LOCUS       sequence-345607-       10943 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A block for the AdenoBuilder genome assembly system. The insert 
            consolidates the regions present in pAd5-B6deltaE3 and pAd5-B7..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10943)
  AUTHORS   Jang Y, Bunz F
  TITLE     AdenoBuilder: A platform for the modular assembly of recombinant 
            adenoviruses.
  JOURNAL   STAR Protoc. 2022 Jan 20;3(1):101123. doi: 
            10.1016/j.xpro.2022.101123. eCollection 2022 Mar 18.
  PUBMED    35098167
REFERENCE   2  (bases 1 to 10943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "STAR 
            Protoc."; date: "2022-01-20"; volume: "3(1):101123. doi"; pages: " 
            10.1016/j.xpro.2022.101123. eCollection 2022 Mar 18"
COMMENT     Sequence Label: pAd5-B6/7deltaE3
FEATURES             Location/Qualifiers
     source          1..10943
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     305..324
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        305..323
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   379..481
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     promoter        complement(join(8775..8781,8782..8799,8800..8805))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    8820..8841
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8961..8978)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9132..9720)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9212..9231)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9891..10682,10683..10751))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10514..10533
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10752..10856)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10924..10942
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gcccctgcag ccgaattata ttatttttgc caaataattt ttaacaaaag ctctgaagtc
       61 ttcttcattt aaattcttag atgatacttc atctggaaaa ttgtcccaat tagtagcatc
      121 acgctgtgag taagttctaa accatttttt tattgttgta ttatctctaa tcttactact
      181 cgatgagttt tcggtattat ctctattttt aacttggagc aggttccatt cattgttttt
      241 ttcatcatag tgaataaaat caactgcttt aacacttgtg cctgaacacc atatccatcc
      301 ggcgtaatac gactcactat agggagagcg gccgccagat cttccggatg gctcgagttt
      361 ttcagcaaga tgttcgaaca tcatcaataa tataccttat tttggattga agccaatatg
      421 ataatgaggg ggtggagttt gtgacgtggc gcggggcgtg ggaacggggc gggtgacgta
      481 ggttttaggg cggagtaact tgtatgtgtt gggaattgta gttttcttaa aatgggaagt
      541 gacgtaacgt gggaaaacgg aagtgacgat ttgaggaagt tgtgggtttt ttggctttcg
      601 tttctgggcg taggttcgcg tgcggttttc tgggtgtttt ttgtggactt taaccgttac
      661 gtcatttttt agtcctatat atactcgctc tgcacttggc ccttttttac actgtgactg
      721 attgagctgg tgccgtgtcg agtggtgttt ttttaatagg ttttcttttt tactggtaag
      781 gctgactgtt atggctgccg ctgtggaagc gctgtatgtt gttctggagc gggagggtgc
      841 tattttgcct aggcaggagg gtttttcagg tgtttatgtg tttttctctc ctattaattt
      901 tgttatacct cctatggggg ctgtaatgtt gtctctacgc ctgcgggtat gtattccccc
      961 gggctatttc ggtcgctttt tagcactgac cgatgtgaat caacctgatg tgtttaccga
     1021 gtcttacatt atgactccgg acatgaccga ggagctgtcg gtggtgcttt ttaatcacgg
     1081 tgaccagttt ttttacggtc acgccggcat ggccgtagtc cgtcttatgc ttataagggt
     1141 tgtttttcct gttgtaagac aggcttctaa tgtttaaatg tttttttgtt attttatttt
     1201 gtgtttatgc agaaacccgc agacatgttt gagagaaaaa tggtgtcttt ttctgtggtg
     1261 gttccggagc ttacctgcct ttatctgcat gagcatgact acgatgtgct ttcttttttg
     1321 cgcgaggctt tgcctgattt tttgagcagc accttgcatt ttatatcgcc gcccatgcaa
     1381 caagcttaca tcggggctac gctggttagc atagctccga gtatgcgtgt cataatcagt
     1441 gtgggttctt ttgtcatggt tcctggcggg gaagtggccg cgctggtccg tgcagacctg
     1501 cacgattatg ttcagctggc cctgcgaagg gacctacggg atcgcggtat ttttgttaat
