LOCUS       sequence-345563-        2376 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression Recording Islands (XRIs) for recording cellular 
            physiological histories along intracellular protein self-assembly. 
            Contains XRI subunit with FLAG tag, under UBC promoter with a FLEX 
            switch..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Linghu C, An B, Shpokayte M, Celiker OT, Shmoel N, Zhang R, Zhang C,
            Park D, Park WM, Ramirez S, Boyden ES
  TITLE     Recording of cellular physiological histories along optically 
            readable self-assembling protein chains.
  JOURNAL   Nat Biotechnol. 2023 Jan 2. doi: 10.1038/s41587-022-01586-7.
  PUBMED    36593405
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2023 Jan 2. doi: 10.1038/s41587-022-01586-7."
COMMENT     Sequence Label: pAAV-UBC-FLEX-XRI-FLAG
FEATURES             Location/Qualifiers
     source          1..2376
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1246..1269
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgattgact atactgctgc tggtttcacc ctcctccaag gtgcacatct gtacgctcct
       61 gaagacagag gtatctgcga tgtgctggtt gccaacggta agatcatcgc tgtggcctcc
      121 aacattccta gcgacatcgt tccaaactgc accgttgtcg atctgagcgg acagatcctc
      181 tgtccaggat tcattgatca gcacgtgcac ctcattggag gaggaggcga agctggtcct
      241 actactagaa ctcccgaagt cgcactgtct cgcctcacag aggcaggcgt tacctccgtc
      301 gtgggactcc tgggtactga tagcatctct aggcaccctg agtctctgct ggcaaagacc
      361 agagccctca atgaggaggg tatctccgct tggatgctga ctggtgctta tcacgtccca
      421 tctcgcacca ttactggcag cgtggagaag gacgttgcaa tcatcgacag agtgatcggt
      481 gttaagtgcg ctatctccga ccacaggagc gctgctcccg acgtctatca cctggccaac
      541 atggcagccg agagcagagt cggtggtctg ctcggtggca agccaggagt taccgtgttc
      601 cacatgggtg actctaagaa ggcactgcaa cccatctatg acctgctgga gaactgtgac
      661 gtcccaatct ccaagctgct gcctacccac gtcaacagga acgtgcctct cttctaccaa
      721 gctctggagt tcgcaagaaa gggtggcacc atcgacatca cctctagcat tgatgaaccc
      781 gtcgctccag ctgagggaat cgcaagggct gtccaagcag gtatcccact ggcaagggtg
      841 acactcagct ctgatggcaa cggctcccag ccattcttcg atgatgaagg aaatctgact
      901 cacattggcg tggctggatt tgaaactctc ctggaaacag ttcaggttct ggttaaggac
      961 tacgacttca gcatttctga cgctctcaga ccactcacct ctagcgtcgc tggcttcctg
     1021 aacctgactg gtaaaggcga aatcctccct ggtaatgacg ctgatctgct ggtgatgaca
     1081 cccgaactgc gcattgagca ggtctatgca agaggaaagc tgatggttaa ggacggaaag
     1141 gcttgtgtca agggcacatt cgagactgct ggtggctctg gaggcaccgg agggtctgga
     1201 gggactggag gctctggagg tactggcggt tctggaggta ccggtgacta caaggacgat
     1261 gatgacaaag gatccggtaa gattgaagag ggcaaactgg ttatctggat caatggcgat
     1321 aagggctaca atggcctggc agaggtcggt aagaagttcg agaaagatac aggcatcaaa
     1381 gttactgtgg aacacccaga taagctggag gagaagttcc ctcaggtcgc agcaactggc
     1441 gacggtccag acatcatctt ctgggcacac gataggttcg gtggctatgc ccaatctggc
     1501 ctgctggctg agattactcc tgataaggct ttccaggaca agctgtatcc attcacctgg
     1561 gatgctgtga gatacaatgg caagctgatc gcatacccta ttgctgtgga agctctgagc
     1621 ctgatctaca acaaggacct gctgcctaat cctcctaaga catgggaaga aatccctgca
     1681 ctggacaagg aactgaaggc caaaggcaag tccgcactga tgttcaacct gcaggagcct
     1741 tactttacct ggccactgat tgctgccgac ggtggttacg ctttcaagta cgagaatggt
     1801 aagtacgaca tcaaagatgt gggtgtggac aatgctggtg ccaaagctgg tctgactttc
     1861 ctggtggatc tgatcaagaa caagcacatg aatgcagaca ctgactattc tatcgcagag
     1921 gctgctttca acaaaggcga aaccgcaatg actatcaatg gtccttgggc atggtctaac
     1981 atcgacacta gcaaagtcaa ctacggtgtc accgttctgc caaccttcaa gggtcagcca
     2041 agcaaacctt tcgttggcgt gctgagcgca ggtatcaacg ctgcctctcc taacaaagag
     2101 ctggctaagg agtttctgga gaactacctg ctgacagacg aaggtctgga ggcagtgaac
     2161 aaggacaagc cactgggtgc cgtggctctg aagagctacg aagaagaact ggccaaggac
     2221 cctcgcatcg ctgctacaat ggagaacgca cagaagggtg agatcatgcc aaacatccca
     2281 cagatgagcg cattctggta tgccgtgagg accgctgtta tcaacgcagc ttctggcaga
     2341 cagaccgtgg atgaagccct gaaagacgca cagacc
//
