LOCUS       sequence-34517-e         749 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral transfer plasmid for Tet-regulated shRNA expression with 
            a minimal CMV promoter. Contains DsRed2 and Venus fluorescent 
            reporters..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Zuber J, McJunkin K, Fellmann C, Dow LE, Taylor MJ, Hannon GJ, Lowe 
            SW
  TITLE     Toolkit for evaluating genes required for proliferation and survival
            using tetracycline-regulated RNAi.
  JOURNAL   Nat Biotechnol. 2011 Jan . 29(1):79-83.
  PUBMED    21131983
REFERENCE   2  (bases 1 to 749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2011 Jan . 29(1):79-83."
COMMENT     Sequence Label: TRMPV-Hygro
FEATURES             Location/Qualifiers
     source          1..749
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(231..255)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(252..290)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    join(323..341,342..364,365..383,384..406,407..425,426..448,
                     449..467,468..490,491..509,510..532,533..551,552..574,
                     575..593)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     primer_bind     complement(659..675)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(691..713)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
ORIGIN
        1 gcccttggtc accttcagct tcacggtgtt gtggccctcg taggggcggc cctcnccctc
       61 gccctcgatc tcgaactcgt ggccgttcac ggtgccctcc atgcgcacct tgaagcgcat
      121 gaactcggtg atgacgttct cggaggaggc catggtggat ccgtcgagag gctggatcgg
      181 tcccggtgtc ttctatggag gtcaaaacag cgtggatggc gtctccaggc gatctgacgg
      241 ttcactaaac gagctctgct tatataggcc tcccaccgta cacgcctacc tcgacccggg
      301 taccgagctc gactttcact tttctctatc actgataggg agtggtaaac tcgactttca
      361 cttttctcta tcactgatag ggagtggtaa actcgacttt cacttttctc tatcactgat
      421 agggagtggt aaactcgact ttcacttttc tctatcactg atagggagtg gtaaactcga
      481 ctttcacttt tctctatcac tgatagggag tggtaaactc gactttcact tttctctatc
      541 actgataggg agtggtaaac tcgactttca cttttctcta tcactgatag ggagtggtaa
      601 actcgatgct ctagaggatc gatcccccag atcttcaatt ccggcgccta gagaaggagt
      661 gagggctgga taaagggagg atcgaggcgg ggtcgaacga ggaggttcaa gggggagaga
      721 cggggcggat ggaggaagag gaggcggag
//
