LOCUS       sequence-345750-       14981 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Binary vector for Agrobacterium-mediated plant transformation - 
            Promoter:GUS reporter for expression analysis of GOXL genes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14981)
  AUTHORS   Sola K, Dean GH, Li Y, Lohmann J, Movahedan M, Gilchrist EJ, Adams 
            KL, Haughn GW
  TITLE     Expression Patterns and Functional Characterisation of Arabidopsis 
            GALACTOSE OXIDASE-LIKE (GOXL) Genes Suggest Specialised Roles for 
            Galactose Oxidases in Plants.
  JOURNAL   Plant Cell Physiol. 2021 May 27. pii: 6286013. doi: 
            10.1093/pcp/pcab073.
  PUBMED    34042158
REFERENCE   2  (bases 1 to 14981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell 
            Physiol. 2021 May 27. pii: 6286013. doi: 10.1093/pcp/pcab073."
COMMENT     Sequence Label: pBI101.1/ProGOXL6:GUS
FEATURES             Location/Qualifiers
     source          1..14981
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        149..328
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        181..364
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             385..1179
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(439..458)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1049..1068
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      1569..1821
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    2355..2376
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2391..2396,2397..2414,2415..2421)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2429..2445
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2434..2456
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2453..2469
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2453..2469
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3615..5426
                     /codon_start=1
                     /gene="uidA (or gusA) from E. coli"
                     /product="beta-glucuronidase"
                     /label=GUS
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
                     PKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     terminator      5497..5749
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(5758..5775)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5758..5774)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5767..5789)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(5914..5933)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6124..6145
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    6392..6416
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(8012..9160)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(9459..10253)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  10445..11212
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      11835..12546
                     /label=oriV
                     /note="incP origin of replication"
     CDS             complement(12673..13044)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            13077..13186
                     /note="incP origin of transfer"
