LOCUS       sequence-345747-       14767 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Binary vector for Agrobacterium-mediated plant transformation - 
            Promoter:GUS reporter for expression analysis of GOXL genes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14767)
  AUTHORS   Sola K, Dean GH, Li Y, Lohmann J, Movahedan M, Gilchrist EJ, Adams 
            KL, Haughn GW
  TITLE     Expression Patterns and Functional Characterisation of Arabidopsis 
            GALACTOSE OXIDASE-LIKE (GOXL) Genes Suggest Specialised Roles for 
            Galactose Oxidases in Plants.
  JOURNAL   Plant Cell Physiol. 2021 May 27. pii: 6286013. doi: 
            10.1093/pcp/pcab073.
  PUBMED    34042158
REFERENCE   2  (bases 1 to 14767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell 
            Physiol. 2021 May 27. pii: 6286013. doi: 10.1093/pcp/pcab073."
COMMENT     Sequence Label: pBI101.1/ProGOXL4:GUS
FEATURES             Location/Qualifiers
     source          1..14767
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        149..328
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        181..364
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             385..1179
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(439..458)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1049..1068
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      1569..1821
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    2355..2376
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2391..2396,2397..2414,2415..2421)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2429..2445
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2434..2456
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2453..2469
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2453..2469
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3401..5212
                     /codon_start=1
                     /gene="uidA (or gusA) from E. coli"
                     /product="beta-glucuronidase"
                     /label=GUS
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
                     PKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     terminator      5283..5535
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(5544..5561)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5544..5560)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5553..5575)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(5700..5719)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5910..5931
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    6178..6202
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(7798..8946)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(9245..10039)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  10231..10998
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      11621..12332
                     /label=oriV
                     /note="incP origin of replication"
     CDS             complement(12459..12830)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            12863..12972
