LOCUS       sequence-345742-       14362 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Binary vector for Agrobacterium-mediated plant transformation - 
            Promoter:GUS reporter for expression analysis of GOXL genes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14362)
  AUTHORS   Sola K, Dean GH, Li Y, Lohmann J, Movahedan M, Gilchrist EJ, Adams 
            KL, Haughn GW
  TITLE     Expression Patterns and Functional Characterisation of Arabidopsis 
            GALACTOSE OXIDASE-LIKE (GOXL) Genes Suggest Specialised Roles for 
            Galactose Oxidases in Plants.
  JOURNAL   Plant Cell Physiol. 2021 May 27. pii: 6286013. doi: 
            10.1093/pcp/pcab073.
  PUBMED    34042158
REFERENCE   2  (bases 1 to 14362)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell 
            Physiol. 2021 May 27. pii: 6286013. doi: 10.1093/pcp/pcab073."
COMMENT     Sequence Label: pBI101.1/ProGOXL1:GUS
FEATURES             Location/Qualifiers
     source          1..14362
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        147..326
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        179..362
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             383..1177
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(437..456)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1047..1066
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      1567..1819
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    2353..2374
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2389..2394,2395..2412,2413..2419)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2427..2443
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2432..2454
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2451..2467
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2451..2467
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2994..4805
                     /codon_start=1
                     /gene="uidA (or gusA) from E. coli"
                     /product="beta-glucuronidase"
                     /label=GUS
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
                     PKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     terminator      4876..5128
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(5137..5154)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5137..5153)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5146..5168)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(5293..5312)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5503..5524
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    5771..5795
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(7391..8539)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(8838..9632)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  9824..10591
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      11214..11925
                     /label=oriV
                     /note="incP origin of replication"
     CDS             complement(12052..12423)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            12456..12565