     1561 gttccgcttt tgaatcttat acaggtctgt gaggaacctg aatttttgca atcatgattc
     1621 gctgcttgag gctgaaggtg gagggcgctc tggagcagat ttttacaatg gccggactta
     1681 atattcggga tttgcttaga gatatattga gaaggtggcg agatgagaat tatttgggca
     1741 tggttgaagg tgctggaatg tttatagagg agattcaccc tgaagggttt agcctttacg
     1801 tccacttgga cgtgagggcc gtttgccttt tggaagccat tgtgcaacat cttacaaatg
     1861 ccattatctg ttctttggct gtagagtttg accacgccac cggaggggag cgcgttcact
     1921 taatagatct tcattttgag gttttggata atcttttgga ataaaaaaaa aaaacatggt
     1981 tcttccagct cttcccgctc ctcccgtgtg tgactcgcag aacgaatgtg taggttggct
     2041 gggtgtggct tattctgcgg tggtggatgt tatcagggca gcggcgcatg aaggagttta
     2101 catagaaccc gaagccaggg ggcgcctgga tgctttgaga gagtggatat actacaacta
     2161 ctacacagag cgatctaagc ggcgagaccg gagacgcaga tctgtttgtc acgcccgcac
     2221 ctggttttgc ttcaggaaat atgactacgt ccggcgttcc atttggcatg acactacgac
     2281 caacacgatc tcggttgtct cggcgcactc cgtacagtag ggatcgtcta cctccttttg
     2341 agacagaaac ccgcgctacc atactggagg atcatccgct gctgcccgaa tgtaacactt
     2401 tgacaatgca caacgtgagt tacgtgcgag gtcttccctg cagtgtggga tttacgctga
     2461 ttcaggaatg ggttgttccc tgggatatgg ttctaacgcg ggaggagctt gtaatcctga
     2521 ggaagtgtat gcacgtgtgc ctgtgttgtg ccaacattga tatcatgacg agcatgatga
     2581 tccatggtta cgagtcctgg gctctccact gtcattgttc cagtcccggt tccctgcagt
     2641 gtatagccgg cgggcaggtt ttggccagct ggtttaggat ggtggtggat ggcgccatgt
     2701 ttaatcagag gtttatatgg taccgggagg tggtgaatta caacatgcca aaagaggtaa
     2761 tgtttatgtc cagcgtgttt atgaggggtc gccacttaat ctacctgcgc ttgtggtatg
     2821 atggccacgt gggttctgtg gtccccgcca tgagctttgg atacagcgcc ttgcactgtg
     2881 ggattttgaa caatattgtg gtgctgtgct gcagttactg tgctgattta agtgagatca
     2941 gggtgcgctg ctgtgcccgg aggacaaggc gccttatgct gcgggcggtg cgaatcatcg
     3001 ctgaggagac cactgccatg ttgtattcct gcaggacgga gcggcggcgg cagcagttta
     3061 ttcgcgcgct gctgcagcac caccgcccta tcctgatgca cgattatgac tctaccccca
     3121 tgtaggcgtg gacttctcct tcgccgcccg ttaagcaacc gcaagttgga cagcagcctg
     3181 tggctcagca gctggacagc gacatgaact taagtgagct gcccggggag tttattaata
     3241 tcactgatga gcgtttggct cgacaggaaa ccgtgtggaa tataacacct aagaatatgt
     3301 ctgttaccca tgatatgatg ctttttaagg ccagccgggg agaaaggact gtgtactctg
     3361 tgtgttggga gggaggtggc aggttgaata ctagggttct gtgagtttga ttaaggtacg
     3421 gtgatctgta taagctatgt ggtggtgggg ctatactact gaatgaaaaa tgacttgaaa
     3481 ttttctgcaa ttgaaaaata aacacgttga aacataacac aaacgattct ttattcttgg
     3541 gcaatgtatg aaaaagtgta agaggatgtg gcaaatattt cattaatgta gttgtggcca
     3601 gaccagtccc atgaaaatga catagagtat gcacttggag ttgtgtctcc tgtttcctgt
     3661 gtaccgttta gtgtaatggt tagtgttaca ggtttagttt tgtctccgtt taagtaaact
     3721 tgactgacaa tgttactttt ggcagtttta ccgtgagatt ttggataagc tgataggtta
     3781 ggcataaatc caacagcgtt tgtataggct gtgccttcag taagatctcc atttctaaag
     3841 ttccaatatt ctgggtccag gaaggaattg tttagtagca ctccattttc gtcaaatctt
     3901 ataataagat gagcactttg aactgttcca gatattggag ccaaactgcc tttaacagcc
     3961 aaaactgaaa ctgtagcaag tatttgactg ccacattttg ttaagaccaa agtgagttta
     4021 gcatctttct ctgcatttag tctacagtta ggagatggag ctggtgtggt ccacaaagtt
     4081 agcttatcat tatttttgtt tcctactgta atggcacctg tgctgtcaaa actaaggcca
     4141 gttcctagtt taggaaccat agccttgttt gaatcaaatt ctaggccatg gccaattttt
     4201 gttttgaggg gatttgtgtt tggtgcatta ggtgaaccaa attcaagccc atctcctgca
     4261 ttaatggcta tggctgtagc gtcaaacatc aaccccttgg cagtgcttag gttaacctca
     4321 agctttttgg aattgtttga agctgtaaac aagtaaaggc ctttgttgta gttaatatcc
     4381 aagttgtggg ctgagtttat aaaaagaggg ccctgtccta gtcttagatt tagttggttt
     4441 tgagcatcaa acggataact aacatcaagt ataaggcgtc tgttttgaga atcaatcctt
     4501 agtcctcctg ctacattaag ttgcatattg ccttgtgaat caaaacccaa ggctccagta