                     /note="incP origin of transfer"
     primer_bind     13195..13214
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13448..13465
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(13653..14303)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
ORIGIN
        1 gtttacccgc caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac
       61 aatctgatca tgagcggaga attaagggag tcacgttatg acccccgccg atgacgcggg
      121 acaagccgtt ttacgtttgg aactgacaga accgcaacgt tgaaggagcc actcagccgc
      181 gggtttctgg agtttaatga gctaagcaca tacgtcagaa accattattg cgcgttcaaa
      241 agtcgcctaa ggtcactatc agctagcaaa tatttcttgt caaaaatgct ccactgacgt
      301 tccataaatt cccctcggta tccaattaga gtctcatatt cactctcaat ccaaataatc
      361 tgcaccggat ctggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg
      421 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
      481 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
      541 ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg
      601 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
      661 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
      721 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
      781 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
      841 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
      901 aggctcaagg cgcgcatgcc cgacggcgat gatctcgtcg tgacccatgg cgatgcctgc
      961 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
     1021 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
     1081 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
     1141 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa
     1201 tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct
     1261 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
     1321 gggatctcat gctggagttc ttcgcccacg ggatctctgc ggaacaggcg gtcgaaggtg
     1381 ccgatatcat tacgacagca acggccgaca agcacaacgc cacgatcctg agcgacaata
     1441 tgatcgggcc cggcgtccac atcaacggcg tcggcggcga ctgcccaggc aagaccgaga
     1501 tgcaccgcga tatcttgctg cgttcggata ttttcgtgga gttcccgcca cagacccgga
     1561 tgatccccga tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg
     1621 gtcttgcgat gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca
     1681 tgtaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg caattataca
     1741 tttaatacgc gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg
     1801 tgtcatctat gttactagat cgggcctcct gtcaatgctg gcggcggctc tggtggtggt
     1861 tctggtggcg gctctgaggg tggtggctct gagggtggcg gctctgaggg aggcggttcc
     1921 ggtggtggct ctggttccgg tgattttgat tatgaaaaga tggcaaacgc taataagggg
     1981 gctatgaccg aaaatgccga tgaaaacgcg ctacagtctg acgctaaagg caaacttgat
     2041 tctgtcgcta ctgattacgg tgctgctatc gatggtttca ttggtgacgt ttccggcctt
     2101 gctaatggta atggtgctac tggtgatttt gctggctcta attcccaaat ggctcaagtc
     2161 ggtgacggtg ataattcacc tttaatgaat aatttccgtc aatatttacc ttccctccct
     2221 caatcggttg aatgtcgccc ttttgtcttt ggcccaatac gcaaaccgcc tctccccgcg
     2281 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     2341 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
