LOCUS       sequence-345460-        6095 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6095)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 6095)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0769:Dendra
FEATURES             Location/Qualifiers
     source          1..6095
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     342..361
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        342..360
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2335..2358
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2615..3415
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3540..4128
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4029..4048
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgccgctctg tgcggagacc tgggcaccag ccccgccgcc gccaggagca ttgccgttcc
       61 cgccagaaat ctagacggtg accacaacgc gcccgctttg atcggggacg tcgaaatatt
      121 ggatcgtcgg caccgtcacg gccgtgggag gcggcacgat ccgcgacgtg atgatcggcc
      181 gcatccccac ggtgctgcgc agtgagctct acgccatccc ggcgttgatc tgtgcgttcg
      241 cacgcacagg cccggtgtga gaagggtctc tgacgagcgg gagaactccc tatcagtgat
      301 agagtttgtc ctccctatca gtgatagata ggcttaagga gtaatacgac tcactatagg
      361 gagaaggagg ccattatgac cgacgacatc ccatccgttc gcatcccggc gcccctgccc
      421 gacggcgtga gccaggcgac catcggcgtg cgcggcggcc tgctgcgttc gggtttcgaa
      481 gagaccgccg aggcgatgta tctgacgtcg ggttacgtct acgagaccgc cgcggccgcc
      541 gagaaggcgt tcaccggcga catcgaccgc tacgtgtact cgcgttacgg caacccgacg
      601 atatcgatgt tcgaggagcg gttgcggctc atcgagggcg cgcccgcgtg tttcgcgacc
      661 gccaccggca tggccgcggt gttcaccgcg ctcggggcgc tgctgggggc gggggaccgg
      721 ttggtcgccg cgcgcagcct gttcggatcg tgtttcgtgg tgtgcaacga gatcctgccg
      781 cgctgggggg tcgagaccgt gttcgtcgac ggcgacgatc tctcgcagtg ggaggaagcc
      841 ctttcggtgc ccacgacggc ggtgttcttc gagacgccgt cgaaccccat gcagtcgctg
      901 gtcgacatcg ccgcggtatc ggagatggcc cacgccgcag gtgcgaaagt ggtgctggac
      961 aatgtgttcg ccacaccgct gctgcagcag ggcatcccga tgggcgccga tgtggtggtg
     1021 tactcgggca ccaagcacat cgacggccag ggccgtgtgc tcggcggggc catcctcggt
     1081 gatcaggagt acatcgacgg cccggtgcag aagctcatgc gccacaccgg ccccgcgatc
     1141 agcgcgttca acgcctgggt gctgctgaaa ggtcttgaga cgcttgcggt ccgggtggac
     1201 tattccaacc ggtcggcaca gcgggtcgcc gagttcctgg aaggccaccc ggccgtgcgc
     1261 tgggtgaaat acccgttcct ccagtcgcat ccgcagtacg agctggccaa gcggcagatg
     1321 cgcggcggtg gcaccgtggt gaccttcgaa ctcgacggcg acgacggcaa ggcccgcgcg
     1381 ttcgaggtgc tcgacaagct gcgcgtcatc gacatctcca acaacctggg tgacgccaaa
     1441 tcgctgatca cgcaccccgc caccacgacc caccgggcca tgggcccgga gggccgcgcc
     1501 gcgatcggcc tgggagacgg tgtcgtgcgc atctcgatcg gcctggaggg caccgaggac
     1561 ctgatcgccg acctcgacca ggcgctgagc aagcttggtg cggccgcggg caacacgcct
     1621 ggcatcaatc tgatcaagga ggacatgcgt gtcaaggtgc atatggaggg caacgtgaac
     1681 ggtcacgcct tcgtgatcga gggggagggt aagggtaagc cttacgaagg gacccagacc
     1741 gccaacctca ccgtcaagga gggcgcccct ctcccattct cgtacgatat cctcactacc
     1801 gcggtgcatt acgggaaccg cgtgttcacc aagtaccccg aggatatccc tgactacttc
     1861 aagcagagct tcccggaggg atactcctgg gagcggacga tgaccttcga agacaagggt
     1921 atctgtacga tccgcagcga catctcgctg gaaggggact gtttcttcca gaacgtccgt
     1981 ttcaagggga ccaactttcc gccgaacggc ccggtcatgc agaagaagac cctcaagtgg
     2041 gagccgagca cggagaagct gcacgttcgg gacggcctcc tggtcggcaa catcaacatg
     2101 gcgctgctgc tggaaggcgg cggccactac ctatgcgact tcaagaccac gtacaaagcg
     2161 aagaaggtcg tccagctgcc cgacgcgcac ttcgtggacc accgcatcga aatcctgggg
     2221 aacgattcgg actacaacaa ggtcaagttg tacgagcacg ctgtggcacg ctatagcccg
     2281 ctcccgtcgc aggtctggtt cgaaggcggc ggcggggaga acctgtattt ccaggactac
     2341 aaggacgacg acgacaagtg atttaaataa ccccttgggg cctctaaacg ggtcttgagg
     2401 ggttttttgt taactagcgt acgatcgaat tcctgcagct cacggtaact gatgccgtat
     2461 ttgcagtacc agcgtacggc ccacagaatg atgtcacgct gaaaatgccg gcctttgaat
     2521 gggttcatgt gcagctccat cagcaaaagg ggatgataag tttatcacca ccgactattt
     2581 gcaacagtgc cgttgatcgt gctatgatcg actgatgtca tcagcggtgg agtgcaatgt
     2641 cgtgcaatac gaatggcgaa aagccgagct catcggtcag cttctcaacc ttggggttac
