LOCUS       sequence-345865-        6236 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6236)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 6236)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0929:Dendra
FEATURES             Location/Qualifiers
     source          1..6236
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     646..665
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        646..664
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2780..2803
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3060..3860
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3985..4573
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4474..4493
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgccaagatc ggtcggccgg aactccgcat ccacgacctc cgcgctgtcg gcgctacgtt
       61 cgccgctcag gcaggtgcga cgaccaagga gctgatggcc cgtctcggtc acacgactcc
      121 taggatggcg atgaagtacc agatggcgtc tgaggcccgc gacgaggcta tcgctgaggc
      181 gatgtccaag ctggccaaga cctcctgaaa cgcaaaaagc ccccctccca aggacactga
      241 gtcctaaaga ggggggtttc ttgtcagtac gcgaagaacc acgcctggcc gcgagcgcca
      301 gcaccgccgc tctgtgcgga gacctgggca ccagccccgc cgccgccagg agcattgccg
      361 ttcccgccag aaatctagac ggtgaccaca acgcgcccgc tttgatcggg gacgtcgaaa
      421 tattggatcg tcggcaccgt cacggccgtg ggaggcggca cgatccgcga cgtgatgatc
      481 ggccgcatcc ccacggtgct gcgcagtgag ctctacgcca tcccggcgtt gatctgtgcg
      541 ttcgcacgca caggcccggt gtgagaaggg tctctgacga gcgggagaac tccctatcag
      601 tgatagagtt tgtcctccct atcagtgata gataggctta aggagtaata cgactcacta
      661 tagggagaag gaggccatta tgagccccgc cggtgatgac caggccgaga tatcggccga
      721 cagacaggcc ccagcgccgt tgatgaaccg gcggcgcgcc ctgacggcac tcgcggtggg
      781 catgggtgca ggcgtgctgg ccgcgtgctc cggcaagacg ccgctgagca gccaggacga
      841 gcccgaggcc gcacccgccg agccgacgct caagtacagc cccgagcccg acaccgtcga
      901 cgtactgccc acgtccgaag tgggcatcga gatctccgac ggctggatcc agaagatcgc
      961 gctgaccaac gcctcgggca aggtcgtcag cggcaggctc aaccaggacc gcacggcgtt
     1021 caccatcacc gaaccgctcg gctacgacgc cacctacacc tggtccgggt cggtggtcgg
     1081 tgaggacggc aagaccgtgc ccatcgaggg caagttcacc acggtgaccc cgcagaccca
     1141 ggtcagcggt caattccagc tcgccgacgg ccaggtggtc ggcgtcgccg cgccgatcat
     1201 cctgcagttc gacgcctcga tcgacgacca gtaccgcgcc accgtcgaac gggcgctcag
     1261 cgtcgtcacc gaaccgccgg tcgagggcag ctgggcctgg ctgcccgacg aggccgccgg
     1321 atcgcgcgtg cactggcgca gccgcgagta cttcccggcc ggcaccaagg tccacgtcaa
     1381 ggcgccgctg tacggcgtcg agttcggcga cggcgcctac ggtgcggccg atgccaccct
     1441 cgacttcgag atcgggcgca agcaggtggt caaggccgag gcgtcgtcgc accgcatcca
     1501 ggtgctcgac ggcgcgggcg cggtcatcat ggacttcccg tgcagctacg gcgagggcga
     1561 cctggaccgc aacgtcacgc gcagcggcat ccacgtggtc accgagaagt acgaggactt
     1621 ctacatgacc aaccccgcgg ccggctacgc caacgtgcac gagcggttcg cggtgcggat
     1681 ctccaacaac ggcgagttca tccactgcaa cccgaacagc atcggcgcgc agggcaacac
     1741 caacgtcacc aacggttgca tcaacctgaa cctggagaac tcacagcagt acttcaacag
     1801 cgccatctac ggcgacccgg tagaggtcac cggaacccgc atccagctgt cctacgccga
     1861 cggcgacatc tgggactggg ccgtggactg ggacgagtgg aagtcgatgt cggcgttgtc
     1921 cgatacgcgc gcgccgtccg acatcccggc gtcggcgccc gcgacgccga gcgacgcccc
     1981 gacgttgtcg gggacgccga cgacaacggc gccgcccgag cctgcgcccg gcggaaagct
     2041 tggtgcggcc gcgggcaaca cgcctggcat caatctgatc aaggaggaca tgcgtgtcaa
     2101 ggtgcatatg gagggcaacg tgaacggtca cgccttcgtg atcgaggggg agggtaaggg
     2161 taagccttac gaagggaccc agaccgccaa cctcaccgtc aaggagggcg cccctctccc
     2221 attctcgtac gatatcctca ctaccgcggt gcattacggg aaccgcgtgt tcaccaagta
     2281 ccccgaggat atccctgact acttcaagca gagcttcccg gagggatact cctgggagcg
     2341 gacgatgacc ttcgaagaca agggtatctg tacgatccgc agcgacatct cgctggaagg
     2401 ggactgtttc ttccagaacg tccgtttcaa ggggaccaac tttccgccga acggcccggt
     2461 catgcagaag aagaccctca agtgggagcc gagcacggag aagctgcacg ttcgggacgg
     2521 cctcctggtc ggcaacatca acatggcgct gctgctggaa ggcggcggcc actacctatg
     2581 cgacttcaag accacgtaca aagcgaagaa ggtcgtccag ctgcccgacg cgcacttcgt
     2641 ggaccaccgc atcgaaatcc tggggaacga ttcggactac aacaaggtca agttgtacga
     2701 gcacgctgtg gcacgctata gcccgctccc gtcgcaggtc tggttcgaag gcggcggcgg