     2401 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
     2461 agctatgacc atgattacgc caagcttgag gaagaccttt tttcaaactg agctcaggtg
     2521 aactaaaatg accattttga cctttacatg aaatatgtaa tgaaattaat ttggttaaac
     2581 cgagactaaa accggtctta acacaaagca agaactaaac tgaatttaaa aagtcagcgt
     2641 tttgttaaca tgccataatt gaatgctgta attcaaaatt tacggagaca ttctgccgag
     2701 tatgtatata tggagacatt cttgaggctg agcaatttac tcccaaagtt ccagcttttt
     2761 tgttttttag attttgatca gccggtctat ggagacattc ttaaggtgat atttttgtaa
     2821 gtgagtgata tttttgtcat tttgatttat aaacatataa gaataaggaa atatttgtct
     2881 gataatttct taagtttttt tttttgggca ttctctttgc aacttgcgga attatagaaa
     2941 tcatcatgca ggtgtacatt gttttctcta ttcatttcat acttatttat tatatgtgtt
     3001 ccttacttat ttatttccaa atattacgac aatttcaaag agtgatgata ccagaattta
     3061 gaaagaaaat attacatcct ttaatttgat taaaactatc tatagtttga tgcaattcta
     3121 ctacgttttc ttaaagactg tctaaaactc cggcatgaat gtagtagagt caaagagttt
     3181 gcttgtccaa aattccaaat taaatatata aaacaagaat cgtcaatcta ataaaaataa
     3241 caattttatg tgaaagaata tataattgga cgtattactc caaccatact actcttcaac
     3301 aagaaaatag ataaaccaac ctctaactta aaattcacca tcaaaccaag aacaaaggaa
     3361 gaaaccacca accactggat ccccgggtgg tcagtccctt atgttacgtc ctgtagaaac
     3421 cccaacccgt gaaatcaaaa aactcgacgg cctgtgggca ttcagtctgg atcgcgaaaa
     3481 ctgtggaatt gatcagcgtt ggtgggaaag cgcgttacaa gaaagccggg caattgctgt
     3541 gccaggcagt tttaacgatc agttcgccga tgcagatatt cgtaattatg cgggcaacgt
     3601 ctggtatcag cgcgaagtct ttataccgaa aggttgggca ggccagcgta tcgtgctgcg
     3661 tttcgatgcg gtcactcatt acggcaaagt gtgggtcaat aatcaggaag tgatggagca
     3721 tcagggcggc tatacgccat ttgaagccga tgtcacgccg tatgttattg ccgggaaaag
     3781 tgtacgtatc accgtttgtg tgaacaacga actgaactgg cagactatcc cgccgggaat
     3841 ggtgattacc gacgaaaacg gcaagaaaaa gcagtcttac ttccatgatt tctttaacta
     3901 tgccggaatc catcgcagcg taatgctcta caccacgccg aacacctggg tggacgatat
     3961 caccgtggtg acgcatgtcg cgcaagactg taaccacgcg tctgttgact ggcaggtggt
     4021 ggccaatggt gatgtcagcg ttgaactgcg tgatgcggat caacaggtgg ttgcaactgg
     4081 acaaggcact agcgggactt tgcaagtggt gaatccgcac ctctggcaac cgggtgaagg
     4141 ttatctctat gaactgtgcg tcacagccaa aagccagaca gagtgtgata tctacccgct
     4201 tcgcgtcggc atccggtcag tggcagtgaa gggcgaacag ttcctgatta accacaaacc
     4261 gttctacttt actggctttg gtcgtcatga agatgcggac ttgcgtggca aaggattcga
     4321 taacgtgctg atggtgcacg accacgcatt aatggactgg attggggcca actcctaccg
     4381 tacctcgcat tacccttacg ctgaagagat gctcgactgg gcagatgaac atggcatcgt
     4441 ggtgattgat gaaactgctg ctgtcggctt taacctctct ttaggcattg gtttcgaagc
     4501 gggcaacaag ccgaaagaac tgtacagcga agaggcagtc aacggggaaa ctcagcaagc
     4561 gcacttacag gcgattaaag agctgatagc gcgtgacaaa aaccacccaa gcgtggtgat
     4621 gtggagtatt gccaacgaac cggatacccg tccgcaaggt gcacgggaat atttcgcgcc
     4681 actggcggaa gcaacgcgta aactcgaccc gacgcgtccg atcacctgcg tcaatgtaat
     4741 gttctgcgac gctcacaccg ataccatcag cgatctcttt gatgtgctgt gcctgaaccg
     4801 ttattacgga tggtatgtcc aaagcggcga tttggaaacg gcagagaagg tactggaaaa
