LOCUS       sequence-345745-       15513 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus and mammalian expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15513)
  TITLE     SGC Toronto 2019-Q1 to 2020-Q2
REFERENCE   2  (bases 1 to 15513)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: HTT delta-exon1
FEATURES             Location/Qualifiers
     source          1..15513
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  218..383
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     polyA_signal    412..546
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(436..455)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     490..509
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             771..782
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     1180..1200
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1290..1313)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(1314..1343)
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             complement(1344..1364)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     regulatory      2041..2050
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(10597..10706)
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     promoter        complement(10753..10956)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(10782..10802)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        10957..11336
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(11397..11416)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    11441..11489
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        join(11997..12002,12003..12019,12020..12025)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             12214..12747
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     mobile_element  12814..13038
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     primer_bind     complement(13172..13189)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13343..13931)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13423..13442)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(14102..14893,14894..14962))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14725..14744
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14963..15067)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(join(15093..15513,1..34))
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(15230..15251)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     15441..15460
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt cgccattcag gctgcaaata
       61 agcgttgata ttcagtcaat tacaaacatt aataacgaag agatgacaga aaaattttca
      121 ttctgtgaca gagaaaaagt agccgaagat gacggtttgt cacatggagt tggcaggatg
      181 tttgattaaa aacataacag gaagaaaaat gccccgctgt gggcggacaa aatagttggg
      241 aactgggagg ggtggaaatg gagtttttaa ggattattta gggaagagtg acaaaataga
      301 tgggaactgg gtgtagcgtc gtaagctaat acgaaaatta aaaatgacaa aatagtttgg
      361 aactagattt cacttatctg gttcggatct cctaggctca agcagtgatc agatccagac
      421 atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc
      481 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa
      541 caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag
      601 gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga tcctctagta
      661 cttctcgaca agcttgtcga gactgcaggc tctagagatc ctctagtact tctcgacaag
      721 cttcgctcgc cgtgaggagg ggggcgcccg cgggaaggaa ggtccgctgg attgagggcc
      781 gaagggacgt agcagaagga cgtcccgcgc agaatccagg tggcaacaca ggcgagcagc
      841 caaggaaagg acgatgattt ccccgacaac accacggaat tgtcagtgcc caacagccga
