LOCUS       sequence-345869-        5726 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5726)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5726)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_1046:Dendra
FEATURES             Location/Qualifiers
     source          1..5726
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     749..768
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        749..767
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2373..2396
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2653..3453
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3578..4166
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4067..4086
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 accgtacgaa gatgaacaag ggccccgagg cattcctggt gaccacgacg cagggcaacc
       61 ggctgtcgaa gtccgcgttc accaagtcgc tgaagcgtgg ctacgccaag atcggtcggc
      121 cggaactccg catccacgac ctccgcgctg tcggcgctac gttcgccgct caggcaggtg
      181 cgacgaccaa ggagctgatg gcccgtctcg gtcacacgac tcctaggatg gcgatgaagt
      241 accagatggc gtctgaggcc cgcgacgagg ctatcgctga ggcgatgtcc aagctggcca
      301 agacctcctg aaacgcaaaa agcccccctc ccaaggacac tgagtcctaa agaggggggt
      361 ttcttgtcag tacgcgaaga accacgcctg gccgcgagcg ccagcaccgc cgctctgtgc
      421 ggagacctgg gcaccagccc cgccgccgcc aggagcattg ccgttcccgc cagaaatcta
      481 gacggtgacc acaacgcgcc cgctttgatc ggggacgtcg aaatattgga tcgtcggcac
      541 cgtcacggcc gtgggaggcg gcacgatccg cgacgtgatg atcggccgca tccccacggt
      601 gctgcgcagt gagctctacg ccatcccggc gttgatctgt gcgttcgcac gcacaggccc
      661 ggtgtgagaa gggtctctga cgagcgggag aactccctat cagtgataga gtttgtcctc
      721 cctatcagtg atagataggc ttaaggagta atacgactca ctatagggag aaggaggcca
      781 ttatgcccac aaccggagaa gacgcagccg acaacccaga cctgctgatc gacttcgcca
      841 gggtcaccct gaggcgcggg ggcaacaccc tcgtcgggcc catcacctgg gctgtcgaac
      901 tcgacgagcg ctgggtcgtc atcggtccca acggcgcagg caagacctcg ctgttgcgca
      961 tcgccgcggc gaccgaacac ccgtcgtcgg gaaccgccta cgtactgggg gagcggctgg
     1021 gccgcaccga catggccgaa ctgcgttcgc gcgtcggcct gagcagctcg gctctgtcgc
     1081 aacggattcc cgacgacgag atcgtccgcg acctcgtcgt gtcggccggg tacgcggtgc
     1141 tcgggcggtg gcgcgagcag tacgacgacg tcgactacga gcaggccgtg aacatgctcg
     1201 aaagcgtcgg cgccgagcac ctcgccgaac gccggtacgg caccctgtcg gagggcgaac
     1261 gcaaacgcgt cctgatcgcc cggtcgctca tgaccgaccc ggaactcctg ctgctcgacg
     1321 agcccgcggc cgggctcgac ctcggcggcc gcgaggacct cgtggcccgt ctcaccgacc
     1381 tcgccgccga ccccgatgca ccggccatgg tgctcgtgac gcaccatgtc gaggagatcc
     1441 cggtcgggtt cagccacggc ttgatcctgt ccgagggcaa ggtggtggcc tccggtctgc
     1501 tcaccgatgt gctgacgtcc gagaacctgt ccaaggcatt cggccaggcc atcgccctcg
     1561 aggtgatcga cggacggtac ttcgcgcgcc gggtgaggag ccgggcagcg cacaggaggc
     1621 gaaatgacaa gcttggtgcg gccgcgggca acacgcctgg catcaatctg atcaaggagg
     1681 acatgcgtgt caaggtgcat atggagggca acgtgaacgg tcacgccttc gtgatcgagg
     1741 gggagggtaa gggtaagcct tacgaaggga cccagaccgc caacctcacc gtcaaggagg
     1801 gcgcccctct cccattctcg tacgatatcc tcactaccgc ggtgcattac gggaaccgcg
     1861 tgttcaccaa gtaccccgag gatatccctg actacttcaa gcagagcttc ccggagggat
     1921 actcctggga gcggacgatg accttcgaag acaagggtat ctgtacgatc cgcagcgaca
     1981 tctcgctgga aggggactgt ttcttccaga acgtccgttt caaggggacc aactttccgc
     2041 cgaacggccc ggtcatgcag aagaagaccc tcaagtggga gccgagcacg gagaagctgc
     2101 acgttcggga cggcctcctg gtcggcaaca tcaacatggc gctgctgctg gaaggcggcg
     2161 gccactacct atgcgacttc aagaccacgt acaaagcgaa gaaggtcgtc cagctgcccg
     2221 acgcgcactt cgtggaccac cgcatcgaaa tcctggggaa cgattcggac tacaacaagg
     2281 tcaagttgta cgagcacgct gtggcacgct atagcccgct cccgtcgcag gtctggttcg
     2341 aaggcggcgg cggggagaac ctgtatttcc aggactacaa ggacgacgac gacaagtgat
     2401 ttaaataacc ccttggggcc tctaaacggg tcttgagggg ttttttgtta actagcgtac
     2461 gatcgaattc ctgcagctca cggtaactga tgccgtattt gcagtaccag cgtacggccc