     4861 agaacttctg gcctggcagg agaaactgca tcagccgatt atcatcaccg aatacggcgt
     4921 ggatacgtta gccgggctgc actcaatgta caccgacatg tggagtgaag agtatcagtg
     4981 tgcatggctg gatatgtatc accgcgtctt tgatcgcgtc agcgccgtcg tcggtgaaca
     5041 ggtatggaat ttcgccgatt ttgcgacctc gcaaggcata ttgcgcgttg gcggtaacaa
     5101 gaaagggatc ttcactcgcg accgcaaacc gaagtcggcg gcttttctgc tgcaaaaacg
     5161 ctggactggc atgaacttcg gtgaaaaacc gcagcaggga ggcaaacaat gaatcaacaa
     5221 ctctcctggc gcaccatcgt cggctacagc ctcgggaatt gctaccgagc tcgaatttcc
     5281 ccgatcgttc aaacatttgg caataaagtt tcttaagatt gaatcctgtt gccggtcttg
     5341 cgatgattat catataattt ctgttgaatt acgttaagca tgtaataatt aacatgtaat
     5401 gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta tacatttaat
     5461 acgcgataga aaacaaaata tagcgcgcaa actaggataa attatcgcgc gcggtgtcat
     5521 ctatgttact agatcgggaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     5581 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     5641 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgcccgc
     5701 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     5761 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     5821 acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     5881 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     5941 caaccctatc tcgggctatt cttttgattt ataagggatt ttgccgattt cggaaccacc
     6001 atcaaacagg attttcgcct gctggggcaa accagcgtgg accgcttgct gcaactctct
     6061 cagggccagg cggtgaaggg caatcagctg ttgcccgtct cactggtgaa aagaaaaacc
     6121 accccagtac attaaaaacg tccgcaatgt gttattaagt tgtctaagcg tcaatttgtt
     6181 tacaccacaa tatatcctgc caccagccag ccaacagctc cccgaccggc agctcggcac
     6241 aaaatcacca ctcgatacag gcagcccatc agtccgggac ggcgtcagcg ggagagccgt
     6301 tgtaaggcgg cagactttgc tcatgttacc gatgctattc ggaagaacgg caactaagct
     6361 gccgggtttg aaacacggat gatctcgcgg agggtagcat gttgattgta acgatgacag
     6421 agcgttgctg cctgtgatca aatatcatct ccctcgcaga gatccgaatt atcagccttc
     6481 ttattcattt ctcgcttaac cgtgacaggc tgtcgatctt gagaactatg ccgacataat
     6541 aggaaatcgc tggataaagc cgctgaggaa gctgagtggc gctatttctt tagaagtgaa
     6601 cgttgacgat atcaactccc ctatccattg ctcaccgaat ggtacaggtc ggggacccga
     6661 agttccgact gtcggcctga tgcatccccg gctgatcgac cccagatctg gggctgagaa
     6721 agcccagtaa ggaaacaact gtaggttcga gtcgcgagat cccccggaac caaaggaagt
     6781 aggttaaacc cgctccgatc aggccgagcc acgccaggcc gagaacattg gttcctgtag
     6841 gcatcgggat tggcggatca aacactaaag ctactggaac gagcagaagt cctccggccg
     6901 ccagttgcca ggcggtaaag gtgagcagag gcacgggagg ttgccacttg cgggtcagca
     6961 cggttccgaa cgccatggaa accgcccccg ccaggcccgc tgcgacgccg acaggatcta
     7021 gcgctgcgtt tggtgtcaac accaacagcg ccacgcccgc agttccgcaa atagccccca
     7081 ggaccgccat caatcgtatc gggctaccta gcagagcggc agagatgaac acgaccatca
     7141 gcggctgcac agcgcctacc gtcgccgcga ccccgcccgg caggcggtag accgaaataa
     7201 acaacaagct ccagaatagc gaaatattaa gtgcgccgag gatgaagatg cgcatccacc
     7261 agattcccgt tggaatctgt cggacgatca tcacgagcaa taaacccgcc ggcaacgccc
     7321 gcagcagcat accggcgacc cctcggcctc gctgttcggg ctccacgaaa acgccggaca
