LOCUS       sequence-345412-        5858 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5858)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_1049:Dendra
FEATURES             Location/Qualifiers
     source          1..5858
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     748..767
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        748..766
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2504..2527
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2784..3584
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3709..4297
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4198..4217
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccgtacgaag atgaacaagg gccccgaggc attcctggtg accacgacgc agggcaaccg
       61 gctgtcgaag tccgcgttca ccaagtcgct gaagcgtggc tacgccaaga tcggtcggcc
      121 ggaactccgc atccacgacc tccgcgctgt cggcgctacg ttcgccgctc aggcaggtgc
      181 gacgaccaag gagctgatgg cccgtctcgg tcacacgact cctaggatgg cgatgaagta
      241 ccagatggcg tctgaggccc gcgacgaggc tatcgctgag gcgatgtcca agctggccaa
      301 gacctcctga aacgcaaaaa gcccccctcc caaggacact gagtcctaaa gaggggggtt
      361 tcttgtcagt acgcgaagaa ccacgcctgg ccgcgagcgc cagcaccgcc gctctgtgcg
      421 gagacctggg caccagcccc gccgccgcca ggagcattgc cgttcccgcc agaaatctag
      481 acggtgacca caacgcgccc gctttgatcg gggacgtcga aatattggat cgtcggcacc
      541 gtcacggccg tgggaggcgg cacgatccgc gacgtgatga tcggccgcat ccccacggtg
      601 ctgcgcagtg agctctacgc catcccggcg ttgatctgtg cgttcgcacg cacaggcccg
      661 gtgtgagaag ggtctctgac gagcgggaga actccctatc agtgatagag tttgtcctcc
      721 ctatcagtga tagataggct taaggagtaa tacgactcac tatagggaga aggaggccat
      781 tatgacggaa atcagggacg ccgccgaccc ggctccgaac ccgcacgcca cggccgaaga
      841 ggtcgaagcc gcgatgcacg acagcaagct ggctcaggtc ctctaccacg actgggaggc
      901 cgagacctac gacgagaagt ggtcgatctc gtacgaccag cgctgcatcg actacgcgcg
      961 cggccggttc gacgccatcg tgcccgagga cgagcagcgc aaactgccgt acgaccgggc
     1021 cctcgagctg ggctgcggca ccgggttctt cctgctcaac ctcatccagt ccggcgtggc
     1081 gcgtcgcggt tcggtcaccg acctgtcgcc cggcatggtc aaggtggcca cccgcaacgg
     1141 ccagtcgctg ggcctcgaca tcgacggccg cgtcgccgac gccgaaggca tcccgtacga
     1201 ggacgacacg ttcgacctgg tcgtcgggca tgctgtgctg caccacatcc ccgacgtcga
     1261 actgtcgctg cgcgaggtcg tacgcatcct caagccgggc ggacggttcg tgttcgccgg
     1321 cgaacccacc acggtgggca accggtacgc ccgcgagctg tcgaccctga cgtggcacgc
     1381 gaccacgaac ctgaccaagc tgccgttcct gagcggctgg cggcggcccc aggaagaact
     1441 cgacgagtcc tcacgcgcgg ccgcccttga ggccgtcgtc gacctgcaca cgttcgatcc
     1501 ggcggacctg gagcgcatgg ccaccaacgc cggtgcggtc gaggtgcgca ccgccagcga
     1561 ggaattcacc gccgccatgc tgggctggcc ggtccgcacg ttcgaggccg cggtgccgcc
     1621 gggccgtctg ggctggggct gggccaagtt cgcgttcaac agctggacca cgctgagctg
     1681 ggtggacgcc aacgtctggc gcaaggtcgt gccgaagggc tggttctaca acgtcatggt
     1741 cacgggggtc aaaccctcca agcttggtgc ggccgcgggc aacacgcctg gcatcaatct
     1801 gatcaaggag gacatgcgtg tcaaggtgca tatggagggc aacgtgaacg gtcacgcctt
     1861 cgtgatcgag ggggagggta agggtaagcc ttacgaaggg acccagaccg ccaacctcac
     1921 cgtcaaggag ggcgcccctc tcccattctc gtacgatatc ctcactaccg cggtgcatta
     1981 cgggaaccgc gtgttcacca agtaccccga ggatatccct gactacttca agcagagctt
     2041 cccggaggga tactcctggg agcggacgat gaccttcgaa gacaagggta tctgtacgat
     2101 ccgcagcgac atctcgctgg aaggggactg tttcttccag aacgtccgtt tcaaggggac
     2161 caactttccg ccgaacggcc cggtcatgca gaagaagacc ctcaagtggg agccgagcac
     2221 ggagaagctg cacgttcggg acggcctcct ggtcggcaac atcaacatgg cgctgctgct
     2281 ggaaggcggc ggccactacc tatgcgactt caagaccacg tacaaagcga agaaggtcgt
     2341 ccagctgccc gacgcgcact tcgtggacca ccgcatcgaa atcctgggga acgattcgga
     2401 ctacaacaag gtcaagttgt acgagcacgc tgtggcacgc tatagcccgc tcccgtcgca
     2461 ggtctggttc gaaggcggcg gcggggagaa cctgtatttc caggactaca aggacgacga
     2521 cgacaagtga tttaaataac cccttggggc ctctaaacgg gtcttgaggg gttttttgtt
     2581 aactagcgta cgatcgaatt cctgcagctc acggtaactg atgccgtatt tgcagtacca
