LOCUS       sequence-34514-e         749 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral transfer plasmid for Tet-regulated shRNA expression with 
            a minimal CMV promoter. Contains DsRed2 and Venus fluorescent 
            reporters..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Zuber J, McJunkin K, Fellmann C, Dow LE, Taylor MJ, Hannon GJ, Lowe 
            SW
  TITLE     Toolkit for evaluating genes required for proliferation and survival
            using tetracycline-regulated RNAi.
  JOURNAL   Nat Biotechnol. 2011 Jan . 29(1):79-83.
  PUBMED    21131983
REFERENCE   2  (bases 1 to 749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2011 Jan . 29(1):79-83."
COMMENT     Sequence Label: TRMPV-ns
FEATURES             Location/Qualifiers
     source          1..749
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(232..256)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(253..291)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    join(324..342,343..365,366..384,385..407,408..426,427..449,
                     450..468,469..491,492..510,511..533,534..552,553..575,
                     576..594)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     primer_bind     complement(660..676)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(692..714)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
ORIGIN
        1 cgcccttggt caccttcagc ttcacggtgt tgtggccctc gtaggggcgg ccctcgccct
       61 cgccctcgat ctcgaactcg tggccgttca cggtgccctc catgcgcacc ttgaagcgca
      121 tgaactcggt gatgacgttc tcggaggagg ccatggtgga tccgtcgaga ggctggatcg
      181 gtcccggtgt cttctatgga ggtcaaaaca gcgtggatgg cgtctccagg cgatctgacg
      241 gttcactaaa cgagctctgc ttatataggc ctcccaccgt acacgcctac ctcgacccgg
      301 gtaccgagct cgactttcac ttttctctat cactgatagg gagtggtaaa ctcgactttc
      361 acttttctct atcactgata gggagtggta aactcgactt tcacttttct ctatcactga
      421 tagggagtgg taaactcgac tttcactttt ctctatcact gatagggagt ggtaaactcg
      481 actttcactt ttctctatca ctgataggga gtggtaaact cgactttcac ttttctctat
      541 cactgatagg gagtggtaaa ctcgactttc acttttctct atcactgata gggagtggta
      601 aactcgatgc tctagaggat cgatccccca gatcttcaat tccggcgcct agagaaggag
      661 tgagggctgg ataaagggag gatcgaggcg gggtcgaacg aggaggttca agggggagag
      721 acggggcgga tggaggaaga ggaggcgga
//