     7381 gatgcgcctt gtgagcgtcc ttggggccgt cctcctgttt gaagaccgac agcccaatga
     7441 tctcgccgtc gatgtaggcg ccgaatgcca cggcatctcg caaccgttca gcgaacgcct
     7501 ccatgggctt tttctcctcg tgctcgtaaa cggacccgaa catctctgga gctttcttca
     7561 gggccgacaa tcggatctcg cggaaatcct gcacgtcggc cgctccaagc cgtcgaatct
     7621 gagccttaat cacaattgtc aattttaatc ctctgtttat cggcagttcg tagagcgcgc
     7681 cgtgcgtccc gagcgatact gagcgaagca agtgcgtcga gcagtgcccg cttgttcctg
     7741 aaatgccagt aaagcgctgg ctgctgaacc cccagccgga actgacccca caaggcccta
     7801 gcgtttgcaa tgcaccaggt catcattgac ccaggcgtgt tccaccaggc cgctgcctcg
     7861 caactcttcg caggcttcgc cgacctgctc gcgccacttc ttcacgcggg tggaatccga
     7921 tccgcacatg aggcggaagg tttccagctt gagcgggtac ggctcccggt gcgagctgaa
     7981 atagtcgaac atccgtcggg ccgtcggcga cagcttgcgg tacttctccc atatgaattt
     8041 cgtgtagtgg tcgccagcaa acagcacgac gatttcctcg tcgatcagga cctggcaacg
     8101 ggacgttttc ttgccacggt ccaggacgcg gaagcggtgc agcagcgaca ccgattccag
     8161 gtgcccaacg cggtcggacg tgaagcccat cgccgtcgcc tgtaggcgcg acaggcattc
     8221 ctcggccttc gtgtaatacc ggccattgat cgaccagccc aggtcctggc aaagctcgta
     8281 gaacgtgaag gtgatcggct cgccgatagg ggtgcgcttc gcgtactcca acacctgctg
     8341 ccacaccagt tcgtcatcgt cggcccgcag ctcgacgccg gtgtaggtga tcttcacgtc
     8401 cttgttgacg tggaaaatga ccttgttttg cagcgcctcg cgcgggattt tcttgttgcg
     8461 cgtggtgaac agggcagagc gggccgtgtc gtttggcatc gctcgcatcg tgtccggcca
     8521 cggcgcaata tcgaacaagg aaagctgcat ttccttgatc tgctgcttcg tgtgtttcag
     8581 caacgcggcc tgcttggcct cgctgacctg ttttgccagg tcctcgccgg cggtttttcg
     8641 cttcttggtc gtcatagttc ctcgcgtgtc gatggtcatc gacttcgcca aacctgccgc
     8701 ctcctgttcg agacgacgcg aacgctccac ggcggccgat ggcgcgggca gggcaggggg
     8761 agccagttgc acgctgtcgc gctcgatctt ggccgtagct tgctggacca tcgagccgac
     8821 ggactggaag gtttcgcggg gcgcacgcat gacggtgcgg cttgcgatgg tttcggcatc
     8881 ctcggcggaa aaccccgcgt cgatcagttc ttgcctgtat gccttccggt caaacgtccg
     8941 attcattcac cctccttgcg ggattgcccc gactcacgcc ggggcaatgt gcccttattc
     9001 ctgatttgac ccgcctggtg ccttggtgtc cagataatcc accttatcgg caatgaagtc
     9061 ggtcccgtag accgtctggc cgtccttctc gtacttggta ttccgaatct tgccctgcac
     9121 gaataccagc gaccccttgc ccaaatactt gccgtgggcc tcggcctgag agccaaaaca
     9181 cttgatgcgg aagaagtcgg tgcgctcctg cttgtcgccg gcatcgttgc gccacatcta
     9241 ggtactaaaa caattcatcc agtaaaatat aatattttat tttctcccaa tcaggcttga
     9301 tccccagtaa gtcaaaaaat agctcgacat actgttcttc cccgatatcc tccctgatcg
     9361 accggacgca gaaggcaatg tcataccact tgtccgccct gccgcttctc ccaagatcaa
     9421 taaagccact tactttgcca tctttcacaa agatgttgct gtctcccagg tcgccgtggg
     9481 aaaagacaag ttcctcttcg ggcttttccg tctttaaaaa atcatacagc tcgcgcggat
     9541 ctttaaatgg agtgtcttct tcccagtttt cgcaatccac atcggccaga tcgttattca
     9601 gtaagtaatc caattcggct aagcggctgt ctaagctatt cgtataggga caatccgata
     9661 tgtcgatgga gtgaaagagc ctgatgcact ccgcatacag ctcgataatc ttttcagggc
     9721 tttgttcatc ttcatactct tccgagcaaa ggacgccatc ggcctcactc atgagcagat
     9781 tgctccagcc atcatgccgt tcaaagtgca ggacctttgg aacaggcagc tttccttcca