     2701 ccccggcggt gtgctgctgg tccacagctc cttccgtagc gtccggcccc tcgaagatgg
     2761 gccacttgga ctgatcgagg ccctgcgtgc tacgctgggt ccgggaggga cgctcgtcat
     2821 gccctcgtgg tcaggtctgg acgacgagcc gttcgatcct gccacgtcgc ccgttacacc
     2881 ggaccttgga gttgtctctg acacattctg gcgcctgcca aatgtaaagc gcagcgccca
     2941 tccatttgcc tttgcggcag cggggccaca ggcagagcag atcatctctg atccattgcc
     3001 cctgccacct tactcgcctg caagcccggt cgcccgtgtc catgaactcg atgggcaggt
     3061 acttctcctc ggcgtgggac acgatgccaa cacgacgctg catcttgccg agttgatggc
     3121 aaaggttccc tatggggtgc cgagacactg caccattctt caggatggca agttggtacg
     3181 cgtcgattat ctcgagaatg accactgctg tgagcgcttt gccttggcgg acaggtggct
     3241 caaggagaag agccttcaga aggaaggtcc agtcggtcat gcctttgctc ggttgatccg
     3301 ctcccgcgac attgtggcga cagccctggg tcaactgggc cgagatccgt tgatcttcct
     3361 gcatccgcca gaggcgggat gcgaagaatg cgatgccgct cgccagtcga ttggctgagc
     3421 tcatgagcgg agaacgagat gacgttggag gggcaaggtc gcgctgattg ctggggcaac
     3481 acgggggatc cactagttcc atgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3541 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3601 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3661 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     3721 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3781 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3841 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3901 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgagggg
     3961 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     4021 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     4081 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     4141 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     4201 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     4261 cgcagccgaa cgaccgagcg caacgcgtgc ggccgcggta cccggggatc ctctagagtc
     4321 gaccaccaag ggcaccatct ctgcttgggc caccccgttg gccgcagcca gctcgctgag
     4381 agccgtgaac gacagggcga acgccagccc gccgacggcg agggttccga ccgctgcaac
     4441 tcccggtgca accttgtccc ggtctattct cttcactgca ccagctccaa tctggtgtga
     4501 atgcccctcg tctgttcgcg caggcggggg gctctattcg tttgtcagca tcgaaagtag
     4561 ccagatcagg gatgcgttgc aaccgcgtat gcccaggtca gaagagtcgc acaagagttg
     4621 cagacccctg gaaagaaaaa tggccagagg gcgaaaacac cctctgacca gcggagcggg
     4681 cgacgggaat cgaacccgcg tagctagttt ggaagaatgg gtgtctgccg accacatatg
     4741 ggccggtcaa gataggtttt taccccctct cggctgcatc ctctaagtgg aaagaaattg
     4801 caggtcgtag aagcgcgttg aagcctgaga gttgcacagg agttgcaacc cggtagcctt
     4861 gttcacgacg agaggagacc tagttggcac gtcgcggatg gggatcgctg aagactcagc
     4921 gcagcgggag gatccaagcc tcatacgtca acccgcagga cggtgtgagg tactacgcgc
     4981 tgcagaccta cgacaacaag atggacgccg aagcctggct cgcgggcgag aagcggctca
     5041 tcgagatgga gacctggacc cctccacagg accgggcgaa gaaggcagcc gccagcgcca
     5101 tcacgctgga ggagtacacc cggaagtggc tcgtggagcg cgacctcgca gacggcacca
     5161 gggatctgta cagcgggcac gcggagcgcc gcatctaccc ggtgctaggt gaagtggcgg
     5221 tcacagagat gacgccagct ctggtgcgtg cgtggtgggc cgggatgggt aggaagcacc
     5281 cgactgcccg ccggcatgcc tacaacgtcc tccgggcggt gatgaacaca gcggtcgagg
     5341 acaagctgat cgcagagaac ccgtgccgga tcgagcagaa ggcagccgat gagcgcgacg
     5401 tagaggcgct gacgcctgag gagctggaca tcgtcgccgc tgagatcttc gagcactacc
     5461 ggatcgcggc atacatcctg gcgtggacga gcctccggtt cggagagctg atcgagcttc
     5521 gccgcaagga catcgtggac gacggcatga cgatgaagct ccgggtgcgc cgtggcgctt
     5581 cccgcgtggg gaacaagatc gtcgttggca acgccaagac cgtccggtcg aagcgtcctg
     5641 tgacggttcc gcctcacgtc gcggagatga tccgagcgca catgaaggac cgtacgaaga
     5701 tgaacaaggg ccccgaggca ttcctggtga ccacgacgca gggcaaccgg ctgtcgaagt
     5761 ccgcgttcac caagtcgctg aagcgtggct acgccaagat cggtcggccg gaactccgca
     5821 tccacgacct ccgcgctgtc ggcgctacgt tcgccgctca ggcaggtgcg acgaccaagg
     5881 agctgatggc ccgtctcggt cacacgactc ctaggatggc gatgaagtac cagatggcgt
     5941 ctgaggcccg cgacgaggct atcgctgagg cgatgtccaa gctggccaag acctcctgaa
     6001 acgcaaaaag cccccctccc aaggacactg agtcctaaag aggggggttt cttgtcagta
     6061 cgcgaagaac cacgcctggc cgcgagcgcc agcac
//
