LOCUS       sequence-345423-        5531 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5531)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5531)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0971:Dendra
FEATURES             Location/Qualifiers
     source          1..5531
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     717..736
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        717..735
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2146..2169
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2426..3226
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3351..3939
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3840..3859
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttcctggtga ccacgacgca gggcaaccgg ctgtcgaagt ccgcgttcac caagtcgctg
       61 aagcgtggct acgccaagat cggtcggccg gaactccgca tccacgacct ccgcgctgtc
      121 ggcgctacgt tcgccgctca ggcaggtgcg acgaccaagg agctgatggc ccgtctcggt
      181 cacacgactc ctaggatggc gatgaagtac cagatggcgt ctgaggcccg cgacgaggct
      241 atcgctgagg cgatgtccaa gctggccaag acctcctgaa acgcaaaaag cccccctccc
      301 aaggacactg agtcctaaag aggggggttt cttgtcagta cgcgaagaac cacgcctggc
      361 cgcgagcgcc agcaccgccg ctctgtgcgg agacctgggc accagccccg ccgccgccag
      421 gagcattgcc gttcccgcca gaaatctaga cggtgaccac aacgcgcccg ctttgatcgg
      481 ggacgtcgaa atattggatc gtcggcaccg tcacggccgt gggaggcggc acgatccgcg
      541 acgtgatgat cggccgcatc cccacggtgc tgcgcagtga gctctacgcc atcccggcgt
      601 tgatctgtgc gttcgcacgc acaggcccgg tgtgagaagg gtctctgacg agcgggagaa
      661 ctccctatca gtgatagagt ttgtcctccc tatcagtgat agataggctt aaggagtaat
      721 acgactcact atagggagaa ggaggccatt atgcagtcgt gggtcgcccg tgtggtgggt
      781 gtgacgctgg cggcgctgtt cgcagtgctg acgggtgcgc tcgccggttg ctcgacgggt
      841 gacgatgcgg tggcccaggg tggcacgttc gagttcgtcg ccccgggcgg caagactgac
      901 atcttctatg atccgcccca aagccgcggc cgcccaggca cattggccgg acccttgctc
      961 accgacccgt cgaagaccat ctcgctcgac gatttcgcgg gcgaggtcgt ggtggtcaac
     1021 gtgtggggtc agtggtgcgg gccgtgccgc gcggagatca cgcagctgca agaggtccac
     1081 aacgccacca aggacaaggg cgtggcgttc ctcggcatcg acgtgcgcga caacaaccgc
     1141 gacgccgcag tcgatttcgt gaaggaccgc aacgtcacgt tcccgtcgat ctacgacccg
     1201 cccatgcgca cgatgatcgc gttcggcggc aagtacccca ccacggtcat cccgtccacg
     1261 gtggtgctgg accgtcagca ccgcgtggcg gcggtgttcc tgcgtgaact gctcgccgag
     1321 gatctcgagc cgctcatcga acgtctgctc gccgaaccgg ctgagcccgg cgagcccgcg
     1381 gtgtcgaaag agcagcggcc gaagcttggt gcggccgcgg gcaacacgcc tggcatcaat
     1441 ctgatcaagg aggacatgcg tgtcaaggtg catatggagg gcaacgtgaa cggtcacgcc
     1501 ttcgtgatcg agggggaggg taagggtaag ccttacgaag ggacccagac cgccaacctc
     1561 accgtcaagg agggcgcccc tctcccattc tcgtacgata tcctcactac cgcggtgcat
     1621 tacgggaacc gcgtgttcac caagtacccc gaggatatcc ctgactactt caagcagagc
     1681 ttcccggagg gatactcctg ggagcggacg atgaccttcg aagacaaggg tatctgtacg
     1741 atccgcagcg acatctcgct ggaaggggac tgtttcttcc agaacgtccg tttcaagggg
     1801 accaactttc cgccgaacgg cccggtcatg cagaagaaga ccctcaagtg ggagccgagc
     1861 acggagaagc tgcacgttcg ggacggcctc ctggtcggca acatcaacat ggcgctgctg
     1921 ctggaaggcg gcggccacta cctatgcgac ttcaagacca cgtacaaagc gaagaaggtc
     1981 gtccagctgc ccgacgcgca cttcgtggac caccgcatcg aaatcctggg gaacgattcg
     2041 gactacaaca aggtcaagtt gtacgagcac gctgtggcac gctatagccc gctcccgtcg
     2101 caggtctggt tcgaaggcgg cggcggggag aacctgtatt tccaggacta caaggacgac
     2161 gacgacaagt gatttaaata accccttggg gcctctaaac gggtcttgag gggttttttg
     2221 ttaactagcg tacgatcgaa ttcctgcagc tcacggtaac tgatgccgta tttgcagtac
     2281 cagcgtacgg cccacagaat gatgtcacgc tgaaaatgcc ggcctttgaa tgggttcatg
     2341 tgcagctcca tcagcaaaag gggatgataa gtttatcacc accgactatt tgcaacagtg