     9841 gccatagcat catgtccttt tcccgttcca catcataggt ggtcccttta taccggctgt
     9901 ccgtcatttt taaatatagg ttttcatttt ctcccaccag cttatatacc ttagcaggag
     9961 acattccttc cgtatctttt acgcagcggt atttttcgat cagttttttc aattccggtg
    10021 atattctcat tttagccatt tattatttcc ttcctctttt ctacagtatt taaagatacc
    10081 ccaagaagct aattataaca agacgaactc caattcactg ttccttgcat tctaaaacct
    10141 taaataccag aaaacagctt tttcaaagtt gttttcaaag ttggcgtata acatagtatc
    10201 gacggagccg attttgaaac cacaattatg ggtgatgctg ccaacttact gatttagtgt
    10261 atgatggtgt ttttgaggtg ctccagtggc ttctgtttct atcagctgtc cctcctgttc
    10321 agctactgac ggggtggtgc gtaacggcaa aagcaccgcc ggacatcagc gctatctctg
    10381 ctctcactgc cgtaaaacat ggcaactgca gttcacttac accgcttctc aacccggtac
    10441 gcaccagaaa atcattgata tggccatgaa tggcgttgga tgccgggcaa cagcccgcat
    10501 tatgggcgtt ggcctcaaca cgattttacg tcacttaaaa aactcaggcc gcagtcggta
    10561 acctcgcgca tacagccggg cagtgacgtc atcgtctgcg cggaaatgga cgaacagtgg
    10621 ggctatgtcg gggctaaatc gcgccagcgc tggctgtttt acgcgtatga cagtctccgg
    10681 aagacggttg ttgcgcacgt attcggtgaa cgcactatgg cgacgctggg gcgtcttatg
    10741 agcctgctgt caccctttga cgtggtgata tggatgacgg atggctggcc gctgtatgaa
    10801 tcccgcctga agggaaagct gcacgtaatc agcaagcgat atacgcagcg aattgagcgg
    10861 cataacctga atctgaggca gcacctggca cggctgggac ggaagtcgct gtcgttctca
    10921 aaatcggtgg agctgcatga caaagtcatc gggcattatc tgaacataaa acactatcaa
    10981 taagttggag tcattaccca attatgatag aatttacaag ctataaggtt attgtcctgg
    11041 gtttcaagca ttagtccatg caagttttta tgctttgccc attctataga tatattgata
    11101 agcgcgctgc ctatgccttg ccccctgaaa tccttacata cggcgatatc ttctatataa
    11161 aagatatatt atcttatcag tattgtcaat atattcaagg caatctgcct cctcatcctc
    11221 ttcatcctct tcgtcttggt agctttttaa atatggcgct tcatagagta attctgtaaa
    11281 ggtccaattc tcgttttcat acctcggtat aatcttacct atcacctcaa atggttcgct
    11341 gggtttatcg cacccccgaa cacgagcacg gcacccgcga ccactatgcc aagaatgccc
    11401 aaggtaaaaa ttgccggccc cgccatgaag tccgtgaatg ccccgacggc cgaagtgaag
    11461 ggcaggccgc cacccaggcc gccgccctca ctgcccggca cctggtcgct gaatgtcgat
    11521 gccagcacct gcggcacgtc aatgcttccg ggcgtcgcgc tcgggctgat cgcccatccc
    11581 gttactgccc cgatcccggc aatggcaagg actgccagcg ctgccatttt tggggtgagg
    11641 ccgttcgcgg ccgaggggcg cagcccctgg ggggatggga ggcccgcgtt agcgggccgg
    11701 gagggttcga gaaggggggg cacccccctt cggcgtgcgc ggtcacgcgc acagggcgca
    11761 gccctggtta aaaacaaggt ttataaatat tggtttaaaa gcaggttaaa agacaggtta
    11821 gcggtggccg aaaaacgggc ggaaaccctt gcaaatgctg gattttctgc ctgtggacag
    11881 cccctcaaat gtcaataggt gcgcccctca tctgtcagca ctctgcccct caagtgtcaa
    11941 ggatcgcgcc cctcatctgt cagtagtcgc gcccctcaag tgtcaatacc gcagggcact
    12001 tatccccagg cttgtccaca tcatctgtgg gaaactcgcg taaaatcagg cgttttcgcc
    12061 gatttgcgag gctggccagc tccacgtcgc cggccgaaat cgagcctgcc cctcatctgt
    12121 caacgccgcg ccgggtgagt cggcccctca agtgtcaacg tccgcccctc atctgtcagt
    12181 gagggccaag ttttccgcga ggtatccaca acgccggcgg ccgcggtgtc tcgcacacgg
    12241 cttcgacggc gtttctggcg cgtttgcagg gccatagacg gccgccagcc cagcggcgag
