LOCUS       sequence-345413-        5756 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5756)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_1108:Dendra
FEATURES             Location/Qualifiers
     source          1..5756
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     727..746
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        727..745
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2381..2404
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2661..3461
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3586..4174
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4075..4094
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccccgaggca ttcctggtga ccacgacgca gggcaaccgg ctgtcgaagt ccgcgttcac
       61 caagtcgctg aagcgtggct acgccaagat cggtcggccg gaactccgca tccacgacct
      121 ccgcgctgtc ggcgctacgt tcgccgctca ggcaggtgcg acgaccaagg agctgatggc
      181 ccgtctcggt cacacgactc ctaggatggc gatgaagtac cagatggcgt ctgaggcccg
      241 cgacgaggct atcgctgagg cgatgtccaa gctggccaag acctcctgaa acgcaaaaag
      301 cccccctccc aaggacactg agtcctaaag aggggggttt cttgtcagta cgcgaagaac
      361 cacgcctggc cgcgagcgcc agcaccgccg ctctgtgcgg agacctgggc accagccccg
      421 ccgccgccag gagcattgcc gttcccgcca gaaatctaga cggtgaccac aacgcgcccg
      481 ctttgatcgg ggacgtcgaa atattggatc gtcggcaccg tcacggccgt gggaggcggc
      541 acgatccgcg acgtgatgat cggccgcatc cccacggtgc tgcgcagtga gctctacgcc
      601 atcccggcgt tgatctgtgc gttcgcacgc acaggcccgg tgtgagaagg gtctctgacg
      661 agcgggagaa ctccctatca gtgatagagt ttgtcctccc tatcagtgat agataggctt
      721 aaggagtaat acgactcact atagggagaa ggaggccatt atgcggcacg tcgtaccgcg
      781 tgagctgagg caccgagcgt catcaagtag cgtcagccac gtgaacttgg cttacgacga
      841 acgaggtaaa ggcgaagcgg ttctgttcat cgccggccgc ggtggggcag gccgcacctg
      901 gcatctcaac caggtaccgg agttccagcg cgccgggtac cgcgtcatca cgttcgacaa
      961 ccgcggggtg ggcgccaccg agaacgccga gggtttcggc accgagcaga tggtcgccga
     1021 caccgccgcg ctcatcgaga agctcgacgc cgcgccggca cgcctggtgg gggtgtcgat
     1081 gggatcgttc atcgcgcagg agctcatggt ggcccggccc gaactggtgc actcggcggc
     1141 gttgatggcc acgcgcggcc gccacgacca cgcccgcgag ttcttccgca aggccgagct
     1201 ggacctgtcg cagtccggtg tcgatttgcc gccgaccttc gacgcgaaag tccgcatgct
     1261 ggagagcttt tcgcccaaaa ccctcaatga cgacgcgttc gtgcgggact ggagcgagat
     1321 gttcaccatg tggccgacca agcagacccc cggttcgcgc acccagctcg acgtgggacc
     1381 ggtgaccaac cggctgcccg cgtaccggtc catcagccac ccggtgctgg tcatcgggtt
     1441 cgccgacgac gtcgtgctgc ccgcccacct gggccgcgag gtcgccgacg cgatccccaa
     1501 cggccgctac ctgcagatcc ccgacaccgg gcacctgggc ttcatcgaga agccgcagga
     1561 ggtgaacgcc gcgatcttga acttctttgc cgagcacggc gggaaaaagt tcaccaacct
     1621 gctggcgaat aagctgaagc ttggtgcggc cgcgggcaac acgcctggca tcaatctgat
     1681 caaggaggac atgcgtgtca aggtgcatat ggagggcaac gtgaacggtc acgccttcgt
     1741 gatcgagggg gagggtaagg gtaagcctta cgaagggacc cagaccgcca acctcaccgt
     1801 caaggagggc gcccctctcc cattctcgta cgatatcctc actaccgcgg tgcattacgg
     1861 gaaccgcgtg ttcaccaagt accccgagga tatccctgac tacttcaagc agagcttccc
     1921 ggagggatac tcctgggagc ggacgatgac cttcgaagac aagggtatct gtacgatccg
     1981 cagcgacatc tcgctggaag gggactgttt cttccagaac gtccgtttca aggggaccaa
     2041 ctttccgccg aacggcccgg tcatgcagaa gaagaccctc aagtgggagc cgagcacgga
     2101 gaagctgcac gttcgggacg gcctcctggt cggcaacatc aacatggcgc tgctgctgga
     2161 aggcggcggc cactacctat gcgacttcaa gaccacgtac aaagcgaaga aggtcgtcca
     2221 gctgcccgac gcgcacttcg tggaccaccg catcgaaatc ctggggaacg attcggacta
     2281 caacaaggtc aagttgtacg agcacgctgt ggcacgctat agcccgctcc cgtcgcaggt
     2341 ctggttcgaa ggcggcggcg gggagaacct gtatttccag gactacaagg acgacgacga
     2401 caagtgattt aaataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgttaac
     2461 tagcgtacga tcgaattcct gcagctcacg gtaactgatg ccgtatttgc agtaccagcg
     2521 tacggcccac agaatgatgt cacgctgaaa atgccggcct ttgaatgggt tcatgtgcag
