LOCUS       sequence-345672-        5741 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5741)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_1011:Dendra
FEATURES             Location/Qualifiers
     source          1..5741
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     769..788
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        769..787
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2408..2431
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2688..3488
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3613..4201
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4102..4121
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gatccgagcg cacatgaagg accgtacgaa gatgaacaag ggccccgagg cattcctggt
       61 gaccacgacg cagggcaacc ggctgtcgaa gtccgcgttc accaagtcgc tgaagcgtgg
      121 ctacgccaag atcggtcggc cggaactccg catccacgac ctccgcgctg tcggcgctac
      181 gttcgccgct caggcaggtg cgacgaccaa ggagctgatg gcccgtctcg gtcacacgac
      241 tcctaggatg gcgatgaagt accagatggc gtctgaggcc cgcgacgagg ctatcgctga
      301 ggcgatgtcc aagctggcca agacctcctg aaacgcaaaa agcccccctc ccaaggacac
      361 tgagtcctaa agaggggggt ttcttgtcag tacgcgaaga accacgcctg gccgcgagcg
      421 ccagcaccgc cgctctgtgc ggagacctgg gcaccagccc cgccgccgcc aggagcattg
      481 ccgttcccgc cagaaatcta gacggtgacc acaacgcgcc cgctttgatc ggggacgtcg
      541 aaatattgga tcgtcggcac cgtcacggcc gtgggaggcg gcacgatccg cgacgtgatg
      601 atcggccgca tccccacggt gctgcgcagt gagctctacg ccatcccggc gttgatctgt
      661 gcgttcgcac gcacaggccc ggtgtgagaa gggtctctga cgagcgggag aactccctat
      721 cagtgataga gtttgtcctc cctatcagtg atagataggc ttaaggagta atacgactca
      781 ctatagggag aaggaggcca ttatggcgca ggggagcgac ttcgcgggca agagatgttt
      841 cgtgaccggg gcggccagcg gcatcggccg cgccacggcg ctggcgctcg ccgcggcggg
      901 cgccgagctc tacctgaccg accgcgacgc cgacgggctt gtgcagaccg tcgccgatgc
      961 gcgcgcgctc ggcgccgagg tgcctgcgca ccgcgcgctc gacatctccg actacgacca
     1021 agtgcgcgag ttcgccaagg acatccacgc cgcgcacggg tcgatggacg tcgtgatgaa
     1081 catcgcgggc gtgtccgcgt ggggcaccgt cgaccggctc agtcaccagc agtggcggtc
     1141 gatggtcgac atcaacctca tggggcccat ccacgtcatc gaggagttca tcccgccgat
     1201 gatcgaggcg cgccgcggcg ggcacctggt caacgtgtcc tcggcggccg gtctggtcgc
     1261 cctgccgtgg cacggtgcct acagcgcaag caaattcggc ctgcgcggcg tgagcgaggt
     1321 gctgcgattc gacctggcgc gacaccgcat cggggtgtcg gtggtggtgc ccggcgccgt
     1381 gaagaccggc ctggtgcaga ccgtcgagat cgccggcgtc gaccgcgagg accccgacgt
     1441 caagaagtgg gtggatcgct ttgccggaca tgcgatctca cctgagaagg ccgccgcgaa
     1501 gatcctggcg ggggtacgac gcaaccggtt tttgatctac acctcggccg acatccgcgc
     1561 gctgtacacg ttcaagcgcg tggcctggtg gccctacagc gtggcgatgc gccaggtgaa
     1621 cgtgctgttc acccgcgctc tgcgctccgg cacatcgcca cgcaagcttg gtgcggccgc
     1681 gggcaacacg cctggcatca atctgatcaa ggaggacatg cgtgtcaagg tgcatatgga
     1741 gggcaacgtg aacggtcacg ccttcgtgat cgagggggag ggtaagggta agccttacga
     1801 agggacccag accgccaacc tcaccgtcaa ggagggcgcc cctctcccat tctcgtacga
     1861 tatcctcact accgcggtgc attacgggaa ccgcgtgttc accaagtacc ccgaggatat
     1921 ccctgactac ttcaagcaga gcttcccgga gggatactcc tgggagcgga cgatgacctt
     1981 cgaagacaag ggtatctgta cgatccgcag cgacatctcg ctggaagggg actgtttctt
     2041 ccagaacgtc cgtttcaagg ggaccaactt tccgccgaac ggcccggtca tgcagaagaa
     2101 gaccctcaag tgggagccga gcacggagaa gctgcacgtt cgggacggcc tcctggtcgg
     2161 caacatcaac atggcgctgc tgctggaagg cggcggccac tacctatgcg acttcaagac
     2221 cacgtacaaa gcgaagaagg tcgtccagct gcccgacgcg cacttcgtgg accaccgcat
     2281 cgaaatcctg gggaacgatt cggactacaa caaggtcaag ttgtacgagc acgctgtggc
     2341 acgctatagc ccgctcccgt cgcaggtctg gttcgaaggc ggcggcgggg agaacctgta
     2401 tttccaggac tacaaggacg acgacgacaa gtgatttaaa taaccccttg gggcctctaa
     2461 acgggtcttg aggggttttt tgttaactag cgtacgatcg aattcctgca gctcacggta
     2521 actgatgccg tatttgcagt accagcgtac ggcccacaga atgatgtcac gctgaaaatg
