LOCUS       sequence-34509-e         831 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  TITLE     SGC Q3
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: GIMAP2
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             5..27
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             37..54
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             67..87
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             741..758
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(811..829)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 taattttgtt taactttaag aaggagatat accatgcatc atcatcatca tcacagcagc
       61 ggcagagaaa acttgtattt ccagggcagc agatctgagc tgagaatcat cctggtgggc
      121 aaaacaggaa ctggcaaaag tgctgcaggg aacagcatcc tcaggaagca agcatttgaa
      181 tcgaagctgg gttcccagac cttgactaag acttgcagca aaagtcaggg aagctgggga
      241 aatagagaga ttgtcattat tgacacacca gatatgtttt cttggaagga ccactgtgaa
      301 gctctgtaca aagaggtgca gaggtgctac ttgctgtctg caccaggacc ccatgtgctg
      361 ctcctggtga ctcagctggg ccgctatacc tcacaggacc agcaggctgc acagagggtg
      421 aaggagatct ttggagagga tgccatggga cacacaattg tcctctttac ccacaaggaa
      481 gacctcaatg gtggctccct gatggattac atgcacgact cagataacaa agccctaagc
      541 aagctggtgg cagcatgtgg tgggcgaatc tgtgccttta ataaccgtgc tgaagggagc
      601 aatcaggatg accaagtgaa ggaactaatg gactgtattg aggatctgtt gatggagaaa
      661 aatggtgatc actataccaa tgggttgtac agcctaatac agaggtctaa atgatgacga
      721 agcttgcggc cgcactcgag caccaccacc accaccactg agatccggct gctaacaaag
      781 cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca t
//