     2581 ctccatcagc aaaaggggat gataagttta tcaccaccga ctatttgcaa cagtgccgtt
     2641 gatcgtgcta tgatcgactg atgtcatcag cggtggagtg caatgtcgtg caatacgaat
     2701 ggcgaaaagc cgagctcatc ggtcagcttc tcaaccttgg ggttaccccc ggcggtgtgc
     2761 tgctggtcca cagctccttc cgtagcgtcc ggcccctcga agatgggcca cttggactga
     2821 tcgaggccct gcgtgctacg ctgggtccgg gagggacgct cgtcatgccc tcgtggtcag
     2881 gtctggacga cgagccgttc gatcctgcca cgtcgcccgt tacaccggac cttggagttg
     2941 tctctgacac attctggcgc ctgccaaatg taaagcgcag cgcccatcca tttgcctttg
     3001 cggcagcggg gccacaggca gagcagatca tctctgatcc attgcccctg ccaccttact
     3061 cgcctgcaag cccggtcgcc cgtgtccatg aactcgatgg gcaggtactt ctcctcggcg
     3121 tgggacacga tgccaacacg acgctgcatc ttgccgagtt gatggcaaag gttccctatg
     3181 gggtgccgag acactgcacc attcttcagg atggcaagtt ggtacgcgtc gattatctcg
     3241 agaatgacca ctgctgtgag cgctttgcct tggcggacag gtggctcaag gagaagagcc
     3301 ttcagaagga aggtccagtc ggtcatgcct ttgctcggtt gatccgctcc cgcgacattg
     3361 tggcgacagc cctgggtcaa ctgggccgag atccgttgat cttcctgcat ccgccagagg
     3421 cgggatgcga agaatgcgat gccgctcgcc agtcgattgg ctgagctcat gagcggagaa
     3481 cgagatgacg ttggaggggc aaggtcgcgc tgattgctgg ggcaacacgg gggatccact
     3541 agttccatga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     3601 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     3661 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     3721 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     3781 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     3841 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     3901 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     3961 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaggggagaa aggcggacag
     4021 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     4081 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     4141 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     4201 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     4261 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     4321 cgagcgcaac gcgtgcggcc gcggtacccg gggatcctct agagtcgacc accaagggca
     4381 ccatctctgc ttgggccacc ccgttggccg cagccagctc gctgagagcc gtgaacgaca
     4441 gggcgaacgc cagcccgccg acggcgaggg ttccgaccgc tgcaactccc ggtgcaacct
     4501 tgtcccggtc tattctcttc actgcaccag ctccaatctg gtgtgaatgc ccctcgtctg
     4561 ttcgcgcagg cggggggctc tattcgtttg tcagcatcga aagtagccag atcagggatg
     4621 cgttgcaacc gcgtatgccc aggtcagaag agtcgcacaa gagttgcaga cccctggaaa
     4681 gaaaaatggc cagagggcga aaacaccctc tgaccagcgg agcgggcgac gggaatcgaa
     4741 cccgcgtagc tagtttggaa gaatgggtgt ctgccgacca catatgggcc ggtcaagata
     4801 ggtttttacc ccctctcggc tgcatcctct aagtggaaag aaattgcagg tcgtagaagc
     4861 gcgttgaagc ctgagagttg cacaggagtt gcaacccggt agccttgttc acgacgagag
     4921 gagacctagt tggcacgtcg cggatgggga tcgctgaaga ctcagcgcag cgggaggatc
     4981 caagcctcat acgtcaaccc gcaggacggt gtgaggtact acgcgctgca gacctacgac
     5041 aacaagatgg acgccgaagc ctggctcgcg ggcgagaagc ggctcatcga gatggagacc
     5101 tggacccctc cacaggaccg ggcgaagaag gcagccgcca gcgccatcac gctggaggag
     5161 tacacccgga agtggctcgt ggagcgcgac ctcgcagacg gcaccaggga tctgtacagc
     5221 gggcacgcgg agcgccgcat ctacccggtg ctaggtgaag tggcggtcac agagatgacg
     5281 ccagctctgg tgcgtgcgtg gtgggccggg atgggtagga agcacccgac tgcccgccgg
     5341 catgcctaca acgtcctccg ggcggtgatg aacacagcgg tcgaggacaa gctgatcgca
     5401 gagaacccgt gccggatcga gcagaaggca gccgatgagc gcgacgtaga ggcgctgacg
     5461 cctgaggagc tggacatcgt cgccgctgag atcttcgagc actaccggat cgcggcatac
     5521 atcctggcgt ggacgagcct ccggttcgga gagctgatcg agcttcgccg caaggacatc
     5581 gtggacgacg gcatgacgat gaagctccgg gtgcgccgtg gcgcttcccg cgtggggaac
     5641 aagatcgtcg ttggcaacgc caagaccgtc cggtcgaagc gtcctgtgac ggttccgcct
     5701 cacgtcgcgg agatgatccg agcgcacatg aaggaccgta cgaagatgaa caaggg
//
