LOCUS       sequence-345661-        5221 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5221)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5221)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_1124:Dendra
FEATURES             Location/Qualifiers
     source          1..5221
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     963..982
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        963..981
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2082..2105
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2362..3162
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3287..3875
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3776..3795
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atcctggcgt ggacgagcct ccggttcgga gagctgatcg agcttcgccg caaggacatc
       61 gtggacgacg gcatgacgat gaagctccgg gtgcgccgtg gcgcttcccg cgtggggaac
      121 aagatcgtcg ttggcaacgc caagaccgtc cggtcgaagc gtcctgtgac ggttccgcct
      181 cacgtcgcgg agatgatccg agcgcacatg aaggaccgta cgaagatgaa caagggcccc
      241 gaggcattcc tggtgaccac gacgcagggc aaccggctgt cgaagtccgc gttcaccaag
      301 tcgctgaagc gtggctacgc caagatcggt cggccggaac tccgcatcca cgacctccgc
      361 gctgtcggcg ctacgttcgc cgctcaggca ggtgcgacga ccaaggagct gatggcccgt
      421 ctcggtcaca cgactcctag gatggcgatg aagtaccaga tggcgtctga ggcccgcgac
      481 gaggctatcg ctgaggcgat gtccaagctg gccaagacct cctgaaacgc aaaaagcccc
      541 cctcccaagg acactgagtc ctaaagaggg gggtttcttg tcagtacgcg aagaaccacg
      601 cctggccgcg agcgccagca ccgccgctct gtgcggagac ctgggcacca gccccgccgc
      661 cgccaggagc attgccgttc ccgccagaaa tctagacggt gaccacaacg cgcccgcttt
      721 gatcggggac gtcgaaatat tggatcgtcg gcaccgtcac ggccgtggga ggcggcacga
      781 tccgcgacgt gatgatcggc cgcatcccca cggtgctgcg cagtgagctc tacgccatcc
      841 cggcgttgat ctgtgcgttc gcacgcacag gcccggtgtg agaagggtct ctgacgagcg
      901 ggagaactcc ctatcagtga tagagtttgt cctccctatc agtgatagat aggcttaagg
      961 agtaatacga ctcactatag ggagaaggag gccttatgac atacgtgatc ggccggccgt
     1021 gcgtggacgt caaagaccgc gcatgcgtgg atgagtgccc ggtcgactgc atctacgagg
     1081 gcgcgcggat gctctacatc caccccgacg aatgtgtcga ctgtggcgcg tgtgaacccg
     1141 tgtgcccggt ggaggcgatc tactacgagg acgacctgcc cgaggatctg cagccgtacc
     1201 aggaggagaa cgcgaagttc ttcaccgatg tcctgcccgg gcgtgcccaa ccgctcggat
     1261 caccgggcgg tgccgcgaaa ctcggtgtcg tggacgccga tacgcccatg gttgcggagc
     1321 taccgccgca gggggataag cttggtgcgg ccgcgggcaa cacgcctggc atcaatctga
     1381 tcaaggagga catgcgtgtc aaggtgcata tggagggcaa cgtgaacggt cacgccttcg
     1441 tgatcgaggg ggagggtaag ggtaagcctt acgaagggac ccagaccgcc aacctcaccg
     1501 tcaaggaggg cgcccctctc ccattctcgt acgatatcct cactaccgcg gtgcattacg
     1561 ggaaccgcgt gttcaccaag taccccgagg atatccctga ctacttcaag cagagcttcc
     1621 cggagggata ctcctgggag cggacgatga ccttcgaaga caagggtatc tgtacgatcc
     1681 gcagcgacat ctcgctggaa ggggactgtt tcttccagaa cgtccgtttc aaggggacca
     1741 actttccgcc gaacggcccg gtcatgcaga agaagaccct caagtgggag ccgagcacgg
     1801 agaagctgca cgttcgggac ggcctcctgg tcggcaacat caacatggcg ctgctgctgg
     1861 aaggcggcgg ccactaccta tgcgacttca agaccacgta caaagcgaag aaggtcgtcc
     1921 agctgcccga cgcgcacttc gtggaccacc gcatcgaaat cctggggaac gattcggact
     1981 acaacaaggt caagttgtac gagcacgctg tggcacgcta tagcccgctc ccgtcgcagg
     2041 tctggttcga aggcggcggc ggggagaacc tgtatttcca ggactacaag gacgacgacg
     2101 acaagtgatt taaataaccc cttggggcct ctaaacgggt cttgaggggt tttttgttaa
     2161 ctagcgtacg atcgaattcc tgcagctcac ggtaactgat gccgtatttg cagtaccagc
     2221 gtacggccca cagaatgatg tcacgctgaa aatgccggcc tttgaatggg ttcatgtgca