                     /note="incP origin of transfer"
     primer_bind     12788..12807
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13041..13058
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(13246..13896)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    join(14361..14362,1..23)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ttacccgcca atatatcctg tcaaacactg atagtttaaa ctgaaggcgg gaaacgacaa
       61 tctgatcatg agcggagaat taagggagtc acgttatgac ccccgccgat gacgcgggac
      121 aagccgtttt acgtttggaa ctgacagaac cgcaacgttg aaggagccac tcagccgcgg
      181 gtttctggag tttaatgagc taagcacata cgtcagaaac cattattgcg cgttcaaaag
      241 tcgcctaagg tcactatcag ctagcaaata tttcttgtca aaaatgctcc actgacgttc
      301 cataaattcc cctcggtatc caattagagt ctcatattca ctctcaatcc aaataatctg
      361 caccggatct ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc
      421 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga
      481 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct
      541 gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac
      601 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct
      661 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt
      721 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt
      781 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt
      841 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag
      901 gctcaaggcg cgcatgcccg acggcgatga tctcgtcgtg acccatggcg atgcctgctt
      961 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg
     1021 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg
     1081 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg
     1141 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg
     1201 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat
     1261 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
     1321 gatctcatgc tggagttctt cgcccacggg atctctgcgg aacaggcggt cgaaggtgcc
     1381 gatatcatta cgacagcaac ggccgacaag cacaacgcca cgatcctgag cgacaatatg
     1441 atcgggcccg gcgtccacat caacggcgtc ggcggcgact gcccaggcaa gaccgagatg
     1501 caccgcgata tcttgctgcg ttcggatatt ttcgtggagt tcccgccaca gacccggatg
     1561 atccccgatc gttcaaacat ttggcaataa agtttcttaa gattgaatcc tgttgccggt
     1621 cttgcgatga ttatcatata atttctgttg aattacgtta agcatgtaat aattaacatg
     1681 taatgcatga cgttatttat gagatgggtt tttatgatta gagtcccgca attatacatt
     1741 taatacgcga tagaaaacaa aatatagcgc gcaaactagg ataaattatc gcgcgcggtg
     1801 tcatctatgt tactagatcg ggcctcctgt caatgctggc ggcggctctg gtggtggttc
     1861 tggtggcggc tctgagggtg gtggctctga gggtggcggc tctgagggag gcggttccgg
     1921 tggtggctct ggttccggtg attttgatta tgaaaagatg gcaaacgcta ataagggggc
     1981 tatgaccgaa aatgccgatg aaaacgcgct acagtctgac gctaaaggca aacttgattc
     2041 tgtcgctact gattacggtg ctgctatcga tggtttcatt ggtgacgttt ccggccttgc
     2101 taatggtaat ggtgctactg gtgattttgc tggctctaat tcccaaatgg ctcaagtcgg
     2161 tgacggtgat aattcacctt taatgaataa tttccgtcaa tatttacctt ccctccctca