     primer_bind     13409..13428
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13662..13679
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(13867..14517)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
ORIGIN
        1 gtttacccgc caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac
       61 aatctgatca tgagcggaga attaagggag tcacgttatg acccccgccg atgacgcggg
      121 acaagccgtt ttacgtttgg aactgacaga accgcaacgt tgaaggagcc actcagccgc
      181 gggtttctgg agtttaatga gctaagcaca tacgtcagaa accattattg cgcgttcaaa
      241 agtcgcctaa ggtcactatc agctagcaaa tatttcttgt caaaaatgct ccactgacgt
      301 tccataaatt cccctcggta tccaattaga gtctcatatt cactctcaat ccaaataatc
      361 tgcaccggat ctggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg
      421 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
      481 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
      541 ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg
      601 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
      661 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
      721 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
      781 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
      841 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
      901 aggctcaagg cgcgcatgcc cgacggcgat gatctcgtcg tgacccatgg cgatgcctgc
      961 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
     1021 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
     1081 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
     1141 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa
     1201 tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct
     1261 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
     1321 gggatctcat gctggagttc ttcgcccacg ggatctctgc ggaacaggcg gtcgaaggtg
     1381 ccgatatcat tacgacagca acggccgaca agcacaacgc cacgatcctg agcgacaata
     1441 tgatcgggcc cggcgtccac atcaacggcg tcggcggcga ctgcccaggc aagaccgaga
     1501 tgcaccgcga tatcttgctg cgttcggata ttttcgtgga gttcccgcca cagacccgga
     1561 tgatccccga tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg
     1621 gtcttgcgat gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca
     1681 tgtaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg caattataca
     1741 tttaatacgc gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg
     1801 tgtcatctat gttactagat cgggcctcct gtcaatgctg gcggcggctc tggtggtggt
     1861 tctggtggcg gctctgaggg tggtggctct gagggtggcg gctctgaggg aggcggttcc
     1921 ggtggtggct ctggttccgg tgattttgat tatgaaaaga tggcaaacgc taataagggg
     1981 gctatgaccg aaaatgccga tgaaaacgcg ctacagtctg acgctaaagg caaacttgat
     2041 tctgtcgcta ctgattacgg tgctgctatc gatggtttca ttggtgacgt ttccggcctt
     2101 gctaatggta atggtgctac tggtgatttt gctggctcta attcccaaat ggctcaagtc
     2161 ggtgacggtg ataattcacc tttaatgaat aatttccgtc aatatttacc ttccctccct
     2221 caatcggttg aatgtcgccc ttttgtcttt ggcccaatac gcaaaccgcc tctccccgcg
     2281 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     2341 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
     2401 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