     2281 gctccatcag caaaagggga tgataagttt atcaccaccg actatttgca acagtgccgt
     2341 tgatcgtgct atgatcgact gatgtcatca gcggtggagt gcaatgtcgt gcaatacgaa
     2401 tggcgaaaag ccgagctcat cggtcagctt ctcaaccttg gggttacccc cggcggtgtg
     2461 ctgctggtcc acagctcctt ccgtagcgtc cggcccctcg aagatgggcc acttggactg
     2521 atcgaggccc tgcgtgctac gctgggtccg ggagggacgc tcgtcatgcc ctcgtggtca
     2581 ggtctggacg acgagccgtt cgatcctgcc acgtcgcccg ttacaccgga ccttggagtt
     2641 gtctctgaca cattctggcg cctgccaaat gtaaagcgca gcgcccatcc atttgccttt
     2701 gcggcagcgg ggccacaggc agagcagatc atctctgatc cattgcccct gccaccttac
     2761 tcgcctgcaa gcccggtcgc ccgtgtccat gaactcgatg ggcaggtact tctcctcggc
     2821 gtgggacacg atgccaacac gacgctgcat cttgccgagt tgatggcaaa ggttccctat
     2881 ggggtgccga gacactgcac cattcttcag gatggcaagt tggtacgcgt cgattatctc
     2941 gagaatgacc actgctgtga gcgctttgcc ttggcggaca ggtggctcaa ggagaagagc
     3001 cttcagaagg aaggtccagt cggtcatgcc tttgctcggt tgatccgctc ccgcgacatt
     3061 gtggcgacag ccctgggtca actgggccga gatccgttga tcttcctgca tccgccagag
     3121 gcgggatgcg aagaatgcga tgccgctcgc cagtcgattg gctgagctca tgagcggaga
     3181 acgagatgac gttggagggg caaggtcgcg ctgattgctg gggcaacacg ggggatccac
     3241 tagttccatg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     3301 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     3361 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     3421 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     3481 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     3541 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     3601 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     3661 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaggggaga aaggcggaca
     3721 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     3781 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     3841 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     3901 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     3961 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     4021 ccgagcgcaa cgcgtgcggc cgcggtaccc ggggatcctc tagagtcgac caccaagggc
     4081 accatctctg cttgggccac cccgttggcc gcagccagct cgctgagagc cgtgaacgac
     4141 agggcgaacg ccagcccgcc gacggcgagg gttccgaccg ctgcaactcc cggtgcaacc
     4201 ttgtcccggt ctattctctt cactgcacca gctccaatct ggtgtgaatg cccctcgtct
     4261 gttcgcgcag gcggggggct ctattcgttt gtcagcatcg aaagtagcca gatcagggat
     4321 gcgttgcaac cgcgtatgcc caggtcagaa gagtcgcaca agagttgcag acccctggaa
     4381 agaaaaatgg ccagagggcg aaaacaccct ctgaccagcg gagcgggcga cgggaatcga
     4441 acccgcgtag ctagtttgga agaatgggtg tctgccgacc acatatgggc cggtcaagat
     4501 aggtttttac cccctctcgg ctgcatcctc taagtggaaa gaaattgcag gtcgtagaag
     4561 cgcgttgaag cctgagagtt gcacaggagt tgcaacccgg tagccttgtt cacgacgaga
     4621 ggagacctag ttggcacgtc gcggatgggg atcgctgaag actcagcgca gcgggaggat
     4681 ccaagcctca tacgtcaacc cgcaggacgg tgtgaggtac tacgcgctgc agacctacga
     4741 caacaagatg gacgccgaag cctggctcgc gggcgagaag cggctcatcg agatggagac
     4801 ctggacccct ccacaggacc gggcgaagaa ggcagccgcc agcgccatca cgctggagga
     4861 gtacacccgg aagtggctcg tggagcgcga cctcgcagac ggcaccaggg atctgtacag
     4921 cgggcacgcg gagcgccgca tctacccggt gctaggtgaa gtggcggtca cagagatgac
     4981 gccagctctg gtgcgtgcgt ggtgggccgg gatgggtagg aagcacccga ctgcccgccg
     5041 gcatgcctac aacgtcctcc gggcggtgat gaacacagcg gtcgaggaca agctgatcgc
     5101 agagaacccg tgccggatcg agcagaaggc agccgatgag cgcgacgtag aggcgctgac
     5161 gcctgaggag ctggacatcg tcgccgctga gatcttcgag cactaccgga tcgcggcata
     5221 c
//