     2221 atcggttgaa tgtcgccctt ttgtctttgg cccaatacgc aaaccgcctc tccccgcgcg
     2281 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     2341 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
     2401 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
     2461 ctatgaccat gattacgcca agcttgcatg cctgcaggtc gacttattgt ctctattaga
     2521 aaactcttgg gtttaaatag tcacaatgat acaggcatgt aagtgccagg caaaatttca
     2581 aaagtgtgac taaagcatac atcaaaatct taggtagaag ctttccactt tatataatct
     2641 caaaatctta ggtagaagca aacataatct caaaatctta ggtagaagct ttccacttta
     2701 tataaatgtt tttttttgca aaatcattat gttcaaatac tatatttttg ggtttacgca
     2761 acaaatttaa cgctgtaagc caacgtgaga aaaagaaaaa atatagccct atcatttgtt
     2821 tttcttaaag aaccaccata caaaatgaaa ggcaaacgta tagaggaaat aatttgccaa
     2881 ccactcattc caataaaaga aaggtcaaaa ataaacacaa gccaaactat tcccaagtat
     2941 ataaagcaat gttcactaga caaaccatcg gatccccggg tggtcagtcc cttatgttac
     3001 gtcctgtaga aaccccaacc cgtgaaatca aaaaactcga cggcctgtgg gcattcagtc
     3061 tggatcgcga aaactgtgga attgatcagc gttggtggga aagcgcgtta caagaaagcc
     3121 gggcaattgc tgtgccaggc agttttaacg atcagttcgc cgatgcagat attcgtaatt
     3181 atgcgggcaa cgtctggtat cagcgcgaag tctttatacc gaaaggttgg gcaggccagc
     3241 gtatcgtgct gcgtttcgat gcggtcactc attacggcaa agtgtgggtc aataatcagg
     3301 aagtgatgga gcatcagggc ggctatacgc catttgaagc cgatgtcacg ccgtatgtta
     3361 ttgccgggaa aagtgtacgt atcaccgttt gtgtgaacaa cgaactgaac tggcagacta
     3421 tcccgccggg aatggtgatt accgacgaaa acggcaagaa aaagcagtct tacttccatg
     3481 atttctttaa ctatgccgga atccatcgca gcgtaatgct ctacaccacg ccgaacacct
     3541 gggtggacga tatcaccgtg gtgacgcatg tcgcgcaaga ctgtaaccac gcgtctgttg
     3601 actggcaggt ggtggccaat ggtgatgtca gcgttgaact gcgtgatgcg gatcaacagg
     3661 tggttgcaac tggacaaggc actagcggga ctttgcaagt ggtgaatccg cacctctggc
     3721 aaccgggtga aggttatctc tatgaactgt gcgtcacagc caaaagccag acagagtgtg
     3781 atatctaccc gcttcgcgtc ggcatccggt cagtggcagt gaagggcgaa cagttcctga
     3841 ttaaccacaa accgttctac tttactggct ttggtcgtca tgaagatgcg gacttgcgtg
     3901 gcaaaggatt cgataacgtg ctgatggtgc acgaccacgc attaatggac tggattgggg
     3961 ccaactccta ccgtacctcg cattaccctt acgctgaaga gatgctcgac tgggcagatg
     4021 aacatggcat cgtggtgatt gatgaaactg ctgctgtcgg ctttaacctc tctttaggca
     4081 ttggtttcga agcgggcaac aagccgaaag aactgtacag cgaagaggca gtcaacgggg
     4141 aaactcagca agcgcactta caggcgatta aagagctgat agcgcgtgac aaaaaccacc
     4201 caagcgtggt gatgtggagt attgccaacg aaccggatac ccgtccgcaa ggtgcacggg
     4261 aatatttcgc gccactggcg gaagcaacgc gtaaactcga cccgacgcgt ccgatcacct
     4321 gcgtcaatgt aatgttctgc gacgctcaca ccgataccat cagcgatctc tttgatgtgc
     4381 tgtgcctgaa ccgttattac ggatggtatg tccaaagcgg cgatttggaa acggcagaga
     4441 aggtactgga aaaagaactt ctggcctggc aggagaaact gcatcagccg attatcatca
     4501 ccgaatacgg cgtggatacg ttagccgggc tgcactcaat gtacaccgac atgtggagtg
     4561 aagagtatca gtgtgcatgg ctggatatgt atcaccgcgt ctttgatcgc gtcagcgccg