      901 gcccctgtcc agcagcgggc aaggcaggcg gcgatgagtt ccgccgtggc aatagggagg
      961 gggaaagcga aagtcccgga aaggagctga caggtggtgg caatgcccca accagtgggg
     1021 gttgcgtcag caaacacagt gcacaccacg ccacgttgcc tgacaacggg ccacaactcc
     1081 tcataaagag acagcaacca ggatttatac aaggaggaga aaatgaaagc catacgggaa
     1141 gcaatagcat gatacaaagg cattaaagca gcgtatccac atagcgtaaa aggagcaaca
     1201 tagttaagaa taccagtcaa tctttcacaa attttgtaat ccagaggttg attatcgata
     1261 agcttgatat caagttggcg gccgcctcac ttatcgtcgt catccttgta atcatggtgg
     1321 tgatggtgat ggtgatgatg gtgcgattgg aagtagaggt tctctgcgca ggtggtgacc
     1381 ttgtggacat ttcgtaaaca agtcagcagc cggtgatatg ggcttcctgg ggctgcaacc
     1441 acctcaagca cagactggaa ggccctgcgg tcgagctcct cctctatctg gtgtctgtag
     1501 aagtctgtgg cgaccaggca gaaaaggttc acgtccacct gctccagctt gcccatcctg
     1561 ctgatgacat gtgggaggat cgccgcgacc cacgggctgg tggacgcgct gacaaagaag
     1621 caggagaggc tccacgtggc catggcgacc ggggccctct gcgtgaagtt ggagagggac
     1681 agcatgaccc agtcccggac catggacgac tgcccggtgc tgtgcagagt ctgaaacacc
     1741 ttatacacca cggtggccat gaactggggg tatggctgct ggttggacag aaactctccg
     1801 atgactttgt tcatgatgtc ctggggtggg aagaagtcgt ctagaaactg gggcaggatc
     1861 ctggccacca ctctggcttc acaaggaaag cctttcctga tcctatcaaa aagaacagat
     1921 acccgctcca tagcaacaat cactgactcg ctgtcggggg ctgcaggatt agggtctgaa
     1981 gttctacccg gactgacttt ctcctttcct gtgtacatgc aggtgagcat caggcccaga
     2041 gccgccatgg cccggtgcgg gctgtgcacg ttcactctgt ccacactcag cttgaccagc
     2101 gattctgcat ccaggcggga gagctgctca gagagcagga ggcgctccag gcctctgagg
     2161 gcacagtggt aaatgatgga gggggtggac tcctcacttc cagacagcat caccccacac
     2221 atctgtatta ttgatgctga aaattccggc cctacgtcca gaggatagtt ctcaatgagg
     2281 taaaacgcag tggcacacat gaccagtacg tgctgctggc tgtgaatgtt cacgcagtgg
     2341 gcgatccctt tcaggttgga gaggagatag tcgctgatga ccgggatgag ctgcttggca
     2401 gtgtcgtcca gcaggtcgca ctccagcaca tagaggatgc cgtgcagggc tccaaccctg
     2461 ctgggcaggt ggctgctcct gagcgtgctc tccagcaggc ggctgacagg ctccgccacg
     2521 gccttgtcca tcccaaggac ggcagctgcc ttgcaggtgg caggcaccag gtactgagcg
     2581 aggatctcgt cttctgaagg gtgcaccctt cgcagttctg tcagcgtcac atacatcagc
     2641 tcaaactggt tgcgctcggt gaacaagtct gagaccacta gaagggatct gaccacctca
     2701 ctgatcagga tggccggggt cctcctggct gagctggacg gcaggatcca gcggctgtac
     2761 aactcaagca aaaactgcga acaggagtgg atgtcaactc cagcccggtg tttcctggag
     2821 ttgactggag acgtgggtgg tgacgaaggt gcaggggcgt cggcctcctc ctcctcttcc
     2881 tcgtcccatt cctcctccct caggggtgtg atgctgttcc ctagccacac ggagtgtatg
     2941 gacacctggc cgagtttgta gctcatgctc cccagctccc gctcggggtt gatctgcagc
     3001 agcagcttct cgtggctgat gagggcacct gtagtagccg gagacagaga agggacagga
     3061 tcccatgcct gatataaatg atgggtggca atattctctc tctttgaaac cattgcttga
     3121 atctcttgct ccacaatccc tctgataatg ctcagcttcc tcccaaacct ggtgtcgaga
     3181 gctttcagag gcttgttccg gggctgctgc tccaagcagc ttacagctgg gttgccggcc
     3241 acaggcacag tcattgcact gagcaccagt gaggtgatgg cctgcacggc caggacgttg
     3301 atctgggtcc tctctgtgtc ttcttctggt gggctctcct cctgctccat cacgaggggc
     3361 tgcgtcacca ggacaccaag gagggtggcc caagtttctt caaactgagt acgactggtc
     3421 cagcctagtg tgttgatgcg gtagatgaac tccttaaaga cttccttttc ctggaggaac
     3481 tccacgggga tctcagggaa tgctgtgcca aaatcccctc ccggtttggg tgaccatcca
     3541 agcttccaca ccagtggggg cacacgtgtg tagctgttga caaggggcag gcgggccagg
     3601 ctgatgatga tgttcctgag caatggcgtg agaaacgccg gcacgccgct attcctttta
     3661 tgacccaagg ccaacaccga ctgcagagac tccaccattt ctgccaccat ctcacaggct
     3721 gcagtgatat acttaggatt ctgtgtgttt ggatctactt cctcctcctc ctcgctgatg