     2581 ccggcctttg aatgggttca tgtgcagctc catcagcaaa aggggatgat aagtttatca
     2641 ccaccgacta tttgcaacag tgccgttgat cgtgctatga tcgactgatg tcatcagcgg
     2701 tggagtgcaa tgtcgtgcaa tacgaatggc gaaaagccga gctcatcggt cagcttctca
     2761 accttggggt tacccccggc ggtgtgctgc tggtccacag ctccttccgt agcgtccggc
     2821 ccctcgaaga tgggccactt ggactgatcg aggccctgcg tgctacgctg ggtccgggag
     2881 ggacgctcgt catgccctcg tggtcaggtc tggacgacga gccgttcgat cctgccacgt
     2941 cgcccgttac accggacctt ggagttgtct ctgacacatt ctggcgcctg ccaaatgtaa
     3001 agcgcagcgc ccatccattt gcctttgcgg cagcggggcc acaggcagag cagatcatct
     3061 ctgatccatt gcccctgcca ccttactcgc ctgcaagccc ggtcgcccgt gtccatgaac
     3121 tcgatgggca ggtacttctc ctcggcgtgg gacacgatgc caacacgacg ctgcatcttg
     3181 ccgagttgat ggcaaaggtt ccctatgggg tgccgagaca ctgcaccatt cttcaggatg
     3241 gcaagttggt acgcgtcgat tatctcgaga atgaccactg ctgtgagcgc tttgccttgg
     3301 cggacaggtg gctcaaggag aagagccttc agaaggaagg tccagtcggt catgcctttg
     3361 ctcggttgat ccgctcccgc gacattgtgg cgacagccct gggtcaactg ggccgagatc
     3421 cgttgatctt cctgcatccg ccagaggcgg gatgcgaaga atgcgatgcc gctcgccagt
     3481 cgattggctg agctcatgag cggagaacga gatgacgttg gaggggcaag gtcgcgctga
     3541 ttgctggggc aacacggggg atccactagt tccatgagcg tcagaccccg tagaaaagat
     3601 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     3661 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     3721 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
     3781 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     3841 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     3901 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     3961 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     4021 gcttcccgag gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     4081 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     4141 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     4201 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     4261 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     4321 tgataccgct cgccgcagcc gaacgaccga gcgcaacgcg tgcggccgcg gtacccgggg
     4381 atcctctaga gtcgaccacc aagggcacca tctctgcttg ggccaccccg ttggccgcag
     4441 ccagctcgct gagagccgtg aacgacaggg cgaacgccag cccgccgacg gcgagggttc
     4501 cgaccgctgc aactcccggt gcaaccttgt cccggtctat tctcttcact gcaccagctc
     4561 caatctggtg tgaatgcccc tcgtctgttc gcgcaggcgg ggggctctat tcgtttgtca
     4621 gcatcgaaag tagccagatc agggatgcgt tgcaaccgcg tatgcccagg tcagaagagt
     4681 cgcacaagag ttgcagaccc ctggaaagaa aaatggccag agggcgaaaa caccctctga
     4741 ccagcggagc gggcgacggg aatcgaaccc gcgtagctag tttggaagaa tgggtgtctg
     4801 ccgaccacat atgggccggt caagataggt ttttaccccc tctcggctgc atcctctaag
     4861 tggaaagaaa ttgcaggtcg tagaagcgcg ttgaagcctg agagttgcac aggagttgca
     4921 acccggtagc cttgttcacg acgagaggag acctagttgg cacgtcgcgg atggggatcg
     4981 ctgaagactc agcgcagcgg gaggatccaa gcctcatacg tcaacccgca ggacggtgtg
     5041 aggtactacg cgctgcagac ctacgacaac aagatggacg ccgaagcctg gctcgcgggc
     5101 gagaagcggc tcatcgagat ggagacctgg acccctccac aggaccgggc gaagaaggca
     5161 gccgccagcg ccatcacgct ggaggagtac acccggaagt ggctcgtgga gcgcgacctc
     5221 gcagacggca ccagggatct gtacagcggg cacgcggagc gccgcatcta cccggtgcta
     5281 ggtgaagtgg cggtcacaga gatgacgcca gctctggtgc gtgcgtggtg ggccgggatg
     5341 ggtaggaagc acccgactgc ccgccggcat gcctacaacg tcctccgggc ggtgatgaac
     5401 acagcggtcg aggacaagct gatcgcagag aacccgtgcc ggatcgagca gaaggcagcc
     5461 gatgagcgcg acgtagaggc gctgacgcct gaggagctgg acatcgtcgc cgctgagatc
     5521 ttcgagcact accggatcgc ggcatacatc ctggcgtgga cgagcctccg gttcggagag
     5581 ctgatcgagc ttcgccgcaa ggacatcgtg gacgacggca tgacgatgaa gctccgggtg
     5641 cgccgtggcg cttcccgcgt ggggaacaag atcgtcgttg gcaacgccaa gaccgtccgg
     5701 tcgaagcgtc ctgtgacggt tccgcctcac gtcgcggaga t
//