     4621 tcgtcggtga acaggtatgg aatttcgccg attttgcgac ctcgcaaggc atattgcgcg
     4681 ttggcggtaa caagaaaggg atcttcactc gcgaccgcaa accgaagtcg gcggcttttc
     4741 tgctgcaaaa acgctggact ggcatgaact tcggtgaaaa accgcagcag ggaggcaaac
     4801 aatgaatcaa caactctcct ggcgcaccat cgtcggctac agcctcggga attgctaccg
     4861 agctcgaatt tccccgatcg ttcaaacatt tggcaataaa gtttcttaag attgaatcct
     4921 gttgccggtc ttgcgatgat tatcatataa tttctgttga attacgttaa gcatgtaata
     4981 attaacatgt aatgcatgac gttatttatg agatgggttt ttatgattag agtcccgcaa
     5041 ttatacattt aatacgcgat agaaaacaaa atatagcgcg caaactagga taaattatcg
     5101 cgcgcggtgt catctatgtt actagatcgg gaattcactg gccgtcgttt tacaacgtcg
     5161 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
     5221 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
     5281 gaatggcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     5341 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     5401 tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga
     5461 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     5521 ctggaacaac actcaaccct atctcgggct attcttttga tttataaggg attttgccga
     5581 tttcggaacc accatcaaac aggattttcg cctgctgggg caaaccagcg tggaccgctt
     5641 gctgcaactc tctcagggcc aggcggtgaa gggcaatcag ctgttgcccg tctcactggt
     5701 gaaaagaaaa accaccccag tacattaaaa acgtccgcaa tgtgttatta agttgtctaa
     5761 gcgtcaattt gtttacacca caatatatcc tgccaccagc cagccaacag ctccccgacc
     5821 ggcagctcgg cacaaaatca ccactcgata caggcagccc atcagtccgg gacggcgtca
     5881 gcgggagagc cgttgtaagg cggcagactt tgctcatgtt accgatgcta ttcggaagaa
     5941 cggcaactaa gctgccgggt ttgaaacacg gatgatctcg cggagggtag catgttgatt
     6001 gtaacgatga cagagcgttg ctgcctgtga tcaaatatca tctccctcgc agagatccga
     6061 attatcagcc ttcttattca tttctcgctt aaccgtgaca ggctgtcgat cttgagaact
     6121 atgccgacat aataggaaat cgctggataa agccgctgag gaagctgagt ggcgctattt
     6181 ctttagaagt gaacgttgac gatatcaact cccctatcca ttgctcaccg aatggtacag
     6241 gtcggggacc cgaagttccg actgtcggcc tgatgcatcc ccggctgatc gaccccagat
     6301 ctggggctga gaaagcccag taaggaaaca actgtaggtt cgagtcgcga gatcccccgg
     6361 aaccaaagga agtaggttaa acccgctccg atcaggccga gccacgccag gccgagaaca
     6421 ttggttcctg taggcatcgg gattggcgga tcaaacacta aagctactgg aacgagcaga
     6481 agtcctccgg ccgccagttg ccaggcggta aaggtgagca gaggcacggg aggttgccac
     6541 ttgcgggtca gcacggttcc gaacgccatg gaaaccgccc ccgccaggcc cgctgcgacg
     6601 ccgacaggat ctagcgctgc gtttggtgtc aacaccaaca gcgccacgcc cgcagttccg
     6661 caaatagccc ccaggaccgc catcaatcgt atcgggctac ctagcagagc ggcagagatg
     6721 aacacgacca tcagcggctg cacagcgcct accgtcgccg cgaccccgcc cggcaggcgg
     6781 tagaccgaaa taaacaacaa gctccagaat agcgaaatat taagtgcgcc gaggatgaag
     6841 atgcgcatcc accagattcc cgttggaatc tgtcggacga tcatcacgag caataaaccc
     6901 gccggcaacg cccgcagcag cataccggcg acccctcggc ctcgctgttc gggctccacg
     6961 aaaacgccgg acagatgcgc cttgtgagcg tccttggggc cgtcctcctg tttgaagacc
     7021 gacagcccaa tgatctcgcc gtcgatgtag gcgccgaatg ccacggcatc tcgcaaccgt