     2641 gcgtacggcc cacagaatga tgtcacgctg aaaatgccgg cctttgaatg ggttcatgtg
     2701 cagctccatc agcaaaaggg gatgataagt ttatcaccac cgactatttg caacagtgcc
     2761 gttgatcgtg ctatgatcga ctgatgtcat cagcggtgga gtgcaatgtc gtgcaatacg
     2821 aatggcgaaa agccgagctc atcggtcagc ttctcaacct tggggttacc cccggcggtg
     2881 tgctgctggt ccacagctcc ttccgtagcg tccggcccct cgaagatggg ccacttggac
     2941 tgatcgaggc cctgcgtgct acgctgggtc cgggagggac gctcgtcatg ccctcgtggt
     3001 caggtctgga cgacgagccg ttcgatcctg ccacgtcgcc cgttacaccg gaccttggag
     3061 ttgtctctga cacattctgg cgcctgccaa atgtaaagcg cagcgcccat ccatttgcct
     3121 ttgcggcagc ggggccacag gcagagcaga tcatctctga tccattgccc ctgccacctt
     3181 actcgcctgc aagcccggtc gcccgtgtcc atgaactcga tgggcaggta cttctcctcg
     3241 gcgtgggaca cgatgccaac acgacgctgc atcttgccga gttgatggca aaggttccct
     3301 atggggtgcc gagacactgc accattcttc aggatggcaa gttggtacgc gtcgattatc
     3361 tcgagaatga ccactgctgt gagcgctttg ccttggcgga caggtggctc aaggagaaga
     3421 gccttcagaa ggaaggtcca gtcggtcatg cctttgctcg gttgatccgc tcccgcgaca
     3481 ttgtggcgac agccctgggt caactgggcc gagatccgtt gatcttcctg catccgccag
     3541 aggcgggatg cgaagaatgc gatgccgctc gccagtcgat tggctgagct catgagcgga
     3601 gaacgagatg acgttggagg ggcaaggtcg cgctgattgc tggggcaaca cgggggatcc
     3661 actagttcca tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     3721 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     3781 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     3841 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     3901 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     3961 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     4021 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     4081 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgagggga gaaaggcgga
     4141 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     4201 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     4261 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     4321 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     4381 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     4441 gaccgagcgc aacgcgtgcg gccgcggtac ccggggatcc tctagagtcg accaccaagg
     4501 gcaccatctc tgcttgggcc accccgttgg ccgcagccag ctcgctgaga gccgtgaacg
     4561 acagggcgaa cgccagcccg ccgacggcga gggttccgac cgctgcaact cccggtgcaa
     4621 ccttgtcccg gtctattctc ttcactgcac cagctccaat ctggtgtgaa tgcccctcgt
     4681 ctgttcgcgc aggcgggggg ctctattcgt ttgtcagcat cgaaagtagc cagatcaggg
     4741 atgcgttgca accgcgtatg cccaggtcag aagagtcgca caagagttgc agacccctgg
     4801 aaagaaaaat ggccagaggg cgaaaacacc ctctgaccag cggagcgggc gacgggaatc
     4861 gaacccgcgt agctagtttg gaagaatggg tgtctgccga ccacatatgg gccggtcaag
     4921 ataggttttt accccctctc ggctgcatcc tctaagtgga aagaaattgc aggtcgtaga
     4981 agcgcgttga agcctgagag ttgcacagga gttgcaaccc ggtagccttg ttcacgacga
     5041 gaggagacct agttggcacg tcgcggatgg ggatcgctga agactcagcg cagcgggagg
     5101 atccaagcct catacgtcaa cccgcaggac ggtgtgaggt actacgcgct gcagacctac
     5161 gacaacaaga tggacgccga agcctggctc gcgggcgaga agcggctcat cgagatggag
     5221 acctggaccc ctccacagga ccgggcgaag aaggcagccg ccagcgccat cacgctggag
     5281 gagtacaccc ggaagtggct cgtggagcgc gacctcgcag acggcaccag ggatctgtac
     5341 agcgggcacg cggagcgccg catctacccg gtgctaggtg aagtggcggt cacagagatg
     5401 acgccagctc tggtgcgtgc gtggtgggcc gggatgggta ggaagcaccc gactgcccgc
     5461 cggcatgcct acaacgtcct ccgggcggtg atgaacacag cggtcgagga caagctgatc
     5521 gcagagaacc cgtgccggat cgagcagaag gcagccgatg agcgcgacgt agaggcgctg
     5581 acgcctgagg agctggacat cgtcgccgct gagatcttcg agcactaccg gatcgcggca
     5641 tacatcctgg cgtggacgag cctccggttc ggagagctga tcgagcttcg ccgcaaggac
     5701 atcgtggacg acggcatgac gatgaagctc cgggtgcgcc gtggcgcttc ccgcgtgggg
     5761 aacaagatcg tcgttggcaa cgccaagacc gtccggtcga agcgtcctgt gacggttccg
     5821 cctcacgtcg cggagatgat ccgagcgcac atgaagga
//
