LOCUS       sequence-34508-e         825 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 825)
  TITLE     SGC Q3
REFERENCE   2  (bases 1 to 825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: EPHB3
FEATURES             Location/Qualifiers
     source          1..825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     81..143
                     /codon_start=1
                     /product="signal sequence from honeybee melittin"
                     /label=melittin signal sequence
                     /translation="MKFLVNVALVFMVVYISYIYA"
     CDS             156..173
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             195..215
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 gttttgtaat aaaaaaacct ataaatattc cggattattc ataccgtccc accatcgggc
       61 gcggatctcg gtccgaaacc atgaaattct tagtcaacgt tgcccttgtt tttatggtcg
      121 tatacatttc ttacatctat gcggccgctc cggaacatca ccatcaccat cacgattacg
      181 atatcccaac gaccgaaaac ctgtattttc agggcgccat ggatgaagag accctcatgg
      241 acacaaaatg ggtaacatct gagttggcgt ggacatctca tccagaaagt gggtgggaag
      301 aggtgagtgg ctacgatgag gccatgaatc ccatccgcac ataccaggtg tgtaatgtgc
      361 gcgagtcaag ccagaacaac tggcttcgca cggggttcat ctggcggcgg gatgtgcagc
      421 gggtctacgt ggagctcaag ttcactgtgc gtgactgcaa cagcatcccc aacatccccg
      481 gctcctgcaa ggagaccttc aacctcttct actacgaggc tgacagcgat gtggcctcag
      541 cctcctcccc cttctggatg gagaacccct acgtgaaagt ggacaccatt gcacccgatg
      601 agagcttctc gcggctggat gccggccgtg tcaacaccaa ggtgcgcagc tttgggccac
      661 tttccaaggc tggcttctac ctggccttcc aggaccaggg cgcctgcatg tcgctcatct
      721 ccgtgcgcgc cttctacaag aagtgatgaa gcttgtcgag aagtactaga ggatcataat
      781 cagccatacc acatttgtag aggttttact tgctttaaaa acctc
//