     7081 tcagcgaacg cctccatggg ctttttctcc tcgtgctcgt aaacggaccc gaacatctct
     7141 ggagctttct tcagggccga caatcggatc tcgcggaaat cctgcacgtc ggccgctcca
     7201 agccgtcgaa tctgagcctt aatcacaatt gtcaatttta atcctctgtt tatcggcagt
     7261 tcgtagagcg cgccgtgcgt cccgagcgat actgagcgaa gcaagtgcgt cgagcagtgc
     7321 ccgcttgttc ctgaaatgcc agtaaagcgc tggctgctga acccccagcc ggaactgacc
     7381 ccacaaggcc ctagcgtttg caatgcacca ggtcatcatt gacccaggcg tgttccacca
     7441 ggccgctgcc tcgcaactct tcgcaggctt cgccgacctg ctcgcgccac ttcttcacgc
     7501 gggtggaatc cgatccgcac atgaggcgga aggtttccag cttgagcggg tacggctccc
     7561 ggtgcgagct gaaatagtcg aacatccgtc gggccgtcgg cgacagcttg cggtacttct
     7621 cccatatgaa tttcgtgtag tggtcgccag caaacagcac gacgatttcc tcgtcgatca
     7681 ggacctggca acgggacgtt ttcttgccac ggtccaggac gcggaagcgg tgcagcagcg
     7741 acaccgattc caggtgccca acgcggtcgg acgtgaagcc catcgccgtc gcctgtaggc
     7801 gcgacaggca ttcctcggcc ttcgtgtaat accggccatt gatcgaccag cccaggtcct
     7861 ggcaaagctc gtagaacgtg aaggtgatcg gctcgccgat aggggtgcgc ttcgcgtact
     7921 ccaacacctg ctgccacacc agttcgtcat cgtcggcccg cagctcgacg ccggtgtagg
     7981 tgatcttcac gtccttgttg acgtggaaaa tgaccttgtt ttgcagcgcc tcgcgcggga
     8041 ttttcttgtt gcgcgtggtg aacagggcag agcgggccgt gtcgtttggc atcgctcgca
     8101 tcgtgtccgg ccacggcgca atatcgaaca aggaaagctg catttccttg atctgctgct
     8161 tcgtgtgttt cagcaacgcg gcctgcttgg cctcgctgac ctgttttgcc aggtcctcgc
     8221 cggcggtttt tcgcttcttg gtcgtcatag ttcctcgcgt gtcgatggtc atcgacttcg
     8281 ccaaacctgc cgcctcctgt tcgagacgac gcgaacgctc cacggcggcc gatggcgcgg
     8341 gcagggcagg gggagccagt tgcacgctgt cgcgctcgat cttggccgta gcttgctgga
     8401 ccatcgagcc gacggactgg aaggtttcgc ggggcgcacg catgacggtg cggcttgcga
     8461 tggtttcggc atcctcggcg gaaaaccccg cgtcgatcag ttcttgcctg tatgccttcc
     8521 ggtcaaacgt ccgattcatt caccctcctt gcgggattgc cccgactcac gccggggcaa
     8581 tgtgccctta ttcctgattt gacccgcctg gtgccttggt gtccagataa tccaccttat
     8641 cggcaatgaa gtcggtcccg tagaccgtct ggccgtcctt ctcgtacttg gtattccgaa
     8701 tcttgccctg cacgaatacc agcgacccct tgcccaaata cttgccgtgg gcctcggcct
     8761 gagagccaaa acacttgatg cggaagaagt cggtgcgctc ctgcttgtcg ccggcatcgt
     8821 tgcgccacat ctaggtacta aaacaattca tccagtaaaa tataatattt tattttctcc
     8881 caatcaggct tgatccccag taagtcaaaa aatagctcga catactgttc ttccccgata
     8941 tcctccctga tcgaccggac gcagaaggca atgtcatacc acttgtccgc cctgccgctt
     9001 ctcccaagat caataaagcc acttactttg ccatctttca caaagatgtt gctgtctccc
     9061 aggtcgccgt gggaaaagac aagttcctct tcgggctttt ccgtctttaa aaaatcatac
     9121 agctcgcgcg gatctttaaa tggagtgtct tcttcccagt tttcgcaatc cacatcggcc
     9181 agatcgttat tcagtaagta atccaattcg gctaagcggc tgtctaagct attcgtatag
     9241 ggacaatccg atatgtcgat ggagtgaaag agcctgatgc actccgcata cagctcgata
     9301 atcttttcag ggctttgttc atcttcatac tcttccgagc aaaggacgcc atcggcctca
     9361 ctcatgagca gattgctcca gccatcatgc cgttcaaagt gcaggacctt tggaacaggc
     9421 agctttcctt ccagccatag catcatgtcc ttttcccgtt ccacatcata ggtggtccct