     3781 gcttttgggg tatttgtcct tctttctgga ctaagaagct gctctccagg ctgcactgca
     3841 acggcctcca ggatgaagtg cacacagtgg atgagggagc aggcgtgggt cacaaactct
     3901 gtggaggaga ccacgctcca gaggccaggc agctgcaggg ccaggcagca gcagtccagc
     3961 cctgcctgga gatccagact cagcgggatc tgctcatgga tcaaatgcca ggacagggcc
     4021 tcaagggttg ccaccacgaa tttcacaatg tccttctctt tctcaggagg aaggtgcaaa
     4081 tgactgggca gtttggagac caccaccagg tactgtgcca gggcccgggc cagagtgggc
     4141 agggactgat acagtgcagc atccccaaac agatcattca acttgctcca gtaggccgcc
     4201 ggttctgcag gcagctcggg ctggaagaca tgatggacag cagggagctg ctgcacggtg
     4261 ccgctcacac gggccagagt cacctcacgg gctgcttcaa aaagggcact cttctggcca
     4321 ccagaaattt cactcatccc taggcttaag catggagcta gcaggcttag gttgaactcc
     4381 gagttcatca tgaaggcatt catatcttca gcaggaatcc gattcaccag ctctgcacct
     4441 tccagcagtg cagaatctga cctggtccaa cactgggatt tgacaagatg aacgtaccag
     4501 tctttgtccg gactcactgt ttccagtgac acgtgcccat ccccgtccag cgggtgggaa
     4561 gagactggag gagagggact aagtgagtct tgcatggtgg agagacgaaa cctgtccagc
     4621 agggaataga gcctttggtg tctctgagcg agcccgctgc tctgaaggta ttcctggatt
     4681 ctgttgagtt cttccattgg caactgggcc atgctgctct gtaaatttgc agccagaagc
     4741 atttctaccc ggcgacaagc aaggatgtcg accatgcgag ccagcacacg gaaaggggtg
     4801 cacagaagcc tgtccacata cagcgtgagc acagctcccg actggctgag atggatcccc
     4861 tccaagcact gaagagtttt cttcagcatg gttggagttg aaaggttttc acaacgagac
     4921 tgaattgcct ggatgaacag gccgctggca gcagagttcc gatgaacggc actgatgaag
     4981 tcctgtactg gaggctcgtg ggaaaggctg atcagatctt gaatgtgatt tacaatgagc
     5041 cacgttaagt gctcggagtc atggaggttc tgacagacat aatcacagaa gagaatgaga
     5101 gcccctcttc gtactatttc tctattgcac attccaagtt tggctgccaa gtcagaatcc
     5161 tcctcttctc cagacatctg gggactaagt aactttgtgc tggacagact gtgtcttttc
     5221 ggggtctgct gcacttctgc ccaccagcgg tagtcggtgt ggttgacaag cagcagtatc
     5281 tgacaccaga gcagcaccag ggccgggtgg gtggtgatca tggaacgagc ccgcaagttc
     5341 aagctgtcca gggtgtagaa actgccgcca cagccatcac tgcggaacag cctagtggca
     5401 gctgctgtga ttctccggaa cattccagac ttgaagatgt ggatcagaca cattagcagt
     5461 gtgcctagtt cctggcaata gaaagtatgt tgctgctcac tcatttccac cttcagctgt
     5521 tttgtaacaa tgtcttctaa aagaatacca accagttgta atagaaacct tgaaaatgtt
     5581 tcttctggca aattctttat ttgtttccct tcactgtgtt cttctagcgt tgaagtactg
     5641 tccccatctc ttaacctatt aattactgta caggagatta aatacggaga gaaggagagc
     5701 tcctgaatac gagaaagaac aatatcttca gttgactggg aaatcagaac cctcaaaatg
     5761 gccagaattc ccgatatcca cagttgaaca gtgctcacgg acgccattgt gtttggagtg
     5821 acgaacatac tccgtaaaag catgtctacc ggacggaggg aggaaggggc caaaatctca
     5881 aataatgtat ttaacactcc aagggcttca tgagagtcaa tgtgcatctg ctgtttggct
     5941 aacattggga ggatgatgtc agctatctgt cgagacagtc gcttccactt gtcttcattc
     6001 tccttgtggc actgctgcag gacaagaatg aacatctcca acacctgatg gtactggatg
     6061 agtctcagta acattgacac caccacctct ttttgggttt caagctcttt tcctgcatca
     6121 gctttatttg ttcctcttaa tacaaagagg tcgtggacta tgggctgcag agccggtatg
     6181 gcatgtgtca cagccttcct tccactggcc atgatgccat cacagagctg aatgatttta
     6241 ggaattccaa tgatctgttt tgaatgatag cgttcataag atagtaatac caagaagaaa
     6301 aagatgtttg gaatgattgc ctctgattcc ctgaactggc ccacttcaat gtattcaaac
     6361 tgtttcaata caaagccaat aaacacctga tctgaatcca gaagacagta attaacccgt
     6421 aactgaacca gctgcgccag caaatctaaa acctgcttct gtaactgcac acatgttgta
     6481 gtcgtgtact gttttaaagc ttttataaca agaggttcaa acaaacgaat gtgattatga
     6541 atagcattct tatctgcacg gttctttgtg acactcgtga ggtttgtctt caactgggta
