LOCUS       sequence-345718-       10322 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of orthogonal Mb tRNA and Acd82 tRNA synthetase for 
            incorporation of acridone in eukaryotic cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10322)
  AUTHORS   Jones CM, Robkis DM, Blizzard RJ, Munari M, Venkatesh Y, Mihaila TS,
            Eddins AJ, Mehl RA, Zagotta WN, Gordon SE, Petersson EJ
  TITLE     Genetic encoding of a highly photostable, long lifetime fluorescent 
            amino acid for imaging in mammalian cells.
  JOURNAL   Chem Sci. 2021 Aug 3;12(36):11955-11964. doi: 10.1039/d1sc01914g. 
            eCollection 2021 Sep 22.
  PUBMED    34976337
REFERENCE   2  (bases 1 to 10322)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1039/d1sc01914g"; journalName: "Chem Sci"; date: "2021-08-3- 3";
            volume: "12"; issue: "36"; pages: "11955-11964"
COMMENT     Sequence Label: pAcBac1.tR4-AcdRS82
FEATURES             Location/Qualifiers
     source          1..10322
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(112..566)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(249..270)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     460..479
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     mobile_element  750..915
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     promoter        complement(1008..1102)
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     protein_bind    1008..1026
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1036..1054
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(1179..1427)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     polyA_signal    1460..1594
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1484..1503)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1538..1557
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(join(1740..3227,3228..3275))
                     /codon_start=1
                     /product="vesicular stomatitis virus G glycoprotein"
                     /label=VSV-G
                     /note="Indiana strain"
                     /translation="MKCLLYLAFLFIGVNCKFTIVFPHNQKGNWKNVPSNYHYCPSSSD
                     LNWHNDLIGTALQVKMPKSHKAIQADGWMCHASKWVTTCDFRWYGPKYITHSIRSFTPS
                     VEQCKESIEQTKQGTWLNPGFPPQSCGYATVTDAEAVIVQVTPHHVLVDEYTGEWVDSQ
                     FINGKCSNYICPTVHNSTTWHSDYKVKGLCDSNLISMDITFFSEDGELSSLGKEGTGFR
                     SNYFAYETGGKACKMQYCKHWGVRLPSGVWFEMADKDLFAAARFPECPEGSSISAPSQT
                     SVDVSLIQDVERILDYSLCQETWSKIRAGLPISPVDLSYLAPKNPGTGPAFTIINGTLK
                     YFETRYIRVDIAAPILSRMVGMISGTTTERELWDDWAPYEDVEIGPNGVLRTSSGYKFP
                     LYMIGHGMLDSDLHLSSKAQVFEHPHIQDAASQLPDDESLFFGDTGLSKNPIELVEGWF
                     SSWKSSIASFFFIIGLIIGLFLVLRVGIHLCIKLKHTKKRQIYTDIEMNRLGK"
     promoter        complement(3334..3425)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     complement(3392..3409)
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     enhancer        3520..3899
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3900..4103
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4054..4074
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4148..4167
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4148..4166
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      4186..4195
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4216..4242
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     misc_feature    5525..6113
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5578..5598)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5996..6007)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6162..6179)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6168..6392
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        6429..6677
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     promoter        6754..6848
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     protein_bind    6802..6820
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6830..6848
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        join(7338..7343,7344..7360,7361..7366)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             7555..8088
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     mobile_element  8155..8379