     9481 ttataccggc tgtccgtcat ttttaaatat aggttttcat tttctcccac cagcttatat
     9541 accttagcag gagacattcc ttccgtatct tttacgcagc ggtatttttc gatcagtttt
     9601 ttcaattccg gtgatattct cattttagcc atttattatt tccttcctct tttctacagt
     9661 atttaaagat accccaagaa gctaattata acaagacgaa ctccaattca ctgttccttg
     9721 cattctaaaa ccttaaatac cagaaaacag ctttttcaaa gttgttttca aagttggcgt
     9781 ataacatagt atcgacggag ccgattttga aaccacaatt atgggtgatg ctgccaactt
     9841 actgatttag tgtatgatgg tgtttttgag gtgctccagt ggcttctgtt tctatcagct
     9901 gtccctcctg ttcagctact gacggggtgg tgcgtaacgg caaaagcacc gccggacatc
     9961 agcgctatct ctgctctcac tgccgtaaaa catggcaact gcagttcact tacaccgctt
    10021 ctcaacccgg tacgcaccag aaaatcattg atatggccat gaatggcgtt ggatgccggg
    10081 caacagcccg cattatgggc gttggcctca acacgatttt acgtcactta aaaaactcag
    10141 gccgcagtcg gtaacctcgc gcatacagcc gggcagtgac gtcatcgtct gcgcggaaat
    10201 ggacgaacag tggggctatg tcggggctaa atcgcgccag cgctggctgt tttacgcgta
    10261 tgacagtctc cggaagacgg ttgttgcgca cgtattcggt gaacgcacta tggcgacgct
    10321 ggggcgtctt atgagcctgc tgtcaccctt tgacgtggtg atatggatga cggatggctg
    10381 gccgctgtat gaatcccgcc tgaagggaaa gctgcacgta atcagcaagc gatatacgca
    10441 gcgaattgag cggcataacc tgaatctgag gcagcacctg gcacggctgg gacggaagtc
    10501 gctgtcgttc tcaaaatcgg tggagctgca tgacaaagtc atcgggcatt atctgaacat
    10561 aaaacactat caataagttg gagtcattac ccaattatga tagaatttac aagctataag
    10621 gttattgtcc tgggtttcaa gcattagtcc atgcaagttt ttatgctttg cccattctat
    10681 agatatattg ataagcgcgc tgcctatgcc ttgccccctg aaatccttac atacggcgat
    10741 atcttctata taaaagatat attatcttat cagtattgtc aatatattca aggcaatctg
    10801 cctcctcatc ctcttcatcc tcttcgtctt ggtagctttt taaatatggc gcttcataga
    10861 gtaattctgt aaaggtccaa ttctcgtttt catacctcgg tataatctta cctatcacct
    10921 caaatggttc gctgggttta tcgcaccccc gaacacgagc acggcacccg cgaccactat
    10981 gccaagaatg cccaaggtaa aaattgccgg ccccgccatg aagtccgtga atgccccgac
    11041 ggccgaagtg aagggcaggc cgccacccag gccgccgccc tcactgcccg gcacctggtc
    11101 gctgaatgtc gatgccagca cctgcggcac gtcaatgctt ccgggcgtcg cgctcgggct
    11161 gatcgcccat cccgttactg ccccgatccc ggcaatggca aggactgcca gcgctgccat
    11221 ttttggggtg aggccgttcg cggccgaggg gcgcagcccc tggggggatg ggaggcccgc
    11281 gttagcgggc cgggagggtt cgagaagggg gggcaccccc cttcggcgtg cgcggtcacg
    11341 cgcacagggc gcagccctgg ttaaaaacaa ggtttataaa tattggttta aaagcaggtt
    11401 aaaagacagg ttagcggtgg ccgaaaaacg ggcggaaacc cttgcaaatg ctggattttc
    11461 tgcctgtgga cagcccctca aatgtcaata ggtgcgcccc tcatctgtca gcactctgcc
    11521 cctcaagtgt caaggatcgc gcccctcatc tgtcagtagt cgcgcccctc aagtgtcaat
    11581 accgcagggc acttatcccc aggcttgtcc acatcatctg tgggaaactc gcgtaaaatc
    11641 aggcgttttc gccgatttgc gaggctggcc agctccacgt cgccggccga aatcgagcct
    11701 gcccctcatc tgtcaacgcc gcgccgggtg agtcggcccc tcaagtgtca acgtccgccc
    11761 ctcatctgtc agtgagggcc aagttttccg cgaggtatcc acaacgccgg cggccgcggt
    11821 gtctcgcaca cggcttcgac ggcgtttctg gcgcgtttgc agggccatag acggccgcca
    11881 gcccagcggc gagggcaacc agcccggtga gcgtcggaaa ggcgctcggt cttgccttgc
