LOCUS       sequence-345461-        5738 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5738)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5738)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_1350:Dendra
FEATURES             Location/Qualifiers
     source          1..5738
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     757..776
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        757..775
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2393..2416
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2673..3473
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3598..4186
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4087..4106
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgaaggac cgtacgaaga tgaacaaggg ccccgaggca ttcctggtga ccacgacgca
       61 gggcaaccgg ctgtcgaagt ccgcgttcac caagtcgctg aagcgtggct acgccaagat
      121 cggtcggccg gaactccgca tccacgacct ccgcgctgtc ggcgctacgt tcgccgctca
      181 ggcaggtgcg acgaccaagg agctgatggc ccgtctcggt cacacgactc ctaggatggc
      241 gatgaagtac cagatggcgt ctgaggcccg cgacgaggct atcgctgagg cgatgtccaa
      301 gctggccaag acctcctgaa acgcaaaaag cccccctccc aaggacactg agtcctaaag
      361 aggggggttt cttgtcagta cgcgaagaac cacgcctggc cgcgagcgcc agcaccgccg
      421 ctctgtgcgg agacctgggc accagccccg ccgccgccag gagcattgcc gttcccgcca
      481 gaaatctaga cggtgaccac aacgcgcccg ctttgatcgg ggacgtcgaa atattggatc
      541 gtcggcaccg tcacggccgt gggaggcggc acgatccgcg acgtgatgat cggccgcatc
      601 cccacggtgc tgcgcagtga gctctacgcc atcccggcgt tgatctgtgc gttcgcacgc
      661 acaggcccgg tgtgagaagg gtctctgacg agcgggagaa ctccctatca gtgatagagt
      721 ttgtcctccc tatcagtgat agataggctt aaggagtaat acgactcact atagggagaa
      781 ggaggccatt atgtctaaat tgacacccaa atacgaagaa ctgcagtcga tctacgacat
      841 ttcgaacgag ttttacgagc tgttcctggg ccccaccatg ggctacacgt gcggttattt
      901 cgagcgcgac gacatgacgg gcgacgaggc ccagatcgcg aagttcgacc tcgcgctcgg
      961 caagctcggc ctggaacccg gcatgacact gctcgacatc ggttgcggct ggggcgcctg
     1021 catggcccgc gcggccgaga agtacgacgt caacgtcatc ggcctgacgc tcagcggtga
     1081 acagcgtgaa tacgccatcg aaaagctgtc caaggtgccc accgaccgca agctcgaggt
     1141 ccgcctgcag ggctgggaag agttcgacga gaaggtcgac cgcatcgtgt cgatcggcgc
     1201 cttcgagcac ttcggattcg agcgctatcc cgcgttcttc gacatggcct acaacgcgct
     1261 gcccgacgac ggcgtcatgc tgctgcacaa catcaccggg ttcgacctgc gcgaaggcca
     1321 gaagctcggc ctgcacatga cattcgagga cgcccggttc gcgcgcttca tcatgaccga
     1381 gatcttcccc ggcggccgtc tgccgtcagt ggtcatggag caggagaagg cgatcgaggc
     1441 cggcttcaag ctcacccagc tgcaggagat cggcccgcac tacgtccgca cgctcaagat
     1501 ctgggccgac gcgctcgagg cgaacaagga cgaggccatc gcgatccagg gccaagaggt
     1561 ctacgaccgc tacgacaagt acctcaacgg ctgccagaag tacttcgcct cgggccacat
     1621 cagcgtccac cagttcacgc tgcagaagaa gcttggtgcg gccgcgggca acacgcctgg
     1681 catcaatctg atcaaggagg acatgcgtgt caaggtgcat atggagggca acgtgaacgg
     1741 tcacgccttc gtgatcgagg gggagggtaa gggtaagcct tacgaaggga cccagaccgc
     1801 caacctcacc gtcaaggagg gcgcccctct cccattctcg tacgatatcc tcactaccgc
     1861 ggtgcattac gggaaccgcg tgttcaccaa gtaccccgag gatatccctg actacttcaa
     1921 gcagagcttc ccggagggat actcctggga gcggacgatg accttcgaag acaagggtat
     1981 ctgtacgatc cgcagcgaca tctcgctgga aggggactgt ttcttccaga acgtccgttt
     2041 caaggggacc aactttccgc cgaacggccc ggtcatgcag aagaagaccc tcaagtggga
     2101 gccgagcacg gagaagctgc acgttcggga cggcctcctg gtcggcaaca tcaacatggc
     2161 gctgctgctg gaaggcggcg gccactacct atgcgacttc aagaccacgt acaaagcgaa
     2221 gaaggtcgtc cagctgcccg acgcgcactt cgtggaccac cgcatcgaaa tcctggggaa
     2281 cgattcggac tacaacaagg tcaagttgta cgagcacgct gtggcacgct atagcccgct
     2341 cccgtcgcag gtctggttcg aaggcggcgg cggggagaac ctgtatttcc aggactacaa
     2401 ggacgacgac gacaagtgat ttaaataacc ccttggggcc tctaaacggg tcttgagggg
     2461 ttttttgtta actagcgtac gatcgaattc ctgcagctca cggtaactga tgccgtattt