     6601 gacactttct ggaggacatc aaaccatccc gaggtgtcgt tctcctgctc cgcctgcacc
     6661 atgttcctca ggctggcgtc agcgagggcc tgggtgaagt gggtgtacgg ggccatgaag
     6721 cagtagtggt acaagcctgg cctcacactg gaggagccaa ggcgctgtgc tcggccttgt
     6781 gacttgctgg ggttggaaga taagccatca aactgggagg ccaagtttgt gccaaagaga
     6841 gtcttcaaca attgttgaac acaaacagtt gccatcattg gttctcgact aaagcaggat
     6901 ttcaggtatc ctaggatctc ttcaacacac ttcccaatgt cctgcagtgt ggccagctct
     6961 agtatctgag aaagaacatc caaggctgag cgaagaaacc ctccaaactt ttccgtgctg
     7021 ttctgaagat ccagcgtgac cttgtagtta gcgtgtgtag ctttcaggac atcatgcagt
     7081 ttgaggtatg aaggaagatg atagaaactc cccagtgatg aggatttact tgttgtaaca
     7141 ggacctgagg tatcagattg tctagaagct gcactggcct cactgccttt cttgggactc
     7201 aacggtacag atgcttgttc tcctggttct ttctccttcc cctttcgtcg gatgggactt
     7261 agagaagggg ggtttgttag agaaggcaag gctgccttta ttgcgggtcc aggagccacg
     7321 tcatccagga cgtgggcaca aatgttaatc accttcagca ggtgagagaa gagctgctcc
     7381 accatgggca ccagggcccg gtcccccagg gctggccaga cctcctcttg cttggtggct
     7441 gctgggttgg cttcttcttc agaggcccat gaacttctca gagatttggg agcactggct
     7501 gcaagcaagt ttccggccaa aatcaaagca tcttgatggg ctgagagatc caatgggaac
     7561 caagctgacg agagcagggt cagaatcatt gtggccatcc caacggtaca gctcttccta
     7621 gactcatctg aggcactcag tggaggcact ccacagtgcc aacctaaact ccaaatgcaa
     7681 actgggaagg cagtggaaag aagacacaaa gcttcacagc atccaaatgt gagtgctctg
     7741 gtggttgatg tgattagttc atgagaaact gctgcaataa ctcttgaaag gttattttcc
     7801 atagtgacgt ctgttatgct tggtagtagg ttatagcctc tatatattct ggttattgtg
     7861 ctgacggaga aatgagatgg aggctgcgtc tcatgcatga gaagtttcag gtaaacactg
     7921 ctttgatctc ttgccacggc cactactgga tcagcttgtc cttggtcaca tttataaaac
     7981 agctttggga caagcctaat tagtgatgct gcggcaacat gtcgcaccct ggggtcttca
     8041 tctccaagca aatggatgac aacattattg agcactcgtt cttgcagttt taaaagccct
     8101 gtataatgat gagcccctct gtgtaagttt tctgcttttg cctccaaaaa gctcaccagc
     8161 ctgaagtcaa tctctgcaag ggtttccaga agctctgtcc tcaccagcca ataggaactg
     8221 ttcctcagag tcagcacatc gatgatcagc tgcagtccta actcactgta gctgctgctg
     8281 cagagactca tgacacagtt cctcacagct gtacaagcta acttgcaagt aacagaagac
     8341 tcatccttca gtgttttccg cagcaaagga atgcaatccg ccaaagaaaa tgtatttcct
     8401 gtgagggttc taatggtgcc catccaatct cccacgtgga agcgggacct gctgaggatg
     8461 gagcagatga gggtcccaca gagaatggca gtggctcctc gaacctgtgg gtctccatga
     8521 tcgatgtagt tcaagatgtc tgagacatac tgttcctcag ggtattccgt ggtgtcaaga
     8581 ggaactttat agagtttgct gaagaaagat tccgggtgga gggccacagc tgctcccaca
     8641 cagctgaggg ccagggcctt cacgctgacc ctcacatccc tgtccggaac cagcacattt
     8701 tttccccctg ttagcaaaaa cgaagcagat aaaaggcgga cacaatggac aagaggtgca
     8761 gagtcatcat cagtggactg tccaatgtca cctttgatgc ggcaaggctt gttttcttga
     8821 tcacccggtt cagtagcttc atctctcaac acaaatttat caacactgct gtcagaaggc
     8881 tgcctgcagt gactcatgtt tttcaataaa tgtgcctgtt gaagggccat ggaagagttc
     8941 ctgaaggcct ccgaggcttc atcaggaaga atacctgtgg cttcctcatc ttcatcctgg
     9001 ggctgtccaa tctgcaggcc caaatactgg ttgtcggtac cgtctaacac aatttcagaa
     9061 ctgtctgaag gggtaacagc tgaatcaggc ccttcggtgg tggtctggga gctgtcgctg
     9121 atgggcgacg aggcctgggt cccatcattc aggtccatgg cagggtcaga tgggacggcg
     9181 ctgacctggc tggagctgtg gctcaagata tcctcctcat ccccatcagt ggcagagctt
     9241 gtcaagtcac agctggccag atccactgag tccgcctgca gtgtgtgctg tgaccgtggc
     9301 tgttctgtga tgatgtcatg acctgctgac cctggagtgg aaacccctga agaagcagcc
     9361 agctctccac tgatctcatc cttcactgag gctgttaagg cagagctgct gacatccgat
     9421 ctcgattcag agtcatcctc caaggcttct tcttctccta agagcacttt gcctttttgt
