LOCUS       sequence-345139-       10451 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mLAMA4 (VGFNFRTL) antigen as a fusion to luciferase and 
            Cre recombinase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10451)
  AUTHORS   Burger ML, Cruz AM, Crossland GE, Gaglia G, Ritch CC, Blatt SE, 
            Bhutkar A, Canner D, Kienka T, Tavana SZ, Barandiaran AL, Garmilla 
            A, Schenkel JM, Hillman M, de Los Rios Kobara I, Li A, Jaeger AM, 
            Hwang WL, Westcott PMK, Manos MP, Holovatska MM, Hodi FS, Regev A, 
            Santagata S, Jacks T
  TITLE     Antigen dominance hierarchies shape TCF1(+) progenitor CD8 T cell 
            phenotypes in tumors.
  JOURNAL   Cell. 2021 Sep 16;184(19):4996-5014.e26. doi: 
            10.1016/j.cell.2021.08.020.
  PUBMED    34534464
REFERENCE   2  (bases 1 to 10451)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2021.08"; journalName: "Cell"; date: "2021-09-16- 
            16"; volume: "184"; issue: "19"; pages: "4996-5014.e26"
COMMENT     Sequence Label: Lenti-LucL
FEATURES             Location/Qualifiers
     source          1..10451
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     133..152
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             231..251
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             252..1280
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="SNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLL
                     SVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLP
                     RPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFL
                     GIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVER
                     WISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQR
                     YLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLED
                     GD"
     misc_feature    1333..1921
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1386..1406)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1804..1815)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(1924..1940)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1925..1941)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             2450..2630
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(2692..3280)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2772..2791)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3451..4242,4243..4311))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4074..4093
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4312..4416)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4491..4510)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        4682..5061
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5063..5261
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5216..5236
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             5279..5459
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5506..5631
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6128..6361
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6546..6590
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6888..7005
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7146..8357
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     8194..8213
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             8404..10053
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(8470..8488)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     promoter        join(10152..10451,1..200)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(10187..10210)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagagg ctgggaaggg
       61 gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct
      121 ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt
      181 cctcatctcc gggagacccg acattagcgc tacagcttaa gcggaccatg cccaagaaga
      241 agaggaaggt gtccaattta ctgaccgtac accaaaattt gcctgcatta ccggtcgatg
      301 caacgagtga tgaggttcgc aagaacctga tggacatgtt cagggatcgc caggcgtttt
      361 ctgagcatac ctggaaaatg cttctgtccg tttgccggtc gtgggcggca tggtgcaagt
      421 tgaataaccg gaaatggttt cccgcagaac ctgaagatgt tcgcgattat cttctatatc
      481 ttcaggcgcg cggtctggca gtaaaaacta tccagcaaca tttgggccag ctaaacatgc
      541 ttcatcgtcg gtccgggctg ccacgaccaa gtgacagcaa tgctgtttca ctggttatgc
      601 ggcggatccg aaaagaaaac gttgatgccg gtgaacgtgc aaaacaggct ctagcgttcg
      661 aacgcactga tttcgaccag gttcgttcac tcatggaaaa tagcgatcgc tgccaggata
      721 tacgtaatct ggcatttctg gggattgctt ataacaccct gttacgtata gccgaaattg
      781 ccaggatcag ggttaaagat atctcacgta ctgacggtgg gagaatgtta atccatattg
      841 gcagaacgaa aacgctggtt agcaccgcag gtgtagagaa ggcacttagc ctgggggtaa
      901 ctaaactggt cgagcgatgg atttccgtct ctggtgtagc tgatgatccg aataactacc
      961 tgttttgccg ggtcagaaaa aatggtgttg ccgcgccatc tgccaccagc cagctatcaa
     1021 ctcgcgccct ggaagggatt tttgaagcaa ctcatcgatt gatttacggc gctaaggatg
     1081 actctggtca gagatacctg gcctggtctg gacacagtgc ccgtgtcgga gccgcgcgag
     1141 atatggcccg cgctggagtt tcaataccgg agatcatgca agctggtggc tggaccaatg
     1201 taaatattgt catgaactat atccgtaacc tggatagtga aacaggggca atggtgcgcc
     1261 tgctggaaga tggcgattag aaacgttgtt gtttggggtt gaattactct tctagaccgc