     2461 agctatgacc atgattacgc caagcttaga aagccatgaa attagtgtac ataaataact
     2521 aactaatgga gattacttgt attttctaaa accgaagtat attacggaaa tccagactaa
     2581 tctcaagaga catgtaaaaa cccggtattc ttgtcactac tggtgtttac cgtggccaaa
     2641 aggttaacta caatgatttt catagagagt gttactcgat cctcgacttt gacatgtatg
     2701 gtgacttggt gagtcctcga caaattcaag agaccattgg aacacacact attggtcacg
     2761 actatattac gagaaagatt gaatgttatg gagttaagga taatatgtat ttagaagtct
     2821 ttgattatgt aagttagatt gttgttagtt ttcaatgcat ggtgttttat gtttacacta
     2881 tgtttgataa agaaagttta tgttcttaaa ctacaattac tttaagtaca atcttgttca
     2941 ctttgaaaaa taatagggtc ctacacaatt atacgtcacc gcaattattt atctttatag
     3001 acattgctgt tttcagtttc ataaagacta acactagata tatagtcact tccctcaaca
     3061 aggaaaacaa ttatcaacgt cgggacagat ctactctgat gcttgttgat gtgcaagtcc
     3121 actgcagcca attaaatgtt aacaatgtcc gtgacatgca tagtcattat tgttaagttg
     3181 gctcatttgg gaatgtgtta atcatctatt aattatgtta gttgagagtt tgttgccaat
     3241 cacgtttctt tattactttt caaaatatgt gtaatatgta tagttatgtg tacgtattat
     3301 gcaatccttt accctttgga tcagccccat gatggtttgt aaggaaatgg tccttaactt
     3361 tatgacatca aattaagaaa ttgtctgcat gaccacgtca ttgtaaaaaa aagggcacga
     3421 gagacagata cgaaacacca atgttttctt ctatcacaga aactccacaa atctataggc
     3481 tgttttgggg agacgtcttc ttaccacaca tttaaaaata gcaattgcta ataataaacg
     3541 taagtgaaat aacaacacct ataaatcacg cagagacaag agcttgttct ggatccccgg
     3601 gtggtcagtc ccttatgtta cgtcctgtag aaaccccaac ccgtgaaatc aaaaaactcg
     3661 acggcctgtg ggcattcagt ctggatcgcg aaaactgtgg aattgatcag cgttggtggg
     3721 aaagcgcgtt acaagaaagc cgggcaattg ctgtgccagg cagttttaac gatcagttcg
     3781 ccgatgcaga tattcgtaat tatgcgggca acgtctggta tcagcgcgaa gtctttatac
     3841 cgaaaggttg ggcaggccag cgtatcgtgc tgcgtttcga tgcggtcact cattacggca
     3901 aagtgtgggt caataatcag gaagtgatgg agcatcaggg cggctatacg ccatttgaag
     3961 ccgatgtcac gccgtatgtt attgccggga aaagtgtacg tatcaccgtt tgtgtgaaca
     4021 acgaactgaa ctggcagact atcccgccgg gaatggtgat taccgacgaa aacggcaaga
     4081 aaaagcagtc ttacttccat gatttcttta actatgccgg aatccatcgc agcgtaatgc
     4141 tctacaccac gccgaacacc tgggtggacg atatcaccgt ggtgacgcat gtcgcgcaag
     4201 actgtaacca cgcgtctgtt gactggcagg tggtggccaa tggtgatgtc agcgttgaac
     4261 tgcgtgatgc ggatcaacag gtggttgcaa ctggacaagg cactagcggg actttgcaag
     4321 tggtgaatcc gcacctctgg caaccgggtg aaggttatct ctatgaactg tgcgtcacag
     4381 ccaaaagcca gacagagtgt gatatctacc cgcttcgcgt cggcatccgg tcagtggcag
     4441 tgaagggcga acagttcctg attaaccaca aaccgttcta ctttactggc tttggtcgtc
     4501 atgaagatgc ggacttgcgt ggcaaaggat tcgataacgt gctgatggtg cacgaccacg
     4561 cattaatgga ctggattggg gccaactcct accgtacctc gcattaccct tacgctgaag
     4621 agatgctcga ctgggcagat gaacatggca tcgtggtgat tgatgaaact gctgctgtcg
     4681 gctttaacct ctctttaggc attggtttcg aagcgggcaa caagccgaaa gaactgtaca
     4741 gcgaagaggc agtcaacggg gaaactcagc aagcgcactt acaggcgatt aaagagctga
     4801 tagcgcgtga caaaaaccac ccaagcgtgg tgatgtggag tattgccaac gaaccggata
     4861 cccgtccgca aggtgcacgg gaatatttcg cgccactggc ggaagcaacg cgtaaactcg