     2401 ccgttgatcg tgctatgatc gactgatgtc atcagcggtg gagtgcaatg tcgtgcaata
     2461 cgaatggcga aaagccgagc tcatcggtca gcttctcaac cttggggtta cccccggcgg
     2521 tgtgctgctg gtccacagct ccttccgtag cgtccggccc ctcgaagatg ggccacttgg
     2581 actgatcgag gccctgcgtg ctacgctggg tccgggaggg acgctcgtca tgccctcgtg
     2641 gtcaggtctg gacgacgagc cgttcgatcc tgccacgtcg cccgttacac cggaccttgg
     2701 agttgtctct gacacattct ggcgcctgcc aaatgtaaag cgcagcgccc atccatttgc
     2761 ctttgcggca gcggggccac aggcagagca gatcatctct gatccattgc ccctgccacc
     2821 ttactcgcct gcaagcccgg tcgcccgtgt ccatgaactc gatgggcagg tacttctcct
     2881 cggcgtggga cacgatgcca acacgacgct gcatcttgcc gagttgatgg caaaggttcc
     2941 ctatggggtg ccgagacact gcaccattct tcaggatggc aagttggtac gcgtcgatta
     3001 tctcgagaat gaccactgct gtgagcgctt tgccttggcg gacaggtggc tcaaggagaa
     3061 gagccttcag aaggaaggtc cagtcggtca tgcctttgct cggttgatcc gctcccgcga
     3121 cattgtggcg acagccctgg gtcaactggg ccgagatccg ttgatcttcc tgcatccgcc
     3181 agaggcggga tgcgaagaat gcgatgccgc tcgccagtcg attggctgag ctcatgagcg
     3241 gagaacgaga tgacgttgga ggggcaaggt cgcgctgatt gctggggcaa cacgggggat
     3301 ccactagttc catgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     3361 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     3421 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     3481 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
     3541 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     3601 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     3661 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     3721 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaggg gagaaaggcg
     3781 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     3841 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     3901 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     3961 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     4021 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     4081 acgaccgagc gcaacgcgtg cggccgcggt acccggggat cctctagagt cgaccaccaa
     4141 gggcaccatc tctgcttggg ccaccccgtt ggccgcagcc agctcgctga gagccgtgaa
     4201 cgacagggcg aacgccagcc cgccgacggc gagggttccg accgctgcaa ctcccggtgc
     4261 aaccttgtcc cggtctattc tcttcactgc accagctcca atctggtgtg aatgcccctc
     4321 gtctgttcgc gcaggcgggg ggctctattc gtttgtcagc atcgaaagta gccagatcag
     4381 ggatgcgttg caaccgcgta tgcccaggtc agaagagtcg cacaagagtt gcagacccct
     4441 ggaaagaaaa atggccagag ggcgaaaaca ccctctgacc agcggagcgg gcgacgggaa
     4501 tcgaacccgc gtagctagtt tggaagaatg ggtgtctgcc gaccacatat gggccggtca
     4561 agataggttt ttaccccctc tcggctgcat cctctaagtg gaaagaaatt gcaggtcgta
     4621 gaagcgcgtt gaagcctgag agttgcacag gagttgcaac ccggtagcct tgttcacgac
     4681 gagaggagac ctagttggca cgtcgcggat ggggatcgct gaagactcag cgcagcggga
     4741 ggatccaagc ctcatacgtc aacccgcagg acggtgtgag gtactacgcg ctgcagacct
     4801 acgacaacaa gatggacgcc gaagcctggc tcgcgggcga gaagcggctc atcgagatgg
     4861 agacctggac ccctccacag gaccgggcga agaaggcagc cgccagcgcc atcacgctgg
     4921 aggagtacac ccggaagtgg ctcgtggagc gcgacctcgc agacggcacc agggatctgt
     4981 acagcgggca cgcggagcgc cgcatctacc cggtgctagg tgaagtggcg gtcacagaga
     5041 tgacgccagc tctggtgcgt gcgtggtggg ccgggatggg taggaagcac ccgactgccc
     5101 gccggcatgc ctacaacgtc ctccgggcgg tgatgaacac agcggtcgag gacaagctga
     5161 tcgcagagaa cccgtgccgg atcgagcaga aggcagccga tgagcgcgac gtagaggcgc
     5221 tgacgcctga ggagctggac atcgtcgccg ctgagatctt cgagcactac cggatcgcgg
     5281 catacatcct ggcgtggacg agcctccggt tcggagagct gatcgagctt cgccgcaagg
     5341 acatcgtgga cgacggcatg acgatgaagc tccgggtgcg ccgtggcgct tcccgcgtgg
     5401 ggaacaagat cgtcgttggc aacgccaaga ccgtccggtc gaagcgtcct gtgacggttc
     5461 cgcctcacgt cgcggagatg atccgagcgc acatgaagga ccgtacgaag atgaacaagg
     5521 gccccgaggc a
//
