LOCUS       sequence-345844-        3111 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SED1 biosensor for expression in Escherichia coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Cuevas-Velazquez CL, Vellosillo T, Guadalupe K, Schmidt HB, Yu F, 
            Moses D, Brophy JAN, Cosio-Acosta D, Das A, Wang L, Jones AM, 
            Covarrubias AA, Sukenik S, Dinneny JR
  TITLE     Intrinsically disordered protein biosensor tracks the 
            physical-chemical effects of osmotic stress on cells.
  JOURNAL   Nat Commun. 2021 Sep 14;12(1):5438. doi: 10.1038/s41467-021-25736-8.
  PUBMED    34521831
REFERENCE   2  (bases 1 to 3111)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-09-14"; volume: "12(1):5438. doi"; pages: " 
            10.1038/s41467-021-25736-8"
COMMENT     Sequence Label: pDEST-HisMBP-SED1
FEATURES             Location/Qualifiers
     source          1..3111
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             10..27
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     regulatory      976..985
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     1093..1116
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             1168..1188
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2703..2714
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 atgaaaatcc atcaccatca ccatcacgaa gaaggtaaac tggtaatctg gattaacggc
       61 gataaaggct ataacggtct cgctgaagtc ggtaagaaat tcgagaaaga taccggaatt
      121 aaagtcaccg ttgagcatcc ggataaactg gaagagaaat tcccacaggt tgcggcaact
      181 ggcgatggcc ctgacattat cttctgggca cacgaccgct ttggtggcta cgctcaatct
      241 ggcctgttgg ctgaaatcac cccggacaaa gcgttccagg acaagctgta tccgtttacc
      301 tgggatgccg tacgttacaa cggcaagctg attgcttacc cgatcgctgt tgaagcgtta
      361 tcgctgattt ataacaaaga tctgctgccg aacccgccaa aaacctggga agagatcccg
      421 gcgctggata aagaactgaa agcgaaaggt aagagcgcgc tgatgttcaa cctgcaagaa
      481 ccgtacttca cctggccgct gattgctgct gacgggggtt atgcgttcaa gtatgaaaac
      541 ggcaagtacg acattaaaga cgtgggcgtg gataacgctg gcgcgaaagc gggtctgacc
      601 ttcctggttg acctgattaa aaacaaacac atgaatgcag acaccgatta ctccatcgca
      661 gaagctgcct ttaataaagg cgaaacagcg atgaccatca acggcccgtg ggcatggtcc
      721 aacatcgaca ccagcaaagt gaattatggt gtaacggtac tgccgacctt caagggtcaa
      781 ccatccaaac cgttcgttgg cgtgctgagc gcaggtatta acgccgccag tccgaacaaa
      841 gagctggcaa aagagttcct cgaaaactat ctgctgactg atgaaggtct ggaagcggtt
      901 aataaagaca aaccgctggg tgccgtagcg ctgaagtctt acgaggaaga gttggcgaaa
      961 gatccacgta ttgccgccac catggaaaac gcccagaaag gtgaaatcat gccgaacatc
     1021 ccgcagatgt ccgctttctg gtatgccgtg cgtactgcgg tgatcaacgc cgccagcggt
     1081 cgtcagactg tcgatgaagc cctgaaagac gcgcagacta attcgatcac aagtttgtac
     1141 aaaggctccg cggccgcccc cttcaccgag aacttgtact tccaaggtat ggtgagcaaa
     1201 ggtgaagaac tgtttaccgg tgttgttccg attctggttg aactggatgg tgacgttaat
     1261 ggtcacaaat tttcagttag cggtgaaggc gaaggtgatg caacctatgg taaactgacc
     1321 ctgaaattta tctgtaccac cggcaaactg ccggttccgt ggccgaccct ggttaccacc
     1381 ctgagctggg gtgttcagtg ttttgcacgt tatccggatc acatgaaaca gcacgatttt
     1441 ttcaaaagcg caatgccgga aggttatgtt caagaacgta ccatcttctt caaagatgac
     1501 ggcaactata aaacccgtgc cgaagttaaa tttgaaggtg ataccctggt gaatcgcatt
     1561 gaactgaaag gcatcgattt taaagaggat ggtaatatcc tgggccacaa actggaatat
     1621 aatgccattc atggcaacgt gtatatcacc gcagataaac agaaaaacgg catcaaagca
     1681 aattttggcc tgaactgcaa tattgaagat ggttcagttc agctggcaga tcattatcag
     1741 cagaataccc cgattggtga tggtccggtt ctgctgccgg ataatcatta tctgagcacc
     1801 cagagcaaac tgagcaaaga tccgaatgaa aaacgtgatc acatggtgct gctggaattt
     1861 gttaccgcag caggtattac cctgggtatg gatgaactgt ataaagagct catgcagtcg
     1921 atgaaagaaa cagcttcgaa tattgcagct tctgcaaaat ctggcatgga caaaaccaaa
     1981 gctaccttgg aggaaaaggc ggagaagatg aagacacgag accctgttca gaaacagatg
     2041 gctacacagg ttaaagaaga taagatcaat caagctgaga tgcagaagag agaaacgcgt
     2101 cagcacaacg cggccatgaa agaagcggct ggagccggaa ccggtttagg tttggggacg
     2161 gccactcact cgaccactgg acaagtcgga cacggcactg ggacacatca gatgtcggct
     2221 ctgcctggtc acggaacggg acaactgacc gaccgcgttg tggagggcac ggctgtgacc
     2281 gacccgattg gaaggaacac tggaactggt cggacaaccg ctcataacac acacgttggt
     2341 ggtggtggtg ccaccgggta cggaaccggc gggggatata ctggaagatc tatggtgtca
     2401 aaaggtgagg aactgtttac aggcgtggtg ccgatcctgg tagagctgga cggggatgtg
     2461 aatggccata aattcagcgt ttcaggtgaa ggtgagggcg acgccacgta cggaaaactg
     2521 acactgaaat tcatttgcac aacaggtaaa ctgcctgtgc cttggcctac actggtgacc
     2581 acctttggtt atggtctgat gtgctttgct cgctatcctg accacatgaa acaacatgat
     2641 ttctttaaat ctgccatgcc tgaaggctac gtgcaagagc gcaccatttt tttcaaagac
     2701 gatgggaatt acaaaacacg tgcggaggtg aaattcgagg gcgatacact ggttaaccgt
     2761 atcgagctga aaggtatcga cttcaaagag gacggaaaca ttctgggtca taaactggaa
     2821 tacaactaca acagccataa cgtgtacatc atggccgaca aacaaaaaaa cgggattaaa
     2881 gtgaacttca aaatccgcca caacatcgaa gatggcagcg tgcagctggc cgaccactat
     2941 caacaaaaca caccgatcgg cgacggtcct gtactgctgc ctgacaacca ctatctgtca
     3001 tatcagagcg cactgtcaaa agatcctaac gagaaacgcg accacatggt tctgctggaa
     3061 ttcgtgacag ccgctggcat tacactgggc atggacgagc tgtacaaata a
//