     4921 acccgacgcg tccgatcacc tgcgtcaatg taatgttctg cgacgctcac accgatacca
     4981 tcagcgatct ctttgatgtg ctgtgcctga accgttatta cggatggtat gtccaaagcg
     5041 gcgatttgga aacggcagag aaggtactgg aaaaagaact tctggcctgg caggagaaac
     5101 tgcatcagcc gattatcatc accgaatacg gcgtggatac gttagccggg ctgcactcaa
     5161 tgtacaccga catgtggagt gaagagtatc agtgtgcatg gctggatatg tatcaccgcg
     5221 tctttgatcg cgtcagcgcc gtcgtcggtg aacaggtatg gaatttcgcc gattttgcga
     5281 cctcgcaagg catattgcgc gttggcggta acaagaaagg gatcttcact cgcgaccgca
     5341 aaccgaagtc ggcggctttt ctgctgcaaa aacgctggac tggcatgaac ttcggtgaaa
     5401 aaccgcagca gggaggcaaa caatgaatca acaactctcc tggcgcacca tcgtcggcta
     5461 cagcctcggg aattgctacc gagctcgaat ttccccgatc gttcaaacat ttggcaataa
     5521 agtttcttaa gattgaatcc tgttgccggt cttgcgatga ttatcatata atttctgttg
     5581 aattacgtta agcatgtaat aattaacatg taatgcatga cgttatttat gagatgggtt
     5641 tttatgatta gagtcccgca attatacatt taatacgcga tagaaaacaa aatatagcgc
     5701 gcaaactagg ataaattatc gcgcgcggtg tcatctatgt tactagatcg ggaattcact
     5761 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
     5821 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
     5881 ttcccaacag ttgcgcagcc tgaatggcgc ccgctccttt cgctttcttc ccttcctttc
     5941 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     6001 gatttagtgc tttacggcac ctcgacccca aaaaacttga tttgggtgat ggttcacgta
     6061 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     6121 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcgggc tattcttttg
     6181 atttataagg gattttgccg atttcggaac caccatcaaa caggattttc gcctgctggg
     6241 gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca
     6301 gctgttgccc gtctcactgg tgaaaagaaa aaccacccca gtacattaaa aacgtccgca
     6361 atgtgttatt aagttgtcta agcgtcaatt tgtttacacc acaatatatc ctgccaccag
     6421 ccagccaaca gctccccgac cggcagctcg gcacaaaatc accactcgat acaggcagcc
     6481 catcagtccg ggacggcgtc agcgggagag ccgttgtaag gcggcagact ttgctcatgt
     6541 taccgatgct attcggaaga acggcaacta agctgccggg tttgaaacac ggatgatctc
     6601 gcggagggta gcatgttgat tgtaacgatg acagagcgtt gctgcctgtg atcaaatatc
     6661 atctccctcg cagagatccg aattatcagc cttcttattc atttctcgct taaccgtgac
     6721 aggctgtcga tcttgagaac tatgccgaca taataggaaa tcgctggata aagccgctga
     6781 ggaagctgag tggcgctatt tctttagaag tgaacgttga cgatatcaac tcccctatcc
     6841 attgctcacc gaatggtaca ggtcggggac ccgaagttcc gactgtcggc ctgatgcatc
     6901 cccggctgat cgaccccaga tctggggctg agaaagccca gtaaggaaac aactgtaggt
     6961 tcgagtcgcg agatcccccg gaaccaaagg aagtaggtta aacccgctcc gatcaggccg
     7021 agccacgcca ggccgagaac attggttcct gtaggcatcg ggattggcgg atcaaacact
     7081 aaagctactg gaacgagcag aagtcctccg gccgccagtt gccaggcggt aaaggtgagc
     7141 agaggcacgg gaggttgcca cttgcgggtc agcacggttc cgaacgccat ggaaaccgcc
     7201 cccgccaggc ccgctgcgac gccgacagga tctagcgctg cgtttggtgt caacaccaac
     7261 agcgccacgc ccgcagttcc gcaaatagcc cccaggaccg ccatcaatcg tatcgggcta
     7321 cctagcagag cggcagagat gaacacgacc atcagcggct gcacagcgcc taccgtcgcc
     7381 gcgaccccgc ccggcaggcg gtagaccgaa ataaacaaca agctccagaa tagcgaaata
     7441 ttaagtgcgc cgaggatgaa gatgcgcatc caccagattc ccgttggaat ctgtcggacg