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     primer_bind     complement(8513..8530)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8684..9272)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8764..8783)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9443..10234,10235..10303))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10066..10085
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(10304..10322,1..86))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
       61 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc taaattgtaa
      121 gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
      181 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
      241 gtgttgttcc agtttggaac aagagtccac tattaagaac gtggactcca acgtcaaagg
      301 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt
      361 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag
      421 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc
      481 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc
      541 gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc aggctgcaaa taagcgttga
      601 tattcagtca attacaaaca ttaataacga agagatgaca gaaaaatttt cattctgtga
      661 cagagaaaaa gtagccgaag atgacggttt gtcacatgga gttggcagga tgtttgatta
      721 aaaacataac aggaagaaaa atgccccgct gtgggcggac aaaatagttg ggaactggga
      781 ggggtggaaa tggagttttt aaggattatt tagggaagag tgacaaaata gatgggaact
      841 gggtgtagcg tcgtaagcta atacgaaaat taaaaatgac aaaatagttt ggaactagat
      901 ttcacttatc tggttcggat ctcctagcaa aaaacggggg gtgcgggagt ttcacccgcc
      961 tgcacggatt tagagtccgt gtgatctatt cgatccaccc cccggtgtct ctatcactga
     1021 tagggaactt ataagtctct atcactgata gggatttcac gtttatggtg atttcccaga
     1081 acacatagcg acatgcaaat attaaaaaac ggaaaccccg ggaatctaac ccggctgaac
     1141 ggatttagag tccgttcgat ctacatgatc aggtttccgg tgtttcgtcc tttccacaag
     1201 atatataaag ccaagaaatc gaaatacttt caagttacgg taagcatatg atagtccatt
     1261 ttaaaacata attttaaaac tgcaaactac ccaagaaatt attactttct acgtcacgta
     1321 ttttgtacta atatctttgt gtttacagtc aaattaattc taattatctc tctaacagcc
     1381 ttgtatcgta tatgcaaata tgaaggaatc atgggaaata ggccctcttc ctgcccgacc
     1441 taggctcaag cagtgatcag atccagacat gataagatac attgatgagt ttggacaaac
     1501 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
     1561 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
     1621 gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg
     1681 tggtatggct gattatgatc ctctagtact tctcgacaag cttggccggc ctggccttat
     1741 cactttccaa gtcggttcat ctctatgtct gtataaatct gtcttttctt ggtgtgcttt
     1801 aatttaatgc aaagatggat accaactcgg agaaccaaga atagtccaat gattaaccct
     1861 atgataaaga aaaaagaggc aatagagctt ttccaactac tgaaccaacc ttctacaagc
     1921 tcgattggat ttttggatag cccagtatca ccaaaaaata aactctcatc atcaggaagt
     1981 tgcgaagcag cgtcttgaat gtgaggatgt tcgaacacct gagcctttga gctaagatga
     2041 agatcggagt ccaacatacc atgtccaatc atgtataaag gaaacttata tcctgaactg
     2101 gtcctcagaa ctccattggg tccaatttcc acgtcttcat atggtgccca gtcatcccac
     2161 agttcccttt ctgtggtagt tccactgatc attccgacca ttcttgagag gattggagca
     2221 gcaatatcga ctctgatgta tctggtctca aagtatttta gggtaccatt gattatggtg
     2281 aaagcaggac cggttcctgg gtttttagga gcaagatagc tgagatccac tggagagatt
     2341 ggaagacccg ctctgatttt gctccaggtt tcttggcaga gggaataatc caagatcctc
     2401 tcaacgtcct gaattagact tacatccact gaggtctgag atggagcaga gatacttgac
     2461 ccttctgggc attcagggaa tctggctgca gcaaagagat ccttatcagc catctcgaac
     2521 cagacacctg atgggagtct gactccccaa tgcttgcagt attgcatttt gcaggccttg
     2581 cctccagttt cataagcaaa gtagttactt ctgaaccctg tgccctcctt tcccagggat
     2641 gatagctctc cgtcctctga gaagaaggtg atgtccatgg aaatgaggtt agaatcacat
     2701 agccctttga ccttatagtc agaatgccag gttgtagagt tatggacagt ggggcatatg
     2761 taattgctgc attttccgtt gatgaactgt gaatcaaccc attctcctgt gtattcatca
     2821 accagcacat ggtgaggagt cacctggaca atcactgctt cggcatccgt cacagttgca
     2881 tatccacaac tttgaggagg gaagcctgga ttcagccaag ttccttgttt cgtttgttca
     2941 atgctttcct tgcattgttc tacagatgga gtgaaggatc ggatggaatg tgttatatac
     3001 ttcggtccat accagcggaa atcacaagta gtgacccatt tggaagcatg acacatccaa