     2761 ggagaacctg tatttccagg actacaagga cgacgacgac aagtgattta aataacccct
     2821 tggggcctct aaacgggtct tgaggggttt tttgttaact agcgtacgat cgaattcctg
     2881 cagctcacgg taactgatgc cgtatttgca gtaccagcgt acggcccaca gaatgatgtc
     2941 acgctgaaaa tgccggcctt tgaatgggtt catgtgcagc tccatcagca aaaggggatg
     3001 ataagtttat caccaccgac tatttgcaac agtgccgttg atcgtgctat gatcgactga
     3061 tgtcatcagc ggtggagtgc aatgtcgtgc aatacgaatg gcgaaaagcc gagctcatcg
     3121 gtcagcttct caaccttggg gttacccccg gcggtgtgct gctggtccac agctccttcc
     3181 gtagcgtccg gcccctcgaa gatgggccac ttggactgat cgaggccctg cgtgctacgc
     3241 tgggtccggg agggacgctc gtcatgccct cgtggtcagg tctggacgac gagccgttcg
     3301 atcctgccac gtcgcccgtt acaccggacc ttggagttgt ctctgacaca ttctggcgcc
     3361 tgccaaatgt aaagcgcagc gcccatccat ttgcctttgc ggcagcgggg ccacaggcag
     3421 agcagatcat ctctgatcca ttgcccctgc caccttactc gcctgcaagc ccggtcgccc
     3481 gtgtccatga actcgatggg caggtacttc tcctcggcgt gggacacgat gccaacacga
     3541 cgctgcatct tgccgagttg atggcaaagg ttccctatgg ggtgccgaga cactgcacca
     3601 ttcttcagga tggcaagttg gtacgcgtcg attatctcga gaatgaccac tgctgtgagc
     3661 gctttgcctt ggcggacagg tggctcaagg agaagagcct tcagaaggaa ggtccagtcg
     3721 gtcatgcctt tgctcggttg atccgctccc gcgacattgt ggcgacagcc ctgggtcaac
     3781 tgggccgaga tccgttgatc ttcctgcatc cgccagaggc gggatgcgaa gaatgcgatg
     3841 ccgctcgcca gtcgattggc tgagctcatg agcggagaac gagatgacgt tggaggggca
     3901 aggtcgcgct gattgctggg gcaacacggg ggatccacta gttccatgag cgtcagaccc
     3961 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     4021 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     4081 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     4141 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     4201 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     4261 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     4321 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     4381 agaaagcgcc acgcttcccg aggggagaaa ggcggacagg tatccggtaa gcggcagggt
     4441 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     4501 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     4561 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     4621 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     4681 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcaacg cgtgcggccg
     4741 cggtacccgg ggatcctcta gagtcgacca ccaagggcac catctctgct tgggccaccc
     4801 cgttggccgc agccagctcg ctgagagccg tgaacgacag ggcgaacgcc agcccgccga
     4861 cggcgagggt tccgaccgct gcaactcccg gtgcaacctt gtcccggtct attctcttca
     4921 ctgcaccagc tccaatctgg tgtgaatgcc cctcgtctgt tcgcgcaggc ggggggctct
     4981 attcgtttgt cagcatcgaa agtagccaga tcagggatgc gttgcaaccg cgtatgccca
     5041 ggtcagaaga gtcgcacaag agttgcagac ccctggaaag aaaaatggcc agagggcgaa
     5101 aacaccctct gaccagcgga gcgggcgacg ggaatcgaac ccgcgtagct agtttggaag
     5161 aatgggtgtc tgccgaccac atatgggccg gtcaagatag gtttttaccc cctctcggct
     5221 gcatcctcta agtggaaaga aattgcaggt cgtagaagcg cgttgaagcc tgagagttgc
     5281 acaggagttg caacccggta gccttgttca cgacgagagg agacctagtt ggcacgtcgc
     5341 ggatggggat cgctgaagac tcagcgcagc gggaggatcc aagcctcata cgtcaacccg
     5401 caggacggtg tgaggtacta cgcgctgcag acctacgaca acaagatgga cgccgaagcc
     5461 tggctcgcgg gcgagaagcg gctcatcgag atggagacct ggacccctcc acaggaccgg
     5521 gcgaagaagg cagccgccag cgccatcacg ctggaggagt acacccggaa gtggctcgtg
     5581 gagcgcgacc tcgcagacgg caccagggat ctgtacagcg ggcacgcgga gcgccgcatc
     5641 tacccggtgc taggtgaagt ggcggtcaca gagatgacgc cagctctggt gcgtgcgtgg
     5701 tgggccggga tgggtaggaa gcacccgact gcccgccggc atgcctacaa cgtcctccgg
     5761 gcggtgatga acacagcggt cgaggacaag ctgatcgcag agaacccgtg ccggatcgag
     5821 cagaaggcag ccgatgagcg cgacgtagag gcgctgacgc ctgaggagct ggacatcgtc
     5881 gccgctgaga tcttcgagca ctaccggatc gcggcataca tcctggcgtg gacgagcctc
     5941 cggttcggag agctgatcga gcttcgccgc aaggacatcg tggacgacgg catgacgatg
     6001 aagctccggg tgcgccgtgg cgcttcccgc gtggggaaca agatcgtcgt tggcaacgcc
     6061 aagaccgtcc ggtcgaagcg tcctgtgacg gttccgcctc acgtcgcgga gatgatccga
     6121 gcgcacatga aggaccgtac gaagatgaac aagggccccg aggcattcct ggtgaccacg
     6181 acgcagggca accggctgtc gaagtccgcg ttcaccaagt cgctgaagcg tggcta
//