     9481 tttcttgaaa ggacagggct gcatgaggaa ccccctccag ctataagttc cacaatactc
     9541 ccactacggc ttcggccacc agactcctcc ttagcagcgg tgagctgccc aatgcccccg
     9601 actgcggtca gggtttgcag aagctcgggt ggaggcgttc tgaagagctg ctgcaacagc
     9661 tccagggctc cggtcacaac attgtggtct tggtgctgtg tatgatgtaa cgtcagttca
     9721 taaacctgga caagctgctc tgcagaagga gagacttcca tttctttcct tgtcactccg
     9781 aagctgcctt tcaggcttgt gtccttgacc tgctgctgca gcaagggcac caaatacctc
     9841 agggtgagca gcacgccaag aatcagcaga gtggagtgtt catcctcgac aggaacgagt
     9901 aagcctaaga gcacatttag tagccaacta tagaaatatt gtgtccttct tgagtgctgg
     9961 cagatgctca ctgctgatcc agccgctgtc cgccgaatgg tgggggagct tgacttcagg
    10021 ttcgctatga aggcctttaa caaaacctta atttcattgt catttgcaaa attgccaaaa
    10081 gaagccataa ttttgggaac agctgcagcc aaggtctcct ggactgattc ttcgggtctc
    10141 ttgcttgttc gagtcaggca cggcagaagg ttcaccaggt aaggcctgca tttctgaggc
    10201 cgaaccaggt gagccagctc agcaaacctc cacagggcag cacgcaaact ccgaggggca
    10261 ccattctttt taatttcctt atagagctcg agctgtaacc ttggaagatt agaatccatc
    10321 aaagctttga taactttgtt gaggcattcg tcagccacca tcctgacatc tgactctgcg
    10381 tcatcactgc acagcagaaa aagttccata gcgatgccca gaagtttctg aaattctgga
    10441 gaatttctga cagactgtgc cactatgttt tcacatattg tcagacaatg attcacacgg
    10501 tctttcttgg tagctgaaag ttctttcttt ggtcggtgca gcggctcctc agccacagcc
    10561 ggcatagtat atctccttct taaggatccg tcgagtgatt gtaaataaaa tgtaatttac
    10621 agtatagtat tttaattaat atacaaatga tttgataata attcttattt aactataata
    10681 tattgtgttg ggttgaatta aaggtccgta ttcgataagc cagtaagcag tgggttctct
    10741 agttagccag agagctctgc ttatatagac ctcccaccgt acacgcctac cgcccatttg
    10801 cgtcaatggg gcggagttgt tacgacattt tggaaagtcc cgttgatttt ggtgccaaaa
    10861 caaactccca ttgacgtcaa tggggtggag acttggaaat ccccgtgagt caaaccgcta
    10921 tccacgccca ttgatgtact gccaaaaccg catcaccatg gtaatagcga tgactaatac
    10981 gtagatgtac tgccaagtag gaaagtccca taaggtcatg tactgggcat aatgccaggc
    11041 gggccattta ccgtcattga cgtcaatagg gggcgtactt ggcatatgat acacttgatg
    11101 tactgccaag tgggcagttt accgtaaata ctccacccat tgacgtcaat ggaaagtccc
    11161 tattggcgtt actatgggaa catacgtcat tattgacgtc aatgggcggg ggtcgttggg
    11221 cggtcagcca ggcgggccat ttaccgtaag ttatgtaacg cggaactcca tatatgggct
    11281 atgaactaat gaccccgtaa ttgattacta ttaataacta gtcaataatc aatgtcaacg
    11341 cgtatatctg gcccgtacag atccgcatgc ggtaccggga gatgggggag gctaactgaa
    11401 acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat
    11461 aaaacgcacg ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca
    11521 ctctgtcgat accccaccga gaccccattg ggaccaatac gcccgcgttt cttccttttc
    11581 cccaccccaa cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc
    11641 aagccctgcc atagccacta cgggtacgta gccaaccact agaactatag ctagagtcct
    11701 gggcgaacaa acgatgctcg ccttccagaa aaccgaggat gcgaaccact tcatccgggg
    11761 tcagcaccac cggcaagcgc cgcgacggcc gaggtctacc gatctcctga agcaagggca
    11821 gatccgtgca cagcaccttg ccgtagaaga acagcaaggc cgccaatgcc tgacgatgcg
    11881 tggagaccga aaccttgcgc tcgttcgcca gccaggacag aaatgcctcg acttcgctgc
    11941 tgcccaaggt tgccgggtga cgcacaccgt ggaaacggat gaaggcacga acccagttga
    12001 cataagcctg ttcggttcgt aaactgtaat gcaagtagcg tatgcgctca cgcaactggt
    12061 ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt
    12121 atgactgttt ttttgtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg
    12181 tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagcaa cgatgttacg
    12241 cagcagggca gtcgccctaa aacaaagtta ggtggctcaa gtatgggcat cattcgcaca