     7501 atcatcacga gcaataaacc cgccggcaac gcccgcagca gcataccggc gacccctcgg
     7561 cctcgctgtt cgggctccac gaaaacgccg gacagatgcg ccttgtgagc gtccttgggg
     7621 ccgtcctcct gtttgaagac cgacagccca atgatctcgc cgtcgatgta ggcgccgaat
     7681 gccacggcat ctcgcaaccg ttcagcgaac gcctccatgg gctttttctc ctcgtgctcg
     7741 taaacggacc cgaacatctc tggagctttc ttcagggccg acaatcggat ctcgcggaaa
     7801 tcctgcacgt cggccgctcc aagccgtcga atctgagcct taatcacaat tgtcaatttt
     7861 aatcctctgt ttatcggcag ttcgtagagc gcgccgtgcg tcccgagcga tactgagcga
     7921 agcaagtgcg tcgagcagtg cccgcttgtt cctgaaatgc cagtaaagcg ctggctgctg
     7981 aacccccagc cggaactgac cccacaaggc cctagcgttt gcaatgcacc aggtcatcat
     8041 tgacccaggc gtgttccacc aggccgctgc ctcgcaactc ttcgcaggct tcgccgacct
     8101 gctcgcgcca cttcttcacg cgggtggaat ccgatccgca catgaggcgg aaggtttcca
     8161 gcttgagcgg gtacggctcc cggtgcgagc tgaaatagtc gaacatccgt cgggccgtcg
     8221 gcgacagctt gcggtacttc tcccatatga atttcgtgta gtggtcgcca gcaaacagca
     8281 cgacgatttc ctcgtcgatc aggacctggc aacgggacgt tttcttgcca cggtccagga
     8341 cgcggaagcg gtgcagcagc gacaccgatt ccaggtgccc aacgcggtcg gacgtgaagc
     8401 ccatcgccgt cgcctgtagg cgcgacaggc attcctcggc cttcgtgtaa taccggccat
     8461 tgatcgacca gcccaggtcc tggcaaagct cgtagaacgt gaaggtgatc ggctcgccga
     8521 taggggtgcg cttcgcgtac tccaacacct gctgccacac cagttcgtca tcgtcggccc
     8581 gcagctcgac gccggtgtag gtgatcttca cgtccttgtt gacgtggaaa atgaccttgt
     8641 tttgcagcgc ctcgcgcggg attttcttgt tgcgcgtggt gaacagggca gagcgggccg
     8701 tgtcgtttgg catcgctcgc atcgtgtccg gccacggcgc aatatcgaac aaggaaagct
     8761 gcatttcctt gatctgctgc ttcgtgtgtt tcagcaacgc ggcctgcttg gcctcgctga
     8821 cctgttttgc caggtcctcg ccggcggttt ttcgcttctt ggtcgtcata gttcctcgcg
     8881 tgtcgatggt catcgacttc gccaaacctg ccgcctcctg ttcgagacga cgcgaacgct
     8941 ccacggcggc cgatggcgcg ggcagggcag ggggagccag ttgcacgctg tcgcgctcga
     9001 tcttggccgt agcttgctgg accatcgagc cgacggactg gaaggtttcg cggggcgcac
     9061 gcatgacggt gcggcttgcg atggtttcgg catcctcggc ggaaaacccc gcgtcgatca
     9121 gttcttgcct gtatgccttc cggtcaaacg tccgattcat tcaccctcct tgcgggattg
     9181 ccccgactca cgccggggca atgtgccctt attcctgatt tgacccgcct ggtgccttgg
     9241 tgtccagata atccacctta tcggcaatga agtcggtccc gtagaccgtc tggccgtcct
     9301 tctcgtactt ggtattccga atcttgccct gcacgaatac cagcgacccc ttgcccaaat
     9361 acttgccgtg ggcctcggcc tgagagccaa aacacttgat gcggaagaag tcggtgcgct
     9421 cctgcttgtc gccggcatcg ttgcgccaca tctaggtact aaaacaattc atccagtaaa
     9481 atataatatt ttattttctc ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg
     9541 acatactgtt cttccccgat atcctccctg atcgaccgga cgcagaaggc aatgtcatac
     9601 cacttgtccg ccctgccgct tctcccaaga tcaataaagc cacttacttt gccatctttc
     9661 acaaagatgt tgctgtctcc caggtcgccg tgggaaaaga caagttcctc ttcgggcttt
     9721 tccgtcttta aaaaatcata cagctcgcgc ggatctttaa atggagtgtc ttcttcccag
     9781 ttttcgcaat ccacatcggc cagatcgtta ttcagtaagt aatccaattc ggctaagcgg
     9841 ctgtctaagc tattcgtata gggacaatcc gatatgtcga tggagtgaaa gagcctgatg
     9901 cactccgcat acagctcgat aatcttttca gggctttgtt catcttcata ctcttccgag