     2521 acagaatgat gtcacgctga aaatgccggc ctttgaatgg gttcatgtgc agctccatca
     2581 gcaaaagggg atgataagtt tatcaccacc gactatttgc aacagtgccg ttgatcgtgc
     2641 tatgatcgac tgatgtcatc agcggtggag tgcaatgtcg tgcaatacga atggcgaaaa
     2701 gccgagctca tcggtcagct tctcaacctt ggggttaccc ccggcggtgt gctgctggtc
     2761 cacagctcct tccgtagcgt ccggcccctc gaagatgggc cacttggact gatcgaggcc
     2821 ctgcgtgcta cgctgggtcc gggagggacg ctcgtcatgc cctcgtggtc aggtctggac
     2881 gacgagccgt tcgatcctgc cacgtcgccc gttacaccgg accttggagt tgtctctgac
     2941 acattctggc gcctgccaaa tgtaaagcgc agcgcccatc catttgcctt tgcggcagcg
     3001 gggccacagg cagagcagat catctctgat ccattgcccc tgccacctta ctcgcctgca
     3061 agcccggtcg cccgtgtcca tgaactcgat gggcaggtac ttctcctcgg cgtgggacac
     3121 gatgccaaca cgacgctgca tcttgccgag ttgatggcaa aggttcccta tggggtgccg
     3181 agacactgca ccattcttca ggatggcaag ttggtacgcg tcgattatct cgagaatgac
     3241 cactgctgtg agcgctttgc cttggcggac aggtggctca aggagaagag ccttcagaag
     3301 gaaggtccag tcggtcatgc ctttgctcgg ttgatccgct cccgcgacat tgtggcgaca
     3361 gccctgggtc aactgggccg agatccgttg atcttcctgc atccgccaga ggcgggatgc
     3421 gaagaatgcg atgccgctcg ccagtcgatt ggctgagctc atgagcggag aacgagatga
     3481 cgttggaggg gcaaggtcgc gctgattgct ggggcaacac gggggatcca ctagttccat
     3541 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     3601 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     3661 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     3721 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     3781 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     3841 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     3901 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     3961 agcgtgagct atgagaaagc gccacgcttc ccgaggggag aaaggcggac aggtatccgg
     4021 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     4081 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     4141 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     4201 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     4261 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     4321 acgcgtgcgg ccgcggtacc cggggatcct ctagagtcga ccaccaaggg caccatctct
     4381 gcttgggcca ccccgttggc cgcagccagc tcgctgagag ccgtgaacga cagggcgaac
     4441 gccagcccgc cgacggcgag ggttccgacc gctgcaactc ccggtgcaac cttgtcccgg
     4501 tctattctct tcactgcacc agctccaatc tggtgtgaat gcccctcgtc tgttcgcgca
     4561 ggcggggggc tctattcgtt tgtcagcatc gaaagtagcc agatcaggga tgcgttgcaa
     4621 ccgcgtatgc ccaggtcaga agagtcgcac aagagttgca gacccctgga aagaaaaatg
     4681 gccagagggc gaaaacaccc tctgaccagc ggagcgggcg acgggaatcg aacccgcgta
     4741 gctagtttgg aagaatgggt gtctgccgac cacatatggg ccggtcaaga taggttttta
     4801 ccccctctcg gctgcatcct ctaagtggaa agaaattgca ggtcgtagaa gcgcgttgaa
     4861 gcctgagagt tgcacaggag ttgcaacccg gtagccttgt tcacgacgag aggagaccta
     4921 gttggcacgt cgcggatggg gatcgctgaa gactcagcgc agcgggagga tccaagcctc
     4981 atacgtcaac ccgcaggacg gtgtgaggta ctacgcgctg cagacctacg acaacaagat
     5041 ggacgccgaa gcctggctcg cgggcgagaa gcggctcatc gagatggaga cctggacccc
     5101 tccacaggac cgggcgaaga aggcagccgc cagcgccatc acgctggagg agtacacccg
     5161 gaagtggctc gtggagcgcg acctcgcaga cggcaccagg gatctgtaca gcgggcacgc
     5221 ggagcgccgc atctacccgg tgctaggtga agtggcggtc acagagatga cgccagctct
     5281 ggtgcgtgcg tggtgggccg ggatgggtag gaagcacccg actgcccgcc ggcatgccta
     5341 caacgtcctc cgggcggtga tgaacacagc ggtcgaggac aagctgatcg cagagaaccc
     5401 gtgccggatc gagcagaagg cagccgatga gcgcgacgta gaggcgctga cgcctgagga
     5461 gctggacatc gtcgccgctg agatcttcga gcactaccgg atcgcggcat acatcctggc
     5521 gtggacgagc ctccggttcg gagagctgat cgagcttcgc cgcaaggaca tcgtggacga
     5581 cggcatgacg atgaagctcc gggtgcgccg tggcgcttcc cgcgtgggga acaagatcgt
     5641 cgttggcaac gccaagaccg tccggtcgaa gcgtcctgtg acggttccgc ctcacgtcgc
     5701 ggagatgatc cgagcgcaca tgaagg
//