     1321 ggcgcgcccg ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     1381 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     1441 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     1501 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     1561 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     1621 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     1681 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
     1741 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     1801 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     1861 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     1921 catcgatacc gtcgacctcg atcgagacct agaaaaacat ggagcaatca caagtagcaa
     1981 tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg
     2041 ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct
     2101 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca
     2161 agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt ggcagaacta
     2221 cacaccaggg ccagggatca gatatccact gacctttgga tggtgctaca agctagtacc
     2281 agttgagcaa gagaaggtag aagaagccaa tgaaggagag aacacccgct tgttacaccc
     2341 tgtgagcctg catgggatgg atgacccgga gagagaagta ttagagtgga ggtttgacag
     2401 ccgcctagca tttcatcaca tggcccgaga gctgcatccg gactgtactg ggtctctctg
     2461 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     2521 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     2581 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gcatgtgagc
     2641 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     2701 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     2761 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     2821 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     2881 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     2941 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     3001 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     3061 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     3121 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     3181 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     3241 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     3301 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     3361 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     3421 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     3481 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     3541 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     3601 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     3661 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     3721 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     3781 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     3841 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     3901 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     3961 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     4021 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     4081 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     4141 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     4201 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     4261 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     4321 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     4381 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     4441 tgacgtcgac ggatcgggag atctcccgat cccctatggt gcactctcag tacaatctgc
     4501 tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag
     4561 tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag
     4621 aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgt
     4681 tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc
     4741 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
     4801 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
     4861 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
     4921 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
     4981 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
     5041 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
     5101 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
     5161 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
     5221 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gcgcgttttg cctgtactgg
     5281 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     5341 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     5401 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     5461 tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg
     5521 actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca
     5581 aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag
     5641 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
     5701 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
     5761 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
     5821 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
     5881 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
     5941 aacaaaagta agaccaccgc acagcaagcg gccggccgct gatcttcaga cctggaggag
     6001 gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac
     6061 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag
     6121 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag
     6181 cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga
     6241 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca
     6301 tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc
     6361 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta
     6421 gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca
     6481 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc
     6541 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt
     6601 ttaacataac aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg
     6661 taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt
     6721 caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa
     6781 tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg aacggatcgg
     6841 cactgcgtgc gccaattctg cagacaaatg gcagtattca tccacaattt taaaagaaaa
     6901 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
     6961 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg
     7021 gacagcagag atccagtttg gttagtaccg ggcccgggtc gactagtgat cagtgtgagg
     7081 gagtgtaaag ctggtttcag agatccagtt tggttagtac cgggcccgct ctagacggtt
     7141 gatctggcct ccgcgccggg ttttggcgcc tcccgcgggc gcccccctcc tcacggcgag
     7201 cgctgccacg tcagacgaag ggcgcagcga gcgtcctgat ccttccgccc ggacgctcag
     7261 gacagcggcc cgctgctcat aagactcggc cttagaaccc cagtatcagc agaaggacat
     7321 tttaggacgg gacttgggtg actctagggc actggttttc tttccagaga gcggaacagg
     7381 cgaggaaaag tagtcccttc tcggcgattc tgcggaggga tctccgtggg gcggtgaacg
     7441 ccgatgatta tataaggacg cgccgggtgt ggcacagcta gttccgtcgc agccgggatt
     7501 tgggtcgcgg ttcttgtttg tggatcgctg tgatcgtcac ttggtgagta gcgggctgct
     7561 gggctggccg gggctttcgt ggccgccggg ccgctcggtg ggacggaagc gtgtggagag
     7621 accgccaagg gctgtagtct gggtccgcga gcaaggttgc cctgaactgg gggttggggg
     7681 gagcgcagca aaatggcggc tgttcccgag tcttgaatgg aagacgcttg tgaggcgggc
     7741 tgtgaggtcg ttgaaacaag gtggggggca tggtgggcgg caagaaccca aggtcttgag
     7801 gccttcgcta atgcgggaaa gctcttattc gggtgagatg ggctggggca ccatctgggg
     7861 accctgacgt gaagtttgtc actgactgga gaactcggtt tgtcgtctgt tgcgggggcg
     7921 gcagttatgg cggtgccgtt gggcagtgca cccgtacctt tgggagcgcg cgccctcgtc
     7981 gtgtcgtgac gtcacccgtt ctgttggctt ataatgcagg gtggggccac ctgccggtag
     8041 gtgtgcggta ggcttttctc cgtcgcagga cgcagggttc gggcctaggg taggctctcc
     8101 tgaatcgaca ggcgccggac ctctggtgag gggagggata agtgaggcgt cagtttcttt
     8161 ggtcggtttt atgtacctat cttcttaagt agctgaagct ccggttttga actatgcgct
     8221 cggggttggc gagtgtgttt tgtgaagttt tttaggcacc ttttgaaatg taatcatttg
     8281 ggtcaatatg taattttcag tgttagacta gtaaattgtc cgctaaattc tggccgtttt
     8341 tggctttttt gttagacgaa gctaacgcgc tagctaactc gaacgctagc tgtgcgatcg
     8401 tttatggaag acgccaaaaa cataaagaaa ggcccggcgc cattctatcc gctggaagat
     8461 ggaaccgctg gagagcaact gcataaggct atgaagagat acgccctggt tcctggaaca
     8521 attgctttta cagatgcaca tatcgaggtg gacatcactt acgctgagta cttcgaaatg
     8581 tccgttcggt tggcagaagc tatgaaacga tatgggctga atacaaatca cagaatcgtc
     8641 gtatgcagtg aaaactctct tcaattcttt atgccggtgt tgggcgcgtt atttatcgga
     8701 gttgcagttg cgcccgcgaa cgacatttat aatgaacgtg aattgctcaa cagtatgggc
     8761 atttcgcagc ctaccgtggt gttcgtttcc aaaaaggggt tgcaaaaaat tttgaacgtg
     8821 caaaaaaagc tcccaatcat ccaaaaaatt attatcatgg attctaaaac ggattaccag
     8881 ggatttcagt cgatgtacac gttcgtcaca tctcatctac ctcccggttt taatgaatac
     8941 gattttgtgc cagagtcctt cgatagggac aagacaattg cactgatcat gaactcctct
     9001 ggatctactg gtctgcctaa aggtgtcgct ctgcctcata gaactgcctg cgtgagattc
     9061 tcgcatgcca gagatcctat ttttggcaat caaatcattc cggatactgc gattttaagt
     9121 gttgttccat tccatcacgg ttttggaatg tttactacac tcggatattt gatatgtgga
     9181 tttcgagtcg tcttaatgta tagatttgaa gaagagctgt ttctgaggag ccttcaggat
     9241 tacaagattc aaagtgcgct gctggtgcca accctattct ccttcttcgc caaaagcact
     9301 ctgattgaca aatacgattt atctaattta cacgaaattg cttctggtgg cgctcccctc
     9361 tctaaggaag tcggggaagc ggttgccaag aggttccatc tgccaggtat caggcaagga
     9421 tatgggctca ctgagactac atcagctatt ctgattacac ccgaggggga tgataaaccg
     9481 ggcgcggtcg gtaaagttgt tccatttttt gaagcgaagg ttgtggatct ggataccggg
     9541 aaaacgctgg gcgttaatca aagaggcgaa ctgtgtgtga gaggtcctat gattatgtcc
     9601 ggttatgtaa acaatccgga agcgaccaac gccttgattg acaaggatgg atggctacat
     9661 tctggagaca tagcttactg ggacgaagac gaacacttct tcatcgttga ccgcctgaag
     9721 tctctgatta agtacaaagg ctatcaggtg gctcccgctg aattggaatc catcttgctc
     9781 caacacccca acatcttcga cgcaggtgtc gcaggtcttc ccgacgatga cgccggtgaa
     9841 cttcccgccg ccgttgttgt tttggagcac ggaaagacga tgacggaaaa agagatcgtg
     9901 gattacgtcg ccagtcaagt aacaaccgcg aaaaagttgc gcggaggagt tgtgtttgtg
     9961 gacgaagtac cgaaaggtct taccggaaaa ctcgacgcaa gaaaaatcag agagatcctc
    10021 ataaaggcca agaagggcgg aaagatcgcc gtgtttgatg gctttgaagt aggcttcaat
    10081 ttccgaacat tacagccaaa tgggttacta taaaaaccgc tgttcctagg aatcccgagg
    10141 cctattctac cgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc
    10201 agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc
    10261 caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc
    10321 tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat
    10381 ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg
    10441 ggtaggcctt t
//