     9961 caaaggacgc catcggcctc actcatgagc agattgctcc agccatcatg ccgttcaaag
    10021 tgcaggacct ttggaacagg cagctttcct tccagccata gcatcatgtc cttttcccgt
    10081 tccacatcat aggtggtccc tttataccgg ctgtccgtca tttttaaata taggttttca
    10141 ttttctccca ccagcttata taccttagca ggagacattc cttccgtatc ttttacgcag
    10201 cggtattttt cgatcagttt tttcaattcc ggtgatattc tcattttagc catttattat
    10261 ttccttcctc ttttctacag tatttaaaga taccccaaga agctaattat aacaagacga
    10321 actccaattc actgttcctt gcattctaaa accttaaata ccagaaaaca gctttttcaa
    10381 agttgttttc aaagttggcg tataacatag tatcgacgga gccgattttg aaaccacaat
    10441 tatgggtgat gctgccaact tactgattta gtgtatgatg gtgtttttga ggtgctccag
    10501 tggcttctgt ttctatcagc tgtccctcct gttcagctac tgacggggtg gtgcgtaacg
    10561 gcaaaagcac cgccggacat cagcgctatc tctgctctca ctgccgtaaa acatggcaac
    10621 tgcagttcac ttacaccgct tctcaacccg gtacgcacca gaaaatcatt gatatggcca
    10681 tgaatggcgt tggatgccgg gcaacagccc gcattatggg cgttggcctc aacacgattt
    10741 tacgtcactt aaaaaactca ggccgcagtc ggtaacctcg cgcatacagc cgggcagtga
    10801 cgtcatcgtc tgcgcggaaa tggacgaaca gtggggctat gtcggggcta aatcgcgcca
    10861 gcgctggctg ttttacgcgt atgacagtct ccggaagacg gttgttgcgc acgtattcgg
    10921 tgaacgcact atggcgacgc tggggcgtct tatgagcctg ctgtcaccct ttgacgtggt
    10981 gatatggatg acggatggct ggccgctgta tgaatcccgc ctgaagggaa agctgcacgt
    11041 aatcagcaag cgatatacgc agcgaattga gcggcataac ctgaatctga ggcagcacct
    11101 ggcacggctg ggacggaagt cgctgtcgtt ctcaaaatcg gtggagctgc atgacaaagt
    11161 catcgggcat tatctgaaca taaaacacta tcaataagtt ggagtcatta cccaattatg
    11221 atagaattta caagctataa ggttattgtc ctgggtttca agcattagtc catgcaagtt
    11281 tttatgcttt gcccattcta tagatatatt gataagcgcg ctgcctatgc cttgccccct
    11341 gaaatcctta catacggcga tatcttctat ataaaagata tattatctta tcagtattgt
    11401 caatatattc aaggcaatct gcctcctcat cctcttcatc ctcttcgtct tggtagcttt
    11461 ttaaatatgg cgcttcatag agtaattctg taaaggtcca attctcgttt tcatacctcg
    11521 gtataatctt acctatcacc tcaaatggtt cgctgggttt atcgcacccc cgaacacgag
    11581 cacggcaccc gcgaccacta tgccaagaat gcccaaggta aaaattgccg gccccgccat
    11641 gaagtccgtg aatgccccga cggccgaagt gaagggcagg ccgccaccca ggccgccgcc
    11701 ctcactgccc ggcacctggt cgctgaatgt cgatgccagc acctgcggca cgtcaatgct
    11761 tccgggcgtc gcgctcgggc tgatcgccca tcccgttact gccccgatcc cggcaatggc
    11821 aaggactgcc agcgctgcca tttttggggt gaggccgttc gcggccgagg ggcgcagccc
    11881 ctggggggat gggaggcccg cgttagcggg ccgggagggt tcgagaaggg ggggcacccc
    11941 ccttcggcgt gcgcggtcac gcgcacaggg cgcagccctg gttaaaaaca aggtttataa
    12001 atattggttt aaaagcaggt taaaagacag gttagcggtg gccgaaaaac gggcggaaac
    12061 ccttgcaaat gctggatttt ctgcctgtgg acagcccctc aaatgtcaat aggtgcgccc
    12121 ctcatctgtc agcactctgc ccctcaagtg tcaaggatcg cgcccctcat ctgtcagtag
    12181 tcgcgcccct caagtgtcaa taccgcaggg cacttatccc caggcttgtc cacatcatct
    12241 gtgggaaact cgcgtaaaat caggcgtttt cgccgatttg cgaggctggc cagctccacg
    12301 tcgccggccg aaatcgagcc tgcccctcat ctgtcaacgc cgcgccgggt gagtcggccc
    12361 ctcaagtgtc aacgtccgcc cctcatctgt cagtgagggc caagttttcc gcgaggtatc
