LOCUS       sequence-34506-e         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  TITLE     SGC Q3
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: UHRF1
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             34..51
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             64..84
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             594..611
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(664..682)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      678..725
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatatacc atgcatcatc atcatcatca cagcagcggc
       61 agagaaaact tgtatttcca gggcatgtgg gatgagacgg aattggggct gtacaaggtc
      121 aatgagtacg tcgatgctcg ggacacgaac atgggggcgt ggtttgaggc gcaggtggtc
      181 agggtgacgc ggaaggcccc ctcccgggac gagccctgca gctccacgtc caggccggcg
      241 ctggaggagg acgtcattta ccacgtgaaa tacgacgact acccggagaa cggcgtggtc
      301 cagatgaact ccagggacgt ccgagcgcgc gcccgcacca tcatcaagtg gcaggacctg
      361 gaggtgggcc aggtggtcat gctcaactac aaccccgaca accccaagga gcggggcttc
      421 tggtacgacg cggagatctc caggaagcgc gagaccagga cggcgcggga actctacgcc
      481 aacgtggtgc tgggggatga ttctctgaac gactgtcgga tcatcttcgt ggacgaagtc
      541 ttcaagattg agcggccggg tgnnngatga cgaagcttgc ggccgcactc gagcaccacc
      601 accaccacca ctgagatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg
      661 ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt
      721 ttttgctgaa aggaggaact atatccggat tggcgaatgg gacgcgccct gtagcggcgc
      781 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacac
//