     2521 gcagtaccag cgtacggccc acagaatgat gtcacgctga aaatgccggc ctttgaatgg
     2581 gttcatgtgc agctccatca gcaaaagggg atgataagtt tatcaccacc gactatttgc
     2641 aacagtgccg ttgatcgtgc tatgatcgac tgatgtcatc agcggtggag tgcaatgtcg
     2701 tgcaatacga atggcgaaaa gccgagctca tcggtcagct tctcaacctt ggggttaccc
     2761 ccggcggtgt gctgctggtc cacagctcct tccgtagcgt ccggcccctc gaagatgggc
     2821 cacttggact gatcgaggcc ctgcgtgcta cgctgggtcc gggagggacg ctcgtcatgc
     2881 cctcgtggtc aggtctggac gacgagccgt tcgatcctgc cacgtcgccc gttacaccgg
     2941 accttggagt tgtctctgac acattctggc gcctgccaaa tgtaaagcgc agcgcccatc
     3001 catttgcctt tgcggcagcg gggccacagg cagagcagat catctctgat ccattgcccc
     3061 tgccacctta ctcgcctgca agcccggtcg cccgtgtcca tgaactcgat gggcaggtac
     3121 ttctcctcgg cgtgggacac gatgccaaca cgacgctgca tcttgccgag ttgatggcaa
     3181 aggttcccta tggggtgccg agacactgca ccattcttca ggatggcaag ttggtacgcg
     3241 tcgattatct cgagaatgac cactgctgtg agcgctttgc cttggcggac aggtggctca
     3301 aggagaagag ccttcagaag gaaggtccag tcggtcatgc ctttgctcgg ttgatccgct
     3361 cccgcgacat tgtggcgaca gccctgggtc aactgggccg agatccgttg atcttcctgc
     3421 atccgccaga ggcgggatgc gaagaatgcg atgccgctcg ccagtcgatt ggctgagctc
     3481 atgagcggag aacgagatga cgttggaggg gcaaggtcgc gctgattgct ggggcaacac
     3541 gggggatcca ctagttccat gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     3601 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     3661 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     3721 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     3781 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     3841 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     3901 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     3961 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaggggag
     4021 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     4081 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     4141 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     4201 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     4261 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     4321 cagccgaacg accgagcgca acgcgtgcgg ccgcggtacc cggggatcct ctagagtcga
     4381 ccaccaaggg caccatctct gcttgggcca ccccgttggc cgcagccagc tcgctgagag
     4441 ccgtgaacga cagggcgaac gccagcccgc cgacggcgag ggttccgacc gctgcaactc
     4501 ccggtgcaac cttgtcccgg tctattctct tcactgcacc agctccaatc tggtgtgaat
     4561 gcccctcgtc tgttcgcgca ggcggggggc tctattcgtt tgtcagcatc gaaagtagcc
     4621 agatcaggga tgcgttgcaa ccgcgtatgc ccaggtcaga agagtcgcac aagagttgca
     4681 gacccctgga aagaaaaatg gccagagggc gaaaacaccc tctgaccagc ggagcgggcg
     4741 acgggaatcg aacccgcgta gctagtttgg aagaatgggt gtctgccgac cacatatggg
     4801 ccggtcaaga taggttttta ccccctctcg gctgcatcct ctaagtggaa agaaattgca
     4861 ggtcgtagaa gcgcgttgaa gcctgagagt tgcacaggag ttgcaacccg gtagccttgt
     4921 tcacgacgag aggagaccta gttggcacgt cgcggatggg gatcgctgaa gactcagcgc
     4981 agcgggagga tccaagcctc atacgtcaac ccgcaggacg gtgtgaggta ctacgcgctg
     5041 cagacctacg acaacaagat ggacgccgaa gcctggctcg cgggcgagaa gcggctcatc
     5101 gagatggaga cctggacccc tccacaggac cgggcgaaga aggcagccgc cagcgccatc
     5161 acgctggagg agtacacccg gaagtggctc gtggagcgcg acctcgcaga cggcaccagg
     5221 gatctgtaca gcgggcacgc ggagcgccgc atctacccgg tgctaggtga agtggcggtc
     5281 acagagatga cgccagctct ggtgcgtgcg tggtgggccg ggatgggtag gaagcacccg
     5341 actgcccgcc ggcatgccta caacgtcctc cgggcggtga tgaacacagc ggtcgaggac
     5401 aagctgatcg cagagaaccc gtgccggatc gagcagaagg cagccgatga gcgcgacgta
     5461 gaggcgctga cgcctgagga gctggacatc gtcgccgctg agatcttcga gcactaccgg
     5521 atcgcggcat acatcctggc gtggacgagc ctccggttcg gagagctgat cgagcttcgc
     5581 cgcaaggaca tcgtggacga cggcatgacg atgaagctcc gggtgcgccg tggcgcttcc
     5641 cgcgtgggga acaagatcgt cgttggcaac gccaagaccg tccggtcgaa gcgtcctgtg
     5701 acggttccgc ctcacgtcgc ggagatgatc cgagcgca
//