    12301 ggcaaccagc ccggtgagcg tcggaaaggc gctcggtctt gccttgctcg tcggtgatgt
    12361 acttcaccag ctccgcgaag tcgctcttct tgatggagcg catggggacg tgcttggcaa
    12421 tcacgcgcac cccccggccg ttttagcggc taaaaaagtc atggctctgc cctcgggcgg
    12481 accacgccca tcatgacctt gccaagctcg tcctgcttct cttcgatctt cgccagcagg
    12541 gcgaggatcg tggcatcacc gaaccgcgcc gtgcgcgggt cgtcggtgag ccagagtttc
    12601 agcaggccgc ccaggcggcc caggtcgcca ttgatgcggg ccagctcgcg gacgtgctca
    12661 tagtccacga cgcccgtgat tttgtagccc tggccgacgg ccagcaggta ggccgacagg
    12721 ctcatgccgg ccgccgccgc cttttcctca atcgctcttc gttcgtctgg aaggcagtac
    12781 accttgatag gtgggctgcc cttcctggtt ggcttggttt catcagccat ccgcttgccc
    12841 tcatctgtta cgccggcggt agccggccag cctcgcagag caggattccc gttgagcacc
    12901 gccaggtgcg aataagggac agtgaagaag gaacacccgc tcgcgggtgg gcctacttca
    12961 cctatcctgc ccggctgacg ccgttggata caccaaggaa agtctacacg aaccctttgg
    13021 caaaatcctg tatatcgtgc gaaaaaggat ggatataccg aaaaaatcgc tataatgacc
    13081 ccgaagcagg gttatgcagc ggaaaagcgc cacgcttccc gaagggagaa aggcggacag
    13141 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
    13201 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
    13261 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
    13321 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
    13381 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
    13441 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc cagaaggccg ccagagaggc
    13501 cgagcgcggc cgtgaggctt ggacgctagg gcagggcatg aaaaagcccg tagcgggctg
    13561 ctacgggcgt ctgacgcggt ggaaaggggg aggggatgtt gtctacatgg ctctgctgta
    13621 gtgagtgggt tgcgctccgg cagcggtcct gatcaatcgt caccctttct cggtccttca
    13681 acgttcctga caacgagcct ccttttcgcc aatccatcga caatcaccgc gagtccctgc
    13741 tcgaacgctg cgtccggacc ggcttcgtcg aaggcgtcta tcgcggcccg caacagcggc
    13801 gagagcggag cctgttcaac ggtgccgccg cgctcgccgg catcgctgtc gccggcctgc
    13861 tcctcaagca cggccccaac agtgaagtag ctgattgtca tcagcgcatt gacggcgtcc
    13921 ccggccgaaa aacccgcctc gcagaggaag cgaagctgcg cgtcggccgt ttccatctgc
    13981 ggtgcgcccg gtcgcgtgcc ggcatggatg cgcgcgccat cgcggtaggc gagcagcgcc
    14041 tgcctgaagc tgcgggcatt cccgatcaga aatgagcgcc agtcgtcgtc ggctctcggc
    14101 accgaatgcg tatgattctc cgccagcatg gcttcggcca gtgcgtcgag cagcgcccgc
    14161 ttgttcctga agtgccagta aagcgccggc tgctgaaccc ccaaccgttc cgccagtttg
    14221 cgtgtcgtca gaccgtctac gccgacctcg ttcaacaggt ccagggcggc acggatcact
    14281 gtattcggct gcaactttgt catgcttgac actttatcac tgataaacat aatatgtcca
    14341 ccaacttatc agtgataaag aatccgcgcg ttcaatcgga ccagcggagg ctggtccgga
    14401 ggccagacgt gaaacccaac atacccctga tcgtaattct gagcactgtc gcgctcgacg
    14461 ctgtcggcat cggcctgatt atgccggtgc tgccgggcct cctgcgcgat ctggttcact
    14521 cgaacgacgt caccgcccac tatggcattc tgctggcgct gtatgcgttg gtgcaatttg
    14581 cctgcgcacc tgtgctgggc gcgctgtcgg atcgtttcgg gcggcggcca atcttgctcg
    14641 tctcgctggc cggcgccaga tctggggaac cctgtggttg gcatgcacat acaaatggac
    14701 gaacggataa accttttcac gcccttttaa atatccgatt attctaataa acgctctttt
    14761 ctcttag
//