    11941 tcgtcggtga tgtacttcac cagctccgcg aagtcgctct tcttgatgga gcgcatgggg
    12001 acgtgcttgg caatcacgcg caccccccgg ccgttttagc ggctaaaaaa gtcatggctc
    12061 tgccctcggg cggaccacgc ccatcatgac cttgccaagc tcgtcctgct tctcttcgat
    12121 cttcgccagc agggcgagga tcgtggcatc accgaaccgc gccgtgcgcg ggtcgtcggt
    12181 gagccagagt ttcagcaggc cgcccaggcg gcccaggtcg ccattgatgc gggccagctc
    12241 gcggacgtgc tcatagtcca cgacgcccgt gattttgtag ccctggccga cggccagcag
    12301 gtaggccgac aggctcatgc cggccgccgc cgccttttcc tcaatcgctc ttcgttcgtc
    12361 tggaaggcag tacaccttga taggtgggct gcccttcctg gttggcttgg tttcatcagc
    12421 catccgcttg ccctcatctg ttacgccggc ggtagccggc cagcctcgca gagcaggatt
    12481 cccgttgagc accgccaggt gcgaataagg gacagtgaag aaggaacacc cgctcgcggg
    12541 tgggcctact tcacctatcc tgcccggctg acgccgttgg atacaccaag gaaagtctac
    12601 acgaaccctt tggcaaaatc ctgtatatcg tgcgaaaaag gatggatata ccgaaaaaat
    12661 cgctataatg accccgaagc agggttatgc agcggaaaag cgccacgctt cccgaaggga
    12721 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
    12781 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
    12841 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
    12901 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
    12961 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
    13021 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgccagaagg
    13081 ccgccagaga ggccgagcgc ggccgtgagg cttggacgct agggcagggc atgaaaaagc
    13141 ccgtagcggg ctgctacggg cgtctgacgc ggtggaaagg gggaggggat gttgtctaca
    13201 tggctctgct gtagtgagtg ggttgcgctc cggcagcggt cctgatcaat cgtcaccctt
    13261 tctcggtcct tcaacgttcc tgacaacgag cctccttttc gccaatccat cgacaatcac
    13321 cgcgagtccc tgctcgaacg ctgcgtccgg accggcttcg tcgaaggcgt ctatcgcggc
    13381 ccgcaacagc ggcgagagcg gagcctgttc aacggtgccg ccgcgctcgc cggcatcgct
    13441 gtcgccggcc tgctcctcaa gcacggcccc aacagtgaag tagctgattg tcatcagcgc
    13501 attgacggcg tccccggccg aaaaacccgc ctcgcagagg aagcgaagct gcgcgtcggc
    13561 cgtttccatc tgcggtgcgc ccggtcgcgt gccggcatgg atgcgcgcgc catcgcggta
    13621 ggcgagcagc gcctgcctga agctgcgggc attcccgatc agaaatgagc gccagtcgtc
    13681 gtcggctctc ggcaccgaat gcgtatgatt ctccgccagc atggcttcgg ccagtgcgtc
    13741 gagcagcgcc cgcttgttcc tgaagtgcca gtaaagcgcc ggctgctgaa cccccaaccg
    13801 ttccgccagt ttgcgtgtcg tcagaccgtc tacgccgacc tcgttcaaca ggtccagggc
    13861 ggcacggatc actgtattcg gctgcaactt tgtcatgctt gacactttat cactgataaa
    13921 cataatatgt ccaccaactt atcagtgata aagaatccgc gcgttcaatc ggaccagcgg
    13981 aggctggtcc ggaggccaga cgtgaaaccc aacatacccc tgatcgtaat tctgagcact
    14041 gtcgcgctcg acgctgtcgg catcggcctg attatgccgg tgctgccggg cctcctgcgc
    14101 gatctggttc actcgaacga cgtcaccgcc cactatggca ttctgctggc gctgtatgcg
    14161 ttggtgcaat ttgcctgcgc acctgtgctg ggcgcgctgt cggatcgttt cgggcggcgg
    14221 ccaatcttgc tcgtctcgct ggccggcgcc agatctgggg aaccctgtgg ttggcatgca
    14281 catacaaatg gacgaacgga taaacctttt cacgcccttt taaatatccg attattctaa
    14341 taaacgctct tttctcttag gt
//