     4561 actttagttt gcaaggaagt attattaata gtcacacctg gaccagttgc tacggtcaaa
     4621 gtgtttaggt cgtctgttac atgcaaagga gccccgtact ttagtcctag ttttccattt
     4681 tgtgtataaa tgggctcttt caagtcaatg cccaagctac cagtggcagt agttagaggg
     4741 ggtgaggcag tgatagtaag ggtactgcta tcggtggtgg tgagggggcc tgatgtttgc
     4801 agggctagct ttccttctga cactgtgagg ggtccttggg tggcaatgct aagtttggag
     4861 tcgtgcacgg ttagcggggc ctgtgattgc atggtgagtg tgttgcccgc gaccattaga
     4921 ggtgcggcgg cagccacagt tagggcttct gaggtaactg tgaggggtgc agatatttcc
     4981 aggtttatgt ttgacttggt ttttttgaga ggtgggctca cagtggttac attttgggag
     5041 gtaaggttgc cggcctcgtc cagagagagg ccgttgccca ttttgagcgc aagcatgcca
     5101 ttggaggtaa ctagaggttc ggataggcgc aaagagagta ccccaggggg actctcttga
     5161 aacccattgg gggatacaaa gggaggagta agaaaaggca cagttggagg accggtttcc
     5221 gtgtcatatg gatacacggg gttgaaggta tcttcagacg gtcttgcgcg cttcatctgc
     5281 aacaacatga agatagtggg tgcggatgga caggaacagg aggaaactga cattccattt
     5341 agattgtgga gaaagtttgc agccaggagg aagctgcaat accagagctg ggaggagggc
     5401 aaggaggtgc tgctgaataa actggacaga aatttgctaa ctgattttaa gtaagtgatg
     5461 ctttattatt tttttttatt agttaaaggg aataagatct ttgagaccgt cgacatatgg
     5521 atccttcagt cgtagccgtc cgccgagtcc tttaccgcgt caaagttagg aataaattga
     5581 tccggatagt ggccgggagg tcccgagaag gggttaaagt agaccgatgg cacaaactcc
     5641 tcaataaatt gcagagttcc aatgcctcca gagcgcggct cagaggacga ggtctgcaga
     5701 gttaggattg cctgacgagg cgtgaatgaa gagcggccgg cgccgccgat ctgaaatgtc
     5761 ccgtccggac ggagaccaag cgaggagctc accgactcgt cgttgagctg aatacctcgc
     5821 cctctgattg tcaggtgagt tataccctgc ccgggcgacc gcaccctgtg acgaaagccg
     5881 cccgcaagct gcgcccctga gttagtcatc tgaacttcgg cctgggcgtc tctgggaagt
     5941 accacagtgg tgggagcggg actttcctgg tacaccaggg cagcgggcca actacgggga
     6001 ttaaggttat tacgaggtgt ggtggtaata gccgcctgtt ccaggagaat tcggtttcgg
     6061 tgggcgcgta ttccgttgac ccgggatatc atgtggggtc ccgcgctcat gtagtttatt
     6121 cgggttgagt agtcttgggc agctccagcc gcaagtccca tttgtggctg gtaactccac
     6181 atgtagggcg tgggaatttc cttgctcata atggcgctga caacaggtgc tggcgccggg
     6241 tgtggccgct ggagatgacg tagttttcgc gcttaaattt gagaaagggc gcgaaactag
     6301 tccttaagag tcagcgcgca gtatttactg aagagagcct ccgcgtcttc cagcgtgcgc
     6361 cgaagctgat cttcgctttt gtgatacagg cagctgcggg tgagggatcg cagagacctg
     6421 ttttttattt tcagctcttg ttcttggccc ctgctctgtt gaaatatagc atacagagtg
     6481 ggaaaaatcc tgtttctaag ctcgcgggtc gatacgggtt cgttgggcgc cagacgcagc
     6541 gctcctcctc ctgctgctgc cgccgctgtg gatttcttgg gctttgtcag agtcttgcta
     6601 tccggtcgcc tttgcttctg tgtggccgct gctgttgctg ccgctgccgc cggtgcagta
     6661 tgggctgtag agatgacggt agtaatgcag gatgttacgg gggaaggcca cgccgtgatg
     6721 gtagagaaga aagcggcggg cgaaggagat gttgccccca cagtcttgca agcaagcaac
     6781 tatggcgttc ttgtgcccgc gccatgagcg gtagccttgg cgctgttgtt gctcttgggc
     6841 taacggcggc ggctgcttgg acttaccggc cctggttcca gtggtgtccc atctacggtt
     6901 gggtcggcga acgggcagtg ccggcggcgc ctgaggagcg gaggttgtag ccatgctgga
     6961 accggttgcc gatttctggg gcgccggcga ggggaatgcg accgagggtg acggtgtttc
     7021 gtctgacacc tcttcgacct cggaagcttc ctcgtctagg ctctcccagt cttccatcat
     7081 gtcctcctcc tcctcgtcca aaacctcctc tgcctgactg tcccagtatt cctcctcgtc
     7141 cgtgggtggc ggcggcagct gcagcttctt tttgggtgcc atcctgggaa gcaagggccc
     7201 gcggctgctg ctgatagggc tgcggcggcg gggggattgg gttgagctcc tcgccggact
     7261 gggggtccaa gtaaaccccc cgtccctttc gtagcagaaa ctcttggcgg gctttgttga
     7321 tggcttgcaa ttggccaaga atgtggccct gggtaatgac gcaggcggta agctccgcat
     7381 taggcgggcg ggattggtct tcgtagaacc taatctcgtg ggcgtggtag tcctcaggta
     7441 caaatttgcg aaggtaagcc gacgtccaca gccccggagt gagtttcaac cccggagccg
     7501 cggacttttc gtcaggcgag ggaccctgca gctcaaaggt accgataatt tgactttcgt
     7561 taagcagctg cgaattgcaa accagggagc ggtgcggggt gcataggttg cagcgacagt