    12421 cacaacgccg gcggccgcgg tgtctcgcac acggcttcga cggcgtttct ggcgcgtttg
    12481 cagggccata gacggccgcc agcccagcgg cgagggcaac cagcccggtg agcgtcggaa
    12541 aggcgctcgg tcttgccttg ctcgtcggtg atgtacttca ccagctccgc gaagtcgctc
    12601 ttcttgatgg agcgcatggg gacgtgcttg gcaatcacgc gcaccccccg gccgttttag
    12661 cggctaaaaa agtcatggct ctgccctcgg gcggaccacg cccatcatga ccttgccaag
    12721 ctcgtcctgc ttctcttcga tcttcgccag cagggcgagg atcgtggcat caccgaaccg
    12781 cgccgtgcgc gggtcgtcgg tgagccagag tttcagcagg ccgcccaggc ggcccaggtc
    12841 gccattgatg cgggccagct cgcggacgtg ctcatagtcc acgacgcccg tgattttgta
    12901 gccctggccg acggccagca ggtaggccga caggctcatg ccggccgccg ccgccttttc
    12961 ctcaatcgct cttcgttcgt ctggaaggca gtacaccttg ataggtgggc tgcccttcct
    13021 ggttggcttg gtttcatcag ccatccgctt gccctcatct gttacgccgg cggtagccgg
    13081 ccagcctcgc agagcaggat tcccgttgag caccgccagg tgcgaataag ggacagtgaa
    13141 gaaggaacac ccgctcgcgg gtgggcctac ttcacctatc ctgcccggct gacgccgttg
    13201 gatacaccaa ggaaagtcta cacgaaccct ttggcaaaat cctgtatatc gtgcgaaaaa
    13261 ggatggatat accgaaaaaa tcgctataat gaccccgaag cagggttatg cagcggaaaa
    13321 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
    13381 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
    13441 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
    13501 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
    13561 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
    13621 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
    13681 aagcggaaga gcgccagaag gccgccagag aggccgagcg cggccgtgag gcttggacgc
    13741 tagggcaggg catgaaaaag cccgtagcgg gctgctacgg gcgtctgacg cggtggaaag
    13801 ggggagggga tgttgtctac atggctctgc tgtagtgagt gggttgcgct ccggcagcgg
    13861 tcctgatcaa tcgtcaccct ttctcggtcc ttcaacgttc ctgacaacga gcctcctttt
    13921 cgccaatcca tcgacaatca ccgcgagtcc ctgctcgaac gctgcgtccg gaccggcttc
    13981 gtcgaaggcg tctatcgcgg cccgcaacag cggcgagagc ggagcctgtt caacggtgcc
    14041 gccgcgctcg ccggcatcgc tgtcgccggc ctgctcctca agcacggccc caacagtgaa
    14101 gtagctgatt gtcatcagcg cattgacggc gtccccggcc gaaaaacccg cctcgcagag
    14161 gaagcgaagc tgcgcgtcgg ccgtttccat ctgcggtgcg cccggtcgcg tgccggcatg
    14221 gatgcgcgcg ccatcgcggt aggcgagcag cgcctgcctg aagctgcggg cattcccgat
    14281 cagaaatgag cgccagtcgt cgtcggctct cggcaccgaa tgcgtatgat tctccgccag
    14341 catggcttcg gccagtgcgt cgagcagcgc ccgcttgttc ctgaagtgcc agtaaagcgc
    14401 cggctgctga acccccaacc gttccgccag tttgcgtgtc gtcagaccgt ctacgccgac
    14461 ctcgttcaac aggtccaggg cggcacggat cactgtattc ggctgcaact ttgtcatgct
    14521 tgacacttta tcactgataa acataatatg tccaccaact tatcagtgat aaagaatccg
    14581 cgcgttcaat cggaccagcg gaggctggtc cggaggccag acgtgaaacc caacataccc
    14641 ctgatcgtaa ttctgagcac tgtcgcgctc gacgctgtcg gcatcggcct gattatgccg
    14701 gtgctgccgg gcctcctgcg cgatctggtt cactcgaacg acgtcaccgc ccactatggc
    14761 attctgctgg cgctgtatgc gttggtgcaa tttgcctgcg cacctgtgct gggcgcgctg
    14821 tcggatcgtt tcgggcggcg gccaatcttg ctcgtctcgc tggccggcgc cagatctggg
    14881 gaaccctgtg gttggcatgc acatacaaat ggacgaacgg ataaaccttt tcacgccctt
    14941 ttaaatatcc gattattcta ataaacgctc ttttctctta g
//