     3061 ccgtctgctt gaatagcctt gtgactcttg ggcattttga cttgtaaggc tgtgcctatt
     3121 aagtcattat gccaatttaa atctgagctt gacgggcaat aatggtaatt agaaggaaca
     3181 tttttccagt ttcctttttg gttgtgtgga aaaactatgg tgaacttgca attcacccca
     3241 atgaataaaa aggctaagta caaaaggcac ttcatggtgg cggtttttta ggagttcgcg
     3301 cccgatggtg ggacggtatg aataatccgg aatatttata ggttttttta ttacaaaact
     3361 gttacgaaaa cagtaaaata cttatttatt tgcgagatgg ttatcatttt aattatctcc
     3421 atgatctatt aatattccgg agtatacgga ccgcggccgc aaatacctgc aggatccgtt
     3481 ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta ttgactagtt
     3541 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
     3601 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
     3661 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
     3721 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
     3781 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
     3841 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
     3901 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
     3961 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
     4021 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
     4081 gggaggtcta tataagcaga gctctctggc taactagaga acccactgct tactggctta
     4141 tcgaaattaa tacgactcac tatagggaga cccaagctgg ctagcgccac catggcttgc
     4201 cccgtccccc tgcagctccc ccccctggag cggctgacac tggatgacaa gaagcccctg
     4261 gacgtgctca tcagcgccac cggcctgtgg atgagccgga caggcaccct gcacaagatt
     4321 aagcaccacg aggtgagccg gagcaagatc tacattgaga tggcctgcgg ggatcacctc
     4381 gtggtgaaca actctaggag ctgccggaca gccagggcct tcaggcacca caagtacagg
     4441 aagacatgca agcggtgcag ggtgtccgat gaggacatca acaactttct gacccggagc
     4501 acagagtcca agaacagcgt gaaggtgagg gtggtgagcg cccccaaggt gaagaaggcc
     4561 atgcccaaga gcgtgtccag ggcccctaag cctctggaga actccgtgag cgccaaggcc
     4621 agcaccaaca ccagccgctc cgtgcccagc cctgccaagt ccaccccaaa cagcagcgtg
     4681 cccgcctccg cccccgcccc ctccctgacc cggagccagc tcgatcgggt ggaggccctg
     4741 ctgagccctg aggataagat ttccctgaac atggccaagc ccttcaggga gctggagcct
     4801 gagctggtca caaggcggaa gaacgatttc cagaggctgt acacaaacga ccgggaggac
     4861 tacctgggga agctggagcg ggacattacc aagttcttcg tcgatagggg cttcctggag
     4921 atcaagagcc ccatcctgat ccctgccgag tacgtcgagc ggatgggcat caacaacgac
     4981 accgagctga gcaagcagat ctttcgggtg gacaagaacc tctgcctgag gcccatgctg
     5041 gccccaactc tgtataacta cctccggaag ctggatcgga ttctgcccgg ccccatcaag
     5101 atctttgagg tcggcccctg ctacaggaag gagagcgatg ggaaggagca cctggaggag
     5161 tttaccatgg tgagctttgg ccagatgggg tccgggtgca caagggagaa cctggaggcc
     5221 ctgatcaagg agttcctgga ctacctggag attgacttcg agatcgtggg cgactcctgt
     5281 atggtgtacg gggacaccct ggatatcatg cacggcgatc tggagctcag cagcgccgcc
     5341 gtggggcccg tgtccctgga tagggagtgg ggcattgata agcccacaat cggggccggg
     5401 ttcggcctgg agaggctgct gaaggtcatg cacggcttta agaacattaa gcgggcctcg
     5461 cggagcgagt cctactacaa cggcattagc accaacctgt aagaattcaa cgcgttaagt
     5521 cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     5581 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     5641 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     5701 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     5761 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     5821 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     5881 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct
     5941 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     6001 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     6061 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcgtcgact
     6121 ttaactcgag tctagagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag
     6181 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
     6241 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
     6301 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
     6361 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagatctcg
     6421 ggcaggaaga gggcctattt cccatgattc cttcatattt gcatatacga tacaaggctg
     6481 ttagagagat aattagaatt aatttgactg taaacacaaa gatattagta caaaatacgt
     6541 gacgtagaaa gtaataattt cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg
     6601 actatcatat gcttaccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