     7621 gacactccag tagaccgtca ccgctcacgt cttccattat gtcagagtgg taggcaaggt
     7681 agttggctag ctgcagaagg tagcagtggc cccaaagcgg cggagggcat tcgcggtact
     7741 taatgggcac aaagtcgcta ggaagtgcac agcaggtggc gggcaagatt cctgagcgct
     7801 ctaggataaa gttcctaaag ttctgcaaca tgctttgact ggtgaagtct ggcagaccct
     7861 gttgcagggt tttaagcagg cgttcgggga aaatgatgtc cgccaggtgc gcggccacgg
     7921 agcgctcgtt gaaggccgtc cataggtcct tcaagttttg ctttagcagt ttctgcagct
     7981 ccttgaggtt gcactcctcc aagcactgct gccaaacgcc catggccgtc tgccaggtgt
     8041 agcatagaaa taagtaaacg cagtcgcgga cgtagtcgcg gcgcgcctcg cccttgagcg
     8101 tggaatgaag cacgttttgc ccaaggcggt tttcgtgcaa aattccaagg taggagacca
     8161 ggttgcagag ctccacgttg gagatcttgc aggcctggcg tacgtagccc tgtcgaaagg
     8221 tgtagtgcaa tgtttcctct agcttgcgct gcatctccgg gtcagcaaag aaccgctgca
     8281 tgcactcaag ctccacggta acgagcactg cggccatcat tagtttgcgt cgcttcgaag
     8341 atctttctag aagatctcct acaatattct cagctgccat ggaaaatcga tgttcttctt
     8401 ttattctctc aagattttca ggctgtatat taaaacttat attaagaact atgctaacca
     8461 cctcatcagg aaccgttgta ggtggcgtgg gttttcttgg caatcgactc tcatgaaaac
     8521 tacgagctaa atattcaata tgttcctctt gaccaacttt attctgcatt ttttttgaac
     8581 gaggtttaga gcaagcttca ggaaactgag acaggaattt tattaaaaat ttaaattttg
     8641 aagaaagttc agggttaata gcatccattt tttgctttgc aagttcctca gcattcttaa
     8701 caaaagacgt ctcttttgac atgtttaaag tttaaacctc ctgtgtgaaa ttattatccg
     8761 ctcataattc cacacattat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     8821 tgagtgagct aactcacatt aattgcgttg cgctcactgc caattgcttt ccagtcggga
     8881 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     8941 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     9001 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     9061 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     9121 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     9181 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     9241 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     9301 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     9361 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     9421 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     9481 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     9541 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     9601 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     9661 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     9721 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     9781 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     9841 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     9901 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     9961 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
    10021 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
    10081 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
    10141 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
    10201 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
    10261 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
    10321 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
    10381 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
    10441 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
    10501 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
    10561 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
    10621 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
    10681 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
    10741 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
    10801 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
    10861 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
    10921 aaaaataggc gtatcacgag gcc
//
