LOCUS       sequence-345388-        3777 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3777)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 3777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC35F1_STOP
FEATURES             Location/Qualifiers
     source          1..3777
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1334..1433)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1450..1469)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1451..1469)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1474..1490)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1474..1490)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1592..1611)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1603..2412
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1670..1689)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2559..3147
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3048..3067
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3301..3318
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3477..3504)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3596..3682)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3731..3753
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3745..3762
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3746..3762
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatgat cccccctgag
      121 cagcctcagc agcagctgca gccaccatct ccagcacctc caaaccacgt ggtgaccaca
      181 atcgagaatc tgcctgcaga gggatccgga ggaggaggat ccctgtctgc cagctccagg
      241 gcaggcgtgc gccagaggat cagaaaggtg ctgaaccgcg agatgctgat ctctgtggcc
      301 ctgggacagg tgctgagcct gctgatctgc ggcatcggcc tgacatctaa gtacctgagc
      361 gaggactttc acgccaacac cccagtgttc cagagctttc tgaattacat cctgctgttc
      421 ctggtgtata ccacaaccct ggccgtgagg cagggagagg agaacctgct ggccatcctg
      481 cggagaaggt ggtggaagta catgatcctg ggcctgatcg acctggaggc caattatctg
      541 gtggtgaagg cctaccagta tacaaccctg acatccatcc agctgctgga ttgttttgtg
      601 atccccgtgg tcatcctgct gtcttggttc tttctgctga tccggtataa ggccgtgcac
      661 ttcatcggca tcgtggtgtg catcctgggc atgggctgta tggtgggcgc cgacgtgctg
      721 gtgggcagac accagggagc aggagagaac aagctggtgg gcgatctgct ggtgctggga
      781 ggagccacac tgtacggcat ctctaacgtg tgggaggagt atatcatcag gaccctgagc
      841 cgcgtggagt ttctgggcat gatcggcctg ttcggcgcct tcttttccgg catccagctg
      901 gccatcatgg agcacaagga gctgctgaag gtgccctggg actggcagat cggcctgctg
      961 tacgtgggct tctccgcctg catgtttggc ctgtattctt tcatgcctgt ggtcatcaag
     1021 aagaccagcg ccaccagcgt gaacctgagc ctgctgacag ccgatctgta ctccctgttt
     1081 tgtggcctgt tcctgtttca ctacaagttc agcggcctgt atctgctgtc cttctttacc
     1141 atcctgatcg gcctggtgct gtactctagc acaagcacct atatcgccca ggacccacgg
     1201 gtgtacaagc agttcagaaa tccctccggc cctgtggtgg atctgccaac aaccgcccag
     1261 gtggagccct ccgtgacata tacctctctg ggacaggaga ccgaggagga gccacacgtg
     1321 cgggtggcct aagacccagc tttcttgtac aaagttggca ttataagaaa gcattgctta
     1381 tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata aaatcattat ttgccatcca
     1441 gctgatatcc cctatagtga gtcgtattac atggtcatag ctgtttcctg gcagctctgg
     1501 cccgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgaaca
     1561 ataaaactgt ctgcttacat aaacagtaat acaaggggtg ttatgagcca tattcaacgg
     1621 gaaacgtcga ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg
     1681 gctcgcgata atgtcgggca atcaggtgcg acaatctatc gcttgtatgg gaagcccgat
     1741 gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag
     1801 atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc
     1861 cgtactcctg atgatgcatg gttactcacc actgcgatcc ccggaaaaac agcattccag
     1921 gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg
     1981 cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt
     2041 ctcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac
     2101 gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataaact tttgccattc
     2161 tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag
     2221 gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat
     2281 cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt
     2341 caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat
     2401 gagtttttct aatcagaatt ggttaattgg ttgtaacact ggcagagcat tacgctgact
     2461 tgacgggacg gcgcaagctc atgaccaaaa tcccttaacg tgagttacgc gtcgttccac
     2521 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     2581 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     2641 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     2701 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     2761 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     2821 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     2881 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     2941 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     3001 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     3061 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     3121 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     3181 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     3241 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     3301 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     3361 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     3421 gagcgcaacg caattaatac gcgtaccgct agccaggaag agtttgtaga aacgcaaaaa
     3481 ggccatccgt caggatggcc ttctgcttag tttgatgcct ggcagtttat ggcgggcgtc
     3541 ctgcccgcca ccctccgggc cgttgcttca caacgttcaa atccgctccc ggcggatttg
     3601 tcctactcag gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtcttccg
     3661 actgagcctt tcgttttatt tgatgcctgg cagttcccta ctctcgcgtt aacgctagca
     3721 tggatgtttt cccagtcacg acgttgtaaa acgacggcca gtcttaagct cgggccc
//