     6661 ggaaaggacg aaacaccgga aacctgatca tgtagatcga acggactcta aatccgttca
     6721 gccgggttag attcccgggg tttccgtttt ttaatatttg catgtcgcta tgtgttctgg
     6781 gaaatcacca taaacgtgaa atccctatca gtgatagaga cttataagtt ccctatcagt
     6841 gatagagaca ccggggggtg gatcgaatag atcacacgga ctctaaatcc gtgcaggcgg
     6901 gtgaaactcc cgcacccccc gttttttcag ctggggctct agggggtatc cccacgcgcc
     6961 ctgtagcggc gcattaagcg caagccctgc catagccact acgggtacgt agccaaccac
     7021 tagaactata gctagagtcc tgggcgaaca aacgatgctc gccttccaga aaaccgagga
     7081 tgcgaaccac ttcatccggg gtcagcacca ccggcaagcg ccgcgacggc cgaggtctac
     7141 cgatctcctg aagcaagggc agatccgtgc acagcacctt gccgtagaag aacagcaagg
     7201 ccgccaatgc ctgacgatgc gtggagaccg aaaccttgcg ctcgttcgcc agccaggaca
     7261 gaaatgcctc gacttcgctg ctgcccaagg ttgccgggtg acgcacaccg tggaaacgga
     7321 tgaaggcacg aacccagttg acataagcct gttcggttcg taaactgtaa tgcaagtagc
     7381 gtatgcgctc acgcaactgg tccagaacct tgaccgaacg cagcggtggt aacggcgcag
     7441 tggcggtttt catggcttgt tatgactgtt tttttgtaca gtctatgcct cgggcatcca
     7501 agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc aacgatgtta
     7561 cgcagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt aggtggctca
     7621 agtatgggca tcattcgcac atgtaggctc ggccctgacc aagtcaaatc catgcgggct
     7681 gctcttgatc ttttcggtcg tgagttcgga gacgtagcca cctactccca acatcagccg
     7741 gactccgatt acctcgggaa cttgctccgt agtaagacat tcatcgcgct tgctgccttc
     7801 gaccaagaag cggttgttgg cgctctcgcg gcttacgttc tgcccaagtt tgagcagccg
     7861 cgtagtgaga tctatatcta tgatctcgca gtctccggcg agcaccggag gcagggcatt
     7921 gccaccgcgc tcatcaatct cctcaagcat gaggccaacg cgcttggtgc ttatgtgatc
     7981 tacgtgcaag cagattacgg tgacgatccc gcagtggctc tctatacaaa gttgggcata
     8041 cgggaagaag tgatgcactt tgatatcgac ccaagtaccg ccacctaaca attcgttcaa
     8101 gccgagatcg gcttcccggc cgcggagttg ttcggtaaat tgtcacaacg ccgcgaatat
     8161 agtctttacc atgcccttgg ccacgcccct ctttaatacg acgggcaatt tgcacttcag
     8221 aaaatgaaga gtttgcttta gccataacaa aagtccagta tgctttttca cagcataact
     8281 ggactgattt cagtttacaa ctattctgtc tagtttaaga ctttattgtc atagtttaga
     8341 tctattttgt tcagtttaag actttattgt ccgcccacac ccgcttacgc agggcatcca
     8401 tttattactc aaccgtaacc gattttgcca ggttacgcgg ctggtctatg cggtgtgaaa
     8461 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca
     8521 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     8581 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     8641 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     8701 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     8761 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     8821 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     8881 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     8941 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     9001 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     9061 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     9121 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     9181 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     9241 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     9301 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     9361 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     9421 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     9481 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     9541 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     9601 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     9661 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     9721 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     9781 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     9841 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     9901 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     9961 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
    10021 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
    10081 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
    10141 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
    10201 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
    10261 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
    10321 at
//
