctgct cgagaaacat tgtagctgcc gaaatgagtg acggttcccg
     7621 ggggcagccc tgcatcggag gggctgtgta tccaggagga gctgatgttt ggaaacaaag
     7681 gcgaggttgt actgttattg tgcaaggtca tggtggcgct agcaccggta taacttcgta
     7741 taggatactt tatacgaagt tatcattggg attcttccta ttttgatcca agcatcacca
     7801 tcgaccctct agtccagatc tcaccatcga cccataactt cgtatagcat acattatacg
     7861 aagttatgtc cctcgaagag gttcgtcgac aatcaacctc tggattacaa aatttgtgaa
     7921 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     7981 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     8041 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     8101 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     8161 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     8221 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     8281 gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg
     8341 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     8401 ctgccggctc tagagcctct tccgcgtctt cgccttcccg ggtcgagctc ggtaccttta
     8461 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     8521 ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct
     8581 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     8641 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     8701 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt
     8761 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
     8821 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     8881 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     8941 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa
     9001 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     9061 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     9121 gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt
     9181 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     9241 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     9301 acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc
     9361 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     9421 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     9481 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     9541 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     9601 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     9661 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     9721 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac
     9781 aattt
//