    12301 tgtaggctcg gccctgacca agtcaaatcc atgcgggctg ctcttgatct tttcggtcgt
    12361 gagttcggag acgtagccac ctactcccaa catcagccgg actccgatta cctcgggaac
    12421 ttgctccgta gtaagacatt catcgcgctt gctgccttcg accaagaagc ggttgttggc
    12481 gctctcgcgg cttacgttct gcccaagttt gagcagccgc gtagtgagat ctatatctat
    12541 gatctcgcag tctccggcga gcaccggagg cagggcattg ccaccgcgct catcaatctc
    12601 ttgaagcatg aggccaacgc gcttggtgct tatgtgatct acgtgcaagc agattacggt
    12661 gacgatcccg cagtggctct ctatacaaag ttgggcatac gggaagaagt gatgcacttt
    12721 gatatcgacc caagtaccgc cacctaacaa ttcgttcaag ccgagatcgg cttcccggcc
    12781 gcggagttgt tcggtaaatt gtcacaacgc cgcgaatata gtctttacca tgcccttggc
    12841 cacgcccctc tttaatacga cgggcaattt gcacttcaga aaatgaagag tttgctttag
    12901 ccataacaaa agtccagtat gctttttcac agcataactg gactgatttc agtttacaac
    12961 tattctgtct agtttaagac tttattgtca tagtttagat ctattttgtt cagtttaaga
    13021 ctttattgtc cgcccacacc cgcttacgca gggcatccat ttattactca accgtaaccg
    13081 attttgccag gttacgcggc tggtctatgc ggtgtgaaat accgcacaga tgcgtaagga
    13141 gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
    13201 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
    13261 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
    13321 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
    13381 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
    13441 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
    13501 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
    13561 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
    13621 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
    13681 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
    13741 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
    13801 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
    13861 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
    13921 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
    13981 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
    14041 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
    14101 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
    14161 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
    14221 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
    14281 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
    14341 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
    14401 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
    14461 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
    14521 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
    14581 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
    14641 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
    14701 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
    14761 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
    14821 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
    14881 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
    14941 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
    15001 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
    15061 ttccgcgcac atttccccga aaagtgccac ctaaattgta agcgttaata ttttgttaaa
    15121 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa
    15181 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa
    15241 caagagtcca ctattaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag
    15301 ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt
    15361 aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg
    15421 gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca
    15481 agtgtagcgg tcacgctgcg cgtaaccacc aca
//
